作者: 使人煩燥 時(shí)間: 2025-3-21 23:26 作者: VICT 時(shí)間: 2025-3-22 03:31 作者: palpitate 時(shí)間: 2025-3-22 04:51 作者: catagen 時(shí)間: 2025-3-22 08:48 作者: immunity 時(shí)間: 2025-3-22 16:07
Planung, Entscheidung und Kontrolle, can be implemented in high-throughput and thus are useful for biocatalyst discovery and engineering by directed evolution. These include assays employing Gibbs’ reagent, 4-aminoantipyrine (4-AAP) and Fast Violet B (FVB) (.). These methods should enable the optimization of oxygenases to industrially作者: immunity 時(shí)間: 2025-3-22 17:45
Book 2003rol environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evoluti作者: photopsia 時(shí)間: 2025-3-23 00:56 作者: ablate 時(shí)間: 2025-3-23 01:38 作者: cravat 時(shí)間: 2025-3-23 08:44 作者: 否決 時(shí)間: 2025-3-23 12:33 作者: Alveolar-Bone 時(shí)間: 2025-3-23 16:44 作者: 溺愛 時(shí)間: 2025-3-23 20:32
High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450 are easily modified for high-throughput in microtiter plates. This method is useful in quickly assaying a library of cytochrome P450s for folded and possibly functional proteins while eliminating misfolded or low expressing variants. It can also be used to rapidly determine the P450 concentration o作者: 瑪瑙 時(shí)間: 2025-3-23 23:38 作者: 逢迎白雪 時(shí)間: 2025-3-24 03:38 作者: 王得到 時(shí)間: 2025-3-24 08:16
Müfit Bahadir,Harun Parlar,Michael Spitelleren used in conjunction with directed molecular evolution. Our lab has used this approach to analyze the function of enzymes involved in DNA metabolism, to study the mutability of protein domains, and to generate mutant proteins possessing properties different from those selected by natural evolution作者: 思考 時(shí)間: 2025-3-24 12:56
Müfit Bahadir,Harun Parlar,Michael Spitellernditions is restored by small amounts of DNA polymerase activity. Even mutants with greatly reduced (1–10% of wild-type) catalytic activity or distantly-related polymerases of bacterial, eukaryotic, or viral origin effectively complement JS200 cells. The versatility of this complementation system ma作者: CREST 時(shí)間: 2025-3-24 15:54
Müfit Bahadir,Harun Parlar,Michael Spitellerymes such as DNA polymerases, which carry out pivotal role during DNA replication, repair, and recombination, are poorly conserved amongst different families, but within a given family, all the members are highly conserved. These observations have profound implications and suggest that DNA polymeras作者: Daily-Value 時(shí)間: 2025-3-24 19:50 作者: 可能性 時(shí)間: 2025-3-25 02:29
https://doi.org/10.1007/978-3-642-56998-2r its application as an industrial enzyme. The reason for low solubility can lie in low conformational stability (.), in a high number of surface-exposed hydrophobic amino acids (.) or in certain structural features, such as membrane binding regions (.). By changing the amino acid sequence of these 作者: 總 時(shí)間: 2025-3-25 06:11 作者: Malfunction 時(shí)間: 2025-3-25 09:36 作者: 頭腦冷靜 時(shí)間: 2025-3-25 12:48
Müfit Bahadir,Harun Parlar,Michael Spiteller,.). The first step is construction of a mutant library, usually accomplished by random point mutagenesis with error-prone PCR (.) or by DNA shuffling (recombination) (.,.). The second, and most critical step is finding the desired mutants by screening or selection of the libraries. Screens for enzy作者: nerve-sparing 時(shí)間: 2025-3-25 19:32 作者: inconceivable 時(shí)間: 2025-3-25 21:45
Springer-Handbuch der Mathematik Ie of its relative ease and high throughput compared to liquid-phase screening in multi-well plates. As with any high throughput screening approach, a suitable colorimetric or fluorimetric assay must exist (or be developed) for the enzyme function in question. There are two major requirements that mu作者: 痛恨 時(shí)間: 2025-3-26 01:40 作者: Urologist 時(shí)間: 2025-3-26 06:17 作者: TRACE 時(shí)間: 2025-3-26 08:36
Numerik und Wissenschaftliches Rechnen,(.). They contain a noncovalently bound protoporphyrin IX, giving these enzymes characteristic spectral properties. This heme has an available sixth coordination ligand that is able to bind carbon monoxide. Difference spectroscopy yields a spectral peak at approx 450 nm when comparing bound and unbo作者: crockery 時(shí)間: 2025-3-26 15:30
Planung, Entscheidung und Kontrolle,l aromatic oxidation reactions are prone to byproduct formation and often require heavy-metal catalysts, extremes of temperature and pressure, and explosive reagents (.). In contrast, biocatalysts such as mono- and dioxygenases perform the same chemistry in water at ambient conditions, usually with 作者: hurricane 時(shí)間: 2025-3-26 17:06 作者: commodity 時(shí)間: 2025-3-26 22:35
Grundlagen. Diskrete Mathematik,ic) substrates are used and no simple and reliable high-throughput method for quantitative analysis of the respective reaction product is available. The screening procedure should also be generally applicable for a certain class of enzymes. Generation of “surrogate substrates” by derivatization with作者: Focus-Words 時(shí)間: 2025-3-27 02:04
Numerische Mathematik und Programme,road variety of natural sources, most peroxidases use heme or vanadium as a cofactor at the redox active site, while some bacterial peroxidases function without a metal cofactor (.,.). Peroxidases catalyze a wide variety of oxidative reactions, some of which are used in industrial and biotechnologic作者: ineluctable 時(shí)間: 2025-3-27 08:34 作者: FLAGR 時(shí)間: 2025-3-27 09:56 作者: 思考 時(shí)間: 2025-3-27 17:01
