標(biāo)題: Titlebook: Differential Display Methods and Protocols; Peng Liang,Arthur B. Pardee Book 19971st edition Humana Press 1997 [打印本頁] 作者: 側(cè)面上下 時間: 2025-3-21 17:23
書目名稱Differential Display Methods and Protocols影響因子(影響力)
書目名稱Differential Display Methods and Protocols影響因子(影響力)學(xué)科排名
書目名稱Differential Display Methods and Protocols網(wǎng)絡(luò)公開度
書目名稱Differential Display Methods and Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Differential Display Methods and Protocols被引頻次
書目名稱Differential Display Methods and Protocols被引頻次學(xué)科排名
書目名稱Differential Display Methods and Protocols年度引用
書目名稱Differential Display Methods and Protocols年度引用學(xué)科排名
書目名稱Differential Display Methods and Protocols讀者反饋
書目名稱Differential Display Methods and Protocols讀者反饋學(xué)科排名
作者: WITH 時間: 2025-3-21 23:51 作者: hallow 時間: 2025-3-22 04:24
Differential Display Using Random Hexamer-Primed cDNA, Motif Primers, and Agarose Gel Electrophoresintracellular signal transduction pathways. One cellular response to mitogenic stimulation is the sequential transcriptional induction of specific nuclear genes encoding proteins of diverse functions (reviewed in refs. . and .). Many of these proteins are likely to be required for DNA replication and作者: Affirm 時間: 2025-3-22 06:45 作者: 糾纏 時間: 2025-3-22 09:14 作者: 連累 時間: 2025-3-22 16:30 作者: 連累 時間: 2025-3-22 20:34 作者: 使隔離 時間: 2025-3-22 23:28
Differential Screening of Differential Display cDNA Products by Reverse Northernen made to improve and optimize the technique (.,.). One major bottle-neck remains, though, in the screening step for the verification of the cDNA fragments tentatively identified by differential display.作者: 的闡明 時間: 2025-3-23 05:05 作者: Immobilize 時間: 2025-3-23 07:50
Cloning of the 3′ Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Diff of a gene family, it is essential to isolate the representative cDNAs encoding the protein of interest. In the past, this has been especially difficult owing to highly similar protein coding region of the cDNAs from a gene family with minute variations in their amino acid composition. The 3′ noncod作者: 情感脆弱 時間: 2025-3-23 13:07
RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display is of prime biological interest. Accordingly, the concepts of the mRNA differential display (DD) and RNA fingerprinting techniques (.,.) promised a breakthrough in molecular biology, because they circumvented some severe limitations of traditional methods, such as differential screening and subtrac作者: 知識分子 時間: 2025-3-23 16:25 作者: Benzodiazepines 時間: 2025-3-23 18:31 作者: macabre 時間: 2025-3-23 23:34
Hormone-lnducible Genes in Prostate Cellsthe physiological maintenance of the gland can also depend on the presence of estrogens and other steroid hormones, retinoids and vitamin D (.). Steroid hormones transduce their biological effects via specific receptors that act as transcription factors. The androgen ligand-receptor complex, working作者: VICT 時間: 2025-3-24 04:17 作者: vertebrate 時間: 2025-3-24 08:19 作者: Enrage 時間: 2025-3-24 11:42 作者: 集聚成團(tuán) 時間: 2025-3-24 18:40 作者: tendinitis 時間: 2025-3-24 21:53
Scott Q. Harper,Pedro Gonzalez-Alegree the identification of differentially expressed messages are of particular interest and importance (for review, . ref. .). These new techniques have several unique advantages over the conventional methods such as differential and/or subtractive hybridization-based ones. They can compare a number of作者: Debate 時間: 2025-3-25 02:02
https://doi.org/10.1007/978-1-4939-7108-4y these genes will help understand not only gene function but also the underlying molecular mechanisms of a particular biological system. Differential display (DD) (.,.) and subtractive hybridization (SH) (review . refs. .–.) are by far the most common methods currently used by investigators for thi作者: parsimony 時間: 2025-3-25 07:02 作者: 拋棄的貨物 時間: 2025-3-25 08:15
Jun-Yang Liou,Bor-Sheng Ko,Tzu-Ching Changfalse positives as well as the time required for the process. Most importantly, the use of Northern blots with potentially contaminated probes is avoided and a streamlined direct sequencing protocol is used as an initial step. The individual methods presented here are not new, but have been put toge作者: flourish 時間: 2025-3-25 11:57
https://doi.org/10.1007/978-1-60761-769-3en made to improve and optimize the technique (.,.). One major bottle-neck remains, though, in the screening step for the verification of the cDNA fragments tentatively identified by differential display.作者: 鍍金 時間: 2025-3-25 17:45 作者: 1FAWN 時間: 2025-3-25 23:54 作者: Conjuction 時間: 2025-3-26 03:37 作者: Occipital-Lobe 時間: 2025-3-26 05:08 作者: 浪蕩子 時間: 2025-3-26 12:26
Dominik B. O. B?sl,Martina Bodes (cytokines) promote both the growth and differentiation of stem cells and committed progenitor cells. Cytokines bind and activate discrete receptors expressed on the surface of stem cells and thereby influence cell fate decisions.作者: 明確 時間: 2025-3-26 16:39
