標(biāo)題: Titlebook: DNA and RNA Isolation Techniques for Non-Experts; Akash Gautam Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁] 作者: 忠誠 時間: 2025-3-21 17:53
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書目名稱DNA and RNA Isolation Techniques for Non-Experts讀者反饋
書目名稱DNA and RNA Isolation Techniques for Non-Experts讀者反饋學(xué)科排名
作者: 橫截,橫斷 時間: 2025-3-21 21:19
2367-1114 r or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.978-3-030-94232-8978-3-030-94230-4Series ISSN 2367-1114 Series E-ISSN 2367-1122 作者: 衰老 時間: 2025-3-22 04:25
Applications: Physical Toy Examplesitated with ethanol. The concentration of PEG required for precipitation is inversely proportional to the size of the DNA fragments. This method can also be used to extract DNA from bacteria and fungi.作者: 隱士 時間: 2025-3-22 08:31 作者: deciduous 時間: 2025-3-22 12:05
https://doi.org/10.1007/978-981-16-0415-7ethod is better as compared to other RNA isolation protocols as the experimenter gets a higher yield of RNA. But this method is slightly time-consuming and uses many harmful chemicals compared to the column-based methods of RNA isolation.作者: 無意 時間: 2025-3-22 13:44 作者: 無意 時間: 2025-3-22 17:03 作者: 四溢 時間: 2025-3-23 00:45 作者: 痛打 時間: 2025-3-23 05:27
C. Edwin Singh,J. Amar Pratap Singh usage of easy and low-cost DNA extraction technologies in large-scale gene polymorphism investigations. This chapter aims to examine the most successful way of extracting DNA using the salt-out method. This method is easy, quick, safe, and inexpensive. It may be utilized in medical laboratories and research facilities.作者: 有幫助 時間: 2025-3-23 08:58
https://doi.org/10.1007/978-3-031-12419-8ling of precipitated DNA fibers on the glass rod or stirring rod under cold temperature conditions. Though the obtained DNA is free from proteins and other impurities, further processing of this DNA might be required for specific molecular biological applications.作者: Headstrong 時間: 2025-3-23 13:42 作者: Prosaic 時間: 2025-3-23 17:37 作者: Panacea 時間: 2025-3-23 19:54 作者: opalescence 時間: 2025-3-23 23:50
DNA Isolation by Chelex Methodegradation by chelating metal ions. This method is particularly beneficial for forensic applications, but it is not appropriate for large-scale DNA extraction. The resulting single-stranded DNA is less stable, therefore, not suitable for long-term storage and RFLP analysis.作者: 性學(xué)院 時間: 2025-3-24 05:30
DNA Isolation by Lysozyme and Proteinase Kis widely used in microbiology research, production of recombinant DNAs, biotechnology-based industries, and medical diagnosis where the presence of microbial DNA confirms the pathogenicity of a particular disease.作者: AER 時間: 2025-3-24 06:39
Isolation of DNA from Blood Samples by?Salting Method usage of easy and low-cost DNA extraction technologies in large-scale gene polymorphism investigations. This chapter aims to examine the most successful way of extracting DNA using the salt-out method. This method is easy, quick, safe, and inexpensive. It may be utilized in medical laboratories and research facilities.作者: Consequence 時間: 2025-3-24 11:32
DNA Extraction by Spooling Methodling of precipitated DNA fibers on the glass rod or stirring rod under cold temperature conditions. Though the obtained DNA is free from proteins and other impurities, further processing of this DNA might be required for specific molecular biological applications.作者: MORT 時間: 2025-3-24 17:39 作者: 希望 時間: 2025-3-24 20:40 作者: 教育學(xué) 時間: 2025-3-25 00:11
Philip Quinn,Wenxin Feng,Shumin Zhaitranded and depending upon the role it plays, it may be genetic or non-genetic. The non-genetic RNAs like mRNA, rRNA, tRNA, snRNA, and miRNA play various functional roles. Apart from being a storehouse of genetic information, nucleic acids also undergo mutations that lead to the expression of new traits and serve as the foundation for evolution.作者: 脫毛 時間: 2025-3-25 07:11
Xucong Zhang,Seonwook Park,Anna Maria Feit common steps and general biochemicals which are regularly used in the isolation steps. This chapter provides an overview of all those general steps along with the basic principles behind applying these steps during isolation protocols.作者: Sleep-Paralysis 時間: 2025-3-25 09:56 作者: 熱情贊揚 時間: 2025-3-25 14:00
