標(biāo)題: Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014 [打印本頁] 作者: Kennedy 時間: 2025-3-21 18:30
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書目名稱Developmental Biology of the Sea Urchin and Other Marine Invertebrates影響因子(影響力)學(xué)科排名
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書目名稱Developmental Biology of the Sea Urchin and Other Marine Invertebrates讀者反饋
書目名稱Developmental Biology of the Sea Urchin and Other Marine Invertebrates讀者反饋學(xué)科排名
作者: aerobic 時間: 2025-3-22 00:18 作者: 吵鬧 時間: 2025-3-22 01:45
Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses,o three types, cyphonautes (shelled, feeding), pseudocyphonautes (shelled, nonfeeding), and coronate (unshelled, nonfeeding), although each group is heterogeneous and probably includes various morphologies that are largely undescribed. Here, methods for rearing bryozoan colonies and larvae are presented.作者: 能夠支付 時間: 2025-3-22 08:22 作者: DAMN 時間: 2025-3-22 12:07 作者: GIST 時間: 2025-3-22 15:05
Perturbations to the Hedgehog Pathway in Sea Urchin Embryos,hods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.作者: GIST 時間: 2025-3-22 20:29
Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nmertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.作者: COMA 時間: 2025-3-22 23:52
https://doi.org/10.1007/978-3-031-66108-2ve cell imaging with gene knockdown experiments. Here we describe simple methods for microinjecting . eggs with mRNA encoding fluorescent fusion proteins combined with 4D time-lapse imaging techniques we use to follow all of embryonic development from the egg to late tailbud stage.作者: Pandemic 時間: 2025-3-23 03:28
Interest of Non-destructive Tests,escribe expression and purification of GFP/RFP-tagged proteins involved in regulation of DNA replication. We also explain the techniques used to introduce recombinant proteins and fluorescent tubulin into sea urchin eggs and embryos.作者: 摻和 時間: 2025-3-23 09:37 作者: evince 時間: 2025-3-23 12:27 作者: 煤渣 時間: 2025-3-23 15:06 作者: 智力高 時間: 2025-3-23 19:44 作者: 希望 時間: 2025-3-23 23:51
https://doi.org/10.1007/978-3-031-66108-2cue experiments. The simultaneous localization of an in situ RNA hybridization probe and a cell type specific antigen has enabled studies of gene expression in specific types of neurons. Fixatives and antibodies can be capricious; thus, we provide data on preservation of antigens with commonly used fixatives and buffers.作者: Incorruptible 時間: 2025-3-24 04:56
Imaging Neural Development in Embryonic and Larval Sea Urchins,cue experiments. The simultaneous localization of an in situ RNA hybridization probe and a cell type specific antigen has enabled studies of gene expression in specific types of neurons. Fixatives and antibodies can be capricious; thus, we provide data on preservation of antigens with commonly used fixatives and buffers.作者: 品牌 時間: 2025-3-24 10:31
1064-3745 otes and implementation advice from the experts.Includes sup.In .Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, .expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the la作者: expeditious 時間: 2025-3-24 10:54
https://doi.org/10.1007/978-3-476-99751-7 are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial seawater. A protocol is also presented for obtaining electrophysiological recordings of ciliary arrest spikes from intact, behaving larvae, as putative neural correlates of larval settlement.作者: 高貴領(lǐng)導(dǎo) 時間: 2025-3-24 15:35
https://doi.org/10.1007/978-3-476-99751-7o three types, cyphonautes (shelled, feeding), pseudocyphonautes (shelled, nonfeeding), and coronate (unshelled, nonfeeding), although each group is heterogeneous and probably includes various morphologies that are largely undescribed. Here, methods for rearing bryozoan colonies and larvae are presented.作者: Heresy 時間: 2025-3-24 20:49 作者: flutter 時間: 2025-3-25 00:18 作者: LVAD360 時間: 2025-3-25 04:57
Folgen sexualisierter Gewalt im BdP,hods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.作者: 分貝 時間: 2025-3-25 09:41
https://doi.org/10.1007/978-981-97-4414-5mertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.作者: Affection 時間: 2025-3-25 13:10 作者: alcoholism 時間: 2025-3-25 15:54
https://doi.org/10.1007/978-3-658-44702-1 exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.作者: 教唆 時間: 2025-3-25 22:24 作者: BET 時間: 2025-3-26 01:06
Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.作者: exacerbate 時間: 2025-3-26 07:15 作者: 糾纏,纏繞 時間: 2025-3-26 11:30 作者: Perineum 時間: 2025-3-26 15:02
Isolating Specific Embryonic Cells of the Sea Urchin by FACS,, and conditions for FACS of a rare cell type in the embryo. This protocol may be adapted for analysis of mRNA, chromatin, protein, or carbohydrates and depends only on the probe availability for the cell of interest. We anticipate that this protocol will be broadly applicable to embryos of other sp作者: 暴露他抗議 時間: 2025-3-26 19:56 作者: 預(yù)知 時間: 2025-3-26 21:53 作者: 責(zé)怪 時間: 2025-3-27 01:16
Book 2014g known pitfalls..Authoritative and practical, .Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols. seeks to aid scientists in the further study into sea urchins and other marine invertebrates. .作者: 繁榮中國 時間: 2025-3-27 07:52
