作者: Pseudoephedrine 時間: 2025-3-21 23:52 作者: Orgasm 時間: 2025-3-22 03:58
Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers,DNA molecules of totally different structures may have the same electrophoretic mobility. These DNA species can be resolved by two-dimensional (2-D) gel electrophoresis, which involves two successive operations carried out with one gel slab under different conditions and in orthogonal directions.作者: 愛國者 時間: 2025-3-22 08:05 作者: insincerity 時間: 2025-3-22 12:37
Purification of Supercoiled Plasmid DNA,he capability of generating both positive and negative DNA supercoils during the process of transcription and replication, respectively. These supercoils must be relaxed in order for transcription and replication to continue unaffected, as outlined in preceding chapters. The DNA topoisomerases play 作者: 一美元 時間: 2025-3-22 16:02
Purification and Use of DNA Minicircles with Different Linking Numbers,information in topologically independent domains consisting of one or more genes and of all the elements required in cis for their functioning. The elements that belong to the same functional unit are topologically linked: they depend on each other and cooperate.作者: 一美元 時間: 2025-3-22 18:22 作者: 博識 時間: 2025-3-23 00:27
Isolation of Knotted DNA from Coliphage P4,ssay for type II topoisomerase activity, even in crude extracts. Such assays have been useful in screening drugs that are suspected to inhibit type II topoisomerases. This chapter describes the isolation of knotted DNA from P4 phage. In the case of wild-type P4, such DNA molecules are double-strande作者: 煩躁的女人 時間: 2025-3-23 04:34 作者: interference 時間: 2025-3-23 05:44
Sedimentation Analysis of Bacterial Nucleoid Structure,f extracted nucleoids. Since the few DNA nicks that occur when nucleoids are isolated (.,.) are localized by the presence of 50–100 barriers to strand rotation (.,.), it is possible to recover chromosomal DNA in which most of each molecule is topologically constrained (.,.). Consequently, intracellu作者: 射手座 時間: 2025-3-23 09:53 作者: 價值在貶值 時間: 2025-3-23 16:09 作者: 媒介 時間: 2025-3-23 22:07 作者: mydriatic 時間: 2025-3-24 00:41 作者: SEVER 時間: 2025-3-24 04:50 作者: sebaceous-gland 時間: 2025-3-24 08:26
Overexpression and Purification of ,DNA Topoisomerase III,hanism of topoisomerases. The ability to overexpress and purify large quantities of these molecules has led to the elucidation of the crystal structures of the amino-terminal fragment of the GyrB subunit of . DNA gyrase (.), a large fragment of yeast topoisomerase II (topo II) (.), and of the first 作者: Cirrhosis 時間: 2025-3-24 13:12
Overexpression and Purification of Bacterial Topoisomerase IV,ins with a high degree of amino acid similarity to . and ., respectively. A new superhelical DNA relaxation activity could be demonstrated when extracts prepared from strains overproducing the . and . gene products were mixed together. ParC and ParE were subsequently purified (.,.), and it was demon作者: monopoly 時間: 2025-3-24 17:47 作者: acrobat 時間: 2025-3-24 20:31
Overexpression and Purification of DNA Topoisomerase I from Yeast,g camptothecin have been the subject of intense investigation (reviewedin (.-.). Much of this effort has focused on structure/function studies of wild-type and mutant forms of the enzyme, derived from a variety of sources (.,.-.). The budding yeast . has proven particularly amenable to the genetic m作者: labyrinth 時間: 2025-3-25 00:14 作者: 鴕鳥 時間: 2025-3-25 04:49 作者: 冰雹 時間: 2025-3-25 10:18 作者: 密切關(guān)系 時間: 2025-3-25 11:41
Purification of the Bacteriophage T4 Type II DNA Topoisomerase,al DNA gyrase (.,.). Indeed, the discovery of the T4 topoisomerase provided the first example of an ATP-dependent relaxing enzyme (.,.), and an understanding of the properties of the T4 enzyme rapidly led to the discovery of similar enzymes from eukaryotic cells (.-.).作者: PET-scan 時間: 2025-3-25 19:07
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260200.jpg作者: 帶來的感覺 時間: 2025-3-25 22:21 作者: 體貼 時間: 2025-3-26 02:26 作者: 情愛 時間: 2025-3-26 04:17 作者: 大吃大喝 時間: 2025-3-26 09:51 作者: absolve 時間: 2025-3-26 15:24
A Taxonomy of Explainable Bayesian Networkshe capability of generating both positive and negative DNA supercoils during the process of transcription and replication, respectively. These supercoils must be relaxed in order for transcription and replication to continue unaffected, as outlined in preceding chapters. The DNA topoisomerases play 作者: Scintillations 時間: 2025-3-26 20:41 作者: 逗留 時間: 2025-3-27 00:45 作者: enlist 時間: 2025-3-27 02:41 作者: 猛擊 時間: 2025-3-27 05:34 作者: Hallowed 時間: 2025-3-27 13:03
Lecture Notes in Networks and Systemsf extracted nucleoids. Since the few DNA nicks that occur when nucleoids are isolated (.,.) are localized by the presence of 50–100 barriers to strand rotation (.,.), it is possible to recover chromosomal DNA in which most of each molecule is topologically constrained (.,.). Consequently, intracellu作者: 許可 時間: 2025-3-27 14:11 作者: 兇兆 時間: 2025-3-27 20:16 作者: Occupation 時間: 2025-3-27 22:22 作者: 閑聊 時間: 2025-3-28 05:16