Genetic Complementation Protocolsen used in conjunction with directed molecular evolution. Our lab has used this approach to analyze the function of enzymes involved in DNA metabolism, to study the mutability of protein domains, and to generate mutant proteins possessing properties different from those selected by natural evolution作者: 使厭惡 時(shí)間: 2025-3-27 21:17
Use of Pol I-Deficient , for Functional Complementation of DNA Polymerasenditions is restored by small amounts of DNA polymerase activity. Even mutants with greatly reduced (1–10% of wild-type) catalytic activity or distantly-related polymerases of bacterial, eukaryotic, or viral origin effectively complement JS200 cells. The versatility of this complementation system ma作者: 英寸 時(shí)間: 2025-3-27 23:21
Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeastymes such as DNA polymerases, which carry out pivotal role during DNA replication, repair, and recombination, are poorly conserved amongst different families, but within a given family, all the members are highly conserved. These observations have profound implications and suggest that DNA polymeras作者: idiopathic 時(shí)間: 2025-3-28 03:40
Autogene Selectionsas a dimensionality of 4., where n is the size of the nucleic acid pool (i.e., G, C, A, and T), protein sequence space has a dimensionality of 20.. Similarly, while nucleic acids can frequently be directly selected for function from a random sequence population, the corresponding methods for the dir作者: Mortal 時(shí)間: 2025-3-28 09:59
Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferaser its application as an industrial enzyme. The reason for low solubility can lie in low conformational stability (.), in a high number of surface-exposed hydrophobic amino acids (.) or in certain structural features, such as membrane binding regions (.). By changing the amino acid sequence of these 作者: grudging 時(shí)間: 2025-3-28 11:29 作者: 表否定 時(shí)間: 2025-3-28 15:37 作者: 粗語 時(shí)間: 2025-3-28 21:37 作者: Reverie 時(shí)間: 2025-3-29 00:31 作者: 惰性女人 時(shí)間: 2025-3-29 03:26
Solid-Phase Screening Using Digital Image Analysise of its relative ease and high throughput compared to liquid-phase screening in multi-well plates. As with any high throughput screening approach, a suitable colorimetric or fluorimetric assay must exist (or be developed) for the enzyme function in question. There are two major requirements that mu作者: 轎車 時(shí)間: 2025-3-29 07:28
Screening for Thermostabilityxquisite specificity and selectivity, they are often limited by insufficient stability. Improvements in enzyme activity through protein engineering often come at the cost of reduced stability. This is likely a result of both natural drift and a tradeoff that often exists between activity and stabili作者: 構(gòu)成 時(shí)間: 2025-3-29 15:16 作者: Spongy-Bone 時(shí)間: 2025-3-29 16:54
High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450(.). They contain a noncovalently bound protoporphyrin IX, giving these enzymes characteristic spectral properties. This heme has an available sixth coordination ligand that is able to bind carbon monoxide. Difference spectroscopy yields a spectral peak at approx 450 nm when comparing bound and unbo作者: probate 時(shí)間: 2025-3-29 22:40 作者: 禁令 時(shí)間: 2025-3-30 03:06 作者: Bureaucracy 時(shí)間: 2025-3-30 08:07
High-Throughput Screens Based on NAD(P)H Depletionic) substrates are used and no simple and reliable high-throughput method for quantitative analysis of the respective reaction product is available. The screening procedure should also be generally applicable for a certain class of enzymes. Generation of “surrogate substrates” by derivatization with作者: 毀壞 時(shí)間: 2025-3-30 11:41
High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidasesroad variety of natural sources, most peroxidases use heme or vanadium as a cofactor at the redox active site, while some bacterial peroxidases function without a metal cofactor (.,.). Peroxidases catalyze a wide variety of oxidative reactions, some of which are used in industrial and biotechnologic作者: carotid-bruit 時(shí)間: 2025-3-30 12:32 作者: Eructation 時(shí)間: 2025-3-30 17:55
High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidaseson without a metal cofactor (.,.). Peroxidases catalyze a wide variety of oxidative reactions, some of which are used in industrial and biotechnological applications. An activity assay that can be used in a high-throughput fashion becomes important for the engineering of new and improved peroxidases.作者: Ethics 時(shí)間: 2025-3-30 22:43 作者: 深淵 時(shí)間: 2025-3-31 02:51 作者: nonradioactive 時(shí)間: 2025-3-31 06:21
Müfit Bahadir,Harun Parlar,Michael Spiteller acids to stability are still in their infancy. Therefore, evolutionary methods for increasing protein stability are of great interest. In such approaches, large protein libraries are searched for stabilized variants by a screening or, ideally, a selection technique.作者: 冷淡一切 時(shí)間: 2025-3-31 12:07 作者: 鴿子 時(shí)間: 2025-3-31 17:20 作者: Eructation 時(shí)間: 2025-3-31 17:41 作者: CANON 時(shí)間: 2025-3-31 22:58 作者: CYT 時(shí)間: 2025-4-1 03:38
