標題: Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA [打印本頁] 作者: 譴責 時間: 2025-3-21 16:38
書目名稱DNA Topoisomerases影響因子(影響力)
書目名稱DNA Topoisomerases影響因子(影響力)學科排名
書目名稱DNA Topoisomerases網(wǎng)絡公開度
書目名稱DNA Topoisomerases網(wǎng)絡公開度學科排名
書目名稱DNA Topoisomerases被引頻次
書目名稱DNA Topoisomerases被引頻次學科排名
書目名稱DNA Topoisomerases年度引用
書目名稱DNA Topoisomerases年度引用學科排名
書目名稱DNA Topoisomerases讀者反饋
書目名稱DNA Topoisomerases讀者反饋學科排名
作者: 字的誤用 時間: 2025-3-21 21:28
Artificial Intelligence XXXVIIIthod for creating the DNA substrate and using it as a tool to monitor the conformational changes at the topo II DNA gate. We detail how to collect and process fluorescence spectra to determine the FRET efficiency of the DNA substrate.作者: callous 時間: 2025-3-22 02:56
Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II,tranded DNA cleavage by monitoring the conversion of covalently closed supercoiled DNA to linear molecules. The assay is simple, straightforward, and does not require the use of radiolabeled substrates.作者: 殘暴 時間: 2025-3-22 05:43 作者: 外貌 時間: 2025-3-22 12:18
Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Hum作者: nutrition 時間: 2025-3-22 15:37 作者: nutrition 時間: 2025-3-22 20:18 作者: 休息 時間: 2025-3-22 23:02 作者: idiopathic 時間: 2025-3-23 04:00 作者: Habituate 時間: 2025-3-23 06:39
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260202.jpg作者: Allowance 時間: 2025-3-23 13:00 作者: Sleep-Paralysis 時間: 2025-3-23 17:00 作者: 確定方向 時間: 2025-3-23 21:27
Artificial Intelligence XXXVIIIrticularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.作者: epicardium 時間: 2025-3-24 00:27 作者: 五行打油詩 時間: 2025-3-24 06:13
Yu An,Qing Ye,Jiulin Guo,Ruihai Dongolution, chromosome condensation, and sister chromatid separation. Deficiencies in these enzymes are associated with diseases that result from genome instability. The last 10–15 years has seen a great deal of exciting research in the field of topoisomerase. Here we discuss a selection of the new the作者: Fibrillation 時間: 2025-3-24 06:40
Lecture Notes in Computer Scienceas been used for more than 30 years, the physical mechanism underlying the resolution of topological states remains poorly understood. However, electrophoretic methods remain the most robust and precise techniques for determining the local unwinding of DNA induced by the binding of proteins and smal作者: Flawless 時間: 2025-3-24 13:10 作者: mosque 時間: 2025-3-24 17:15 作者: 小丑 時間: 2025-3-24 20:23
Lecture Notes in Computer Scienceinactive mutant form of the enzyme. In the gel shift assay, the preference for binding to supercoiled plasmid DNA is detected in the presence of linear and nicked forms of the same DNA by a reduction in the mobility of the supercoiled plasmid during electrophoresis in agarose. The more quantitative 作者: irreparable 時間: 2025-3-25 00:36 作者: 國家明智 時間: 2025-3-25 05:18
Artificial Intelligence XXXVIIIrticularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.作者: 征服 時間: 2025-3-25 10:39 作者: 外觀 時間: 2025-3-25 15:43
Artificial Intelligence XXXVIIIbased on the immunoprecipitation of crosslinked chromatin (ChIP, chromatin immunoprecipitation), followed by DNA amplification and hybridization to high-density oligonucleotide arrays (Chip). Comparison of the signal intensities of immunoprecipitated and control fractions provides a measurement of t作者: BRAVE 時間: 2025-3-25 19:15
Ada Maria Barone,Emanuela Stagnotromeric chromatin during metaphase. The factors controlling these changes in enzyme distribution are largely unknown. Insight into Topo II dynamics could potentially be derived through genetic approaches in yeast. In practice, however, the small size and limited compaction of yeast chromosomes has 作者: 裝入膠囊 時間: 2025-3-25 21:21
