作者: NICE 時(shí)間: 2025-3-21 22:19 作者: 有害處 時(shí)間: 2025-3-22 01:06
Conrado C. Gon?alves,Carlos H. I. RamosThe technique of pulsed field gel electrophoresis (PFGE) enables researchers to separate linear DNA fragments of up to 10 Mb. The technique was first described by Schwartz and Cantor (.) and differs from conventional gel electrophoresis by requiring the DNA molecules periodically to change their direction of migration.作者: 智力高 時(shí)間: 2025-3-22 07:36
Conrado C. Gon?alves,Carlos H. I. RamosTotal DNA restriction pattern analysis is one of the techniques commonly used to identify and group bacteria (.–.). This technique can be used to separate species belonging to the same genus (.,.) or to distinguish highly similar bacterial strains within the same species or subspecies (.–.).作者: lavish 時(shí)間: 2025-3-22 10:32 作者: Blazon 時(shí)間: 2025-3-22 15:13
The Direct Immunofluorescent Filter Technique (DIFT),Many areas of analytical microbiology that deal with biological materials (e.g., medicine and the food, water, and pharmaceutical industries) need methods of detecting and identifying microorganisms that can give results in a few hours rather than the many days required for conventional methods.作者: Blazon 時(shí)間: 2025-3-22 17:19 作者: Fortify 時(shí)間: 2025-3-23 00:48 作者: 一加就噴出 時(shí)間: 2025-3-23 04:45
Antibiotic Resistance Profiling,a bacterium is tested for resistance, it should be compared, quantitatively with a standard. This standard may be the resistance of a wild-type strain, a typical strain, or a “type” strain, appropriate to the test strain.作者: 外貌 時(shí)間: 2025-3-23 07:23
Pyrolysis Mass Spectrometry,tra represent transient bacterial “fingerprints” that can be compared mathematically for relatedness. The method has potential for identification, classification, and typing of bacteria with a high degree of resolution and speed.作者: 引起 時(shí)間: 2025-3-23 11:02
Preparation of Bacterial Genomic DNA, chemical purification of the DNA. Two widely used methods for the preparation of bacterial DNA are those described by Marmur (.) and Kirby (.), but procedures are frequently modified to suit the particular organisms under study.作者: Exonerate 時(shí)間: 2025-3-23 17:29
Diagnostic Bacteriology Protocols978-1-59259-533-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 青春期 時(shí)間: 2025-3-23 20:51
https://doi.org/10.1007/978-4-431-55852-1a bacterium is tested for resistance, it should be compared, quantitatively with a standard. This standard may be the resistance of a wild-type strain, a typical strain, or a “type” strain, appropriate to the test strain.作者: DUST 時(shí)間: 2025-3-23 22:15
Alexzander A. A. Asea,Punit Kaurtra represent transient bacterial “fingerprints” that can be compared mathematically for relatedness. The method has potential for identification, classification, and typing of bacteria with a high degree of resolution and speed.作者: 雜色 時(shí)間: 2025-3-24 04:55
David A. Proia,Richard C. Bates chemical purification of the DNA. Two widely used methods for the preparation of bacterial DNA are those described by Marmur (.) and Kirby (.), but procedures are frequently modified to suit the particular organisms under study.作者: sterilization 時(shí)間: 2025-3-24 07:59
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270643.jpg作者: airborne 時(shí)間: 2025-3-24 13:37
https://doi.org/10.1007/978-4-431-55852-1a bacterium is tested for resistance, it should be compared, quantitatively with a standard. This standard may be the resistance of a wild-type strain, a typical strain, or a “type” strain, appropriate to the test strain.作者: jovial 時(shí)間: 2025-3-24 16:42
Tsuyoshi Hirota,Yoshitaka Fukada, bacteriocins are active in very low concentrations against specific strains. This property has been widely utilized for the identification of types of strains within several bacterial species and most methods are based on the inability of a strain (indicator) to grow on an agar surface that has pr作者: 壕溝 時(shí)間: 2025-3-24 21:47
HSF Is Required for Gametogenesisd this property can be exploited to construct highly discriminatory schemes for the type identification of strains in epidemiological studies. Part of the success of the technique is due to the simple methodology involved. Production and handling of bacteriophages requires nothing more than a compet作者: Bph773 時(shí)間: 2025-3-25 01:27
HSP60 as Modulators of Apoptosisative purposes and in the study of their protein biochemistry at the molecular level. The most popular of the techniques employed, discontinuous SDS-PAGE, was first described by Laemmli (.) as a method for the separation of polypeptides in complex mixtures and the determination of their molecular we作者: cochlea 時(shí)間: 2025-3-25 03:49
Exosomal Hsp60: A Tumor Biomarker?the site of biochemical reactions involved in respiration and oxidative phosphorylation, and the synthesis of structural membrane components. In contrast, the outer membrane forms a physical barrier between the inside of the bacterial cell and the external environment, and contains elements involved作者: 是剝皮 時(shí)間: 2025-3-25 07:52 作者: 使熄滅 時(shí)間: 2025-3-25 15:22