Lecture Notes in Computer Scienceanimal tissues in resource-constrained environments. Therefore, this method is most suitable for the detection of contaminations in food. The major disadvantage of this approach is that it might result in highly diluted DNA, which requires subsequent concentration by using DNA-binding columns or silica-coated magnetic beads.作者: folliculitis 時間: 2025-3-25 19:19
https://doi.org/10.1007/978-3-031-27019-2ter removing out the aqueous (unprecipitated) sample. In spite of the abovementioned advantages, this technique might not be suitable for isolating the lower concentration of RNAs and different types of RNA other than mRNAs, as evident by the different contradictory studies.作者: exceptional 時間: 2025-3-25 22:30
Basic Idea About Nucleic Acids for Nonexpertstranded and depending upon the role it plays, it may be genetic or non-genetic. The non-genetic RNAs like mRNA, rRNA, tRNA, snRNA, and miRNA play various functional roles. Apart from being a storehouse of genetic information, nucleic acids also undergo mutations that lead to the expression of new traits and serve as the foundation for evolution.作者: 洞穴 時間: 2025-3-26 03:02
General Steps During Isolation of DNA and RNA common steps and general biochemicals which are regularly used in the isolation steps. This chapter provides an overview of all those general steps along with the basic principles behind applying these steps during isolation protocols.作者: 緯度 時間: 2025-3-26 04:31
Phenol-Chloroform DNA Isolation Methodnitial steps. Despite all these advantages, this method uses different dangerous organic solvents and hence should be carefully handled. Moreover, this method is laborious and time-consuming compared to the other methods available, for example, column-based isolation of DNA.作者: BROTH 時間: 2025-3-26 11:53
DNA Isolation by Hydrophilic Ionic Liquid Treatmentanimal tissues in resource-constrained environments. Therefore, this method is most suitable for the detection of contaminations in food. The major disadvantage of this approach is that it might result in highly diluted DNA, which requires subsequent concentration by using DNA-binding columns or silica-coated magnetic beads.作者: 鞠躬 時間: 2025-3-26 12:57
Lithium Chloride-Based Isolation of RNAter removing out the aqueous (unprecipitated) sample. In spite of the abovementioned advantages, this technique might not be suitable for isolating the lower concentration of RNAs and different types of RNA other than mRNAs, as evident by the different contradictory studies.作者: 他一致 時間: 2025-3-26 19:08
Book 2022 a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.作者: 陶醉 時間: 2025-3-26 22:01
Philip Quinn,Wenxin Feng,Shumin ZhaiNA is mainly involved in the processing (transcription) and manifestation (translation) of this genetic information. They are unbranched macromolecules made up of monomeric units called nucleotides which are linked together by phosphodiester bonds. Nucleotides consist of a pentose sugar, a nitrogeno作者: paltry 時間: 2025-3-27 02:09
Xucong Zhang,Seonwook Park,Anna Maria Feiterial is present either as DNA or RNA inside cells. The biological study of these DNA or RNA molecules for various purposes requires their isolation from their native location to the in vitro conditions. Over the years, scientists have developed different extraction techniques for DNA and RNA from a作者: 死亡 時間: 2025-3-27 08:43 作者: Harness 時間: 2025-3-27 10:30 作者: 完成才會征服 時間: 2025-3-27 17:29
Poulami Majumder,Partha Pratim Ray tissues, plant extracts, viruses, and bacteria. It is advantageous over other extraction techniques such as less time consumption, economical, energy-efficient, automated operation, prevention from cross-contamination, and high yield. It is based on the principle of binding nucleic acid to immobili作者: artless 時間: 2025-3-27 19:50 作者: 神化怪物 時間: 2025-3-27 23:19 作者: guardianship 時間: 2025-3-28 06:04 作者: 砍伐 時間: 2025-3-28 10:12 作者: 摘要記錄 時間: 2025-3-28 14:11
Applications: Physical Toy Examplesdoes not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH?10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to 作者: 阻礙 時間: 2025-3-28 17:38 作者: 外表讀作 時間: 2025-3-28 18:50 作者: BRACE 時間: 2025-3-29 00:52 作者: fulmination 時間: 2025-3-29 04:40
https://doi.org/10.1007/978-3-031-12419-8 of around 200?billion cells will be challenging and tedious. But the extraction of DNA by spooling method helps the experimenter isolate such a large quantity and long fibers of DNA conveniently. The nonexperts, in particular, can visualize the white translucent DNA fibers after spooling in this te作者: 怪物 時間: 2025-3-29 09:02
