標題: Titlebook: DNA Viruses; Methods and Protocol Paul M. Lieberman Book 2005 Humana Press 2005 DNA.PCR.Termination.cell lines.electron microscopy.gene exp [打印本頁] 作者: bradycardia 時間: 2025-3-21 20:01
書目名稱DNA Viruses影響因子(影響力)
書目名稱DNA Viruses影響因子(影響力)學科排名
書目名稱DNA Viruses網絡公開度
書目名稱DNA Viruses網絡公開度學科排名
書目名稱DNA Viruses被引頻次
書目名稱DNA Viruses被引頻次學科排名
書目名稱DNA Viruses年度引用
書目名稱DNA Viruses年度引用學科排名
書目名稱DNA Viruses讀者反饋
書目名稱DNA Viruses讀者反饋學科排名
作者: Fabric 時間: 2025-3-21 22:10
AI U.S. Policies and Regulationstion. The reporter gene employed was green fluorescent protein (GFP) or secreted alkaline phosphatase (SEAP), whose assays offer real-time detection or quantification, respectively. This cell-based assay is simple, rapid, sensitive, specific, and quantitative and serves as a phenotypic method for determination of antiviral susceptibilities.作者: exceed 時間: 2025-3-22 03:41 作者: 令人苦惱 時間: 2025-3-22 04:46 作者: GET 時間: 2025-3-22 10:28 作者: 怪物 時間: 2025-3-22 16:28
https://doi.org/10.1007/978-3-031-29126-5e virions and are therefore valuable tools for the study of papillomavirus-cell interactions. The methods described can be adopted for other nonenveloped DNA viruses and may be useful for gene transfer.作者: 怪物 時間: 2025-3-22 18:11 作者: 激怒某人 時間: 2025-3-23 00:49 作者: INCUR 時間: 2025-3-23 02:17 作者: 生氣的邊緣 時間: 2025-3-23 06:23 作者: 環(huán)形 時間: 2025-3-23 12:06
Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cellse virions and are therefore valuable tools for the study of papillomavirus-cell interactions. The methods described can be adopted for other nonenveloped DNA viruses and may be useful for gene transfer.作者: graphy 時間: 2025-3-23 15:59 作者: 軟弱 時間: 2025-3-23 21:11
https://doi.org/10.1007/978-3-030-22971-9id. Difference maps calculated by subtracting the quasi-atomic model from the cryoelectron microscopy map reveal the molecular envelope of those capsid components whose atomic structure is unknown. A better understanding of the complex interactions involved in capsid assembly, stabilization, and disassembly is thus achieved.作者: inchoate 時間: 2025-3-24 00:49 作者: Crepitus 時間: 2025-3-24 04:06 作者: 未成熟 時間: 2025-3-24 07:51
1064-3745 ied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly作者: FLINT 時間: 2025-3-24 11:59 作者: 仇恨 時間: 2025-3-24 17:54 作者: FORGO 時間: 2025-3-24 19:45 作者: Ancestor 時間: 2025-3-25 01:59 作者: 鋸齒狀 時間: 2025-3-25 05:29
Book 2005omplex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.作者: Bucket 時間: 2025-3-25 10:11 作者: 不透明 時間: 2025-3-25 13:30 作者: Visual-Field 時間: 2025-3-25 19:07 作者: Synchronism 時間: 2025-3-25 20:09 作者: 不可接觸 時間: 2025-3-26 01:55
DNA Viruses978-1-59259-848-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 樣式 時間: 2025-3-26 07:33
Artificial Intelligence for Designing Gamesain reaction (RT-PCR), PCR, real-time RT-PCR, and real-time PCR methods. Amplification of the E6 and E7 oncoproteins of HPV16 is described in detail, and primers and probes that can be used to amplify these oncogenes are described. Techniques to quantify these oncogenes in infected human tissue spec作者: stratum-corneum 時間: 2025-3-26 10:45 作者: 擦試不掉 時間: 2025-3-26 13:24
Penousal Machado,Juan Romero,Gary Greenfieldrus. As a control for RNA integrity, the low-copy-number transcript derived from U1 A snRNP, a cellular housekeeping gene, is coamplified. Copy DNA (cDNA) for these nine targets is simultaneously synthesized in a gene-specific, multiprimed cDNA reaction, which strongly reduces the amount of required作者: 刻苦讀書 時間: 2025-3-26 20:20 作者: 手勢 時間: 2025-3-26 23:07
AI U.S. Policies and Regulationsm latency in a number of nonhuman species, including mice. This provides a unique opportunity to study the complex lytic-latent cycle of a human neurotropic virus in a mouse model. This chapter details basic methods for inducing and quantifying reactivation, with emphasis on the first strategy for d作者: grovel 時間: 2025-3-27 03:18 作者: fidelity 時間: 2025-3-27 07:29
Historical Sketch of Artificial Intelligence and function. AFM allows the direct visualization of viruses in a hydrated state and can probe surface topography in unrivaled detail. Moreover, AFM can be used to elucidate dynamic processes associated with the life cycle of viruses in vitro. It can readily produce high-resolution, nonaveraged, si作者: irritation 時間: 2025-3-27 12:24 作者: APRON 時間: 2025-3-27 14:15 作者: 琺瑯 時間: 2025-3-27 20:56
a SWIPT-Enabled Wireless Systeming primary monoclonal antibodies from the same species. The optimized three-layer indirect immunogold-labeled cryosection electron microscopy described is recommended for studies of virus-cell interactions, because: (.) it is a simple and reproducible method; (.) colloidal gold markers are electron作者: 耕種 時間: 2025-3-28 01:53 作者: cuticle 時間: 2025-3-28 02:44
