標(biāo)題: Titlebook: Difference Gel Electrophoresis; Methods and Protocol Kay Ohlendieck Book 2018 Springer Science+Business Media LLC 2018 DIGE.fluorescence im [打印本頁(yè)] 作者: 契約 時(shí)間: 2025-3-21 19:47
書(shū)目名稱Difference Gel Electrophoresis影響因子(影響力)
書(shū)目名稱Difference Gel Electrophoresis影響因子(影響力)學(xué)科排名
書(shū)目名稱Difference Gel Electrophoresis網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Difference Gel Electrophoresis網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Difference Gel Electrophoresis被引頻次
書(shū)目名稱Difference Gel Electrophoresis被引頻次學(xué)科排名
書(shū)目名稱Difference Gel Electrophoresis年度引用
書(shū)目名稱Difference Gel Electrophoresis年度引用學(xué)科排名
書(shū)目名稱Difference Gel Electrophoresis讀者反饋
書(shū)目名稱Difference Gel Electrophoresis讀者反饋學(xué)科排名
作者: manifestation 時(shí)間: 2025-3-21 22:35
DIGE-Based Phosphoproteomic Analysists DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accurate quantification of the differentially regulated phosphoproteins in biological samples.作者: 錯(cuò) 時(shí)間: 2025-3-22 03:51 作者: Debility 時(shí)間: 2025-3-22 07:11 作者: MUTED 時(shí)間: 2025-3-22 10:37
Book 2018cols. detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the high作者: 分解 時(shí)間: 2025-3-22 15:45 作者: 分解 時(shí)間: 2025-3-22 19:41 作者: Mutter 時(shí)間: 2025-3-22 23:46 作者: 踉蹌 時(shí)間: 2025-3-23 02:05 作者: 蹣跚 時(shí)間: 2025-3-23 08:56 作者: 浮雕 時(shí)間: 2025-3-23 10:26
Quadrilingual Economics Dictionaryh minimal or saturation labeling are routinely used. This chapter briefly describes the technical advantages of the pre-electrophoretic fluorescent labeling technique and discusses the bioanalytical usefulness of this highly successful electrophoretic method.作者: mortgage 時(shí)間: 2025-3-23 15:52 作者: 空洞 時(shí)間: 2025-3-23 19:40
B. Reulet,D. E. Prober,W. Belzigoled standard makes 2-D DIGE a highly accurate quantitative method enabling multiple protein samples to be separated on the same two-dimensional gel. The image matching and cross-gel statistical analysis generates robust quantitative results making data validation by independent technologies successful.作者: 未完成 時(shí)間: 2025-3-24 01:05
The Need for Quantitation in Cardiologypression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hemorrhage syndrome (LPHS) compared to noninfected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease.作者: 我就不公正 時(shí)間: 2025-3-24 03:59 作者: FAR 時(shí)間: 2025-3-24 07:47 作者: degradation 時(shí)間: 2025-3-24 13:36 作者: lobster 時(shí)間: 2025-3-24 16:50
Proteomic Analysis of Lung Tissue by DIGEpression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hemorrhage syndrome (LPHS) compared to noninfected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease.作者: conception 時(shí)間: 2025-3-24 21:10 作者: 串通 時(shí)間: 2025-3-24 23:18
Quadrilingual Economics Dictionarythe principles of 2D-PAGE and 2D-DIGE including limitations to the methods. 2D-PAGE and 2D-DIGE continue to be popular methods in bioprocessing-related research (particularly on recombinant Chinese hamster ovary cells), which will also be discussed in the review chapter.作者: 撤退 時(shí)間: 2025-3-25 05:57 作者: 挑剔小責(zé) 時(shí)間: 2025-3-25 08:32
https://doi.org/10.1007/978-94-009-8808-8olving sample preparation, protein extraction, differential fluorescence labeling using a 3-dye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, DIGE image analysis, protein digestion, and mass spectrometry.作者: conjunctivitis 時(shí)間: 2025-3-25 15:09
Two-Dimensional Gel Electrophoresis and 2D-DIGEthe principles of 2D-PAGE and 2D-DIGE including limitations to the methods. 2D-PAGE and 2D-DIGE continue to be popular methods in bioprocessing-related research (particularly on recombinant Chinese hamster ovary cells), which will also be discussed in the review chapter.作者: 否決 時(shí)間: 2025-3-25 16:28
Comparative Two-Dimensional Fluorescence Gel Electrophoresisl control of the x-dimension (p.), azo dyes can be used. Experiments are possible in both vertical and horizontal (h) electrophoresis devices, but hCoFGE is much easier to perform. For data analysis, commercial software capable of warping can be adapted.作者: exacerbate 時(shí)間: 2025-3-25 22:52
Comparative 3-Sample DIGE Analysis of Skeletal Musclesolving sample preparation, protein extraction, differential fluorescence labeling using a 3-dye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, DIGE image analysis, protein digestion, and mass spectrometry.作者: 摻假 時(shí)間: 2025-3-26 02:44 作者: colostrum 時(shí)間: 2025-3-26 07:09 作者: abracadabra 時(shí)間: 2025-3-26 11:23 作者: tangle 時(shí)間: 2025-3-26 14:58 作者: 肉體 時(shí)間: 2025-3-26 19:45
Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis of tissue protein compositions. Comparing tissue groups under different conditions is crucially important for advancing the biomedical field by characterization of cellular processes, understanding pathophysiological development and tissue biomarker discovery. This chapter discusses 2D-DIGE as a co作者: A保存的 時(shí)間: 2025-3-26 21:09 作者: Accord 時(shí)間: 2025-3-27 03:08 作者: 使堅(jiān)硬 時(shí)間: 2025-3-27 06:34
