作者: 做作 時(shí)間: 2025-3-21 20:49 作者: 吝嗇性 時(shí)間: 2025-3-22 01:21
Christoph Fuchs,Franziska J. Golenhofenke tyrosine kinase 3 ligand (Flt3L), either directly through a series of subcutaneous injections or by seeding with an Flt3L secreting murine melanoma. Flt3L may also be added to bone marrow cultures to produce large numbers of in vitro equivalents of the spleen DC subsets. Total DC, or their subset作者: 變化 時(shí)間: 2025-3-22 08:02 作者: 大酒杯 時(shí)間: 2025-3-22 12:41 作者: Pastry 時(shí)間: 2025-3-22 16:17 作者: Pastry 時(shí)間: 2025-3-22 20:53 作者: Lacerate 時(shí)間: 2025-3-22 23:37
Isolation of Human Skin Dendritic Cell Subsets7–2596, 1993; Nestle et al., J Immunol 151:6535–6545, 1993). These factors led to the extensive use of skin DCs as the “prototype” migratory DCs in human studies. In this chapter, we detail the protocols to isolate DCs and resident macrophages from human skin. We also provide a multiparameter flow c作者: ascetic 時(shí)間: 2025-3-23 05:10 作者: 鞭子 時(shí)間: 2025-3-23 07:45 作者: Aggressive 時(shí)間: 2025-3-23 09:48 作者: 時(shí)代錯(cuò)誤 時(shí)間: 2025-3-23 17:26
Cross-Presentation Assay for Human Dendritic Cellsitiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.作者: 不成比例 時(shí)間: 2025-3-23 18:05 作者: 決定性 時(shí)間: 2025-3-24 02:01 作者: falsehood 時(shí)間: 2025-3-24 03:39 作者: 鉗子 時(shí)間: 2025-3-24 09:17 作者: PALSY 時(shí)間: 2025-3-24 11:19 作者: 權(quán)宜之計(jì) 時(shí)間: 2025-3-24 18:00 作者: 全國(guó)性 時(shí)間: 2025-3-24 19:48 作者: 乞丐 時(shí)間: 2025-3-25 00:19
Andreas Oehler,Matthias Horn,Stefan WendtThe efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.作者: Hirsutism 時(shí)間: 2025-3-25 04:05 作者: 相符 時(shí)間: 2025-3-25 10:33
Katja Schechtner,Melinda Hansont dendritic cells are heterogeneous and comprise several subsets that can be distinguished by their surface phenotype, ontogeny, and molecular signature. This review gives an overview of mouse and human dendritic cell subsets and their defining features and summarizes the current knowledge of dendri作者: ASSAY 時(shí)間: 2025-3-25 12:27
Does Your Idea Have Disruptive Potential?Most of the information gained on human DC biology was derived from studies conducted with DCs generated in vitro from peripheral blood CD14. monocytes (MoDCs) or from CD34. hematopoietic progenitors. Recent advances in the field revealed that these types of in vitro-derived DCs strikingly differ fr作者: NICHE 時(shí)間: 2025-3-25 18:29 作者: 多余 時(shí)間: 2025-3-25 21:23 作者: 錯(cuò)事 時(shí)間: 2025-3-26 01:07 作者: 結(jié)果 時(shí)間: 2025-3-26 04:24
Modularisierung und Plattformenr understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.作者: 聽寫 時(shí)間: 2025-3-26 10:27
Christoph Fuchs,Franziska J. Golenhofenls from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small a作者: Visual-Acuity 時(shí)間: 2025-3-26 15:47
Christoph Fuchs,Franziska J. Golenhofen anatomical location, phenotype and function. In human, the two most accessible tissues to study leukocytes are peripheral blood and skin. DCs are rare in human peripheral blood (<1?% of mononuclear cells) and have a less mature phenotype than their tissue counterparts (MacDonald et al., Blood. 100:作者: figurine 時(shí)間: 2025-3-26 18:19 作者: 啟發(fā) 時(shí)間: 2025-3-26 23:54 作者: confederacy 時(shí)間: 2025-3-27 01:10
Heinrich Holland,Nandhini Ramanathan now, from peripheral blood: plasmacytoid dendritic cells (pDCs), CD141. myeloid DCs, and CD1c. myeloid DCs. The method is based on the sequential depletion of non-DCs. First, depletion of granulocytes, erythrocytes, and platelets is obtained by blood centrifugation over a Ficoll gradient. Then, ant作者: CLAM 時(shí)間: 2025-3-27 06:54 作者: 消瘦 時(shí)間: 2025-3-27 12:11 作者: 玩笑 時(shí)間: 2025-3-27 16:07
