標題: Titlebook: DNA-Protein Interactions; Principles and Proto Beno?t Leblanc,Tom Moss Book 2009Latest edition Humana Press 2009 Chromatin immunoprecipitat [打印本頁] 作者: 口語 時間: 2025-3-21 19:51
書目名稱DNA-Protein Interactions影響因子(影響力)
書目名稱DNA-Protein Interactions影響因子(影響力)學科排名
書目名稱DNA-Protein Interactions網絡公開度
書目名稱DNA-Protein Interactions網絡公開度學科排名
書目名稱DNA-Protein Interactions被引頻次
書目名稱DNA-Protein Interactions被引頻次學科排名
書目名稱DNA-Protein Interactions年度引用
書目名稱DNA-Protein Interactions年度引用學科排名
書目名稱DNA-Protein Interactions讀者反饋
書目名稱DNA-Protein Interactions讀者反饋學科排名
作者: 原諒 時間: 2025-3-21 20:51 作者: Audiometry 時間: 2025-3-22 02:44
DNase I Footprinting,using bulky nucleases such as DNAse I. The DNAse I footprinting method was developed to make use of this phenomenon in the study of DNA–protein interactions; it consists in comparing the pattern of fragments produced by the partial digestion of DNA in the absence of a protein to that produced by par作者: palpitate 時間: 2025-3-22 08:01
Exonuclease III Footprinting on Immobilized DNA Templates, protects it from degradation by an enzyme or chemical reagent..Exonuclease III is a suitable probe to analyze the boundaries of a protein when it is necessary to eliminate any excess unbound DNA from the reaction to avoid background problems. In combination with biotin-labeled DNA that is bound to 作者: Neolithic 時間: 2025-3-22 08:59
Hydroxyl Radical Footprinting of Protein-DNA Complexes,cts between a protein and its cognate DNA and details of the complex structure. We describe several methods to prepare DNA templates for footprinting and ways to avoid many of the pitfalls associated with the use of hydroxyl radical footprinting. In addition, we describe in detail one example of the作者: Flounder 時間: 2025-3-22 14:08 作者: Flounder 時間: 2025-3-22 17:40
Uranyl Photofootprinting,elength ultraviolet light (. = 300–420 nm), uranyl ions bound to backbone phosphates oxidize proximal sugars and induce nucleic acid backbone cleavage. Thus the uranyl(VI) ion functions as a very specific and efficient photochemical probe for identifying ligand(protein)-phosphate contacts in nucleic作者: Incorporate 時間: 2025-3-22 22:58
Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylatiol probes, such as dimethyl sulfate, have been used to obtain information on these sites of interaction. Protection and interference patterns frequently correspond to highly conserved positions within binding sites and are often specific for a given transcription factor or family of factors. The meth作者: 來這真柔軟 時間: 2025-3-23 03:13 作者: 高射炮 時間: 2025-3-23 08:15
Identification of Nucleic Acid High-Affinity Binding Sequences of Proteins by SELEX,rtitioning assay. Here, a histidine-tagged protein is allowed to interact with a pool of nucleic acids and the protein–nucleic acid complexes formed are retained on a Ni-NTA matrix. Nucleic acids with a low level of recognition by the protein are washed away. The pool of recovered nucleic acids is a作者: tenuous 時間: 2025-3-23 10:07 作者: 健談的人 時間: 2025-3-23 14:05 作者: 放大 時間: 2025-3-23 22:06 作者: 歌唱隊 時間: 2025-3-24 01:11 作者: Highbrow 時間: 2025-3-24 03:37 作者: Malaise 時間: 2025-3-24 09:58 作者: COKE 時間: 2025-3-24 13:26 作者: 啞劇 時間: 2025-3-24 15:36 作者: murmur 時間: 2025-3-24 21:26 作者: 健談的人 時間: 2025-3-25 00:45
How to?Explain It to?System Testers?r footprinting methods, these reagents can detect such distortions even within the regions of protein–DNA complexes normally protected in other footprinting techniques. Further, reactions are very robust, so that distorted regions can be detected even under conditions where efficiency of DNA–protein作者: abstemious 時間: 2025-3-25 07:15 作者: Congestion 時間: 2025-3-25 10:56 作者: PAN 時間: 2025-3-25 11:40
