作者: Nonthreatening 時(shí)間: 2025-3-21 21:38
Use of Peptide Nucleic Acid Probes for Rapid Detection and Enumeration of Viable Bacteria in Recreaere detected by capturing chemiluminiscence on instant (e.g., Polaroid) films. Each viable cell (i.e., rRNA producing) is detected as a light spot from its microcolony on the film after scanning the image into a computer. This rapid . hybridization technique is simple and highly sensitive and could 作者: pantomime 時(shí)間: 2025-3-22 01:41
Detection of , in Various Sample Types Using Whole-Cell Fluorescent , Hybridization,dual cells without prior cultivation. In this chapter, the use of FISH for the detection and quantification of . in water samples and in the visualization of these bacteria inside protozoa and biofilms is described in detail.作者: Bronchial-Tubes 時(shí)間: 2025-3-22 08:22 作者: 違反 時(shí)間: 2025-3-22 12:49
Macrophage Cell Cultures for Rapid Isolation of Intracellular Bacteria,ned by a flow cytometry assay for expression of . chaperonin 10. The reduced time for isolation and identification of . (48–72 h) and the consistency of the test results make the use of macrophage cell lines attractive and cost-effective for veterinary laboratories involved in surveillance of bovine作者: Trypsin 時(shí)間: 2025-3-22 13:58
1064-3745 ed to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.978-1-61737-666-5978-1-59745-143-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Trypsin 時(shí)間: 2025-3-22 17:14
Book 2006Latest editionin monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.作者: 畸形 時(shí)間: 2025-3-22 23:49 作者: novelty 時(shí)間: 2025-3-23 03:26 作者: tattle 時(shí)間: 2025-3-23 09:08
https://doi.org/10.1007/978-3-030-03952-3dual cells without prior cultivation. In this chapter, the use of FISH for the detection and quantification of . in water samples and in the visualization of these bacteria inside protozoa and biofilms is described in detail.作者: parasite 時(shí)間: 2025-3-23 11:29
Hui-Yu Chen,Yi-Sheng Cheng,Hsiu-Hui Shihsing the genomic deoxyribonucleic acid template from several mycobacterial species and isolates. A selection of the remaining genes has been cloned and expressed in . and purified by affinity chromatography. Successfully purified proteins were analyzed using sera from rabbits immunized with .. Furth作者: dandruff 時(shí)間: 2025-3-23 17:56 作者: conspicuous 時(shí)間: 2025-3-23 20:47 作者: falsehood 時(shí)間: 2025-3-23 22:42 作者: catagen 時(shí)間: 2025-3-24 03:11 作者: lanugo 時(shí)間: 2025-3-24 08:13
Heat Shock Proteins in Neurosciencereaction is measured from the quenching (reduced fluorescence) of the reporter. The relative amount of a specific SNP allele is determined from the nucleotide incorporation rate in a thermocycling reaction. The quencher extension protocol presented was developed for SNP allele quantification in . and for microbial community analyses.作者: Amplify 時(shí)間: 2025-3-24 11:36
An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods,e is imaged using a charge-coupled device camera. Using the evanescent wave for excitation allows real-time imaging. Alternatively, a confocal scanner can also be used to detect and quantify fluorescent spots. A method for immobilizing capture antibodies, performing assays, and detecting bound targets is presented.作者: extinct 時(shí)間: 2025-3-24 18:11
Application of Two-Step Quantitative Reverse-Transcription PCR to Bacterial Diagnostics,gene opens a door to potential nonamplified direct detection technologies. In this chapter, a method is described to accurately quantify specific RNA transcripts and thus determine their potential utility as “high-copy” targets. The quantification method described also has application in gene-expression analysis.作者: GENRE 時(shí)間: 2025-3-24 20:59 作者: Myofibrils 時(shí)間: 2025-3-25 02:29
