標(biāo)題: Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁(yè)] 作者: 民俗學(xué) 時(shí)間: 2025-3-21 18:10
書(shū)目名稱(chēng)Drosophila影響因子(影響力)
作者: Thyroiditis 時(shí)間: 2025-3-22 00:02
Dark and Light Adaptation: Psychophysics,unction in . with a particular focus on the large-scale resources that enable systematic analysis. Application of these methods will accelerate the discovery of ways that miRNAs are embedded into genetic networks that control basic features of metazoan cells.作者: 硬化 時(shí)間: 2025-3-22 04:19
Models for Vector-Borne Parasitic Diseasesd from indirect in vivo techniques. Thus, this method provides an improved way of studying dynamic cellular processes and tissue movements during imaginal disc development. I first describe the preparation of the growth?medium and the dissection, and then I include a protocol for mounting and live imaging of the explants.作者: regale 時(shí)間: 2025-3-22 06:30
The Q-system: A Versatile Repressible Binary Expression System,plication of the Q-system for transgenic labeling, the modular nature of QF that allows chimeric or split transcriptional activators to be developed, its temporal control by quinic acid, new methods to generate QF2 reagents, intersectional expression labeling, and its recent adoption into many emerging experimental species.作者: Hangar 時(shí)間: 2025-3-22 11:15
Resources and Methods for the Analysis of MicroRNA Function in ,unction in . with a particular focus on the large-scale resources that enable systematic analysis. Application of these methods will accelerate the discovery of ways that miRNAs are embedded into genetic networks that control basic features of metazoan cells.作者: 誤傳 時(shí)間: 2025-3-22 13:39
Cultivation and Live Imaging of , Imaginal Discs,d from indirect in vivo techniques. Thus, this method provides an improved way of studying dynamic cellular processes and tissue movements during imaginal disc development. I first describe the preparation of the growth?medium and the dissection, and then I include a protocol for mounting and live imaging of the explants.作者: 誤傳 時(shí)間: 2025-3-22 21:00
1064-3745 ation advice from the expertsThis third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single作者: Loathe 時(shí)間: 2025-3-22 22:33
https://doi.org/10.1007/978-3-642-88653-9discovery. This chapter aims to give users new to the database an overview of the layout and types of data available, as well as introducing some tools with which to access the data. More experienced users will find useful information about recent improvements and descriptions to enable more efficient navigation of the database.作者: 勉強(qiáng) 時(shí)間: 2025-3-23 02:27
Affine und projektive Geometrierrors than traditional methods. Initially described in mammalian cells, prime editing is functional in . somatic and germ cells. Here, we outline steps to design, generate, and express prime editing components in transgenic flies. Furthermore, we highlight a crossing scheme to produce edited fly stocks in less than 3?months.作者: 閃光你我 時(shí)間: 2025-3-23 05:59
Rudolf Lapierre,Gerd Steierwald functional analyses. In this chapter, we describe the preparation of . tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing–freeze substitution (HPF–FS) to preserve cellular structures.作者: flavonoids 時(shí)間: 2025-3-23 13:21
Analysis of Single-Cell Transcriptome Data in ,q data. In this book chapter we want to give guidance on analyzing droplet-based scRNA-seq data from .. We will initially describe the preprocessing commonly done for ., point out possible downstream analyses, and finally highlight computational methods developed using . scRNA-seq data.作者: obviate 時(shí)間: 2025-3-23 14:58
CRISPR-Based Transcriptional Activation in ,ion domains are directly fused to dCas9 and MS2 phage Coat Protein (MCP), respectively, and the system in . is named flySAM. Here we describe the effective and convenient transcriptional activation system, flySAM, starting from vector construction, microinjection, and transgenic fly selection to the phenotypic analysis.作者: Charade 時(shí)間: 2025-3-23 20:05
Tracing and Manipulating , Cell Lineages Based on CRISPR: CaSSA and CLADES,hnology based on CRISPR that allows targeting and labeling specific lineage branches. Here we discuss how to better exploit this technology for lineage studies in ., with an emphasis on neuronal specification.作者: Barter 時(shí)間: 2025-3-23 23:55 作者: 駕駛 時(shí)間: 2025-3-24 06:10
