標(biāo)題: Titlebook: Diabetes Mellitus; Sabire ?zcan Book 2003 Humana Press 2003 diabetes [打印本頁(yè)] 作者: Goiter 時(shí)間: 2025-3-21 19:03
書目名稱Diabetes Mellitus影響因子(影響力)
書目名稱Diabetes Mellitus影響因子(影響力)學(xué)科排名
書目名稱Diabetes Mellitus網(wǎng)絡(luò)公開度
書目名稱Diabetes Mellitus網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Diabetes Mellitus被引頻次
書目名稱Diabetes Mellitus被引頻次學(xué)科排名
書目名稱Diabetes Mellitus年度引用
書目名稱Diabetes Mellitus年度引用學(xué)科排名
書目名稱Diabetes Mellitus讀者反饋
書目名稱Diabetes Mellitus讀者反饋學(xué)科排名
作者: 聽寫 時(shí)間: 2025-3-21 23:59
Book 2003l tool for basic researchers and clinicians for investigating and treating this disease. Each chapter starts with an introduction to a specific technique and explains its application in the field of diabetes research. Following the list of materials, a detailed description of the technique is presen作者: Increment 時(shí)間: 2025-3-22 01:08 作者: formula 時(shí)間: 2025-3-22 06:05
A Satisfiability Solving Approach not removed from the cleavage site. “Des” proinsulin 64–65 and 31–32 are produced when cleavage is incomplete and the basic amino acid pairs are removed from the cleavage site (.). In the rat, two separate 110-amino acid preproinsulins are transcribed from two nonallelic preproinsulin genes, from w作者: Insubordinate 時(shí)間: 2025-3-22 12:37 作者: GRUEL 時(shí)間: 2025-3-22 14:27 作者: GRUEL 時(shí)間: 2025-3-22 18:40
Terry Lamb,Aniko Hatoss,Shirley O’Neill activities. These complexes do not generally possess DNA-binding activity, but are recruited to promoters by their interaction with sequence-specific transcription factors (.). The resulting modifications of the histone tails, in turn, modulate the access of further regulatory complexes to the prom作者: 使堅(jiān)硬 時(shí)間: 2025-3-22 21:21 作者: 流逝 時(shí)間: 2025-3-23 02:57
Erwin Blackstone,Simon Hakim,Brian J. Meehan. Therefore, we have recently established electroporation conditions that consistently provide at least 50% transfection efficiency for cultured differentiated 3T3-L1 adipocytes (.). Although the electroporation is not 100% efficient, it provides an easy and fast method to introduce DNA into adipocy作者: 文件夾 時(shí)間: 2025-3-23 08:23 作者: 傷心 時(shí)間: 2025-3-23 13:32
Natural Radiation and Environmentrelies on sonic disruption of cells that are coated with a positively charged molecule, which allows the exofacial surface of the PM to be drawn into contact with the solid support on which the cells were cultured. Sonication of these cells results in formation of a “l(fā)awn” of adherent membrane fragm作者: 珍奇 時(shí)間: 2025-3-23 15:41 作者: tooth-decay 時(shí)間: 2025-3-23 19:58
Nagesh Bhat,Rajesh K. Chaurasia,Usha Yadavnown. PtdIns(4,5)P. can be produced by the action of PtdIns 4- or 5-kinases on PtdIns(5)P or PtdIns(4)P, respectively, and is involved in signaling via G-protein-coupled receptors, regulating vesicle-mediated protein traffic, and actin filament polymerization. PtdIns(4,5)P. also serves as a precurso作者: 勛章 時(shí)間: 2025-3-24 00:57
Isolation of Islets of Langerhans from Rodent Pancreaspment of transgenic and gene knockout models, mouse islets represent an ideal system to study specific changes in gene expression on β-cell function. In this chapter, the methods that we routinely use to isolate islets from rat and mouse pancreata are described.作者: FEIGN 時(shí)間: 2025-3-24 04:47
Assessment of Insulin Secretion in the Mouse not removed from the cleavage site. “Des” proinsulin 64–65 and 31–32 are produced when cleavage is incomplete and the basic amino acid pairs are removed from the cleavage site (.). In the rat, two separate 110-amino acid preproinsulins are transcribed from two nonallelic preproinsulin genes, from w作者: 船員 時(shí)間: 2025-3-24 10:17
Detection of Insulin Production by Immunohistochemistrywo-well, four-well, or eight-well chamber slides are recommended (available from several commercial sources, including Nunc), as these allow rapid and simple processing of large numbers of replicate samples, with small volumes of antibody. However, the following protocol can also be applied to cells作者: Pathogen 時(shí)間: 2025-3-24 11:54
