標(biāo)題: Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D [打印本頁(yè)] 作者: informed 時(shí)間: 2025-3-21 16:06
書(shū)目名稱DNA Replication影響因子(影響力)
作者: Platelet 時(shí)間: 2025-3-21 22:35
Microscopy Techniques to Examine DNA Replication in Fission Yeast,lenging. Choosing the appropriate method depends upon the parameters of the experiment, the nature of the molecules to be observed, and the hypothesis to be tested. In this chapter, we review three broad types of visualization: whole-cell fluorescence or immunofluorescence, which is useful for quest作者: Epidural-Space 時(shí)間: 2025-3-22 03:38
High-Resolution Analysis of Mammalian DNA Replication Units,e course of S-phase, semiconservative DNA synthesis is sequentially initiated and performed at thousands of discrete patches of the DNA helix termed replicons. At any given moment of S-phase, multiple replicons are active in parallel in different parts of the genome. In the last decades, tools and m作者: 加花粗鄙人 時(shí)間: 2025-3-22 07:46
Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing,sites distributed along mammalian chromosomes, yet a given cell uses only a subset of these origins due to inefficient origin activation and regulation by developmental or environmental cues. An impractical consequence of cell-to-cell variations in origin firing is that population-based techniques d作者: insidious 時(shí)間: 2025-3-22 09:45 作者: MOTIF 時(shí)間: 2025-3-22 16:06 作者: MOTIF 時(shí)間: 2025-3-22 18:46 作者: 串通 時(shí)間: 2025-3-23 01:00
Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Excnd accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analys作者: 把…比做 時(shí)間: 2025-3-23 03:00
Measuring Ribonucleotide Incorporation into DNA In Vitro and In Vivo,nsequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.作者: 單調(diào)性 時(shí)間: 2025-3-23 06:02 作者: Ingenuity 時(shí)間: 2025-3-23 13:44 作者: Chemotherapy 時(shí)間: 2025-3-23 15:40 作者: Glaci冰 時(shí)間: 2025-3-23 21:14 作者: 松緊帶 時(shí)間: 2025-3-24 00:21 作者: Rebate 時(shí)間: 2025-3-24 03:01 作者: Torrid 時(shí)間: 2025-3-24 09:46 作者: 怒目而視 時(shí)間: 2025-3-24 13:56 作者: septicemia 時(shí)間: 2025-3-24 16:15 作者: 音樂(lè)等 時(shí)間: 2025-3-24 21:42
978-1-4939-5591-6Springer Science+Business Media New York 2015作者: fastness 時(shí)間: 2025-3-25 00:55
Sonya Vengrova,Jacob DalgaardIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 善于騙人 時(shí)間: 2025-3-25 03:55
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260188.jpg作者: 減至最低 時(shí)間: 2025-3-25 09:56
https://doi.org/10.1007/978-3-642-73739-8ation. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.作者: 全部逛商店 時(shí)間: 2025-3-25 14:53
A Chinese Model of Education in New Zealandlenging. Choosing the appropriate method depends upon the parameters of the experiment, the nature of the molecules to be observed, and the hypothesis to be tested. In this chapter, we review three broad types of visualization: whole-cell fluorescence or immunofluorescence, which is useful for quest作者: faultfinder 時(shí)間: 2025-3-25 16:55
https://doi.org/10.1007/978-981-10-0330-1e course of S-phase, semiconservative DNA synthesis is sequentially initiated and performed at thousands of discrete patches of the DNA helix termed replicons. At any given moment of S-phase, multiple replicons are active in parallel in different parts of the genome. In the last decades, tools and m作者: insidious 時(shí)間: 2025-3-25 23:29 作者: indubitable 時(shí)間: 2025-3-26 03:58 作者: 疏遠(yuǎn)天際 時(shí)間: 2025-3-26 08:09 作者: VAN 時(shí)間: 2025-3-26 11:23 作者: habitat 時(shí)間: 2025-3-26 13:42 作者: 一窩小鳥(niǎo) 時(shí)間: 2025-3-26 18:41 作者: SLUMP 時(shí)間: 2025-3-26 22:20 作者: 全神貫注于 時(shí)間: 2025-3-27 02:25 作者: Decrepit 時(shí)間: 2025-3-27 06:17
Peter J. Peverelli,Jiwen Song,Wei Siunters a variety of obstacles including DNA damage/adducts, secondary structures, and programmed fork-blocking sites, which are all difficult to replicate. The replication fork also collides with the transcription machinery, which shares the template DNA with the replisome complex. Under these condi作者: Eosinophils 時(shí)間: 2025-3-27 09:59
Environmental Politics and Theoryssential and nonessential protein factors in a spatially and temporally coordinated manner. Much of what is known about the enzymes and mechanisms of chromosome replication has come from analysis of simple microbial model systems, such as yeast and archaea. Archaea possess a highly simplified eukary作者: octogenarian 時(shí)間: 2025-3-27 14:55
