標(biāo)題: Titlebook: DNA and RNA Profiling in Human Blood; Methods and Protocol Peter Bugert Book 2009 Humana Press 2009 Blood cell antigens.Disease markers.Gen [打印本頁] 作者: 戲弄 時(shí)間: 2025-3-21 18:08
書目名稱DNA and RNA Profiling in Human Blood影響因子(影響力)
書目名稱DNA and RNA Profiling in Human Blood影響因子(影響力)學(xué)科排名
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書目名稱DNA and RNA Profiling in Human Blood網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱DNA and RNA Profiling in Human Blood被引頻次
書目名稱DNA and RNA Profiling in Human Blood被引頻次學(xué)科排名
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書目名稱DNA and RNA Profiling in Human Blood讀者反饋
書目名稱DNA and RNA Profiling in Human Blood讀者反饋學(xué)科排名
作者: Junction 時(shí)間: 2025-3-21 23:35
Single Base Extension in Multiplex Blood Group Genotypingrnative strategy to phenotyping blood is to assay genomic DNA for the associated single nucleotide polymorphisms (SNPs). A multiplex PCR coupled with a single base oligonucleotide extension assay using genomic DNA can identify SNPs related to D, C/c, E/e, S/s, K/k, Kp., Fy., Fy0 (–33 promoter silenc作者: BRACE 時(shí)間: 2025-3-22 00:51 作者: 行乞 時(shí)間: 2025-3-22 04:34 作者: Vaginismus 時(shí)間: 2025-3-22 09:19 作者: 故意釣到白楊 時(shí)間: 2025-3-22 13:41
Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevances, cancer, or infections and genetically determined susceptibility to danger signals may influence the development of inflammatory diseases. Members of the ‘toll-like receptor’ (TLR) family are pivotal molecules in the activation of the innate immune system and specifically recognize structurally co作者: 故意釣到白楊 時(shí)間: 2025-3-22 20:38 作者: 類型 時(shí)間: 2025-3-22 22:57
High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Appriation involves differences in single DNA nucleotides, and is thus termed single nucleotide polymorphism (SNP). The need for improvement in throughput and reliability of traditional techniques makes it necessary to develop new technologies. Thus the past few years have witnessed an extraordinary su作者: 閹割 時(shí)間: 2025-3-23 03:30
Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeadsn genetic variation and disease. Discoveries made by such whole-genome association studies often spur further interest in surveying more focused subsets of SNPs for validation or research purposes. Here we describe a new SNP genotyping platform that is flexible in assay content and multiplexing (up 作者: 娘娘腔 時(shí)間: 2025-3-23 07:24 作者: 符合你規(guī)定 時(shí)間: 2025-3-23 11:28 作者: 楓樹 時(shí)間: 2025-3-23 16:21
RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysisemic diseases as well as the development of biomarkers for drug development. Since most cellular components of peripheral blood are specialized to quickly respond to exogenous stimuli, sample procurement approaches are required that reduce the overall impact of ex vivo changes in gene expression due作者: Cholecystokinin 時(shí)間: 2025-3-23 21:52 作者: Neutropenia 時(shí)間: 2025-3-24 02:05 作者: 蛤肉 時(shí)間: 2025-3-24 03:12
Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGnscripts. Taken together, these mRNAs constitute a platelet transcriptome. Platelets have a unique and reproducible transcript profile, which includes ～1,600–3,000 individual transcripts. In this chapter, we will focus on platelet purification and on transcript profiling using an Affymetrix microarr作者: 2否定 時(shí)間: 2025-3-24 06:51
Artificial Intelligence in Design ’00 group antigens. To efficiently respond to the numerous demands made for hemolytic disease of the newborn cases and polytransfused patients, we designed an inexpensive colorimetric high-throughput method to genotype several blood group antigens rapidly. Three simple steps are required to perform thi作者: 名詞 時(shí)間: 2025-3-24 12:42 作者: Arresting 時(shí)間: 2025-3-24 14:49
Lorenzo Mandow,José Luis Pérez De La Cruzor example, recently polytransfused patients, patients with positive direct human antiglobulin tests, and hemolytic disease of the newborn. The traditional polymerase chain reaction techniques are slow and sometimes difficult to carry out and interpret. Thus there is a need for the development and v作者: mercenary 時(shí)間: 2025-3-24 21:40
https://doi.org/10.1007/978-94-017-0795-4gy to type 6 common alleles (A1, A2, B, 01, 01?V, and 02) of the ABO blood group system, the high specificity and sensitivity make it suitable also in forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutatio作者: animated 時(shí)間: 2025-3-25 01:35
