標(biāo)題: Titlebook: Deubiquitinases; Methods and Protocol Julie Maupin-Furlow,Mariola J. Edelmann Book 2023 The Editor(s) (if applicable) and The Author(s), un [打印本頁] 作者: Twinge 時間: 2025-3-21 16:23
書目名稱Deubiquitinases影響因子(影響力)
書目名稱Deubiquitinases影響因子(影響力)學(xué)科排名
書目名稱Deubiquitinases網(wǎng)絡(luò)公開度
書目名稱Deubiquitinases網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Deubiquitinases被引頻次
書目名稱Deubiquitinases被引頻次學(xué)科排名
書目名稱Deubiquitinases年度引用
書目名稱Deubiquitinases年度引用學(xué)科排名
書目名稱Deubiquitinases讀者反饋
書目名稱Deubiquitinases讀者反饋學(xué)科排名
作者: RAGE 時間: 2025-3-21 23:49 作者: 放大 時間: 2025-3-22 03:29
High-Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity,talling substrate translocation. With the proteasome as an approved anticancer target, Rpn11 is therefore an attractive point of attack for the development of new inhibitors, which requires robust biochemical assays to measure DUB activity. Here we describe a method for the purification of the Rpn8/作者: mortgage 時間: 2025-3-22 05:35
Bioinformatical Approaches to the Discovery and Classification of Novel Deubiquitinases,an be used to identify high-confidence DUB candidates from pathogen genomes – even in the complete absence of biochemical or functional annotation. This chapter addresses bioinformatical DUB discovery approaches based on a previously published analysis of DUB evolution. The core set of bioinformatic作者: 憤怒歷史 時間: 2025-3-22 09:11 作者: Lignans 時間: 2025-3-22 14:10 作者: Lignans 時間: 2025-3-22 19:47 作者: 把…比做 時間: 2025-3-22 21:43 作者: 眨眼 時間: 2025-3-23 04:00
Francesco Dramis,Giandomenico Fubellitalling substrate translocation. With the proteasome as an approved anticancer target, Rpn11 is therefore an attractive point of attack for the development of new inhibitors, which requires robust biochemical assays to measure DUB activity. Here we describe a method for the purification of the Rpn8/作者: Pillory 時間: 2025-3-23 09:07 作者: 哭得清醒了 時間: 2025-3-23 12:57
https://doi.org/10.1007/978-3-030-21406-7e describe a step-by-step experimental procedure to assess the activity of PLpro in vitro using a suite of activity-based probes (ABPs) and fluorescent substrates and how they can be applied as fast and yet sensitive methods to calculate kinetic parameters.作者: ODIUM 時間: 2025-3-23 15:34
Julie Maupin-Furlow,Mariola J. EdelmannIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 斜谷 時間: 2025-3-23 19:48 作者: Horizon 時間: 2025-3-24 00:17
Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling a probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible).作者: 門窗的側(cè)柱 時間: 2025-3-24 05:22 作者: MUMP 時間: 2025-3-24 10:05
https://doi.org/10.1007/978-1-0716-2803-4DUB inhibitors; Next-generation phage display; poly-Ub chain; therapeutic opportunities v; innate immuni作者: Noisome 時間: 2025-3-24 14:12 作者: Esophagus 時間: 2025-3-24 16:21
High Mountain Hazards in Uttarakhandd catalytic triad rearrangement, where ubiquitin-binding helps the DUB adopt an active conformation for catalysis. The crystal structure of the apo form of such a DUB, when not bound to ubiquitin, reveals an inactive conformation of the catalytic residues, necessitating the structure of the ubiquiti作者: Repetitions 時間: 2025-3-24 19:19 作者: 調(diào)整 時間: 2025-3-25 00:48
Navdeep Agrawal,Jagabandhu Dixitprecise characterization of both enzyme and DUB inhibitor. These probes are compatible with most plate readers allowing for rapid, facile fluorometric analysis of DUB activity. DUB activity can be measured in purified enzyme reactions, in cell lysates, or in intact cells depending upon the choice of作者: 思考才皺眉 時間: 2025-3-25 04:50
https://doi.org/10.1007/978-3-030-04955-3ification, deubiquitinases (DUBs), have been well described for the model organisms. However, the information about DUBs is still largely lacking for the non-model organisms, such as agriculturally relevant animals. To understand the expression of these enzymes in animal tissues, we have used chemic作者: Kidney-Failure 時間: 2025-3-25 10:13
Dogu’a Tembien’s Tropical Mountain ClimateBs). Since DUBs are highly specific toward ubiquitin and exhibit low activity toward shorter peptides, it is challenging to design truly selective chemical tools to investigate one DUB in biological samples. Incorporating amino acids other than canonical LRG at the P4–P2 positions in the Ub improves作者: 雀斑 時間: 2025-3-25 15:34 作者: 灌輸 時間: 2025-3-25 19:21 作者: Exhilarate 時間: 2025-3-25 23:22 作者: 沉默 時間: 2025-3-26 00:44 作者: 榨取 時間: 2025-3-26 06:25
Amit Patel,Andrew Crooks,Naoru Koizumi/Ubl) protein conjugates as substrates for DUB/DUB-like activity assays. Here we outline how to use archaea to synthesize, purify, and assay the activity of DUB-like enzymes with unusual properties, including catalytic activity in hypersaline conditions, organic solvents, and high temperatures. We a作者: averse 時間: 2025-3-26 12:25