Environmental Politics and Theoryolecule techniques have enabled the observation of dynamic behavior of individual replisome components and of the replisome as a whole, aspects that previously often have been obscured by ensemble averaging in more classical solution-phase biochemical experiments. To improve robustness and reproduci作者: Metastasis 時(shí)間: 2025-3-27 19:43
Chinese Environmental Governance site-specific replication fork arrest. Here we provide protocols for the use of the fission yeast . replication fork barrier. The . barrier is a directional, or polar, replication fork barrier that evolved to ensure directional replication of the fission yeast mating-type locus. We have moved the 8作者: GROVE 時(shí)間: 2025-3-28 00:13 作者: Seminar 時(shí)間: 2025-3-28 02:46 作者: linear 時(shí)間: 2025-3-28 08:26 作者: poliosis 時(shí)間: 2025-3-28 13:09
https://doi.org/10.1007/978-3-642-73739-8ation. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.作者: paltry 時(shí)間: 2025-3-28 15:36
Chinese Entrepreneurs in Societynsequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.作者: Canopy 時(shí)間: 2025-3-28 19:35 作者: Inscrutable 時(shí)間: 2025-3-29 00:52 作者: Aspiration 時(shí)間: 2025-3-29 05:27 作者: 偽書(shū) 時(shí)間: 2025-3-29 10:37
https://doi.org/10.1057/9781137492913, the study of DNA replication with multicolour flow analysis has lagged behind its use in mammalian cells. We present basic and advanced protocols for analysis of DNA replication in fission yeast by flow cytometry including whole cell, nuclear “ghosts,” two-color imaging with BrdU, and estimates of DNA synthesis using EdU.作者: NAV 時(shí)間: 2025-3-29 13:23 作者: hemophilia 時(shí)間: 2025-3-29 18:16 作者: habile 時(shí)間: 2025-3-29 22:11 作者: Vulnerary 時(shí)間: 2025-3-30 01:35 作者: 語(yǔ)源學(xué) 時(shí)間: 2025-3-30 06:15 作者: FICE 時(shí)間: 2025-3-30 10:34
Chinese Entrepreneurs in Societyrification of fragments and preparation of strand-specific sequencing libraries. Deep sequencing of Okazaki fragments generates a comprehensive, genomic map of DNA synthesis, starting from a single asynchronous culture. Altogether this approach represents a tractable system to investigate key aspects of DNA replication and chromatin assembly.作者: HACK 時(shí)間: 2025-3-30 13:52 作者: Rustproof 時(shí)間: 2025-3-30 17:00 作者: BUDGE 時(shí)間: 2025-3-30 21:17 作者: outer-ear 時(shí)間: 2025-3-31 04:32 作者: 使入迷 時(shí)間: 2025-3-31 05:08
Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast,ine monophosphate, which can be used by the cell. This chapter describes the labeling of fission yeast, ., with the thymidine analog BrdU in order to identify sites and determine kinetics of DNA replication.作者: Control-Group 時(shí)間: 2025-3-31 09:17 作者: SOW 時(shí)間: 2025-3-31 13:49
Book 2015Latest editionfalls..Authoritative and cutting-edge, .DNA Replication: Methods and Protocols, Second Edition .provides a collections of methods intended for newcomers to this research field and for established laboratories..作者: 征稅 時(shí)間: 2025-3-31 19:46
https://doi.org/10.1057/9781137492913 This allows quantitative measurements of several salient features of chromosome replication dynamics, such as fork velocity, fork asymmetry, inter-origin distances, and global instant fork density. In this chapter we describe how to obtain this information from asynchronous cultures of mammalian cells.作者: inflate 時(shí)間: 2025-3-31 22:14 作者: RLS898 時(shí)間: 2025-4-1 02:19
Chinese Environmental Governancehich we regulate the activity of the barrier, how to synchronize cells for analysis of replication intermediates by 2D gel electrophoresis, and the use of a replication slippage assay to measure fork fidelity.作者: extemporaneous 時(shí)間: 2025-4-1 10:02
Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing, This allows quantitative measurements of several salient features of chromosome replication dynamics, such as fork velocity, fork asymmetry, inter-origin distances, and global instant fork density. In this chapter we describe how to obtain this information from asynchronous cultures of mammalian cells.作者: 松軟 時(shí)間: 2025-4-1 12:44
Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors,ated at chromosomal regions that are difficult to replicate. In this chapter, we describe a chromatin immunoprecipitation method to locate proteins required for DNA repair during DNA replication in the fission yeast .. This method can also easily be adapted to study replisome components or chromatin-associated factors.