Artificial Intelligence in Design ’02method may, however, be adapted for the simultaneous analysis of up to 100 markers (50 biallelic SNPs) in a single reaction. In the method described, the targets of interest are amplified by single-tube multiplex PCR using six primer sets followed by single-tube multiplex allele-specific primer exte作者: Crepitus 時(shí)間: 2025-3-25 04:27 作者: 對(duì)待 時(shí)間: 2025-3-25 08:27
Representational Flexibility for Designarge number of allelic variants of these loci ensuring the high frequency of heterozygous genotypes observed in human populations. Molecular techniques, including sequencing, are capable of precisely defining . alleles. Sequencing by synthesis methodology employed by pyrosequencing represents a comp作者: Nebulous 時(shí)間: 2025-3-25 15:25 作者: Invertebrate 時(shí)間: 2025-3-25 18:20
Artificial Intelligence in Design ’92n genetic variation and disease. Discoveries made by such whole-genome association studies often spur further interest in surveying more focused subsets of SNPs for validation or research purposes. Here we describe a new SNP genotyping platform that is flexible in assay content and multiplexing (up 作者: ARCH 時(shí)間: 2025-3-25 20:55
Domain-Independent Design Systemtal D type in women with anti-D makes management of the pregnancy much easier and avoids unnecessary procedures in those women with a D-negative fetus. Fetal D typing can be performed by detection of an . gene in cell-free DNA in the plasma of D-negative pregnant women. The technology involves real-作者: analogous 時(shí)間: 2025-3-26 03:36 作者: 送秋波 時(shí)間: 2025-3-26 08:15 作者: 胡言亂語 時(shí)間: 2025-3-26 12:17
B. Dave,G. Schmitt,B. Faltings,I. Smithation. It is particularly suited for clinical samples yielding limited amounts of RNA. Unlike closed systems like microarray-based platforms, DD-PCR can be used to detect expression changes in known and novel transcripts, alternate splice products and to identify non-human transcripts. This chapter 作者: 殘廢的火焰 時(shí)間: 2025-3-26 15:04
A. Giretti,L. Spalazzi,M. Lemmafect, can identify differences in the abundance of specific transcripts that vary in a population-dependent manner. There are a variety of methods for identifying differentially expressed genes, including microarray, SAGE, qRT-PCR, and DDGE. This protocol describes a potentially less sensitive yet r作者: nascent 時(shí)間: 2025-3-26 19:31 作者: 挖掘 時(shí)間: 2025-3-26 23:26
Peter BugertDetails numerous techniques to perform blood group genotyping, covering prenatal blood grouping from maternal plasma to multiplex HLA genotyping.Presents not only established but also cutting-edge dev作者: 暴行 時(shí)間: 2025-3-27 01:27
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260219.jpg作者: 敲詐 時(shí)間: 2025-3-27 08:21
https://doi.org/10.1007/978-1-59745-553-4Blood cell antigens; Disease markers; Genotyping; High-throughput multiplex approaches; Megakaryocytes; P作者: Regurgitation 時(shí)間: 2025-3-27 11:26
DNA and RNA Profiling in Human Blood978-1-59745-553-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FIN 時(shí)間: 2025-3-27 16:21
Lorenzo Mandow,José Luis Pérez De La CruzMost human platelet alloantigen (HPA) systems comprise biallelic single nucleotide polymorphisms in genes encoding major membrane glycoproteins. Genotyping for these systems is required in the investigation of patients with suspected HPA antibodies and for the provision of compatible blood products from HPA-typed donor panel populations.作者: 無聊的人 時(shí)間: 2025-3-27 20:29
Real-Time PCR Assays for High-Throughput Human Platelet Antigen TypingMost human platelet alloantigen (HPA) systems comprise biallelic single nucleotide polymorphisms in genes encoding major membrane glycoproteins. Genotyping for these systems is required in the investigation of patients with suspected HPA antibodies and for the provision of compatible blood products from HPA-typed donor panel populations.作者: Accrue 時(shí)間: 2025-3-27 23:14
Artificial Intelligence in Design ’02 the Luminex xMAP?ledR system. The 12 universal tag sequences used in the assay derive from a set of 100 universal tags which have been designed to be isothermal and have been empirically validated to show that mismatch hybridization events are minimal. The method is suitable for cost-effective high-throughput clinical genotyping applications.作者: STERN 時(shí)間: 2025-3-28 03:23 作者: 煩憂 時(shí)間: 2025-3-28 09:44
Artificial Intelligence in Design ’92digitally inscribed VeraCode microbeads to allow streamlined workflow, rapid detection, unparalleled data reproducibility and consistency. Thus, it is a highly valuable tool for biomarker research and validation, pharmaceutical development, as well as the development of molecular diagnostic tests.作者: 使糾纏 時(shí)間: 2025-3-28 14:11