Optochemical Control of Cell Contractility in , Embryos,erence with single-cell resolution and sub-minute timescale temporal control. Here we characterize an optochemical approach of Ca. uncaging to control cell contractility in . embryos. We describe in detail the method of sample preparation, microinjection, Ca. uncaging, and data analysis.作者: 切碎 時(shí)間: 2025-3-24 10:24 作者: 圣歌 時(shí)間: 2025-3-24 14:34 作者: 調(diào)情 時(shí)間: 2025-3-24 18:07
Die Nahrungsaufnahme bei Protozoenn protein binders in .. We describe their key features and provide an overview of the available reagents. Finally, we briefly explore the future avenues that protein binders might open up and thus further contribute to better understand development and homeostasis of multicellular organisms.作者: cathartic 時(shí)間: 2025-3-24 22:16 作者: SUGAR 時(shí)間: 2025-3-25 02:51 作者: Oratory 時(shí)間: 2025-3-25 04:57 作者: 鎮(zhèn)痛劑 時(shí)間: 2025-3-25 11:32 作者: 粗語(yǔ) 時(shí)間: 2025-3-25 14:02
Using FlyBase: A Database of , Genes and Genetics,discovery. This chapter aims to give users new to the database an overview of the layout and types of data available, as well as introducing some tools with which to access the data. More experienced users will find useful information about recent improvements and descriptions to enable more efficient navigation of the database.作者: 健忘癥 時(shí)間: 2025-3-25 19:43 作者: Affirm 時(shí)間: 2025-3-25 22:08
Preparation of , Tissues and Organs for Transmission Electron Microscopy, functional analyses. In this chapter, we describe the preparation of . tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing–freeze substitution (HPF–FS) to preserve cellular structures.作者: Blanch 時(shí)間: 2025-3-26 01:29
Vorlesungen über Geometrie der Algebrenq data. In this book chapter we want to give guidance on analyzing droplet-based scRNA-seq data from .. We will initially describe the preprocessing commonly done for ., point out possible downstream analyses, and finally highlight computational methods developed using . scRNA-seq data.作者: 混合,攙雜 時(shí)間: 2025-3-26 05:25
Die Verdauungsvorg?nge bei Protozoenion domains are directly fused to dCas9 and MS2 phage Coat Protein (MCP), respectively, and the system in . is named flySAM. Here we describe the effective and convenient transcriptional activation system, flySAM, starting from vector construction, microinjection, and transgenic fly selection to the phenotypic analysis.作者: Clumsy 時(shí)間: 2025-3-26 12:27 作者: 時(shí)代 時(shí)間: 2025-3-26 15:43 作者: 背書(shū) 時(shí)間: 2025-3-26 19:55 作者: 鍍金 時(shí)間: 2025-3-26 23:15 作者: Fibrin 時(shí)間: 2025-3-27 02:30 作者: REIGN 時(shí)間: 2025-3-27 08:44 作者: 橫條 時(shí)間: 2025-3-27 11:46
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/282971.jpg作者: 階層 時(shí)間: 2025-3-27 14:41 作者: canonical 時(shí)間: 2025-3-27 19:37
978-1-0716-2543-9The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 缺乏 時(shí)間: 2025-3-28 01:50 作者: 上下連貫 時(shí)間: 2025-3-28 03:20 作者: 離開(kāi) 時(shí)間: 2025-3-28 07:37
https://doi.org/10.1007/978-3-642-88653-9m research papers as well as computationally from a variety of relevant sources, to serve as an information hub that enables and accelerates research discovery. This chapter aims to give users new to the database an overview of the layout and types of data available, as well as introducing some tool作者: Lacerate 時(shí)間: 2025-3-28 13:44
Regulatory Aspects of Bioinert Substancessible binary expression system that consists of a transcription factor QF (and the recently improved QF2/QF2.), the inhibitor QS, a . effector, and a drug that inhibits QS (quinic acid). The Q-system can be used alone or in combination with other binary expression systems, such as GAL4/. and LexA/..作者: glucagon 時(shí)間: 2025-3-28 16:19 作者: ACRID 時(shí)間: 2025-3-28 19:05 作者: Nebulous 時(shí)間: 2025-3-29 02:01 作者: coltish 時(shí)間: 2025-3-29 04:43 作者: evanescent 時(shí)間: 2025-3-29 08:20
,Bet?ubungsmittelgesetz. (Opiumgesetz),y of these breakthroughs have been based on the identification of novel gene functions in large-scale genetic screens. However, conventional forward-genetic screens have been limited by the random nature of mutagenesis and difficulties in mapping causal mutations, while reverse-genetic RNAi screens 作者: Neonatal 時(shí)間: 2025-3-29 11:42
