標(biāo)題: Titlebook: Directed Evolution; Methods and Protocol Andrew Currin,Neil Swainston Book 2022 Springer Science+Business Media, LLC, part of Springer Natu [打印本頁] 作者: Hemochromatosis 時(shí)間: 2025-3-21 19:04
書目名稱Directed Evolution影響因子(影響力)
書目名稱Directed Evolution影響因子(影響力)學(xué)科排名
書目名稱Directed Evolution網(wǎng)絡(luò)公開度
書目名稱Directed Evolution網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Directed Evolution被引頻次
書目名稱Directed Evolution被引頻次學(xué)科排名
書目名稱Directed Evolution年度引用
書目名稱Directed Evolution年度引用學(xué)科排名
書目名稱Directed Evolution讀者反饋
書目名稱Directed Evolution讀者反饋學(xué)科排名
作者: 永久 時(shí)間: 2025-3-21 21:27 作者: 諂媚于性 時(shí)間: 2025-3-22 01:24 作者: CUMB 時(shí)間: 2025-3-22 04:48
Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries,hods for multiple site-saturation mutagenesis, such as Darwin Assembly, can accelerate the sampling of relevant sequence space and the identification of variants with desired functionalities. Here, we present the automation of the Darwin Assembly method, using a Gilson PIPETMAX? liquid handling plat作者: Priapism 時(shí)間: 2025-3-22 09:19
SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Varprovements such as . thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein’s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address 作者: Tonometry 時(shí)間: 2025-3-22 13:35 作者: Tonometry 時(shí)間: 2025-3-22 17:36
GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes,or stability. In order to achieve these desired improvements, it is often beneficial to subject the entirety of the protein to mutagenesis. However, the creation of such libraries by targeted methods (i.e. site-directed mutagenesis) can be a laborious and costly task. Here we outline the GeneORator 作者: 新陳代謝 時(shí)間: 2025-3-23 00:37 作者: fringe 時(shí)間: 2025-3-23 02:45
Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs,ction of strong prokaryotic promoters that can be recognized by designated multiple sigma factors by interlocking their cognate binding motifs on DNA strands. Strong and stress responsive promoters for . and . have been created following the presented protocol. Customized promoters could be easily d作者: Femine 時(shí)間: 2025-3-23 07:42 作者: interrupt 時(shí)間: 2025-3-23 11:21 作者: 溫順 時(shí)間: 2025-3-23 14:50
Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity,tant metabolites, therefore presenting applications in the bioremediation, industrial biotechnology, and biomedical fields. The directed evolution of allosteric transcription factors (aTFs) with the aim of altering effector specificity has the potential for the development of new biosensors to detec作者: 偽造 時(shí)間: 2025-3-23 20:07
A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Deameter capillaries or micro- and nanofluidic channels through the application of a high voltage electric field. When capillary electrophoresis is assembled in a multicapillary instrument such as 96-well format (multiplexed), it becomes a powerful high-throughput system with the ability to simultaneo作者: Pudendal-Nerve 時(shí)間: 2025-3-23 23:41
Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screenic and industrial interest. Here we describe an ultra-high-throughput fluorescence activated cell sorting (FACS) method for the directed evolution of GTs, at the single cell level. This assay relies on the exquisite substrate specificity of lactose permeases (LacY) that are located in the cell membra作者: 寬宏大量 時(shí)間: 2025-3-24 04:11 作者: 不愛防注射 時(shí)間: 2025-3-24 07:24 作者: 音的強(qiáng)弱 時(shí)間: 2025-3-24 13:31 作者: 躺下殘殺 時(shí)間: 2025-3-24 16:16 作者: 攝取 時(shí)間: 2025-3-24 19:15 作者: 身心疲憊 時(shí)間: 2025-3-25 01:42 作者: cornucopia 時(shí)間: 2025-3-25 06:42 作者: 碎石 時(shí)間: 2025-3-25 09:53
https://doi.org/10.1007/978-3-322-95024-6or stability. In order to achieve these desired improvements, it is often beneficial to subject the entirety of the protein to mutagenesis. However, the creation of such libraries by targeted methods (i.e. site-directed mutagenesis) can be a laborious and costly task. Here we outline the GeneORator 作者: 為寵愛 時(shí)間: 2025-3-25 15:17 作者: HEAVY 時(shí)間: 2025-3-25 17:44
Husserliana: Edmund Husserl – Materialienction of strong prokaryotic promoters that can be recognized by designated multiple sigma factors by interlocking their cognate binding motifs on DNA strands. Strong and stress responsive promoters for . and . have been created following the presented protocol. Customized promoters could be easily d作者: Dysarthria 時(shí)間: 2025-3-25 22:24 作者: LITHE 時(shí)間: 2025-3-26 00:53
Walter Blauth,Hans-Wolfram Ulrichgenesis libraries in a PCR-independent manner. The plasmid DNA is double digested with Cas9 bearing specific single guide RNAs to remove the target nucleotides. Next, T5 exonuclease excises both 5′-ends of the linearized plasmid to generate homologous regions of approximately 15?nt. Subsequently, a 作者: 痛打 時(shí)間: 2025-3-26 07:54
Sp?tergebnisse in der Orthop?dietant metabolites, therefore presenting applications in the bioremediation, industrial biotechnology, and biomedical fields. The directed evolution of allosteric transcription factors (aTFs) with the aim of altering effector specificity has the potential for the development of new biosensors to detec作者: faction 時(shí)間: 2025-3-26 09:19