Steve Williams,J. Mark Ware,Berndt Mülleremical methods, termed chemical footprinting, aim to determine the functional groups on DNA which are protected in solution by bound protein against modification or where chemical pre-modification interferes with subsequent protein binding. One of these approaches, termed ethylation interference foo作者: Intruder 時間: 2025-3-25 18:58 作者: Debate 時間: 2025-3-25 23:08 作者: cluster 時間: 2025-3-26 01:42
Bilash Dash,Tianhua Chen,Richard HillThe most widely used footprinting techniques employ reagents such as deoxyribonuclease I (DNase I) and dimethyl sulfate (DMS) for protection analysis in solution. Nevertheless, these techniques have several disadvantages, and although these may be bypassed by coupling the footprinting reaction with 作者: 全部 時間: 2025-3-26 04:42 作者: RADE 時間: 2025-3-26 11:28
https://doi.org/10.1007/978-94-011-0305-3 by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation. Transcription of protein-encoding genes in mammalian cells is performed by the concerted action of the RNA polymerase II holoenzyme, transcription factors, co-activator complexes that bi作者: Synovial-Fluid 時間: 2025-3-26 15:24
Beno?t Leblanc,Tom MossCovers cutting edge techniques in chromatin studies, such as Nucleosome Mapping, Chromatin Immunoprecipitation and ChIP-on-chip.Contains many protocols for large scale analysis of genome-protein inter作者: 喪失 時間: 2025-3-26 18:48 作者: 真實的你 時間: 2025-3-26 21:20 作者: 得意人 時間: 2025-3-27 02:42 作者: 一再煩擾 時間: 2025-3-27 07:11
https://doi.org/10.1007/978-1-60327-015-1Chromatin immunoprecipitation; DNA; Genome-wide techniques; In vivo techniques; Microarray; PCR; Photocros作者: 江湖郎中 時間: 2025-3-27 09:29 作者: micronized 時間: 2025-3-27 17:21
Samuel Romine,Joshua Jensen,Robert Ball the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.作者: Kernel 時間: 2025-3-27 20:24 作者: 窗簾等 時間: 2025-3-28 01:29 作者: 輕信 時間: 2025-3-28 04:12
Karina Macakaite,Arjab Singh Khumanand detected by their ability to bind radiolabeled DNA. The protein(s) interacting with the labeled DNA is visualized by autoradiography. This technique was used in our laboratory to visualize the metal regulatory consensus sequence-binding protein MTF-1 in L cell crude nuclear extracts.作者: Statins 時間: 2025-3-28 07:44
Book 2009Latest editiongenic crops, the difference between survival and starvation. In .DNA-Protein Interactions: Principles and Protocols, Third Edition., this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including .in vivo. and genome-wide interaction tech作者: Myocyte 時間: 2025-3-28 11:46
Emil Bergstr?m,Pontus W?rnest?led in the presence of the protein will feature blank regions (indicating protection) and/or enhanced cleavage sites (indicating increased availability). This technique can furthermore reveal if multiple sites for a DNA-binding protein are present on a same fragment, and allow the comparison of their respective affinities.作者: Stress 時間: 2025-3-28 15:43
Artificial Intelligence in Healthified factor or recombinant protein in vitro. As methylation protection is the in vitro equivalent of in vivo genomic footprinting, a direct comparison between in vivo and in vitro footprints can be made.作者: cleaver 時間: 2025-3-28 20:19
Bilash Dash,Tianhua Chen,Richard Hillring the footprinting reaction pose significant limitations. These limitations can be circumvented by combining the advantages of EMSA, with the subsequent exposure of the resolved DNA–protein complex(es) to the chemical nuclease 1,10-phenanthroline–copper ion (OP–Cu) while they are still embedded in the polyacrylamide matrix (.).作者: DIS 時間: 2025-3-29 01:48 作者: CRUC 時間: 2025-3-29 06:42 作者: Canopy 時間: 2025-3-29 07:14 作者: 小教堂 時間: 2025-3-29 14:07
Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting,and detected by their ability to bind radiolabeled DNA. The protein(s) interacting with the labeled DNA is visualized by autoradiography. This technique was used in our laboratory to visualize the metal regulatory consensus sequence-binding protein MTF-1 in L cell crude nuclear extracts.作者: 螢火蟲 時間: 2025-3-29 16:31 作者: transplantation 時間: 2025-3-29 23:03 作者: 圍巾 時間: 2025-3-30 00:02
Roberto Gatta,Stefania Orini,Mauro Vallatimplified by the polymerase chain reaction and is submitted to further rounds of selection. Each round of selection increases the proportion of sequences that are avidly bound by the protein of interest. The cloning and sequencing of these sequences finally completes their identification.作者: 飛來飛去真休 時間: 2025-3-30 06:02
Exonuclease III Footprinting on Immobilized DNA Templates,streptavidin-coated magnetic particles, information on the precise position of a DNA bound protein is available within a few hours. The position of the archaeal RNA polymerase at different stages of transcription in the . in vitro transcription system was analyzed by this method.作者: 安撫 時間: 2025-3-30 08:48
Uranyl Photofootprinting, acid complexes as well as potential (high affinity) cation (e.g., Mg.)-binding sites in folded nucleic acids. Finally, the cleavage modulation of duplex DNA reflects helix conformation in terms of minor groove width, due to preferential affinity/oxidation efficiency for such regions of the DNA helix.作者: cochlea 時間: 2025-3-30 12:39
Identification of Nucleic Acid High-Affinity Binding Sequences of Proteins by SELEX,mplified by the polymerase chain reaction and is submitted to further rounds of selection. Each round of selection increases the proportion of sequences that are avidly bound by the protein of interest. The cloning and sequencing of these sequences finally completes their identification.作者: 沉積物 時間: 2025-3-30 20:01 作者: Spirometry 時間: 2025-3-30 22:22 作者: placebo-effect 時間: 2025-3-31 03:01 作者: 變白 時間: 2025-3-31 05:53
The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Strne. The combination of both reagents gives excellent coverage of all sequence regions of DNA. Because reaction requires unstacking, the two reagents detect both melted regions and regions that are unstacked because of other distortions such as bending. Permanganate has the additional advantage that it can be utilized in living cells.作者: observatory 時間: 2025-3-31 12:14 作者: ANTI 時間: 2025-3-31 15:59 作者: 無聊的人 時間: 2025-3-31 21:23
Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates Within Model Chromatin Complexes,作者: Breach 時間: 2025-3-31 21:45
Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto South作者: 支架 時間: 2025-4-1 02:26 作者: coalition 時間: 2025-4-1 06:56 作者: 節(jié)約 時間: 2025-4-1 11:24
Filter-Binding Assays,The concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.作者: 相信 時間: 2025-4-1 16:13
Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions,henotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be作者: Relinquish 時間: 2025-4-1 22:19 作者: originality 時間: 2025-4-2 00:01
Samuel Romine,Joshua Jensen,Robert BallThe concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.作者: 債務 時間: 2025-4-2 06:03
R. Shoureshi,M. Wheeler,L. Brackneyhenotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be作者: creditor 時間: 2025-4-2 07:34
Book 2009Latest editionry protocols, and notes on troubleshooting and avoiding known pitfalls....Comprehensive and authoritative, .DNA-Protein Interactions: Principles and Protocols, Third Edition. serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research..