Studies in Computational Intelligenceion of enzyme poisons specific for type I or type II DNA topoisomerases. Using the topoisomerase I poison camptothecin and the topoisomerase II poison VP16, the precise sites of interaction of these enzymes around the lamin B2 origin have been identified at different points in the cell cycle. The pr作者: 禮節(jié) 時間: 2025-3-26 04:04
Studies in Computational Intelligence double-strand breaks (DSBs). DNA damage, particularly induction of DSBs, manifests by phosphorylation of histone H2AX on .139 which is mediated by one of the protein kinases of the phosphoinositide kinase family, namely ATM, ATR, and/or DNA-PK. The presence of .-139 phosphorylated H2AX (γH2AX) is t作者: Excitotoxin 時間: 2025-3-26 05:32
Studies in Computational Intelligencelding the mitotic chromosome. Perturbed Topo II activity causes defects in chromosome segregation due to persistent catenations and aberrant DNA condensation during mitosis. Recently, novel . alleles in the yeast . revealed a checkpoint control that responds to perturbed Topo II activity. Described 作者: deadlock 時間: 2025-3-26 12:07 作者: 泥瓦匠 時間: 2025-3-26 16:18 作者: farewell 時間: 2025-3-26 18:41
Bahaaeddin Alareeni,Allam Hamdan) for use in studying the dynamics of Topo IIα in living cells. In previous studies, this plasmid was transfected into LLC-Pk cells, a porcine epithelial cell line that remains relatively flat during mitosis. After selection for stable integration, cells were cloned by serial dilution in microwells 作者: Intractable 時間: 2025-3-26 22:02 作者: Arboreal 時間: 2025-3-27 03:52
Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis,l-molecule ligands, analyzing conformational transitions in duplex DNA, measuring changes in helical repeat that accompany shifts in environmental conditions, and characterizing knotting and linking in duplex DNA.作者: vector 時間: 2025-3-27 05:24
Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers,eps are conducted in orthogonal directions with different concentrations of DNA intercalating agents. These compounds alter the overall shape of the DNA and, thereby, change the relative mobility of individual DNA topoisomers.作者: colony 時間: 2025-3-27 10:35
Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA,filter binding assay compares the ability of nicked and supercoiled forms of the circular DNA to compete for the binding of a .H-labeled nicked DNA to the topoisomerase where the enzyme–DNA complexes are quantitated by the retention of the labeled DNA on a nitrocellulose membrane.作者: 指派 時間: 2025-3-27 15:59 作者: dainty 時間: 2025-3-27 18:21
Studies in Computational Intelligenceoint using spindle morphology, chromosome condensation using fluorescently labeled chromosomal loci, and cell cycle progression by flow cytometry. Further characterization of this novel checkpoint is warranted so that we can further our understanding of the cell cycle, genomic stability, and the possibility of identifying novel drug targets.作者: habile 時間: 2025-3-27 22:03 作者: 搜集 時間: 2025-3-28 03:44 作者: FLEET 時間: 2025-3-28 08:59
Bahaaeddin Alareeni,Allam Hamdantterned photobleaching, LPk-GT2 cells were used to demonstrate the rapid dynamics of Topo IIα exchange in both interphase nuclei and mitotic chromosomes. These rapid dynamics are dependent on enzyme activity since ICRF159, a catalytic inhibitor of Topo IIα, slows dynamics significantly. The methods utilized in these studies are described herein.作者: Processes 時間: 2025-3-28 10:55 作者: 我沒有命令 時間: 2025-3-28 18:19
Assaying Topoisomerase II Checkpoints in Yeast,oint using spindle morphology, chromosome condensation using fluorescently labeled chromosomal loci, and cell cycle progression by flow cytometry. Further characterization of this novel checkpoint is warranted so that we can further our understanding of the cell cycle, genomic stability, and the possibility of identifying novel drug targets.作者: ULCER 時間: 2025-3-28 19:31 作者: catagen 時間: 2025-3-28 23:16 作者: NOCT 時間: 2025-3-29 05:35 作者: 反復無常 時間: 2025-3-29 10:35