Alexzander A.A. Asea,Ian R. Brown-time polymerase chain reaction is described. This method is an attractive alternative to labor-intensive manual protocols and can easily be incorporated into the diagnostic microbiology laboratory workflow, with the ability to obtain results within 4 h.作者: 鉤針織物 時(shí)間: 2025-3-25 07:13
Detection of ,, and , in Blood and Cerebrospinal Fluid Using Fluorescence-Based PCR,ye. The method has the advantage that DNA extraction, liquid handling, PCR, and analysis also can be fully automated. In this chapter, the simultaneous detection of ., and . from clinical samples is described.作者: 的事物 時(shí)間: 2025-3-25 07:45
,Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler? for the Detection of Methi-time polymerase chain reaction is described. This method is an attractive alternative to labor-intensive manual protocols and can easily be incorporated into the diagnostic microbiology laboratory workflow, with the ability to obtain results within 4 h.作者: relieve 時(shí)間: 2025-3-25 14:06
Overview of DNA Purification for Nucleic Acid-Based Diagnostics From Environmental and Clinical Samlinical samples. The issues of sampling, sample preservation, separation of the microorganisms from the environmental or clinical matrix, and DNA purification are covered. This chapter will focus on the advantages and the disadvantages of the methods available.作者: 欄桿 時(shí)間: 2025-3-25 17:34
Detection of Verotoxin Genes , and , in , O157:H7 in Minced Beef Using Immunocapture and Real-Time lowed by immunomagnetic separation and extraction of deoxyribonucleic acid. Real-time polymearse chain reaction, using hybridization probes, is used to detect the verotoxin genes 1 and 2 found in . O157:H7. The assay has a detection limit of log. 3.5/mL . O157:H7 cells in minced beef.作者: 支柱 時(shí)間: 2025-3-25 23:00 作者: Angioplasty 時(shí)間: 2025-3-26 00:52 作者: 用不完 時(shí)間: 2025-3-26 04:42
Alexzander A. A. Asea,Punit Kaurgene opens a door to potential nonamplified direct detection technologies. In this chapter, a method is described to accurately quantify specific RNA transcripts and thus determine their potential utility as “high-copy” targets. The quantification method described also has application in gene-expression analysis.作者: 陪審團(tuán) 時(shí)間: 2025-3-26 09:53 作者: 出沒 時(shí)間: 2025-3-26 14:41 作者: endure 時(shí)間: 2025-3-26 18:21
Anjali Ramaswamy,Ping Wei,Fan Panleotide fingerprints), is based on high levels of polymorphism observed at several genomic loci in the . genomes that contain small tandemly repeated deoxyribonucleic sequences. The technique described is designed for medium- to high-throughput analyses. However, the method described can be modified to characterize fewer samples.作者: 艦旗 時(shí)間: 2025-3-26 22:53 作者: Fallibility 時(shí)間: 2025-3-27 04:09
A Review of Current and Future Molecular Diagnostic Tests for Use in the Microbiology Laboratory,. The use of these new molecular technologies is not restricted to detection and identification of microbial pathogens but also can be used for genotyping, allowing one to determine antibiotic resistance or to perform microbial fingerprinting.作者: Compatriot 時(shí)間: 2025-3-27 07:38 作者: 紡織品 時(shí)間: 2025-3-27 11:02
HOOF Prints,leotide fingerprints), is based on high levels of polymorphism observed at several genomic loci in the . genomes that contain small tandemly repeated deoxyribonucleic sequences. The technique described is designed for medium- to high-throughput analyses. However, the method described can be modified to characterize fewer samples.作者: 獸皮 時(shí)間: 2025-3-27 15:22 作者: 我悲傷 時(shí)間: 2025-3-27 21:09
Book 2006Latest editioncteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in lab作者: deriver 時(shí)間: 2025-3-27 23:20 作者: obnoxious 時(shí)間: 2025-3-28 05:23
