標(biāo)題: Titlebook: Design and Delivery of SiRNA Therapeutics; Henrik J. Ditzel,Martina Tuttolomondo,Sakari Kaupp Book 2021 Springer Science+Business Media, L [打印本頁] 作者: GURU 時間: 2025-3-21 20:02
書目名稱Design and Delivery of SiRNA Therapeutics影響因子(影響力)
書目名稱Design and Delivery of SiRNA Therapeutics影響因子(影響力)學(xué)科排名
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書目名稱Design and Delivery of SiRNA Therapeutics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Design and Delivery of SiRNA Therapeutics被引頻次
書目名稱Design and Delivery of SiRNA Therapeutics被引頻次學(xué)科排名
書目名稱Design and Delivery of SiRNA Therapeutics年度引用
書目名稱Design and Delivery of SiRNA Therapeutics年度引用學(xué)科排名
書目名稱Design and Delivery of SiRNA Therapeutics讀者反饋
書目名稱Design and Delivery of SiRNA Therapeutics讀者反饋學(xué)科排名
作者: 粗糙濫制 時間: 2025-3-21 23:02 作者: kidney 時間: 2025-3-22 01:42
,Engineering of Solid Dosage Forms of siRNA-Loaded Lipidoid–Polymer Hybrid Nanoparticles Using a Qua-glycolic acid) to effectively deliver siRNA directed against tumor necrosis factor alpha (TNF-α) intracellularly to macrophages. L. is a novel lipid-like material consisting of a tetraamine backbone linked to five C. alkyl chains. Here, we describe a systematic quality-by-design (QbD) approach incl作者: Coronary 時間: 2025-3-22 08:22
Cell-Penetrating Peptides Delivering siRNAs: An Overview,thesis, use, and versatility. Recent progress with siRNA delivered by CPPs seems to focus on targeted delivery to reduce side effects and amount of drugs used, and it appears to be among the most promising use for CPPs in future clinical applications.作者: Generic-Drug 時間: 2025-3-22 10:15 作者: 盤旋 時間: 2025-3-22 14:43
siRNA Vehicles for High Endosomal Escapability, a highly required characteristic for the induction of a high knockdown effect. Here, we describe the step-by-step procedure for the evaluation of high endosomal escapability. The vector that has pH-responsive characteristics at around pH?=?6.2–6.5 is important for the high endosomal escape.作者: 盤旋 時間: 2025-3-22 17:18
siRNA Design and Delivery Based on Carbon Nanotubes,he past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.作者: Felicitous 時間: 2025-3-22 21:51 作者: 是突襲 時間: 2025-3-23 02:12 作者: menopause 時間: 2025-3-23 07:22 作者: 提煉 時間: 2025-3-23 12:14
Book 2021 to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,?.Design and Delivery of SiRNA Therapeutics .aims to ensure successful results in the further study of this vital field..作者: 性滿足 時間: 2025-3-23 14:37 作者: FLORA 時間: 2025-3-23 19:54
,Gew?hnliche Differentialgleichungen,ivo gene silencing effect and reduce the toxicity. Here we describe the preparation of LPH and LCP NPs loaded with siRNA, and characterization analysis including size distribution, trapping efficiency, and in vivo activity. This protocol could be used for in vivo delivery of siRNA to target genes in cancer cells.作者: Adenocarcinoma 時間: 2025-3-24 01:19
Book 2021o an effective administration?.in vivo.. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troub作者: meritorious 時間: 2025-3-24 05:38 作者: indicate 時間: 2025-3-24 08:38 作者: Discrete 時間: 2025-3-24 11:00
1064-3745 ation advice from the experts.This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration?.in vivo.. Written in the highly successful .Methods in Molecular Biology .series format, c作者: 江湖騙子 時間: 2025-3-24 17:31
Extremwertaufgaben und Optimierung,methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast F?rster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.作者: HERTZ 時間: 2025-3-24 22:06
Abbildungen, reelle Funktionen, a highly required characteristic for the induction of a high knockdown effect. Here, we describe the step-by-step procedure for the evaluation of high endosomal escapability. The vector that has pH-responsive characteristics at around pH?=?6.2–6.5 is important for the high endosomal escape.作者: Fecal-Impaction 時間: 2025-3-24 23:33
