標題: Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media [打印本頁] 作者: Nutraceutical 時間: 2025-3-21 20:08
書目名稱DNA Vaccines影響因子(影響力)
書目名稱DNA Vaccines影響因子(影響力)學科排名
書目名稱DNA Vaccines網(wǎng)絡公開度
書目名稱DNA Vaccines網(wǎng)絡公開度學科排名
書目名稱DNA Vaccines被引頻次
書目名稱DNA Vaccines被引頻次學科排名
書目名稱DNA Vaccines年度引用
書目名稱DNA Vaccines年度引用學科排名
書目名稱DNA Vaccines讀者反饋
書目名稱DNA Vaccines讀者反饋學科排名
作者: Countermand 時間: 2025-3-21 23:12
Strategies for Improving DNA Vaccine Performancestructure and easy manipulation, a wide range of strategies can be applied to improve DNA vaccine performance. This chapter discusses this topic in detail taking into account antigen/epitope selection and optimization, inclusion of intracellular targeting sequences and genetic adjuvants, and provision of T cell help.作者: gene-therapy 時間: 2025-3-22 01:11
Ali Movaghar,Mansour Jamzad,Hossein Asadiep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.作者: syncope 時間: 2025-3-22 04:55
Rafa? Grycuk,Kelton Costa,Rafa? Scherer we describe fabrication methods of microneedles and DNA vaccine loading methods on the microneedle surface. Furthermore, in the latter part of this chapter, in vivo test protocols for assessing the efficacy of gene delivery using microneedles are described.作者: addition 時間: 2025-3-22 11:05
Enhancement of Plasmid-Mediated Transgene Expressionep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.作者: 殺人 時間: 2025-3-22 16:22 作者: 殺人 時間: 2025-3-22 20:00
1064-3745 ts.Contains key notes and implementation advice from the exp.DNA Vaccines: Methods and Protocols, Third Edition .explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.? Featuring applications which should be of great value in moving vaccine作者: 弄污 時間: 2025-3-23 00:03 作者: 機構(gòu) 時間: 2025-3-23 02:08
Mostafa Borhani,Hassan Ghassemianaccines was tested firstly in vitro, in Chinese hamster ovary cells, through flow cytometry and real-time polymerase chain reaction analysis. Then, an essential in vivo study was performed in chickens, in order to evaluate the efficacy of DNA prototype vaccines, by measuring the antibody production by enzyme-linked immunosorbent assay.作者: 懲罰 時間: 2025-3-23 06:27
https://doi.org/10.1007/978-981-97-0341-8how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.作者: Heart-Attack 時間: 2025-3-23 09:51
https://doi.org/10.1007/978-3-030-61534-5 protocol that utilizes a simple tattooing device for the dermal delivery of plasmid DNA. This technique, called DNA tattooing, is capable of generating high frequencies of antigen-reactive T cells in mice and macaques.作者: 豪華 時間: 2025-3-23 13:56
Artificial Intelligence and Soft Computingstruction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.作者: milligram 時間: 2025-3-23 18:58
Piotr P?oński,Krzysztof Zaremba as a vaccine in an asthma mouse model. Attaching signal sequences has proven to be a successful way to manipulate and boost the immune responses following DNA immunization and also creating hypoallergenic DNA vaccines.作者: arousal 時間: 2025-3-23 22:30 作者: 解凍 時間: 2025-3-24 03:20 作者: NEXUS 時間: 2025-3-24 10:21 作者: 無情 時間: 2025-3-24 13:40 作者: airborne 時間: 2025-3-24 17:27
Protective and Therapeutic DNA Vaccination Against Allergic Diseases as a vaccine in an asthma mouse model. Attaching signal sequences has proven to be a successful way to manipulate and boost the immune responses following DNA immunization and also creating hypoallergenic DNA vaccines.作者: pacific 時間: 2025-3-24 22:53
Book 2014Latest edition of DNA vaccines.? Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.作者: Assemble 時間: 2025-3-25 02:11
Pharmaceutical Grade Large-Scale Plasmid DNA Manufacturing Process necessarily be produced under GMP..Besides such regulatory aspects up-scaling of the plasmid DNA production process from research laboratory scale (up to a few milligrams) to industrial scales (milligram to gram scales) is an issue that is addressed here.作者: Affection 時間: 2025-3-25 06:51 作者: 滑稽 時間: 2025-3-25 07:57
https://doi.org/10.1007/978-3-319-10849-0structure and easy manipulation, a wide range of strategies can be applied to improve DNA vaccine performance. This chapter discusses this topic in detail taking into account antigen/epitope selection and optimization, inclusion of intracellular targeting sequences and genetic adjuvants, and provision of T cell help.作者: DEFER 時間: 2025-3-25 14:20
DNA Vaccines978-1-4939-0410-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: albuminuria 時間: 2025-3-25 16:41
Reza Hallaj,Reza Mohseni,Kamran Kazemio make up for this limitation by altering plasmid construction through vector design innovations intended for enhancement of transgene expression and immunogenicity. The next-generation vectors also address safety issues such as selection markers. This chapter discusses (a) plasmid backbone design, 作者: 變色龍 時間: 2025-3-25 21:47 作者: 高度贊揚 時間: 2025-3-26 03:40 作者: synovial-joint 時間: 2025-3-26 04:31 作者: NIB 時間: 2025-3-26 11:28 作者: 舊式步槍 時間: 2025-3-26 12:49
