標(biāo)題: Titlebook: DNA-Protein Interactions; Principles and Proto Beno?t P. Leblanc,Sébastien Rodrigue Book 2015Latest edition Springer Science+Business Media [打印本頁] 作者: 不正常 時(shí)間: 2025-3-21 18:53
書目名稱DNA-Protein Interactions影響因子(影響力)
作者: enterprise 時(shí)間: 2025-3-21 20:14 作者: lipoatrophy 時(shí)間: 2025-3-22 03:10
Single-Molecule Approaches for the Characterization of Riboswitch Folding Mechanisms,l changes, thus enabling to deduce complex riboswitch folding pathways. Herein, we show how to employ sm-FRET to study the folding pathway of the .-adenosylmethionine (SAM) and how this can be used to understand very specific conformational changes that are at the heart of riboswitch regulation mech作者: AMITY 時(shí)間: 2025-3-22 05:50 作者: 歹徒 時(shí)間: 2025-3-22 09:24 作者: Axillary 時(shí)間: 2025-3-22 14:22 作者: Axillary 時(shí)間: 2025-3-22 17:21
B. Scherrer,M. Dojat,F. Forbes,C. Garbayhese analyses were combined with 3-D models of the available structures of portions of the activator protein and B-form DNA to obtain a 3-D picture of the protein relative to the DNA. Finally, the Molsoft program was used to refine the position, revealing the architecture of the protein–DNA within t作者: emission 時(shí)間: 2025-3-22 23:01 作者: 一瞥 時(shí)間: 2025-3-23 03:19 作者: nostrum 時(shí)間: 2025-3-23 07:09 作者: BRIDE 時(shí)間: 2025-3-23 12:38
Precise Identification of DNA-Binding Proteins Genomic Location by Exonuclease Coupled Chromatin Im作者: FUME 時(shí)間: 2025-3-23 14:39 作者: Bridle 時(shí)間: 2025-3-23 21:40
DNA-Protein Interactions978-1-4939-2877-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: GORGE 時(shí)間: 2025-3-23 23:42 作者: 手榴彈 時(shí)間: 2025-3-24 03:10 作者: 收集 時(shí)間: 2025-3-24 08:28
978-1-4939-4945-8Springer Science+Business Media New York 2015作者: inflame 時(shí)間: 2025-3-24 13:08 作者: indubitable 時(shí)間: 2025-3-24 15:06 作者: folliculitis 時(shí)間: 2025-3-24 20:39 作者: 比賽用背帶 時(shí)間: 2025-3-25 01:43 作者: 協(xié)奏曲 時(shí)間: 2025-3-25 05:58
Lecture Notes in Computer Scienceusing bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments generated by the partial digestion of a DNA sequence in the absence of a protein to that pro作者: habile 時(shí)間: 2025-3-25 10:02 作者: Lipoma 時(shí)間: 2025-3-25 15:22 作者: airborne 時(shí)間: 2025-3-25 18:19
Mar Marcos,Jose M. Juarez,Gregor Stiglicled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electropho作者: Coronation 時(shí)間: 2025-3-25 23:36 作者: 倒轉(zhuǎn) 時(shí)間: 2025-3-26 01:50 作者: 可以任性 時(shí)間: 2025-3-26 07:18
Olga Dolinina,Igor Bessmertny,Vadim Zhmud and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to p作者: interior 時(shí)間: 2025-3-26 08:57 作者: 顯而易見 時(shí)間: 2025-3-26 13:58 作者: LASH 時(shí)間: 2025-3-26 17:36
https://doi.org/10.1007/978-3-031-06242-1y only a weak sequence preference, making the analysis of their DNA-binding characteristics difficult if not impossible in a standard electrophoretic mobility shift assay (EMSA). In contrast, such proteins often bind prebent DNAs with high affinity and specificity. A synthetic cruciform DNA structur作者: 決定性 時(shí)間: 2025-3-27 01:01
Georgios I. Doukidis,Ray J. Paulrder to regulate gene expression making it relevant to determine the profiles of cohabitation of several proteins on the chromatin fiber. Chromatin immunoprecipitation (ChIP) has been broadly used to determine the profile of several histone posttranslational modifications as well as transcription fa作者: impaction 時(shí)間: 2025-3-27 01:37
Georgios I. Doukidis,Ray J. Pauluence-programmable tools for various purposes such as genome editing and transcriptional regulation. A critical aspect of the system is the selection and validation of spacer sequences that allow precise targeting of the guide RNA-Cas9 complex. We describe a procedure involving computational and exp作者: gonioscopy 時(shí)間: 2025-3-27 07:32 作者: EXALT 時(shí)間: 2025-3-27 11:50
Studies in Computational Intelligencetranscriptional processes in that alteration of the interaction between its components results in the deregulation of cellular transcriptional program. Modification of epigenetic marks, variation in the precise positioning of nucleosomes, and consequent mobilization of nucleosomes regulate the acces作者: 描繪 時(shí)間: 2025-3-27 14:38
https://doi.org/10.1007/978-3-030-97269-1 it is often useful to represent the signal over known regions of interest, such as genes, using aggregate or individual profiles. In the present chapter, we describe and explain the best practices on how to generate such profiles as well as other usages of the versatile aggregate profiler (VAP) too作者: 地名表 時(shí)間: 2025-3-27 19:50 作者: Horizon 時(shí)間: 2025-3-28 01:38
Electrophoretic Mobility Shift Assay Using Radiolabeled DNA Probes,col, a purified protein of interest is mixed with a 5′-end radiolabeled DNA probe. The bound complexes are separated by electrophoretic migration through a polyacrylamide gel and detected with a phosphorimager. The applications of EMSA are diverse, from thermodynamic and kinetic analyses to observat作者: 新陳代謝 時(shí)間: 2025-3-28 03:32