C. J. Wirth,M. J?ger,J. M. Schmidtameter capillaries or micro- and nanofluidic channels through the application of a high voltage electric field. When capillary electrophoresis is assembled in a multicapillary instrument such as 96-well format (multiplexed), it becomes a powerful high-throughput system with the ability to simultaneo作者: octogenarian 時(shí)間: 2025-3-26 13:22
https://doi.org/10.1007/978-3-642-65973-7c and industrial interest. Here we describe an ultra-high-throughput fluorescence activated cell sorting (FACS) method for the directed evolution of GTs, at the single cell level. This assay relies on the exquisite substrate specificity of lactose permeases (LacY) that are located in the cell membra作者: PANT 時(shí)間: 2025-3-26 17:03
https://doi.org/10.1007/978-3-642-65973-7armacological, and industrial applications. Synthetic biologists use iterative “design, build, test, and learn” cycles to efficiently engineer genetic systems that are reliable, reproducible, and predictable. Protein engineering by directed evolution can benefit from such a systematic engineering ap作者: 哀求 時(shí)間: 2025-3-26 22:21
Andrew Currin,Neil SwainstonIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 載貨清單 時(shí)間: 2025-3-27 04:26
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/280662.jpg作者: 新義 時(shí)間: 2025-3-27 05:50 作者: institute 時(shí)間: 2025-3-27 13:19 作者: fabricate 時(shí)間: 2025-3-27 15:08
Directed Evolution978-1-0716-2152-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Interim 時(shí)間: 2025-3-27 20:24 作者: 危機(jī) 時(shí)間: 2025-3-27 22:59 作者: 煩憂 時(shí)間: 2025-3-28 02:32 作者: Evolve 時(shí)間: 2025-3-28 07:45 作者: 鞭打 時(shí)間: 2025-3-28 11:55
Husserliana: Edmund Husserl – Materialienstrands. Strong and stress responsive promoters for . and . have been created following the presented protocol. Customized promoters could be easily developed for fine-tuning gene expression or overproducing enzymes with prokaryotic cell factories.作者: Brochure 時(shí)間: 2025-3-28 16:25 作者: LAVA 時(shí)間: 2025-3-28 21:34 作者: 擔(dān)心 時(shí)間: 2025-3-29 01:59 作者: doxazosin 時(shí)間: 2025-3-29 03:13
Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries,user-driven library-dependent programming of the liquid handling platform. We also present an approach for barcoding libraries that overcomes amplicon length limitations in next generation sequencing and enables fast reconstruction of library reads.作者: jettison 時(shí)間: 2025-3-29 11:05 作者: 沉默 時(shí)間: 2025-3-29 14:59
A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Dearies from directed evolution campaigns providing a comprehensive view on enzyme activity through the detection of all products formed. We describe the application of 96-MP-CE to screen mutant libraries of P450 BM3. MP-CE was used in directed evolution campaigns toward benzo-1,4-dioxane and α-isophorone.作者: cumber 時(shí)間: 2025-3-29 18:43
https://doi.org/10.1007/978-3-658-02720-9binatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.作者: 預(yù)兆好 時(shí)間: 2025-3-29 22:50
https://doi.org/10.1007/978-3-658-02720-9 14 mutated positions, a total of 16,384 library variants, and a protocol for the generation of large, user-defined combinatorial libraries. The reader can use this protocol to create such libraries in 2 days.作者: genesis 時(shí)間: 2025-3-30 01:37 作者: 生命層 時(shí)間: 2025-3-30 08:04
https://doi.org/10.1007/978-3-322-95024-6s can be quickly and efficiently cloned into a plasmid of interest, thereby accelerating directed evolution. On top of that, PTO-QuickStep can be utilized for rapid integration of noncoding DNA fragments to modify existing plasmids, making it an excellent tool for synthetic biologists.作者: ALTER 時(shí)間: 2025-3-30 11:17
https://doi.org/10.1007/978-3-642-71028-5to introduce circular permutation. Our RF cloning method made the protocol faster and more cost-effective. In this chapter, we describe a step-by-step protocol for generating circular permutants using RF methodology.作者: panorama 時(shí)間: 2025-3-30 15:05 作者: 解開 時(shí)間: 2025-3-30 20:20
SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Varbinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.作者: MELD 時(shí)間: 2025-3-30 22:41
Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis, 14 mutated positions, a total of 16,384 library variants, and a protocol for the generation of large, user-defined combinatorial libraries. The reader can use this protocol to create such libraries in 2 days.作者: 智力高 時(shí)間: 2025-3-31 01:17
GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes,mental workload. The method describes library synthesis using asymmetric PCR, in which mutagenic primers are designed to create OR-type mutations at multiple sites of variation in a two-step protocol. As an example, we show how this can be utilized for controlled and economical mutagenesis of every amino acid codon in a gene.作者: 我要沮喪 時(shí)間: 2025-3-31 05:21 作者: 怎樣才咆哮 時(shí)間: 2025-3-31 09:17
Application of Restriction Free (RF) Cloning in Circular Permutation,to introduce circular permutation. Our RF cloning method made the protocol faster and more cost-effective. In this chapter, we describe a step-by-step protocol for generating circular permutants using RF methodology.