System Reliability: A Cold War Lessonf receptor depends on different factors, including their capacity to induce internalization of the delivered antigen/adjuvant cargo. Assays currently used to monitor internalization in dendritic cells have several limitations. We have developed a novel DNA-based probe that allows for simple and robu作者: Excitotoxin 時(shí)間: 2025-3-27 19:19
Book 2016Latest editionitic cells(DC). An introductoryreview provides an overview of recent advances in the characterization of DCsubsets in mouse and human. While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell p作者: 一起 時(shí)間: 2025-3-28 00:40 作者: Neuropeptides 時(shí)間: 2025-3-28 05:37
Katja Schechtner,Melinda Hansonre. This review gives an overview of mouse and human dendritic cell subsets and their defining features and summarizes the current knowledge of dendritic cell subsets’ functional specialization in terms of antigen presentation.作者: 教唆 時(shí)間: 2025-3-28 06:35 作者: 煩擾 時(shí)間: 2025-3-28 14:10 作者: CHAR 時(shí)間: 2025-3-28 16:41 作者: staging 時(shí)間: 2025-3-28 22:50
Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Mear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.作者: 破譯密碼 時(shí)間: 2025-3-29 01:16
Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skinlogy upon infection. Here, we describe the procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.作者: 可商量 時(shí)間: 2025-3-29 05:44 作者: maudtin 時(shí)間: 2025-3-29 09:19 作者: 整理 時(shí)間: 2025-3-29 14:46 作者: 怎樣才咆哮 時(shí)間: 2025-3-29 18:31
Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assaytive cytosolic substrate of β-lactamase, CCF4. Following uptake of β-lactamase by the DCs, the enzyme undergoes export to the cytosol leading to cleavage of the FRET dye. This cleavage and switch of fluorescence are analyzed by flow cytometry, allowing a quantitative measurement of this event.作者: Verify 時(shí)間: 2025-3-29 20:09 作者: 熱心 時(shí)間: 2025-3-30 00:54
Heinrich Holland,Nandhini Ramanathanmarkers..DC studies might contribute to the comprehension of human immune processes in physiological and pathological conditions. Human blood DCs targeting might be a useful tool to ameliorate inflammatory diseases and improve vaccination strategies.作者: 競(jìng)選運(yùn)動(dòng) 時(shí)間: 2025-3-30 04:48 作者: 不理會(huì) 時(shí)間: 2025-3-30 11:01 作者: 善于 時(shí)間: 2025-3-30 14:04 作者: Ccu106 時(shí)間: 2025-3-30 20:00
Analysis of Intracellular Trafficking of Dendritic Cell Receptors for Antigen Targetinguish internalized from surface-bound material. Here, we describe the steps for modifying material (antibodies, proteins) with the probe, undertaking the assay, and analyzing the data obtained from flow cytometry.作者: neologism 時(shí)間: 2025-3-31 00:43
Review of Mouse and Human Dendritic Cell Subsetst dendritic cells are heterogeneous and comprise several subsets that can be distinguished by their surface phenotype, ontogeny, and molecular signature. This review gives an overview of mouse and human dendritic cell subsets and their defining features and summarizes the current knowledge of dendri作者: 氣候 時(shí)間: 2025-3-31 02:40 作者: 攀登 時(shí)間: 2025-3-31 08:11 作者: constitutional 時(shí)間: 2025-3-31 13:12
Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse a short half-life and therefore, require continuous generation from hematopoietic stem cells and progenitor cells. Recently, we identified DC-restricted progenitors called common DC progenitors (CDPs) in the bone marrow of mouse. The CDPs can be isolated from mouse bone marrow based on the hematopo