Goethes Begriff der Weltliteratur especially with batch cultures that lack continuously renewed water. Reverse filtration can be used to change water gently at varying frequencies, depending on temperature and the kinds of food that are provided. Bacterial growth can be limited by antibiotics, but antibiotics are often unnecessary.作者: 牌帶來 時間: 2025-3-27 11:07 作者: cogitate 時間: 2025-3-27 14:40 作者: 健談 時間: 2025-3-27 20:45 作者: 骨 時間: 2025-3-27 22:10
Artificial Seawater Culture of the Gastropod , for Studies of Larval Settlement and Metamorphosis,e (>1 mm) veliger larvae of this species are well suited for neuroethological investigations of settlement and metamorphosis. In this chapter, methods are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial sea作者: 金盤是高原 時間: 2025-3-28 04:16
,, an Enigmatic Basal Metazoan with Potential,cular and behavioral work is essential to better understand the unique and curious life style of these organisms. We provide basic instructions on how . can be cultured and studied in the laboratory emphasizing behavioral and cellular aspects.作者: Blanch 時間: 2025-3-28 06:29 作者: pacific 時間: 2025-3-28 13:48
,An Invertebrate Embryologist’s Guide to Routine Processing of Confocal Images,set of straightforward guidelines will help ensure that image manipulations are both credible and repeatable. Meanwhile, attention to optimal data collection parameters will greatly simplify image processing, not only for convenience but for quality and credibility as well. Here I describe how to co作者: interpose 時間: 2025-3-28 15:18 作者: 性滿足 時間: 2025-3-28 19:51 作者: 禁止 時間: 2025-3-29 00:31 作者: 真實的你 時間: 2025-3-29 03:26 作者: 憤怒歷史 時間: 2025-3-29 10:54
Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian ,,ortical structures like the CAB in ascidians, etc.) combined with targeted gene knockdown during embryonic development is a powerful technique to understand the mechanisms of embryonic development. The eggs and embryos of the primitive marine chordate . are an excellent model system for combining li作者: 支形吊燈 時間: 2025-3-29 13:30 作者: moribund 時間: 2025-3-29 18:57
Biotinylation of Oocyte Cell Surface Proteins of the Starfish , ,rom the cell surface to relevant intracellular compartments. To identify starfish oocyte and egg cell surface proteins, a biotinylation method was adapted from prior protocols using B cells, leukocytes, mouse oocytes, and sea urchin eggs (Cole et al. Mol Immunol 24:699–705, 1987; Flaherty and Swann 作者: Canary 時間: 2025-3-29 23:40 作者: 喊叫 時間: 2025-3-30 01:33 作者: 閃光你我 時間: 2025-3-30 07:26
Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nar extracts. This chapter describes a traditional, film-based immunoblotting method for monitoring the phosphorylation status of proteins in marine nemertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular p作者: 切割 時間: 2025-3-30 10:46
Multicolor Labeling in Developmental Gene Regulatory Network Analysis,lor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for cons作者: probate 時間: 2025-3-30 15:16 作者: MELD 時間: 2025-3-30 17:38 作者: HAIRY 時間: 2025-3-30 21:36 作者: 其他 時間: 2025-3-31 04:55 作者: Leaven 時間: 2025-3-31 05:33 作者: 權(quán)宜之計 時間: 2025-3-31 11:58 作者: 表兩個 時間: 2025-3-31 15:35
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270071.jpg作者: 帶來墨水 時間: 2025-3-31 21:12 作者: Lipoprotein(A) 時間: 2025-3-31 23:06
https://doi.org/10.1007/978-3-476-99751-7e (>1 mm) veliger larvae of this species are well suited for neuroethological investigations of settlement and metamorphosis. In this chapter, methods are described for conditioning adult broodstock, growing microalgal food for larvae, and culturing larvae to metamorphic competence in artificial sea作者: 鉆孔 時間: 2025-4-1 03:24
https://doi.org/10.1007/978-3-476-99751-7cular and behavioral work is essential to better understand the unique and curious life style of these organisms. We provide basic instructions on how . can be cultured and studied in the laboratory emphasizing behavioral and cellular aspects.作者: GRIN 時間: 2025-4-1 10:04 作者: voluble 時間: 2025-4-1 13:11
https://doi.org/10.1007/978-981-97-7035-9set of straightforward guidelines will help ensure that image manipulations are both credible and repeatable. Meanwhile, attention to optimal data collection parameters will greatly simplify image processing, not only for convenience but for quality and credibility as well. Here I describe how to co作者: 游行 時間: 2025-4-1 15:27
Sohyun An,Theresa Alviar-Martintypes in the gonads and embryos of marine invertebrates. Here, I describe methods for preserving and embedding the reproductive organs of marine arrow worms for TEM, including procedures for staining thick sections for light microscopy and thin sections for TEM.作者: BALK 時間: 2025-4-1 21:19 作者: Expiration 時間: 2025-4-2 00:21
https://doi.org/10.1007/978-3-031-66108-2ch. In particular, studies of neural development have been facilitated by methods that enable the accurate identification of specific types of neurons. Here we describe methods that have been successfully employed to study neural development in sea urchin embryos. Altering gene expression in part of作者: anthesis 時間: 2025-4-2 04:48
