作者: FIN 時(shí)間: 2025-3-21 23:51 作者: Genistein 時(shí)間: 2025-3-22 01:22 作者: concentrate 時(shí)間: 2025-3-22 05:16
Thokozile Manaka,Terence van Zyl,Deepak Kar complete double-helical turn). The end result of such a nicking-rejoining process in the presence of a DNA-unwinding agent is the formation of a relaxed underwound DNA molecule with a deficient linking number (the number of times one strand goes around the other in a duplex ring). The relaxed under作者: Generator 時(shí)間: 2025-3-22 12:34 作者: menopause 時(shí)間: 2025-3-22 13:13 作者: menopause 時(shí)間: 2025-3-22 20:08
Book 2001played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapte作者: 接觸 時(shí)間: 2025-3-22 23:56 作者: Inferior 時(shí)間: 2025-3-23 05:05
Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation,nked to each of the generated 5′-ends. Several studies have utilized the detergent method to define the sites of interaction between DNA and topoisomerase I and II in vitro as well as in vivo (.,.). One of the major problems in these studies has been ascribed to the involvement of SDS in trapping cl作者: blithe 時(shí)間: 2025-3-23 08:31 作者: NOTCH 時(shí)間: 2025-3-23 11:41
DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I, complete double-helical turn). The end result of such a nicking-rejoining process in the presence of a DNA-unwinding agent is the formation of a relaxed underwound DNA molecule with a deficient linking number (the number of times one strand goes around the other in a duplex ring). The relaxed under作者: opportune 時(shí)間: 2025-3-23 16:29
,Drug—DNA Interactions,y correlate with antitumor activity; however, modulation of topoisomerase . activity was shown to coincide with biological activity of these agents (.). Interactions of ligands with DNA are being explored using a variety of physical and biochemical methods in an effort to determine the chemical and 作者: 不法行為 時(shí)間: 2025-3-23 19:09
Drug Toxicity in , Cells Expressing Human Topoisomerase I,single 5’-phosphodiester intermediate (.–.). The etopoI enzyme is very efficient at relaxing highly negatively supercoiled DNA and shows progressively decreasing activity as the substrate DNA becomes more relaxed (.,.). The htopoI enzyme relaxes both negatively and positively supercoiled DNA to comp作者: 燒烤 時(shí)間: 2025-3-24 01:09 作者: refine 時(shí)間: 2025-3-24 05:11 作者: 憂傷 時(shí)間: 2025-3-24 07:25 作者: 得罪人 時(shí)間: 2025-3-24 12:19
Habiba Drias,Farouk Yalaoui,Allel HadjaliIn “inventing” reverse gyrase, the living world has built a unique and remarkably sophisticated enzyme that is more than a simple topoisomerase. Reverse gyrase possesses the unique ability to catalyze the production of positive supercoils in a closedcircular DNA at the expense of ATP (.); . . . for review.作者: VEST 時(shí)間: 2025-3-24 16:49 作者: cumulative 時(shí)間: 2025-3-24 20:03
DNA Topoisomerase Protocols978-1-59259-057-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Electrolysis 時(shí)間: 2025-3-25 00:06 作者: foliage 時(shí)間: 2025-3-25 06:46 作者: FLORA 時(shí)間: 2025-3-25 08:41 作者: 不持續(xù)就爆 時(shí)間: 2025-3-25 13:21 作者: maculated 時(shí)間: 2025-3-25 16:52
Habiba Drias,Farouk Yalaoui,Allel Hadjali among the topoisomerases in promoting negative supercoiling of DNA (.,.). A variety of studies have shown that gyrase is essential for bacterial growth with roles in DNA replication, transcription, and recombination. Moreover, in combination with the relaxing activity of DNA topoisomerase I, gyrase作者: 施加 時(shí)間: 2025-3-25 21:43
https://doi.org/10.1007/978-3-031-14489-9ic functions (.–.). ATP binding is necessary to close the protein clamp (.,.) and trigger DNA strand passage (.,.), whereas hydrolysis is necessary for topoisomerase II recycling (.,.). Beyond the the critical roles played by ATP in the catalytic cycle of the enzyme, interactions between type II top作者: Omnipotent 時(shí)間: 2025-3-26 02:09 作者: 拱墻 時(shí)間: 2025-3-26 07:30 作者: 大炮 時(shí)間: 2025-3-26 08:37 作者: overrule 時(shí)間: 2025-3-26 16:12 作者: 遍及 時(shí)間: 2025-3-26 19:05