Formeln und Fakten im Grundkurs Mathematikhe past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.作者: ALIEN 時間: 2025-3-25 04:18 作者: Limousine 時間: 2025-3-25 10:58
Formeln und Tabellen Grundwissen Technik obtained with any other available experimental technique. In this chapter we describe the main computational toolbox that can be exploited in the different stages of novel dendritic nanocarrier production—from the initial conception to the stage of biological intermolecular interactions.作者: congenial 時間: 2025-3-25 11:43 作者: 鋼筆記下懲罰 時間: 2025-3-25 17:42
1064-3745 protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,?.Design and Delivery of SiRNA Therapeutics .aims to ensure successful results in the further study of this vital field..978-1-0716-1300-9978-1-0716-1298-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 相同 時間: 2025-3-25 21:52 作者: regale 時間: 2025-3-26 04:01 作者: 惡臭 時間: 2025-3-26 05:20
Extremwertaufgaben und Optimierung,iption of genes, target mRNA for site-specific cleavage, or block mRNA translation into proteins. This review outlines the history of RNAi discovery, function, and mechanisms of action. For comparison, it also touches on CRISPR interference.作者: Tidious 時間: 2025-3-26 12:23
https://doi.org/10.1007/978-3-322-94869-4tamer, and the antisense strand through “stick” complementary sequences elongated at their 3′ end, and the subsequent paring with the sense strand. Such a protocol allows a modular non-covalent generation of the constructs and permits an efficient delivery of the siRNA moiety into aptamer target cells.作者: 臨時抱佛腳 時間: 2025-3-26 16:32
https://doi.org/10.1007/978-3-322-94869-4nd givosiran for the treatment of acute hepatic porphyria, respectively. Here, we describe the current strategies for delivering siRNA drugs to the liver and summarize recent advances in clinical development of siRNA therapeutics for the treatment of liver diseases.作者: Pcos971 時間: 2025-3-26 20:20
Abbildungen, reelle Funktionen,lity and reduce its off-target effect. Also, the delivery method based on .-acetylgalactosamine (GalNAc)-siRNA conjugate that is widely employed to develop therapeutic regimens for liver-related diseases is also recapitulated.作者: forebear 時間: 2025-3-26 23:18
Abbildungen, reelle Funktionen,itro and in vivo. Here we provide a robust protocol for the synthesis of triple GalNAc CPG solid support and GalNAc phosphoramidite, synthesis and purification of RNA conjugates with multiple GalNAc residues either to 5′-end or 3′-end and siRNA duplex formation.作者: 笨拙的我 時間: 2025-3-27 01:19
Wilfried Pla?mann,Detlef Schulz stability of the siRNA/NV ensemble; (3) characterization and quantification of the cellular uptake and release of the siRNA fragment; (4) in vitro and (5) in vivo experiments for the evaluation of the corresponding gene silencing activity; and (6) assessment of the intrinsic toxicity of the NV and the siRNA/NV complex.作者: Adulate 時間: 2025-3-27 06:02 作者: 歡笑 時間: 2025-3-27 11:19 作者: overrule 時間: 2025-3-27 15:39 作者: 失眠癥 時間: 2025-3-27 20:46 作者: aesthetician 時間: 2025-3-28 00:34
Development of siRNA Therapeutics for the Treatment of Liver Diseases,nd givosiran for the treatment of acute hepatic porphyria, respectively. Here, we describe the current strategies for delivering siRNA drugs to the liver and summarize recent advances in clinical development of siRNA therapeutics for the treatment of liver diseases.作者: 發(fā)生 時間: 2025-3-28 05:45
siRNA Design and GalNAc-Empowered Hepatic Targeted Delivery,lity and reduce its off-target effect. Also, the delivery method based on .-acetylgalactosamine (GalNAc)-siRNA conjugate that is widely employed to develop therapeutic regimens for liver-related diseases is also recapitulated.作者: 手銬 時間: 2025-3-28 06:48 作者: FORGO 時間: 2025-3-28 13:56 作者: 不足的東西 時間: 2025-3-28 17:40