Ganachari M. Nagaraja,Alexzander Asea provide an overview of of the various sample preparation approaches for bacteria for direct analyses (i.e., without culturing) in environmental and clinical samples. The issues of sampling, sample preservation, separation of the microorganisms from the environmental or clinical matrix, and DNA puri作者: 掃興 時(shí)間: 2025-3-28 06:23 作者: 拋射物 時(shí)間: 2025-3-28 11:48
Yi-Bing Ouyang,Lijun Xu,Rona G. Giffardices. Sandwich fluoroimmunoassays are performed on the surface of a patterned microscope slide that acts as an optical waveguide. Fluorescence from immunocomplexes formed on the slide surface is excited using the evanescent field, an electromagnetic component of light, and the pattern of fluorescenc作者: 得罪 時(shí)間: 2025-3-28 16:16 作者: 過于平凡 時(shí)間: 2025-3-28 19:07
Alexzander A.A. Asea,Ian R. Browncrobiological laboratory. Resistance to methicillin is encoded by the . gene in .. Because routine laboratory diagnostics may be time consuming and because species differentiation encounters a variety of difficulties, molecular techniques detecting both the . and a .-specific gene are used for rapid作者: 正面 時(shí)間: 2025-3-29 02:07 作者: prolate 時(shí)間: 2025-3-29 03:32 作者: Ambulatory 時(shí)間: 2025-3-29 08:13
Heat Shock Proteins in Neurosciencextension. A probe with a 5′-reporter is single-base extended with a dideoxy nucleotide containing a quencher if the target SNP allele is present. The reaction is measured from the quenching (reduced fluorescence) of the reporter. The relative amount of a specific SNP allele is determined from the nu作者: 蛤肉 時(shí)間: 2025-3-29 13:10
Maria Magdalena Barreca,Fabiana Geraciphenotypically and genotypically diverse species that comprises the classical nalidixic acid-resistant thermophilic campylobacters and the biochemical . variants, urease-positive, nalidixic acid-susceptible, and urease producing nalidixic acid-susceptible strains. AFLP profiling and whole-cell prote作者: limber 時(shí)間: 2025-3-29 15:56
Alexzander A. A. Asea,Punit Kaurhresholds. Although several molecular techniques have been developed to rapidly detect bacteria in complex matrices, the challenge to simultaneously detect and enumerate only viable cells remains a limiting factor to their routine application. This chapter describes the use of peroxidase-labeled pep作者: 事情 時(shí)間: 2025-3-29 22:53
Anjali Ramaswamy,Ping Wei,Fan Panhighly discriminating bacterial identification system that will reliably identify genetically related bacterial populations. For pathogenic bacteria with highly conserved genomes, such as the zoonotic pathogen ., finding distinguishing markers or traits for strain identification is challenging. This作者: cauda-equina 時(shí)間: 2025-3-30 00:54
https://doi.org/10.1007/978-3-030-03952-3ns with other microorganisms like protozoa are possible. Nosocomial legionellosis already has been linked frequently to .-contaminated artificial water supplies. For this reason, a rapid and accurate detection and quantification of these bacteria in environmental and clinical samples, combined with 作者: 分期付款 時(shí)間: 2025-3-30 07:26
Hui-Yu Chen,Yi-Sheng Cheng,Hsiu-Hui Shihproved diagnostic tests to detect . infection in these animals. As with many other animal diseases, efforts need to be concentrated on the development of simple, rapid, noninvasive tests that can be performed by veterinarians or animal producers without expensive laboratory equipment. With the genom作者: Rotator-Cuff 時(shí)間: 2025-3-30 08:24 作者: 單純 時(shí)間: 2025-3-30 15:05 作者: MORPH 時(shí)間: 2025-3-30 16:38 作者: FACET 時(shí)間: 2025-3-31 00:33
Louise O’ConnorIncludes supplementary material: 作者: Esalate 時(shí)間: 2025-3-31 04:21
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270644.jpg