Paraconsistent Rough Set Algebras one DNA strand and allows the passage of single-stranded DNA through the generated nick, whereas topoisomerase II introduces a transient double-stranded break and permits passage of another double helix through the gap (.–.). Owing to the physiological importance of the topoisomerase-mediated cleav作者: surmount 時(shí)間: 2025-3-26 21:16
Armand Graaff,Danie Smit,Sunet Eybersdiester bonds. Strand cleavage is not the result of phosphodiester hydrolysis, but the result of transesterification of an active-site tyrosine, creating a 3′-DNA covalent intermediate and a 5′-terminus (5′-OH). Strand ligation occurs during a second transesterification event when the 5′-OH displace作者: vascular 時(shí)間: 2025-3-27 02:41 作者: Individual 時(shí)間: 2025-3-27 07:21 作者: 誹謗 時(shí)間: 2025-3-27 12:13
Thokozile Manaka,Terence van Zyl,Deepak Kar effects or toxicity. DNA unwinding results in a lengthening of the double helix. This increase in length can be detected by physicochemical (such as electric dichroism) or hydrodynamic methods (such as viscosity and sedimentation measurements) using short segments of linear DNA. These methods mostl作者: Semblance 時(shí)間: 2025-3-27 13:46
https://doi.org/10.1007/978-3-030-95070-5ecades; however, critical information linking the physical-chemical properties associated with these complexes with their biological effectiveness remains unclear. Significant progress has been made toward unraveling the structural and dynamic properties of many ligand-DNA complexes, which has provi作者: 美麗的寫 時(shí)間: 2025-3-27 18:56 作者: 情愛 時(shí)間: 2025-3-28 01:52
Edgar Jembere,Aurona J. Gerber,Anban Pillayxert their lethality on . by inhibiting the normal function of the DNA gyrase (a bacterial topoisomerase II) in this organism. Mutations around a limited region of the DNA gyrase A subunit (residues Ala67-Gln106) have been shown to confer resistance to these agents in . (.). This has also been docum作者: Gleason-score 時(shí)間: 2025-3-28 05:32 作者: 艱苦地移動(dòng) 時(shí)間: 2025-3-28 09:01 作者: 寵愛 時(shí)間: 2025-3-28 10:56 作者: hauteur 時(shí)間: 2025-3-28 17:29
Book 2001ze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinical作者: 小臼 時(shí)間: 2025-3-28 22:03
https://doi.org/10.1007/978-3-031-14489-9gyrase function specifically by inhibiting ATP hydrolysis (.–.). In addition, several anticancer drugs that act by enhancing topoisomerase II-mediated DNA cleavage also impair interactions between the enzyme and ATP (.).作者: VAN 時(shí)間: 2025-3-29 02:36
Xuecheng Hou,Ning Kang,Yixiang Chenhe filter, or found in filtrates and washes. Because protein-adsorbing materials can differ in their efficiency, capacity, and flow characteristics, filters from the same lot should be used in serial experiments, and the optimal filtration conditions need to be empirically determined in each case.作者: Geyser 時(shí)間: 2025-3-29 05:40 作者: 金哥占卜者 時(shí)間: 2025-3-29 09:23
,Filter Binding Assays for Topoisomerase—DNA Complexes,he filter, or found in filtrates and washes. Because protein-adsorbing materials can differ in their efficiency, capacity, and flow characteristics, filters from the same lot should be used in serial experiments, and the optimal filtration conditions need to be empirically determined in each case.作者: 火光在搖曳 時(shí)間: 2025-3-29 14:07
Habiba Drias,Farouk Yalaoui,Allel Hadjalihe directional crossing of a DNA duplex through a transient enzyme-bridged double-strand break in a 120–150 bp segment of DNA wrapped on the enzyme (.–.). This process changes the linking number of DNA in steps of two, and together with an ability to form and resolve DNA knots and catenanes, establishes gyrase as a type II topoisomerase.作者: 植物茂盛 時(shí)間: 2025-3-29 18:20 作者: DAFT 時(shí)間: 2025-3-29 22:08