H. A. Snellen,H. C. Hemker,J. H. Bemmelities recorded at the different wavelengths are integrated and the ratio between volumes normalized to that of the internal standard. This provides an immediate appreciation of protein amount variations under the different conditions tested. In addition, proteins of interest can still be excised and作者: granite 時(shí)間: 2025-3-27 11:29
The Need for Quantitation in Cardiology of tissue protein compositions. Comparing tissue groups under different conditions is crucially important for advancing the biomedical field by characterization of cellular processes, understanding pathophysiological development and tissue biomarker discovery. This chapter discusses 2D-DIGE as a co作者: 純樸 時(shí)間: 2025-3-27 16:53 作者: 率直 時(shí)間: 2025-3-27 18:49 作者: aggrieve 時(shí)間: 2025-3-28 01:19
2D-DIGE and Fluorescence Image Analysisback of classical two-dimensional gel-electrophoresis. However, once 2D-gels are obtained, they must undergo a quite articulated multistep image analysis procedure before the final differential analysis via statistical mono- and multivariate methods. Here, the main steps of image analysis software a作者: 無(wú)思維能力 時(shí)間: 2025-3-28 02:44 作者: 粘連 時(shí)間: 2025-3-28 07:26
Native DIGE: Efficient Tool to Elucidate Protein Interactomesructural and functional heterogeneity to the proteome. One needs to consider this aspect while studying changes in abundance or activities of proteins in response to any physiological stimulus. Abundance changes in components of a given proteome can be best visualized and quantified using electropho作者: FAZE 時(shí)間: 2025-3-28 13:11
Comparative Two-Dimensional Fluorescence Gel Electrophoresist for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples, which need to be compared without the availability of replicates and thus cannot be studied using differential gel electrophoresis (DIGE). CoFGE corrects for gel-to-gel variability by co-running wit作者: 從屬 時(shí)間: 2025-3-28 14:57 作者: 滑動(dòng) 時(shí)間: 2025-3-28 20:24
DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) ins in formalin-fixed paraffin-embedded (FFPE) tissues and its use for biomarker discovery/identification of proteins that may contribute to cancer development and progression. Formalin fixation and paraffin embedding of tissue is the standard processing methodology practiced in pathology laboratori作者: prostatitis 時(shí)間: 2025-3-29 02:00 作者: 描繪 時(shí)間: 2025-3-29 03:49 作者: CANE 時(shí)間: 2025-3-29 10:41 作者: BARGE 時(shí)間: 2025-3-29 12:18 作者: grounded 時(shí)間: 2025-3-29 18:51 作者: 手段 時(shí)間: 2025-3-29 22:46
Proteomic Analysis of Lung Tissue by DIGEort not only gaseous exchange, but homeostasis and protection from infection. Guinea pigs are an important animal disease model for a number of infectious and noninfectious pulmonary conditions and the availability of a complete genome facilitates comprehensive analysis of tissues using the tools of作者: Debate 時(shí)間: 2025-3-30 00:50
Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE AnalysisD-DIGE) is a modified and advanced form of 2D gel electrophoresis. DIGE is a powerful biochemical method that compares two or three protein samples on the same analytical gel, and can be used to establish differentially expressed protein levels between healthy normal and diseased pathological tissue作者: engagement 時(shí)間: 2025-3-30 07:51
Quadrilingual Economics Dictionarye of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three fluorescent tags, offers greater sensitivity and reproducibility over conventional 2D-PAGE gels for d作者: Instrumental 時(shí)間: 2025-3-30 09:43 作者: 有角 時(shí)間: 2025-3-30 15:58 作者: 開(kāi)花期女 時(shí)間: 2025-3-30 17:36 作者: Cardioplegia 時(shí)間: 2025-3-30 20:50
https://doi.org/10.1007/978-94-009-8808-8ructural and functional heterogeneity to the proteome. One needs to consider this aspect while studying changes in abundance or activities of proteins in response to any physiological stimulus. Abundance changes in components of a given proteome can be best visualized and quantified using electropho作者: 排出 時(shí)間: 2025-3-31 02:30
Quadrilingual Economics Dictionaryt for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples, which need to be compared without the availability of replicates and thus cannot be studied using differential gel electrophoresis (DIGE). CoFGE corrects for gel-to-gel variability by co-running wit作者: GUISE 時(shí)間: 2025-3-31 08:02
Quadrilingual Economics Dictionary standard DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accur作者: 捕鯨魚(yú)叉 時(shí)間: 2025-3-31 11:45 作者: 歹徒 時(shí)間: 2025-3-31 15:33 作者: mortuary 時(shí)間: 2025-3-31 21:13 作者: Endemic 時(shí)間: 2025-4-1 00:10
B. Reulet,D. E. Prober,W. Belzigsment. It is based on two-dimensional gel electrophoresis (2-DE) separation of fluorescently labeled protein extracts. The tagging procedures are designed to not interfere with the chemical properties of proteins with respect to their p. and electrophoretic mobility, once a proper labeling protocol 作者: cluster 時(shí)間: 2025-4-1 04:33 作者: 衣服 時(shí)間: 2025-4-1 06:32
H. A. Snellen,H. C. Hemker,J. H. Bemmel electrophoresis) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their quantitative analyses. We can get global proteomic views using 2D DIGE within 3 days, and then identify proteins with differential expression levels us