1064-3745 f assays from cell-based methods and molecular analysis of t.Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular l作者: 腫塊 時間: 2025-3-29 11:54 作者: 攤位 時間: 2025-3-29 17:15 作者: Occupation 時間: 2025-3-29 22:34
Abdelkader Derbali,Kamel Naoui,Lamia Jamelchemotherapy regimens. Summarised here are the ways in which Topo II has been depleted or mutated in animal cells and the type of information gleaned. The cell lines generated are tabulated and represent a useful resource for further in vivo studies of Topo II function, one that we expect to grow in size and utility in the coming years.作者: condone 時間: 2025-3-30 02:11
Book 2009m a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In .DNA Topoisomerases: Methods and Protocols., experts in the field present a compendium of protocols that extends into the new areas of research that have been作者: 主動 時間: 2025-3-30 06:34 作者: strdulate 時間: 2025-3-30 12:02 作者: Platelet 時間: 2025-3-30 16:25 作者: 紡織品 時間: 2025-3-30 20:01 作者: 艱苦地移動 時間: 2025-3-30 21:33
Yu An,Qing Ye,Jiulin Guo,Ruihai Dongeps are conducted in orthogonal directions with different concentrations of DNA intercalating agents. These compounds alter the overall shape of the DNA and, thereby, change the relative mobility of individual DNA topoisomers.作者: 陳腐的人 時間: 2025-3-31 03:59
Lecture Notes in Computer Sciencefilter binding assay compares the ability of nicked and supercoiled forms of the circular DNA to compete for the binding of a .H-labeled nicked DNA to the topoisomerase where the enzyme–DNA complexes are quantitated by the retention of the labeled DNA on a nitrocellulose membrane.作者: Spartan 時間: 2025-3-31 07:46 作者: maudtin 時間: 2025-3-31 11:09 作者: Concerto 時間: 2025-3-31 15:18 作者: Sputum 時間: 2025-3-31 20:12
,Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase IIIα,s, which would have the effect of suppressing sister chromatid exchanges and loss of heterozygosity between homologous chromosomes. In this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro.作者: 聽寫 時間: 2025-3-31 22:04 作者: 不容置疑 時間: 2025-4-1 04:11 作者: prosthesis 時間: 2025-4-1 09:39 作者: CHYME 時間: 2025-4-1 13:56 作者: BANAL 時間: 2025-4-1 16:30 作者: COST 時間: 2025-4-1 22:15
Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II,I require the enzyme to generate a transient double-stranded break in the backbone of the double helix. To maintain genomic integrity during the cleavage event, topoisomerase II forms covalent bonds between active site tyrosyl residues and the newly generated 5′-DNA termini. In addition to the criti作者: Estrogen 時間: 2025-4-2 02:37
Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA,inactive mutant form of the enzyme. In the gel shift assay, the preference for binding to supercoiled plasmid DNA is detected in the presence of linear and nicked forms of the same DNA by a reduction in the mobility of the supercoiled plasmid during electrophoresis in agarose. The more quantitative 作者: 法律的瑕疵 時間: 2025-4-2 05:54
Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy T in regulating intracellular DNA supercoiling. Topoisomerase II (topo II) performs these DNA transactions by passing one segment of DNA through the other using a reversible, enzyme-bridged double-stranded DNA break. This cleavage/religation of the DNA backbone is coupled to the opening and closing o作者: Adornment 時間: 2025-4-2 09:18
Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases,rticularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.作者: 發(fā)酵劑 時間: 2025-4-2 14:41