David Sim?es,Nuno Lau,Luís Paulo Reisthe physiological maintenance of the gland can also depend on the presence of estrogens and other steroid hormones, retinoids and vitamin D (.). Steroid hormones transduce their biological effects via specific receptors that act as transcription factors. The androgen ligand-receptor complex, working作者: Bouquet 時間: 2025-3-26 18:00
https://doi.org/10.1007/978-3-319-70836-2ar organism (.). Mechanisms are used by dividing cells to create asymmetries in their information content that then allow the emergence of heterogeneous cell types through differential gene expression (.,.). Most developmental processes share the following characteristics:作者: LINES 時間: 2025-3-26 21:23 作者: foppish 時間: 2025-3-27 04:27 作者: 指數(shù) 時間: 2025-3-27 08:01 作者: Infuriate 時間: 2025-3-27 13:02
https://doi.org/10.1007/978-3-319-70836-2ar organism (.). Mechanisms are used by dividing cells to create asymmetries in their information content that then allow the emergence of heterogeneous cell types through differential gene expression (.,.). Most developmental processes share the following characteristics:作者: Stricture 時間: 2025-3-27 14:21
Differential Screening of Differential Display cDNA Products by Reverse Northernen made to improve and optimize the technique (.,.). One major bottle-neck remains, though, in the screening step for the verification of the cDNA fragments tentatively identified by differential display.作者: Admonish 時間: 2025-3-27 20:55 作者: 浮夸 時間: 2025-3-27 23:39 作者: saturated-fat 時間: 2025-3-28 03:15 作者: 導(dǎo)師 時間: 2025-3-28 06:56 作者: Airtight 時間: 2025-3-28 14:19
Identification of Immediate—Early Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an in view of its important role in regulating cell growth and differentiation in response to polypeptide growth factors and also because constitutive activation of this pathway at level of Ras, Raf, or MEK can promote oncogenic transformation of mammalian cells (.,.). Since it is widely perceived tha作者: Inoperable 時間: 2025-3-28 16:26 作者: BOOM 時間: 2025-3-28 18:57
https://doi.org/10.1007/978-1-4939-7108-4tially expressed mRNAs (i.e., using plus/minus screening); but if the quest is to identify the majority of differentially expressed mRNA species, then a method that is not sensitive to mRNA abundance is required. To evaluate how well DD and SH can identify abundant as well as rare differentially exp作者: 體貼 時間: 2025-3-28 23:57 作者: mutineer 時間: 2025-3-29 06:48
Book 19971st editionmRNAs in a cell or tissue, in particular those with altered expression resulting from diff- ences in transcription or mRNA degradation. In contrast to conventional te- niques, DD can be used to compare mRNA expressions in many samples created under multiple experimental conditions. DD was conceived 作者: BADGE 時間: 2025-3-29 07:37
Differential Displayon and characterization of differentially expressed genes becomes one of the first steps toward the understanding of these important biological questions. Differential display (.) and a related RAP-PCR method (.) were developed to more efficiently identify and isolate these genes.作者: Defiance 時間: 2025-3-29 13:11
Direct Sequencing of Differential Display PCR Productsnchored primers, T.MN (M = G, A or C; N = G, A, T or C); and (2) PCR amplification of cDNA fragments using arbitrary 10-mer primers (upstream) and anchored downstream primers. One critical feature of this technique is to display most of the mRNA population on a sequencing gel after PCR.作者: anaerobic 時間: 2025-3-29 17:04
A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gelsther from a variety of sources in a revised order. Methods for the DD reverse transcription-polymerase chain reactions (RT-PCR) and electrophoresis conditions, for which we have used published methods (.) and the GenomyxLR DNA sequencer (.. and ref. .), are not covered here.作者: ostensible 時間: 2025-3-29 19:58 作者: CUMB 時間: 2025-3-30 01:59 作者: annexation 時間: 2025-3-30 07:36
RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Displayreakthrough in molecular biology, because they circumvented some severe limitations of traditional methods, such as differential screening and subtractive hybridization, during the analysis of differential gene expression.作者: SPASM 時間: 2025-3-30 08:35
Chao Huang,John Karijolich,Yi-Tao Yuon and characterization of differentially expressed genes becomes one of the first steps toward the understanding of these important biological questions. Differential display (.) and a related RAP-PCR method (.) were developed to more efficiently identify and isolate these genes.作者: Heterodoxy 時間: 2025-3-30 15:20 作者: freight 時間: 2025-3-30 19:16
Jun-Yang Liou,Bor-Sheng Ko,Tzu-Ching Changther from a variety of sources in a revised order. Methods for the DD reverse transcription-polymerase chain reactions (RT-PCR) and electrophoresis conditions, for which we have used published methods (.) and the GenomyxLR DNA sequencer (.. and ref. .), are not covered here.