Plasmid DNA Supercoiling by DNA Gyrase,he directional crossing of a DNA duplex through a transient enzyme-bridged double-strand break in a 120–150 bp segment of DNA wrapped on the enzyme (.–.). This process changes the linking number of DNA in steps of two, and together with an ability to form and resolve DNA knots and catenanes, establishes gyrase as a type II topoisomerase.作者: ingrate 時(shí)間: 2025-3-30 03:32 作者: 諂媚于性 時(shí)間: 2025-3-30 04:53 作者: Neutropenia 時(shí)間: 2025-3-30 11:23 作者: agitate 時(shí)間: 2025-3-30 15:39
Armand Graaff,Danie Smit,Sunet Eybersing a 3′-DNA covalent intermediate and a 5′-terminus (5′-OH). Strand ligation occurs during a second transesterification event when the 5′-OH displaces the enzyme (. .). A change in DNA linking number results when strand unwinding occurs between these cleavage and ligation reactions.作者: Irksome 時(shí)間: 2025-3-30 19:21
ICE Bioassay,the cleavage site. This ability of topoisomerases to catalyze concerted breaking and rejoining of DNA strands makes them biologically important enzymes that are involved in crucial cellular processes (.–.).作者: 驚惶 時(shí)間: 2025-3-30 23:41
Quinolone Interactions with DNA and DNA Gyrase,ocess of drug design by furthering our understanding of the established structure-activity relationship of that class of compounds. The method has been best exemplified by the investigations of mode of action of quinolone antibacterials that target the bacteria-specific type II DNA topoisomerase, DNA gyrase (reviewed in . .–.).作者: cathartic 時(shí)間: 2025-3-31 04:55
Bactericidal Assays for Fluoroquinolones,ented in many other organisms, indicating that DNA gyrase is a universal target for these drugs. This relationship has been somewhat complicated recently by the observation that in ., mutations in another type II enzyme, topoisomerase IV, also confer resistance to fluoroquinolones (.).作者: 白楊魚 時(shí)間: 2025-3-31 07:05 作者: 溝通 時(shí)間: 2025-3-31 10:30
,Synthesis and Use of DNA Containing a 5′-Bridging Phosphorothioate as a Suicide Substrate for Type ing a 3′-DNA covalent intermediate and a 5′-terminus (5′-OH). Strand ligation occurs during a second transesterification event when the 5′-OH displaces the enzyme (. .). A change in DNA linking number results when strand unwinding occurs between these cleavage and ligation reactions.作者: 靦腆 時(shí)間: 2025-3-31 14:48
Fawwaz Mohammed,Lisa F. Seymourthe cleavage site. This ability of topoisomerases to catalyze concerted breaking and rejoining of DNA strands makes them biologically important enzymes that are involved in crucial cellular processes (.–.).作者: 嚴(yán)厲批評(píng) 時(shí)間: 2025-3-31 19:31
https://doi.org/10.1007/978-3-030-95070-5ocess of drug design by furthering our understanding of the established structure-activity relationship of that class of compounds. The method has been best exemplified by the investigations of mode of action of quinolone antibacterials that target the bacteria-specific type II DNA topoisomerase, DNA gyrase (reviewed in . .–.).作者: Occlusion 時(shí)間: 2025-3-31 22:07 作者: 使苦惱 時(shí)間: 2025-4-1 02:22 作者: Stagger 時(shí)間: 2025-4-1 06:47 作者: fringe 時(shí)間: 2025-4-1 11:04 作者: Condense 時(shí)間: 2025-4-1 16:43
Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography,ic functions (.–.). ATP binding is necessary to close the protein clamp (.,.) and trigger DNA strand passage (.,.), whereas hydrolysis is necessary for topoisomerase II recycling (.,.). Beyond the the critical roles played by ATP in the catalytic cycle of the enzyme, interactions between type II top