https://doi.org/10.1007/978-3-030-42726-9nzyme from . consists of two proteins, A and B (termed GyrA and GyrB), of molecular masses 97 and 90 kDa, respectively; the active enzyme is an A.B. complex. All DNA topoisomerases are able to relax negatively supercoiled DNA, but only gyrase can also catalyze the introduction of negative supercoils作者: amenity 時間: 2025-3-28 09:23
Mohamed Ihmeida,Muhammad Shahzadcommonly used biophysical methods. This is especially true for three-dimensional structure determination using X-ray crystallography or NMR. Structure–function analysis by site-directed mutagenesis requires that a large number of mutant enzymes be expressed and purified readily, so that their proper作者: gimmick 時間: 2025-3-28 13:01 作者: 走調(diào) 時間: 2025-3-28 15:58
Lecture Notes in Computer Scienceins with a high degree of amino acid similarity to . and ., respectively. A new superhelical DNA relaxation activity could be demonstrated when extracts prepared from strains overproducing the . and . gene products were mixed together. ParC and ParE were subsequently purified (.,.), and it was demon作者: 后天習得 時間: 2025-3-28 20:28 作者: Rotator-Cuff 時間: 2025-3-29 00:30 作者: aqueduct 時間: 2025-3-29 05:53 作者: photopsia 時間: 2025-3-29 08:51
Artificial Intelligence ResearchDNA molecules of totally different structures may have the same electrophoretic mobility. These DNA species can be resolved by two-dimensional (2-D) gel electrophoresis, which involves two successive operations carried out with one gel slab under different conditions and in orthogonal directions.作者: Catheter 時間: 2025-3-29 15:08
Huanyou Chai,Zhi Liu,Tianhui Hu,Qing Liinformation in topologically independent domains consisting of one or more genes and of all the elements required in cis for their functioning. The elements that belong to the same functional unit are topologically linked: they depend on each other and cooperate.作者: 混合物 時間: 2025-3-29 19:12 作者: CHURL 時間: 2025-3-29 20:56 作者: Offbeat 時間: 2025-3-30 01:10
Book 1999played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapte作者: 固定某物 時間: 2025-3-30 05:40 作者: anarchist 時間: 2025-3-30 11:29 作者: 都相信我的話 時間: 2025-3-30 16:28 作者: 不確定 時間: 2025-3-30 20:06
Sedimentation Analysis of Bacterial Nucleoid Structure,). Experiments of this type have contributed to the conclusions that (1) supercoiling is controlled in part by regulated expression of the gyrase and topoisomerase I genes, and (2) the overall level of supercoiling responds to growth environment (reviewed in [.]).作者: 殘酷的地方 時間: 2025-3-31 00:05
Overexpression and Purification of ,DNA Topoisomerase III,es, using topo I preparations purified from cells containing the gene encoding topo I (.) on a multicopy plasmid showed that topo I was capable of resolving plasmid DNA replication intermediates in vitro (.). It was not until the purification and characterization of topo III that it was realized tha作者: 安裝 時間: 2025-3-31 01:20
Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis,NA in agarose is also affected by the shape of the DNA, the pore size of the matrix (agarose concentration), temperature, the ionic strength of the electrophoresis buffer, the applied voltage/field strength, and the presence of intercalators (reviewed in .,.).作者: 閑聊 時間: 2025-3-31 06:33
Purification of Supercoiled Plasmid DNA,ils must be relaxed in order for transcription and replication to continue unaffected, as outlined in preceding chapters. The DNA topoisomerases play the central role in relaxing this supercoiling (.-.).作者: cipher 時間: 2025-3-31 09:25
Isolation of Kinetoplast DNA,NA has also proven a fascinating experimental subject in its own right. Studies of the unique structure, mechanism of replication, and genetic function of kDNA have led to numerous interesting discoveries (...-. for reviews).作者: Confess 時間: 2025-3-31 15:33
Isolation of Knotted DNA from Coliphage P4, topoisomerases. This chapter describes the isolation of knotted DNA from P4 phage. In the case of wild-type P4, such DNA molecules are double-stranded, hydrogen-bonded, knotted circles of 11,624 nucleotides.作者: ENACT 時間: 2025-3-31 21:15 作者: perpetual 時間: 2025-3-31 22:33
Overexpression and Purification of Bacterial Topoisomerase IV,ts prepared from strains overproducing the . and . gene products were mixed together. ParC and ParE were subsequently purified (.,.), and it was demonstrated that they formed a heterotetramer (.) with ATP-dependent, type II topoisomerase activity (.,.).作者: insightful 時間: 2025-4-1 02:18
Overexpression and Purification of DNA Topoisomerase I from Yeast,-type and mutant forms of the enzyme, derived from a variety of sources (.,.-.). The budding yeast . has proven particularly amenable to the genetic manipulations required to overexpress and purify wild-type and mutant forms of eukaryotic DNA topoisomerase I (.,.,.,.).作者: Admonish 時間: 2025-4-1 08:22
Iena Petronella Derks,Alta de Waalrally equivalent to the Holliday junction, an intermediate in homologous DNA recombination (.,.). Thus, an understanding of the thermodynamics and kinetics of cruciform formation may illuminate a number of processes in nucleic acid metabolism.作者: Overdose 時間: 2025-4-1 12:49