Quantification of the Level of Insulin Gene Expression technique and there are several commercial RNA preparation kits available (e.g., RNeasy from Qiagen). Such commercially available kits can also be used to isolate poly-A. RNA (mRNA) only, which assists in decreasing background signals. To investigate both rapid and long-term effects on insulin gene作者: 繁忙 時(shí)間: 2025-3-24 15:57 作者: 兇猛 時(shí)間: 2025-3-24 22:00
Utilization of NOD Mice in the Study of Type 1 Diabetesgh or low fasting plasma glucoses. Paradoxically, the diabetic mice were derived from the low-fasting-glucose line and a diabetes-resistant mouse, NON, was derived from the high-fasting-glucose line. The NON mouse, genetically similar to the NOD mouse, served as a nondiabetic control mouse strain. T作者: 鞠躬 時(shí)間: 2025-3-25 02:56
Introduction of DNA into 3T3-L1 Adipocytes by Electroporation. Therefore, we have recently established electroporation conditions that consistently provide at least 50% transfection efficiency for cultured differentiated 3T3-L1 adipocytes (.). Although the electroporation is not 100% efficient, it provides an easy and fast method to introduce DNA into adipocy作者: conformity 時(shí)間: 2025-3-25 07:22
Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytestranslocation because complete inhibition of GLUT-4 endocytosis only modestly increases plasma membrane-associated GLUT-4 protein without affecting the extent of insulin-stimulated GLUT-4 translocation (.–.). In contrast to GLUT-4, GLUT-1 is an intracellular and plasma membrane localized in the basa作者: LATHE 時(shí)間: 2025-3-25 09:06
Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assayrelies on sonic disruption of cells that are coated with a positively charged molecule, which allows the exofacial surface of the PM to be drawn into contact with the solid support on which the cells were cultured. Sonication of these cells results in formation of a “l(fā)awn” of adherent membrane fragm作者: extemporaneous 時(shí)間: 2025-3-25 13:55
Assaying AKT/Protein Kinase B Activitytissue extracts by quantifying its ability to catalyze phosphate incorporation into an exogenous substrate (outlined in .). This chapter illustrates techniques for immunoprecipitating Akt/PKB, choosing an appropriate substrate for the reaction, and optimizing the assay conditions.作者: 我吃花盤旋 時(shí)間: 2025-3-25 16:05 作者: 指耕作 時(shí)間: 2025-3-25 21:37
Christoph Schwindt,Jürgen Zimmermann. Islets are comprised of a heterogeneous population of endocrine cells, including insulin-producing β-cells (approx. 65–70%), glucagon-secreting α-cells (20–25%), somatostatin-secreting δ-cells, and polypeptide (PP)-secreting cells. Much of the cellular and biochemical information concerning the me作者: Cursory 時(shí)間: 2025-3-26 01:46 作者: Tdd526 時(shí)間: 2025-3-26 08:09 作者: Affiliation 時(shí)間: 2025-3-26 10:19
Lower Bounds and Exact Solution Approaches.), β-cells in culture (.), tissue samples (.), and cells transgenically expressing the insulin gene (.). Like all immunocytochemistry techniques, much depends on the availability of a high-quality primary antibody. Species-specific insulin antibodies are now available from a number of commercial so作者: 尖牙 時(shí)間: 2025-3-26 16:36
Duquesne Collective Action Network mRNA levels in both β-cell lines and purified islets has been performed by Northern blot analysis, using the full-length insulin cDNA as a labeled probe (.). This technique is described here, and is still the most useful method for detection of endogenous insulin mRNA levels (densitometry being app作者: extemporaneous 時(shí)間: 2025-3-26 18:49