HSP60 as Modulators of Apoptosis over a range of mass-to-charge (m/z) values. Such data permit calculation of molecular weight and molecular structure. The technique has been used widely in microbiology for many years. Molecules are separated in a high vacuum electromagnetic field following ionization which traditionally was by el作者: ACME 時(shí)間: 2025-3-25 16:14 作者: CHECK 時(shí)間: 2025-3-25 20:37 作者: 有毒 時(shí)間: 2025-3-26 02:33 作者: preeclampsia 時(shí)間: 2025-3-26 06:53
Caio César Quini,Sunil Krishnanntification of such organisms has been done using conventional culture methods and biochemical techniques. These methods, although still considered by most to be the “gold standard” for microbial identification, can often be time consuming and laborious to perform. In addition, these methods can als作者: 有節(jié)制 時(shí)間: 2025-3-26 08:47
David A. Proia,Richard C. Bates chemical purification of the DNA. Two widely used methods for the preparation of bacterial DNA are those described by Marmur (.) and Kirby (.), but procedures are frequently modified to suit the particular organisms under study.作者: 幻想 時(shí)間: 2025-3-26 13:14
Conrado C. Gon?alves,Carlos H. I. Ramosencoding the bacterial diversity. They can be sequenced easily and, even when undetermined, their sequences can be quickly compared by molecular hybridization. In addition, short sequences on double-stranded DNA are recognized by restriction endonucleases as cleavage sites. The number and position o作者: 魯莽 時(shí)間: 2025-3-26 17:36
https://doi.org/10.1007/978-3-319-90725-3 created novel ways of detecting and identifying pathogenic microorganisms, which has led to a re-evaluation of methodological approaches in the diagnostic microbiology laboratory. At the same time, the move away from radioisotopes with all associated safety hazards has allowed DNA probe technology 作者: 幻影 時(shí)間: 2025-3-26 23:28 作者: aerobic 時(shí)間: 2025-3-27 02:02 作者: 詞匯表 時(shí)間: 2025-3-27 07:53
https://doi.org/10.1007/978-3-319-90725-3r isolating plasmids from bacteria belonging to a wide range of very different genera, e.g., . (.), . (.,.), and may be used as the basis of techniques for plasmid isolation from yet different species.作者: outrage 時(shí)間: 2025-3-27 10:39
Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens,e portion that accounts for its unique specificity, and it is this portion that possesses the antigen binding sites. This unique recognition of an antigen by an antibody is the principle that makes the immunoassay such a powerful diagnostic tool.作者: Range-Of-Motion 時(shí)間: 2025-3-27 15:40
Preparation of Bacterial Plasmid DNA,r isolating plasmids from bacteria belonging to a wide range of very different genera, e.g., . (.), . (.,.), and may be used as the basis of techniques for plasmid isolation from yet different species.作者: Genistein 時(shí)間: 2025-3-27 19:46 作者: 宿醉 時(shí)間: 2025-3-27 23:12
Determination of rRNA Gene Restriction Patterns,f these endonuclease-specific restriction sites on a DNA molecule determine the number and the sizes of the fragments generated by cleavage. The development of agarose gel electrophoresis for the separation of DNA fragments allows the comparison of restriction patterns of plasmid (.) and chromosomal DNA (.).作者: 最高點(diǎn) 時(shí)間: 2025-3-28 05:23 作者: Odyssey 時(shí)間: 2025-3-28 09:31
Bacteriocin Typing,of strains within several bacterial species and most methods are based on the inability of a strain (indicator) to grow on an agar surface that has previously supported the growth of the bacteriocin producer strain.作者: Soliloquy 時(shí)間: 2025-3-28 10:51 作者: fructose 時(shí)間: 2025-3-28 18:19
Analysis of Bacterial Outer Membrane Proteins,ast, the outer membrane forms a physical barrier between the inside of the bacterial cell and the external environment, and contains elements involved in the binding and transmembrane transportation of components required for bacterial biochemistry.作者: 保全 時(shí)間: 2025-3-28 19:43
Multilocus Enzyme Electrophoresis, of Selander and coworkers (.–.). Since then, numerous authors have been successfully applying MEE to an increasing number of bacteria. In MEE, cellular extracts are submitted to gel electrophoresis under nondenaturing conditions and enzymes are detected with specific staining methods (.,.).作者: Charitable 時(shí)間: 2025-3-28 23:08 作者: 流眼淚 時(shí)間: 2025-3-29 03:30
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format, most to be the “gold standard” for microbial identification, can often be time consuming and laborious to perform. In addition, these methods can also be complicated by the heterogeneous nature of food microflora and the subjective nature of many microbiological and biochemical techniques.作者: Anthrp 時(shí)間: 2025-3-29 11:15 作者: Fsh238 時(shí)間: 2025-3-29 12:11 作者: FADE 時(shí)間: 2025-3-29 15:58