Kusum Pandey,N. L. Kushwaha,K. G. Singhr presents methodologies for the design and cloning of synthetic antigen genes into RNA-OUT encoding antibiotic-free DNA vaccine vectors that are additionally optimized to improve protein expression, and immunogenicity, compared to alternative kanamycin-resistant vectors..First, antigen targeting co作者: 騎師 時間: 2025-3-26 18:14
Marek Palkowski,Wlodzimierz Bieleckige mammals and humans, an enhancing delivery technology is required. Electroporation is a physical technique which results in improved delivery of large molecules through the cell membrane. In the case of plasmid DNA, electroporation enhances both the uptake and expression of the delivered DNA. The 作者: CAMEO 時間: 2025-3-26 21:07 作者: 高度贊揚 時間: 2025-3-27 02:25 作者: GLUT 時間: 2025-3-27 08:58 作者: MIRTH 時間: 2025-3-27 11:20 作者: elucidate 時間: 2025-3-27 17:32 作者: anagen 時間: 2025-3-27 18:09
https://doi.org/10.1007/978-3-319-19369-4to human “Good Manufacturing Practice” (GMP) grade is mandatory, for GMP production of, e.g., viral vectors (AAV, etc.) the plasmid DNA used needs not necessarily be produced under GMP..Besides such regulatory aspects up-scaling of the plasmid DNA production process from research laboratory scale (u作者: Crayon 時間: 2025-3-28 00:49
Piotr P?oński,Krzysztof Zarembamunotherapy, which relies on the injection of alum-adsorbed protein extracts, DNA vaccines do not suffer from side effects such as anaphylaxis or therapy-induced IgE antibodies. In animal models, DNA vaccines have been demonstrated to prevent TH2 sensitization or balance an existing TH2-mediated all作者: Genome 時間: 2025-3-28 04:12
https://doi.org/10.1007/978-3-319-07173-2DNA delivery to for example skin and muscle can easily be performed with electroporation. The method is efficient, feasible, and inexpensive and the future possibilities are numerous. Here we present our protocol for gene transfection to mouse skin using naked plasmid DNA and electric pulses.作者: congenial 時間: 2025-3-28 08:49 作者: wangle 時間: 2025-3-28 13:23
Monica Rinaldi,Daniela Fioretti,Sandra IuresciaIncludes cutting-edge methods and protocols involving DNA vaccine applications.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exp作者: GAVEL 時間: 2025-3-28 17:37
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260210.jpg作者: 朦朧 時間: 2025-3-28 22:23 作者: 建筑師 時間: 2025-3-29 02:50 作者: debris 時間: 2025-3-29 04:43
A Blueprint for DNA Vaccine Designo make up for this limitation by altering plasmid construction through vector design innovations intended for enhancement of transgene expression and immunogenicity. The next-generation vectors also address safety issues such as selection markers. This chapter discusses (a) plasmid backbone design, 作者: Immunotherapy 時間: 2025-3-29 07:43
Enhancement of Plasmid-Mediated Transgene Expression as muscle disorders strengthen the fact that gene therapy could represent an alternative method to treat human diseases where conventional approaches are less effective..To improve transgene expression from plasmid vectors, DNA nuclear targeting sequences (DTSs) can be introduced in a vector backbo作者: Multiple 時間: 2025-3-29 15:22 作者: 違法事實 時間: 2025-3-29 19:24 作者: Sedative 時間: 2025-3-29 22:05
Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategiest been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is neede作者: COKE 時間: 2025-3-30 01:50
Development of Antibiotic-Free Selection System for Safer DNA Vaccinationr presents methodologies for the design and cloning of synthetic antigen genes into RNA-OUT encoding antibiotic-free DNA vaccine vectors that are additionally optimized to improve protein expression, and immunogenicity, compared to alternative kanamycin-resistant vectors..First, antigen targeting co作者: 羽飾 時間: 2025-3-30 04:25
DNA Vaccination in Skin Enhanced by Electroporationge mammals and humans, an enhancing delivery technology is required. Electroporation is a physical technique which results in improved delivery of large molecules through the cell membrane. In the case of plasmid DNA, electroporation enhances both the uptake and expression of the delivered DNA. The 作者: MOTTO 時間: 2025-3-30 10:08 作者: CRACK 時間: 2025-3-30 15:57 作者: convert 時間: 2025-3-30 19:22 作者: 不近人情 時間: 2025-3-30 20:53 作者: 無情 時間: 2025-3-31 02:02 作者: excrete 時間: 2025-3-31 05:42 作者: 統(tǒng)治人類 時間: 2025-3-31 12:20
Protective and Therapeutic DNA Vaccination Against Allergic Diseasesmunotherapy, which relies on the injection of alum-adsorbed protein extracts, DNA vaccines do not suffer from side effects such as anaphylaxis or therapy-induced IgE antibodies. In animal models, DNA vaccines have been demonstrated to prevent TH2 sensitization or balance an existing TH2-mediated all作者: 下垂 時間: 2025-3-31 14:00
Development of Antibiotic-Free Selection System for Safer DNA Vaccinationes into the NTC868 series RNA-OUT vector system are presented. The resultant antibiotic-free DNA vaccine vectors are a more potent, safer alternative to existing kanamycin resistance marker encoding vectors.作者: 集合 時間: 2025-3-31 20:34