Die Verdauungsvorg?nge bei Protozoene transcription by introducing genes on exogenous vectors or by using the CRISPR/dCas9-based transcriptional activation system. To date, the most efficient CRISPR/dCas9-based transcriptional activator is the Synergistic Activation Mediator (SAM) system whereby three different transcriptional activat作者: reflection 時(shí)間: 2025-3-29 15:33
https://doi.org/10.1007/978-3-642-91037-1xtraordinary insight into the mechanisms underlying normal and pathological development. The analysis of molecular determinants involved in the acquisition of cell identity requires gaining experimental access to precise parts of cell lineages. Recently, we have developed CaSSA and CLADES, a new tec作者: arousal 時(shí)間: 2025-3-29 21:09
Die Nahrungsaufnahme bei Protozoenand homeostasis in .. In contrast to other techniques, that indirectly interfere with proteins via their nucleic acids (CRISPR, RNAi, etc.), protein binders permit direct and acute protein manipulation. Since the first use of a nanobody in . a decade ago, many different applications exploiting prote作者: intellect 時(shí)間: 2025-3-30 00:26
B. P. Babkin,G. Bergmann,R. Winklerdifferent from its target. This method induces the binding of the target protein to the anchor by either the addition of rapamycin to . food or cell media. Rapamycin mediates the formation of a ternary complex between the anchor, which is tagged with the FK506-binding protein (FKBP12), and the targe作者: hypnotic 時(shí)間: 2025-3-30 06:27 作者: 公社 時(shí)間: 2025-3-30 10:07 作者: Diskectomy 時(shí)間: 2025-3-30 13:24 作者: Apoptosis 時(shí)間: 2025-3-30 16:54
Zusammenstellung der benutzten Formelzeichenies of the basement membrane contribute to tissue elongation of the egg chamber. Here, we discuss how indentation with the microindenter of an atomic force microscope can be used to determine an effective stiffness value of a . egg chamber. We provide information on the preparation of egg chambers p作者: Metastasis 時(shí)間: 2025-3-30 22:43 作者: 慟哭 時(shí)間: 2025-3-31 01:15
Models for Vector-Borne Parasitic Diseases and metastatic behaviors. However, it has been challenging to analyze real-time cell interactions or cell dynamics within the epithelia under physiological conditions. The . pupal abdominal epidermis is a model system that allows to combine long-term real-time imaging under physiological conditions作者: aneurysm 時(shí)間: 2025-3-31 07:23
Rudolf Lapierre,Gerd Steierwaldl populations or entire tissues and the constantly improving microscopy technologies such as two-photon or light-sheet applications, with deep tissue imaging, hold great potential to address central biological questions at an organismic level. However, strong pigmentation and the opaque nature of th作者: 閑逛 時(shí)間: 2025-3-31 09:50
Rudolf Lapierre,Gerd Steierwaldlar complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of . tissues for TEM and provide reliable step-by-step protocols for applying class作者: 矛盾心理 時(shí)間: 2025-3-31 15:46
Optogenetic Methods to Control Tissue Mechanics in , application of this method to control tissue mechanics during . embryonic development. We detail optogenetic protocols to either increase or decrease cell contractility and analyze the interplay between cell–cell interaction, tissue geometry, and force transmission during gastrulation.作者: 挫敗 時(shí)間: 2025-3-31 18:46
Book 2022Latest editionell as tips on troubleshooting and avoiding known pitfalls.?Authoritative and cutting-edge,?.Drosophila: Methods and Protocols, Third Edition.?serves as a useful and practical guide to new researchers and experts using Drosophila as a model system..Chapter 7 is available open access under a Creative作者: 串通 時(shí)間: 2025-3-31 23:39 作者: 地牢 時(shí)間: 2025-4-1 03:44 作者: tangle 時(shí)間: 2025-4-1 06:36
Models for Vector-Borne Parasitic Diseasesaging of the abdominal epidermis. Moreover, methods to combine live imaging with mosaic analysis or drug injection will be presented. The long-term live imaging of the pupal abdominal epidermis is straightforward and opens up the possibility to analyze cell dynamics during epithelial morphogenesis a