In Vitro DNase I Footprinting,using bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments generated by the partial digestion of a DNA sequence in the absence of a protein to that pro作者: CLAN 時(shí)間: 2025-3-28 08:05
,Determining the Architecture of a Protein–DNA Complex by Combining FeBABE Cleavage Analyses, 3-D Prologous proteins from which the DNA binding can be inferred, and/or if only portions of the protein can be crystallized. If the protein comprises just a part of a large multi-subunit complex, other complications can arise such as the complex being too large for NMR studies, or it is not possible to 作者: 讓步 時(shí)間: 2025-3-28 10:53
In Cellulo DNA Analysis: LMPCR Footprinting,lation of gene expression. The nuclease-hypersensitive sites and sequences bound by transcription factors often correspond to genetic regulatory elements. Using the ligation-mediated polymerase chain reaction (LMPCR) technology, it is possible to precisely analyze these DNA sequences to demonstrate 作者: TRACE 時(shí)間: 2025-3-28 15:37 作者: Omniscient 時(shí)間: 2025-3-28 20:50 作者: 狗舍 時(shí)間: 2025-3-28 23:07
Probing of Nascent Riboswitch Transcripts,lecules that are involved in the biosynthesis and transport of cellular metabolites. Because riboswitches regulate gene expression by modulating their structure, it is vital to employ native probing assays to determine how native riboswitch structures perform highly efficient and specific ligand rec作者: 厭食癥 時(shí)間: 2025-3-29 04:48
Functional Studies of DNA-Protein Interactions Using FRET Techniques, and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to p作者: 做作 時(shí)間: 2025-3-29 08:44
,Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5′-Rapid Amplificatirough 5′-rapid amplification of cDNA ends (5′-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in micr作者: 易彎曲 時(shí)間: 2025-3-29 12:45 作者: 埋葬 時(shí)間: 2025-3-29 18:10 作者: 貨物 時(shí)間: 2025-3-29 20:31 作者: DALLY 時(shí)間: 2025-3-30 03:17 作者: 藥物 時(shí)間: 2025-3-30 04:27
Detection of Short-Range DNA Interactions in Mammalian Cells Using High-Resolution Circular Chromoscial to understanding the molecular mechanisms that regulate gene expression. In this chapter, we present a protocol for high-resolution circular chromosome conformation capture coupled to deep sequencing. This methodology allows to investigate short-range DNA interactions (<100 kbp) and to obtain h作者: 蕨類 時(shí)間: 2025-3-30 08:14 作者: 易發(fā)怒 時(shí)間: 2025-3-30 14:01
Aggregate and Heatmap Representations of Genome-Wide Localization Data Using VAP, a Versatile Aggre it is often useful to represent the signal over known regions of interest, such as genes, using aggregate or individual profiles. In the present chapter, we describe and explain the best practices on how to generate such profiles as well as other usages of the versatile aggregate profiler (VAP) too作者: ureter 時(shí)間: 2025-3-30 18:12
,Circular Dichroism for the Analysis of Protein–DNA Interactions,CD) in protein–nucleic acids interaction solution studies. The chapter will describe the guidelines appropriate to designing experiments and conducting correct data interpretation, the use of both benchtop and synchrotron CD approaches is discussed and the advantages of SRCD outlined. Further inform作者: STALE 時(shí)間: 2025-3-30 22:37 作者: collagen 時(shí)間: 2025-3-31 02:27 作者: Anthology 時(shí)間: 2025-3-31 08:20
Studies in Computational Intelligencee technique entitled FAIRE (formaldehyde-assisted isolation of regulatory elements). Combined with high-throughput sequencing (FAIRE-seq), this procedure allows isolation of nucleosome-free regions and their mapping along the genome, thereby providing a global view of cell-specific regulatory elements.作者: antidote 時(shí)間: 2025-3-31 13:01 作者: aerobic 時(shí)間: 2025-3-31 14:46 作者: Prophylaxis 時(shí)間: 2025-3-31 18:06 作者: 整潔 時(shí)間: 2025-3-31 22:48
Book 2015Latest editionques proven by their continuous value, the volume also many new chapters have been added that mostly deal with larger-scale experiments, reflecting recent advances in "big biology", combining to offer a very useful compendium of protocols allowing readers to delve into the intricacies of protein-DNA作者: STALL 時(shí)間: 2025-4-1 02:09 作者: 遺留之物 時(shí)間: 2025-4-1 07:02
Probing of Nascent Riboswitch Transcripts,ognition. By employing RNase H probing, it is possible to determine the accessibility of specific RNA domains in various structural contexts. Herein, we describe how to employ RNase H probing to characterize nascent mRNA riboswitch molecules as a way to obtain information regarding the riboswitch regulation control mechanism.作者: 掃興 時(shí)間: 2025-4-1 13:35