Artificial Intelligence for Societal Issues, hair or nails, soil, fungal growth, and viruses. It is advantageous over other extraction strategies such as cost-effective, rapid, eco-friendly, and high yield. This strategy is based upon the principle of paramagnetism or superparamagnetism. An external magnetic field is applied to a solution co作者: 英寸 時間: 2025-3-29 14:54
Intelligent Systems Reference Libraryon of the new organism and their categorization, diagnosing patients with genetic mutations, and also the expression levels of specific genes. Therefore, the scientific fraternity has devised new and innovative methods for the isolation of the said biomolecules. However, although a little old, the d作者: 離開 時間: 2025-3-29 19:02
Artificial Intelligence for Societal Issuesh which DNA isolation can be done. Some techniques claim to provide highly pure DNA but trade-off with the yield, and others high at the yield but compromise purity. The technique of DNA extraction from agarose gel through paper strips is advantageous over its counterparts in the low cost and less i作者: GAVEL 時間: 2025-3-29 19:52
Akash GautamAccessible guide for junior researchers and those in adjacent fields beginning interdisciplinary research.Presents basic concepts, step-by-step instructions, and abundant illustrations.Addresses the b作者: Initiative 時間: 2025-3-30 02:45 作者: 建筑師 時間: 2025-3-30 05:57 作者: liaison 時間: 2025-3-30 11:49 作者: 后來 時間: 2025-3-30 14:42
978-3-030-94232-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl作者: Merited 時間: 2025-3-30 19:11
Basic Idea About Nucleic Acids for NonexpertsNA is mainly involved in the processing (transcription) and manifestation (translation) of this genetic information. They are unbranched macromolecules made up of monomeric units called nucleotides which are linked together by phosphodiester bonds. Nucleotides consist of a pentose sugar, a nitrogeno作者: 裂口 時間: 2025-3-30 23:31
General Steps During Isolation of DNA and RNAerial is present either as DNA or RNA inside cells. The biological study of these DNA or RNA molecules for various purposes requires their isolation from their native location to the in vitro conditions. Over the years, scientists have developed different extraction techniques for DNA and RNA from a作者: AMOR 時間: 2025-3-31 04:33
Phenol-Chloroform DNA Isolation Methodmolecular weight DNA from any biological sample using a mixture of phenol-chloroform-isoamyl alcohol. The basic principle involves the separation of DNA, RNA, and proteins in a sample, based on the differential solubilities of these molecules in different immiscible liquids. The isolated DNA can be 作者: Humble 時間: 2025-3-31 06:58
RNA Isolation by the Guanidinium-Acid-Phenol Methodwn as the Trizol method of RNA isolation. During this method, the sample is treated with guanidinium isothiocyanate and acidic phenol, and chloroform. Here guanidinium isothiocyanate acts as a powerful protein denaturant and deactivates the RNases, whereas the acidic phenol-chloroform helps in the p作者: CHASM 時間: 2025-3-31 09:24
Spin Column-Based Isolation of Nucleic Acid tissues, plant extracts, viruses, and bacteria. It is advantageous over other extraction techniques such as less time consumption, economical, energy-efficient, automated operation, prevention from cross-contamination, and high yield. It is based on the principle of binding nucleic acid to immobili作者: UTTER 時間: 2025-3-31 15:47
Isolation of Plasmid DNA by Alkaline Lysisthe chromosomal DNA. They provide antibiotic resistance and hence better adaptation qualities to these microorganisms to survive in different environmental conditions. Molecular biologists harness these properties of plasmid DNA and use them for several applications such as sequencing, screening clo作者: 阻撓 時間: 2025-3-31 19:36
DNA Isolation by Hydrophilic Ionic Liquid Treatments only a few minutes to complete. Ionic liquids can dissolve biomass and release DNA molecules from tissues and cells. DNA released in the supernatant may be used as such for downstream applications. The extraction procedure includes mixing ionic liquids with starting sample at a high temperature (6作者: 怒目而視 時間: 2025-4-1 00:47 作者: 神圣不可 時間: 2025-4-1 05:52 作者: MOCK 時間: 2025-4-1 06:48
DNA Isolation by Chelex Methoddoes not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH?10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to 作者: Addictive 時間: 2025-4-1 12:48