Domain-Independent Design Systemin multiplex with the . exon 5 test provides an internal control for the presence of fetal DNA when the fetus is male. Fetal D typing has become the standard of care in England in pregnant women with a significant level of anti-D.作者: 雜役 時(shí)間: 2025-3-28 15:02 作者: absorbed 時(shí)間: 2025-3-28 21:52 作者: 濕潤 時(shí)間: 2025-3-29 02:45
Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance functionally characterized and in part linked to multiple diseases. Here we report several protocols for Pyrosequencing?ledR approaches to genotype functionally relevant SNPs in TLR-genes and further molecules of the innate immune system.作者: Asymptomatic 時(shí)間: 2025-3-29 04:39 作者: 賠償 時(shí)間: 2025-3-29 08:55
The Use of Maternal Plasma for Prenatal RhD Blood Group Genotypingin multiplex with the . exon 5 test provides an internal control for the presence of fetal DNA when the fetus is male. Fetal D typing has become the standard of care in England in pregnant women with a significant level of anti-D.作者: 易發(fā)怒 時(shí)間: 2025-3-29 13:21 作者: fastness 時(shí)間: 2025-3-29 15:45
Multiplex ABO Genotyping by Minisequencing forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutations are readily investigated by the addition of new extension primers in the minisequencing multiplex reaction.作者: 裂口 時(shí)間: 2025-3-29 22:23 作者: 過份艷麗 時(shí)間: 2025-3-30 02:29 作者: CAND 時(shí)間: 2025-3-30 07:11 作者: 使人入神 時(shí)間: 2025-3-30 12:10
Book 2009Profiling in Human Blood: Methods and Protocols., leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens thr作者: Tdd526 時(shí)間: 2025-3-30 16:21
Real-Time PCR Assays for High-Throughput Blood Group Genotypingalidation of rapid and effective molecular methods. The genetic basis of the main alleles of the most important blood groups are known, but the frequencies vary in the different populations, thus for the genetic techniques to be efficient it is important to evaluate them, in order to adapt the molecular approaches.作者: legitimate 時(shí)間: 2025-3-30 17:50 作者: 防水 時(shí)間: 2025-3-30 22:24
Limited Computation, Unlimited Designse blood is provided for potential transfusion recipients. The potential to dramatically lower the incidence of alloimmunization and to avoid serious hemolytic complications of transfusions can be realized with the implementation of this technology.作者: FECK 時(shí)間: 2025-3-31 04:03 作者: 貪婪性 時(shí)間: 2025-3-31 06:08
Representational Flexibility for Designenerated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. In particular, we set up a universal array approach in combination with a PCR-LDR (polymerase chain reaction-ligation detection reaction) strategy for allele identification in the HLA gene.作者: Extricate 時(shí)間: 2025-3-31 09:16
A. Giretti,L. Spalazzi,M. Lemma is designed to isolate platelets from 20?ml of peripheral blood. This chapter provides detailed protocols for microarray and SAGE transcript profiling. We also discuss peculiarities of platelet purification, RNA isolation, and transcript profiling.作者: configuration 時(shí)間: 2025-3-31 13:33
PCR–ELISA for High-Throughput Blood Group Genotypingevant minor blood group antigens were adapted to this method and are described in this work: Rh (D, C, c, E, e), Kell (K, k), Duffy (Fy., Fy.), and Kidd (Jk., Jk.). Other blood group antigens could be easily tested this way as long as their molecular basis is well established.作者: bonnet 時(shí)間: 2025-3-31 18:47
Single Base Extension in Multiplex Blood Group Genotypingse blood is provided for potential transfusion recipients. The potential to dramatically lower the incidence of alloimmunization and to avoid serious hemolytic complications of transfusions can be realized with the implementation of this technology.作者: 圖畫文字 時(shí)間: 2025-4-1 01:33
Multiplex ,-Typing by Pyrosequencing proper matching of organ donor to recipient, the monitoring of . associated genetic risk to autoimmune diseases, population genetic studies, as well as evaluation of the genetics of human host–human pathogen interaction.作者: 雇傭兵 時(shí)間: 2025-4-1 05:27
High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Appenerated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. In particular, we set up a universal array approach in combination with a PCR-LDR (polymerase chain reaction-ligation detection reaction) strategy for allele identification in the HLA gene.作者: 創(chuàng)造性 時(shí)間: 2025-4-1 09:23