Terry Lamb,Aniko Hatoss,Shirley O’Neillapproaches has become more important in light of the emerging notion that the natural chromatin context affects the outcome of transcriptional activation in vivo (.). Chromatin can exert a regulatory effect on transcription by modulating the access of activators to DNA (.). Different posttranslation作者: Ingratiate 時(shí)間: 2025-3-26 22:53 作者: 輪流 時(shí)間: 2025-3-27 03:48
Private Law Enforcement in New York City1 diabetes. These models have many features of human type 1 diabetes with some important exceptions that will be pointed out in this chapter. The animal models include both spontaneous disease models as well as induced diabetes such as streptozotocin-induced diabetes. One of the most widely used spo作者: Ancillary 時(shí)間: 2025-3-27 08:12 作者: Stable-Angina 時(shí)間: 2025-3-27 13:14 作者: 分開 時(shí)間: 2025-3-27 14:35 作者: 恃強(qiáng)凌弱 時(shí)間: 2025-3-27 20:44
Natural Radiation and Environment with antibodies or chemical probes. This method was first reported by Robinson et al. (.) who used it to detect the presence of the GLUT-4 glucose transporter at the PM as a result of insulin stimulation. We have extended this technique in order to use it as a means to quickly and efficiently obtai作者: 我們的面粉 時(shí)間: 2025-3-28 00:50
Handbook on Radiation Environment, Volume 1oxyl position of phosphoinositides to generate the intracellular messengers phosphatidylinositol-3-phosphate [PI(3)P], phosphatidylinositol-3,4-bisphos-phate [PI(3,4)P.], and phosphatidylinositol-3,4,5-trisphosphate [PI(3,4,5)P.] (reviewed in ..). PI3K commonly participates in signaling pathways lea作者: preeclampsia 時(shí)間: 2025-3-28 04:44 作者: affect 時(shí)間: 2025-3-28 06:27
Nagesh Bhat,Rajesh K. Chaurasia,Usha Yadav identified in eukaryotic cells (all are found in 3T3-L1 cells), including phosphatidylinositol 3-phosphate [PtdIns(3)P], phosphatidylinositol 4-phosphate [PtdIns(4)P], phosphatidylinositol 5-phosphate [PtdIns(5)P], phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P.], phosphatidylinositol 3,5-bisp作者: floaters 時(shí)間: 2025-3-28 12:49
https://doi.org/10.1007/0-306-48403-X glycogen metabolizing enzymes (.). Insulin binds to its receptor in peripheral tissues and initiates several signaling cascades to increase glucose uptake via translocation of the glucose tranporter-4 (GLUT-4) containing vesicles to the plasma membrane (..). Glucose enters the cells and is phosphor作者: 使害羞 時(shí)間: 2025-3-28 15:34
Nishant Chaudhary,Archana SharmaThis chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest stages.作者: 宣傳 時(shí)間: 2025-3-28 20:43
Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor SubstratesThis chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest stages.作者: 你不公正 時(shí)間: 2025-3-29 00:29
Sabire ?zcanIncludes supplementary material: 作者: APO 時(shí)間: 2025-3-29 04:27 作者: expository 時(shí)間: 2025-3-29 09:29 作者: 合同 時(shí)間: 2025-3-29 14:44
A Satisfiability Solving Approachw, intercellular communication in islets is likely to change as well. Furthermore, contamination of islets with anatomically associated acinar cells is inevitable during isolation and may have a major influence on the specificity of experiments.作者: 憤怒事實(shí) 時(shí)間: 2025-3-29 16:10 作者: TIA742 時(shí)間: 2025-3-29 22:21 作者: COMMA 時(shí)間: 2025-3-30 01:29
Purification of Rat Pancreatic β-Cells by Fluorescence-Activated Cell Sortingw, intercellular communication in islets is likely to change as well. Furthermore, contamination of islets with anatomically associated acinar cells is inevitable during isolation and may have a major influence on the specificity of experiments.作者: EVADE 時(shí)間: 2025-3-30 07:36
Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity PI3K is an obligate intermediate in the signaling pathway linking insulin’s arrival at the cell surface to the regulation of glucose uptake and anabolic metabolism (reviewed in ..). This chapter describes a method for quantifying the activation of the class 1A type PI3Ks by insulin.作者: abject 時(shí)間: 2025-3-30 11:28
Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytessis or be metabolized to uridine diphospho (UDP)-glucose and stored as glycogen. Measurement of glycogen synthesis rates in cells and tissues is thus a powerful way to examine several insulin metabolic responses simultaneously.作者: 易怒 時(shí)間: 2025-3-30 13:42
Book 2003ulin p- duction, insulin action, or both. About 6. 2% of the US population (17 million people) have diabetes mellitus. It is the leading cause of kidney failure, bli- ness, and amputations. It is also a major risk factor for heart diseases, stroke, and birth defects. Diabetes Mellitus: Methods and P作者: critic 時(shí)間: 2025-3-30 19:45 作者: 辮子帶來(lái)幫助 時(shí)間: 2025-3-30 21:09
Quality evaluation for e-learning in Europeage to various tissues are unknown. However, it is known that chronic hyperglycemia results in acute metabolic abnormalities such as insulin resistance that leads to worsening of diabetes (.). Insulin resistance results in decreased insulin-stimulated glucose transport into skeletal muscle and adipocyte tissue (.).作者: AXIS 時(shí)間: 2025-3-31 02:32
Handbook on Public and Private Securityenes of interest (.). Adenoviral gene transfer is also important in gene therapy of many inherited disorders (.). Recombinant adenoviruses are commonly used for the overexpression of transgenes and can also be used to downregulate genes of interest through the expression of antisense ribozymes and Cre-recombinase (.,.).作者: Reverie 時(shí)間: 2025-3-31 05:15 作者: hypotension 時(shí)間: 2025-3-31 11:23
Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytesage to various tissues are unknown. However, it is known that chronic hyperglycemia results in acute metabolic abnormalities such as insulin resistance that leads to worsening of diabetes (.). Insulin resistance results in decreased insulin-stimulated glucose transport into skeletal muscle and adipocyte tissue (.).作者: 點(diǎn)燃 時(shí)間: 2025-3-31 13:40
Isolation of Islets of Langerhans from Rodent Pancreas. Islets are comprised of a heterogeneous population of endocrine cells, including insulin-producing β-cells (approx. 65–70%), glucagon-secreting α-cells (20–25%), somatostatin-secreting δ-cells, and polypeptide (PP)-secreting cells. Much of the cellular and biochemical information concerning the me作者: prosperity 時(shí)間: 2025-3-31 20:35
Purification of Rat Pancreatic β-Cells by Fluorescence-Activated Cell Sortingc effects of individual factors on β-cell function. To preserve the microenvironment of the β-cell, most studies of β-cell physiology have been performed on in vitro cultured islets of Langerhans. However, whereas islets in the pancreas are highly vascularized and oxygenated, ischemic conditions can作者: 性上癮 時(shí)間: 2025-4-1 01:02
Assessment of Insulin Secretion in the Mouseed by removal of the amino terminal, 24-amino acid signal sequence (.). The resulting 86-amino acid product folds through the formation of three disulfide bridges between Cys.-Cys., Cys.-Cys., and Cys.-Cys. to produce the prohormone, proinsulin. Insulin and C-peptide are produced when endopeptidases作者: 攀登 時(shí)間: 2025-4-1 02:44
Detection of Insulin Production by Immunohistochemistry.), β-cells in culture (.), tissue samples (.), and cells transgenically expressing the insulin gene (.). Like all immunocytochemistry techniques, much depends on the availability of a high-quality primary antibody. Species-specific insulin antibodies are now available from a number of commercial so作者: commute 時(shí)間: 2025-4-1 08:22 作者: 綁架 時(shí)間: 2025-4-1 11:39 作者: PLUMP 時(shí)間: 2025-4-1 17:23
Adenoviral Gene Transfer into β-Cell Lineson are tightly regulated in order to maintain a well state. Alterations in these processes can lead to diseased states such as diabetes mellitus. In order to understand the complexity of these processes, it is imperative that scientists can alter the levels of expression of various genes in order to