ITC for Characterization of Self-Assembly Process of Cationic Dendrons for siRNA Delivery, or via specific analysis methods, a full thermodynamic characterization of these nanomicelles, including their critical micellar concentration, micelle aggregation number, degree of counterion binding, Gibbs free energy of micellization, and its enthalpic and entropic components.作者: Hypomania 時間: 2025-3-28 20:57 作者: 兩棲動物 時間: 2025-3-29 01:26 作者: ENACT 時間: 2025-3-29 03:29
https://doi.org/10.1007/978-3-322-94869-4 with perfect sequence complementarity. However, siRNA often exhibits off-target effects on genes with partial sequence complementarities. Such off-target effect is an undesirable adverse effect for knocking down a target gene specifically. Here we describe the powerful strategy to avoid off-target 作者: sinoatrial-node 時間: 2025-3-29 10:50
https://doi.org/10.1007/978-3-322-94869-4ed organs and tissues is still a critical need for their translation to the clinic. Here we describe how nucleic acid-based aptamers against cell surface epitopes may be used to address this issue. We discuss the most recent examples and advances in the field of aptamer siRNA delivery and provide a 作者: 犬儒主義者 時間: 2025-3-29 11:36
Extremwertaufgaben und Optimierung,recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast F?rster resonance ene作者: Gratuitous 時間: 2025-3-29 17:20 作者: fatty-acids 時間: 2025-3-29 20:49
Abbildungen, reelle Funktionen, also from pharmaceutical industry. As siRNA can theoretically modulate any disease-related gene’s expression, plenty of siRNA therapeutic pipelines have been established by tens of biotechnology companies. The drug performance of siRNA heavily depends on the sequence, the chemical modification, and作者: Concrete 時間: 2025-3-29 23:58 作者: Ascribe 時間: 2025-3-30 06:38
Formeln und Fakten im Grundkurs Mathematikymers, antibodies, and aptamers either at the 3′- or 5′-termini of a siRNA duplex molecule has resulted in a plethora of siRNA bioconjugates with improved stabilities in bloodstream and better pharmacokinetic values than unmodified siRNAs. In this sense, lipid-siRNA conjugates have attracted a remar作者: 討好美人 時間: 2025-3-30 11:57 作者: 屈尊 時間: 2025-3-30 14:48
,Gew?hnliche Differentialgleichungen,ived nanoparticles (NPs) are one of the best investigated systems for in vivo siRNA delivery. In the recent years, we have successfully redesigned the conventional cationic liposomes into Liposome/Protamine/hyaluronic acid (LPH) NPs and Lipid-Calcium-Phosphate (LCP) NPs in order to increase the in v作者: stress-response 時間: 2025-3-30 17:48
Abbildungen, reelle Funktionen,livery vector is imperative for clinical use. Since siRNA works in the cytoplasm, the ability of the carrier to escape destruction in the endosomes is a highly required characteristic for the induction of a high knockdown effect. Here, we describe the step-by-step procedure for the evaluation of hig作者: Omnipotent 時間: 2025-3-30 22:04 作者: 最初 時間: 2025-3-31 01:23 作者: DAFT 時間: 2025-3-31 07:06
Wilfried Pla?mann,Detlef Schulzcovalent cationic dendrimers (CCDs) and self-assembled cationic dendrons (ACDs) for siRNA delivery in the presence and absence of their nucleic cargos. On the basis of the reported examples, a standard essential set of techniques is described for each step of a siRNA/nanovector (NV) complex characte作者: MURAL 時間: 2025-3-31 10:13 作者: 外面 時間: 2025-3-31 13:33 作者: 仲裁者 時間: 2025-3-31 19:24 作者: apropos 時間: 2025-4-1 01:17
https://doi.org/10.1007/978-3-322-89910-1g RNAs (siRNA) are double-stranded RNA molecules capable of silencing the expression of a specific protein triggering the RNA interference (RNAi) pathway, but they are unable to cross the plasma membrane and have a short half-life in the bloodstream. In this overview, we assessed the many different 作者: TOM 時間: 2025-4-1 03:37
Formeln und Tabellen Grundwissen Technikating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA enc作者: Cabinet 時間: 2025-4-1 07:20