Conrado C. Gon?alves,Carlos H. I. Ramosf these endonuclease-specific restriction sites on a DNA molecule determine the number and the sizes of the fragments generated by cleavage. The development of agarose gel electrophoresis for the separation of DNA fragments allows the comparison of restriction patterns of plasmid (.) and chromosomal DNA (.).作者: fluoroscopy 時(shí)間: 2025-3-29 22:47 作者: CREEK 時(shí)間: 2025-3-30 03:06
Tsuyoshi Hirota,Yoshitaka Fukadaof strains within several bacterial species and most methods are based on the inability of a strain (indicator) to grow on an agar surface that has previously supported the growth of the bacteriocin producer strain.作者: 使?jié)M足 時(shí)間: 2025-3-30 07:44
HSF Is Required for Gametogenesis the success of the technique is due to the simple methodology involved. Production and handling of bacteriophages requires nothing more than a competent aseptic technique and a basic understanding of their characteristics and life cycle.作者: 強(qiáng)制令 時(shí)間: 2025-3-30 10:05
Exosomal Hsp60: A Tumor Biomarker?ast, the outer membrane forms a physical barrier between the inside of the bacterial cell and the external environment, and contains elements involved in the binding and transmembrane transportation of components required for bacterial biochemistry.作者: Incise 時(shí)間: 2025-3-30 14:25
HSP60: A Story as Long as Life on the Earth of Selander and coworkers (.–.). Since then, numerous authors have been successfully applying MEE to an increasing number of bacteria. In MEE, cellular extracts are submitted to gel electrophoresis under nondenaturing conditions and enzymes are detected with specific staining methods (.,.).作者: Ablation 時(shí)間: 2025-3-30 19:35
Jeremie Vitte,Marco Giovannini MD, PhDproblems to be identified soon enough to allow the appropriate remedial action to be implemented. New methods must produce rapid, accurate, and reliable estimates of total viable microbial numbers, spoilage, or indicator organisms or to identify the presence of pathogens.作者: 持久 時(shí)間: 2025-3-30 20:52
Caio César Quini,Sunil Krishnan most to be the “gold standard” for microbial identification, can often be time consuming and laborious to perform. In addition, these methods can also be complicated by the heterogeneous nature of food microflora and the subjective nature of many microbiological and biochemical techniques.作者: 引導(dǎo) 時(shí)間: 2025-3-31 01:08 作者: 神圣不可 時(shí)間: 2025-3-31 05:07 作者: HEAVY 時(shí)間: 2025-3-31 11:12
Bacteriocin Typing,, bacteriocins are active in very low concentrations against specific strains. This property has been widely utilized for the identification of types of strains within several bacterial species and most methods are based on the inability of a strain (indicator) to grow on an agar surface that has pr作者: animated 時(shí)間: 2025-3-31 13:26
Bacteriophage Typing,d this property can be exploited to construct highly discriminatory schemes for the type identification of strains in epidemiological studies. Part of the success of the technique is due to the simple methodology involved. Production and handling of bacteriophages requires nothing more than a compet作者: 認(rèn)識(shí) 時(shí)間: 2025-3-31 20:20
The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis,ative purposes and in the study of their protein biochemistry at the molecular level. The most popular of the techniques employed, discontinuous SDS-PAGE, was first described by Laemmli (.) as a method for the separation of polypeptides in complex mixtures and the determination of their molecular we作者: intolerance 時(shí)間: 2025-3-31 23:41 作者: anesthesia 時(shí)間: 2025-4-1 04:43
Multilocus Enzyme Electrophoresis,tly that the usefulness of MEE in bacterial genetics, systematics, and epidemiology, has been clearly demonstrated, essentially by the pioneering work of Selander and coworkers (.–.). Since then, numerous authors have been successfully applying MEE to an increasing number of bacteria. In MEE, cellul作者: 一夫一妻制 時(shí)間: 2025-4-1 06:39 作者: Acetaldehyde 時(shí)間: 2025-4-1 13:22 作者: 休息 時(shí)間: 2025-4-1 14:30
The Direct Epifluorescent Filter Technique (DEFT),ults within a few hours. The many days needed for conventional enrichment, plating, and biochemical/serological methods to give results do not enable problems to be identified soon enough to allow the appropriate remedial action to be implemented. New methods must produce rapid, accurate, and reliab作者: Banister 時(shí)間: 2025-4-1 19:31
Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens,ed into an animal, will elicit an antibody response. Antigens may be proteins, polypeptides, or carbohydrates (glycolipids or glycoproteins), and each antigen may contain one, or numerous, antigenic determinants or epitopes (.). The antibody molecules that are produced as a result of exposure to the作者: arboretum 時(shí)間: 2025-4-2 00:35
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format,ntification of such organisms has been done using conventional culture methods and biochemical techniques. These methods, although still considered by most to be the “gold standard” for microbial identification, can often be time consuming and laborious to perform. In addition, these methods can als