https://doi.org/10.1007/978-3-030-21406-7 in viral propagation. PLpro accomplishes this function by processing the viral polyproteins essential for viral replication and removing the small proteins, ubiquitin and ISG15 from the host’s key immune signaling proteins, thereby preventing the host’s innate immune response. Although PLpro from b作者: Irrepressible 時間: 2025-3-26 15:49 作者: 慢慢沖刷 時間: 2025-3-26 18:18 作者: 成績上升 時間: 2025-3-26 22:43 作者: slipped-disk 時間: 2025-3-27 03:46
Kathleen Stewart Hornsby,Kraig Kingtrate hydrolysis by DUBs releases free Ub or polyubiquitin (polyUb) chains. Whereas most quantitative DUB assays depend on fluorescently labeled artificial substrates, employing a sensor able to detect Ub release in real time makes it possible to monitor DUB activity using virtually any Ub conjugate作者: 規(guī)章 時間: 2025-3-27 06:24
Continuous Spatiotemporal Trajectory Joinsterization in vitro has been challenging. Here we describe an in vitro assay to characterize the biochemical activity of full-length Nsp3 isolated from cells. The assay can be used to evaluate Nsp3 inhibitors.作者: paroxysm 時間: 2025-3-27 10:36 作者: Negligible 時間: 2025-3-27 15:37 作者: Moderate 時間: 2025-3-27 21:35
Francesco Dramis,Giandomenico Fubelli probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible).作者: 載貨清單 時間: 2025-3-28 01:45 作者: 禁止,切斷 時間: 2025-3-28 02:59 作者: 公豬 時間: 2025-3-28 06:16
Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs,n the appearance of a fluorescent signal upon DUB-mediated cleavage of the reagent. In this protocol, we describe the use of such an assay to profile the selectivity of TRABID, a member of the OTU family of DUBs.作者: 阻止 時間: 2025-3-28 13:54
Fluorometric Characterization of DUB Activity: From Single-Enzyme Reactions to Live Intact Cells, analysis of DUB activity. DUB activity can be measured in purified enzyme reactions, in cell lysates, or in intact cells depending upon the choice of the fluorometric probe. This chapter describes protocols and potential analysis tools to investigate DUB activity in these three scenarios.作者: REIGN 時間: 2025-3-28 15:10
Identification of Deubiquitinase Substrates in , by Systematic Overexpression, in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in . to overcome these challenges and identify DUB substrates in cells.作者: wangle 時間: 2025-3-28 19:41 作者: 假 時間: 2025-3-29 01:20
Amit Patel,Andrew Crooks,Naoru Koizumilso outline the application of archaea in forming Ub/Ubl isopeptide linkages that include the covalent attachments of diverse archaeal and eukaryotic Ub/Ubls to target proteins. Archaea form these Ub/Ubl-linked protein conjugates in vivo, and the resulting products are found to serve as useful DUB substrates for in vitro assays.作者: CRATE 時間: 2025-3-29 06:25 作者: 污穢 時間: 2025-3-29 10:17 作者: CALL 時間: 2025-3-29 14:29
https://doi.org/10.1007/978-3-030-21406-7e pinpoint DUBs sufficient to counteract proteasomal degradation of these newly identified substrates. We apply this method to . egg extract but suggest that it can also be adapted to other cell lysates.作者: Ataxia 時間: 2025-3-29 17:30 作者: 中國紀(jì)念碑 時間: 2025-3-29 22:41
Identification of Deubiquitinase Substrates in , Egg Extract,e pinpoint DUBs sufficient to counteract proteasomal degradation of these newly identified substrates. We apply this method to . egg extract but suggest that it can also be adapted to other cell lysates.作者: 謊言 時間: 2025-3-30 00:52 作者: Nostalgia 時間: 2025-3-30 04:47 作者: deciduous 時間: 2025-3-30 09:14
Navdeep Agrawal,Jagabandhu Dixit analysis of DUB activity. DUB activity can be measured in purified enzyme reactions, in cell lysates, or in intact cells depending upon the choice of the fluorometric probe. This chapter describes protocols and potential analysis tools to investigate DUB activity in these three scenarios.作者: 子女 時間: 2025-3-30 15:12
Lars Kulik,Egemen Tanin,Muhammad Umer in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in . to overcome these challenges and identify DUB substrates in cells.作者: Inflamed 時間: 2025-3-30 19:54 作者: Bucket 時間: 2025-3-31 00:00 作者: 白楊魚 時間: 2025-3-31 04:01 作者: dominant 時間: 2025-3-31 05:41
Design and Synthesis of Ubiquitin-Based Chemical Tools with Unnatural Amino Acids for Selective Dettorial substrate library (HyCoSuL) approach that can be introduced in the C-terminal motif of Ub. Furthermore, we describe the synthesis protocol of Ub-based probes and substrates containing unnatural amino acids and the application of Ub-based probes to detect DUBs in cell lysates.作者: hypotension 時間: 2025-3-31 10:26 作者: epinephrine 時間: 2025-3-31 16:18