Yuchen Li,Cong Zhou,Shuo Shi,Zhenyu Xu progressive multifocal leukoencephalopathy (PML). It has been reported that sialic acids play a pivotal role in hemagglutination of red blood cells and entry into host cells of JCV and that JCV can enter a wide variety of cell types and localize to the nuclei. The outer shell of the JCV virion comp作者: Pudendal-Nerve 時間: 2025-3-28 07:22
Jiahao Dai,Yuhui Wang,Huijie Zhu,Yongji Rens two distinct steps. The first is characterized by the binding of viral envelope glycoproteins to host cellular receptors. After binding, the viral membrane and the cellular membrane fuse. Without both binding and fusion, the virus is not able to enter the host target cell efficiently. Combined wit作者: 脖子 時間: 2025-3-28 11:33
https://doi.org/10.1007/978-3-031-29126-5of vaccinia viruses recombinant for the major and minor HPV capsid proteins, L1 and L2, respectively; (.) the transfection of Cos7 cells with a marker plasmid replicating to high copy numbers; (.) the expression of L1 and L2 using the vaccinia virus expression system; (.) the extraction, purificatio作者: arousal 時間: 2025-3-28 15:16 作者: 熒光 時間: 2025-3-28 22:35
Viral Detectionain reaction (RT-PCR), PCR, real-time RT-PCR, and real-time PCR methods. Amplification of the E6 and E7 oncoproteins of HPV16 is described in detail, and primers and probes that can be used to amplify these oncogenes are described. Techniques to quantify these oncogenes in infected human tissue spec作者: 聲音刺耳 時間: 2025-3-29 01:44
Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Target, or plasma. This assay is based on amplification of a highly conserved 213-bp region of the . gene, a single-copy gene of EBV required for maintenance of the EBV genome within the infected host cell. For real-time detection of amplicons, two internal hybridization probes are added, labeled with the作者: 運動吧 時間: 2025-3-29 07:02
Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprrus. As a control for RNA integrity, the low-copy-number transcript derived from U1 A snRNP, a cellular housekeeping gene, is coamplified. Copy DNA (cDNA) for these nine targets is simultaneously synthesized in a gene-specific, multiprimed cDNA reaction, which strongly reduces the amount of required作者: miniature 時間: 2025-3-29 08:54
Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells ected cells within a given population are expressing the viral genes . Q-K, ., ., ., ., and the .. Because this technique involves limiting dilution analysis, it is possible to define which viral transcription programs are being used at the single-cell level. This assay takes 3-4 d to complete and i作者: COWER 時間: 2025-3-29 12:00
Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Tranm latency in a number of nonhuman species, including mice. This provides a unique opportunity to study the complex lytic-latent cycle of a human neurotropic virus in a mouse model. This chapter details basic methods for inducing and quantifying reactivation, with emphasis on the first strategy for d作者: 泥沼 時間: 2025-3-29 15:51
Reporter Cell Lines for the Detection of Herpes Simplex Virusesechniques. However, virus culture is generally a slower process, as it inevitably takes the period of a full replication cycle of a given virus. A genetically modified cell culture with a virus-inducible marker is described here, using a frequently isolated DNA virus (herpes simplex virus) as a mode作者: 煤渣 時間: 2025-3-29 23:26 作者: 暴發(fā)戶 時間: 2025-3-30 00:06
Studying the Structure of Large Viruses With Multiresolution Imagingirements of protein crystallography. The methodology consists in fitting the atomic structures of capsid components, independently solved, to medium-resolution, 3D maps of the complete virion, obtained by cryoelectron microscopy and image processing. On combining the two kinds of imaging data, one m作者: 健談的人 時間: 2025-3-30 08:05
Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence other cell biology studies because: (.) it is a simple, rapid, sensitive, and reproducible technique; (.) phase-contrast microscopy is unnecessary; (.) contrast is optimal without blurring the fluorescent labeling; (.) autofluorescence is minimal, even in fixed cells; (.) background staining is min作者: 著名 時間: 2025-3-30 11:03 作者: Physiatrist 時間: 2025-3-30 12:56 作者: 胰臟 時間: 2025-3-30 19:59
The JC Virus-Like Particle Overlay Assay progressive multifocal leukoencephalopathy (PML). It has been reported that sialic acids play a pivotal role in hemagglutination of red blood cells and entry into host cells of JCV and that JCV can enter a wide variety of cell types and localize to the nuclei. The outer shell of the JCV virion comp作者: 改革運動 時間: 2025-3-30 21:08 作者: Obvious 時間: 2025-3-31 01:57 作者: 要求比…更好 時間: 2025-3-31 06:26 作者: Coma704 時間: 2025-3-31 10:36 作者: Allergic 時間: 2025-3-31 14:10
