標題: Titlebook: DNA Repair Protocols; Lotte Bjergb?k Book 2012Latest edition Springer Science+Business Media New York 2012 Arabidopsis cell extracts.DNA r [打印本頁] 作者: Herbaceous 時間: 2025-3-21 16:20
書目名稱DNA Repair Protocols影響因子(影響力)
書目名稱DNA Repair Protocols影響因子(影響力)學科排名
書目名稱DNA Repair Protocols網(wǎng)絡公開度
書目名稱DNA Repair Protocols網(wǎng)絡公開度學科排名
書目名稱DNA Repair Protocols被引頻次
書目名稱DNA Repair Protocols被引頻次學科排名
書目名稱DNA Repair Protocols年度引用
書目名稱DNA Repair Protocols年度引用學科排名
書目名稱DNA Repair Protocols讀者反饋
書目名稱DNA Repair Protocols讀者反饋學科排名
作者: FLAGR 時間: 2025-3-21 23:29 作者: xanthelasma 時間: 2025-3-22 03:29 作者: 偽書 時間: 2025-3-22 08:35
Establishment of the DNA Repair-Defective Mutants in DT40 Cellsding efficient gene targeting and ease of chromosome manipulation. Although the genetic approach using the RNA interference technique has become the standard method particularly in human cells, DT40 still remains a powerful tool to investigate the regulation and function of genes and proteins in a v作者: 拱墻 時間: 2025-3-22 11:21 作者: 抱負 時間: 2025-3-22 16:27
The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repairs technique, a small number of cells suspended in a thin agarose gel on a microscope slide is lysed, electrophoresed, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased migration of chromosomal DNA from the nucleus towards the anode, which resembles the作者: 抱負 時間: 2025-3-22 18:56 作者: archetype 時間: 2025-3-23 00:36
Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assayive effects of chemicals. The main advantage over the comet assay using cells of higher organisms is the genetic tractability and ease of cultivation of yeast. A drawback is the lower DNA content of the cells as well as the need for cell wall digestion prior to electrophoresis. Here, we describe in 作者: Misnomer 時間: 2025-3-23 03:11
Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitativ nonmammalian species such as . (nematodes), . (fruit flies), and two species of fish (. and .). Since its development in the early 1990s (Kalinowski et al., Nucleic Acids Res 20:3485–3494, 1992; Salazar and Van Houten, Mutat Res 385:139–149, 1997; Yakes and Van Houten, Proc Natl Acad Sci USA 94:514作者: 包裹 時間: 2025-3-23 07:50
In Vitro DNA Mismatch Repair in Human Cellstenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and t作者: 運動性 時間: 2025-3-23 13:33 作者: SHRIK 時間: 2025-3-23 15:01 作者: 使饑餓 時間: 2025-3-23 21:38 作者: BOOM 時間: 2025-3-23 23:35
Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCRs, by the transcriptional status of the locus and by proteins associated with the DNA. The only method currently available to allow precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated polymerase chain reaction (LM-PCR) technique. We provide an update on technical deta作者: Verify 時間: 2025-3-24 03:45
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cecal and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.作者: 問到了燒瓶 時間: 2025-3-24 06:48
Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extractson. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA tem作者: 針葉 時間: 2025-3-24 14:04 作者: intolerance 時間: 2025-3-24 17:47 作者: 小官 時間: 2025-3-24 21:49
,Infestation with Anoplura—Lice,ing, apoptosis, and DNA damage responses. Many genetic tools and tricks have been developed in . including knock down of gene expression via RNA interference (RNAi). In . RNAi can effectively be administrated via feeding the nematodes bacteria expressing double-stranded RNA targeting the gene of int作者: flavonoids 時間: 2025-3-25 01:58
Patrick Orth MD,Henning Madry MDding efficient gene targeting and ease of chromosome manipulation. Although the genetic approach using the RNA interference technique has become the standard method particularly in human cells, DT40 still remains a powerful tool to investigate the regulation and function of genes and proteins in a v作者: concubine 時間: 2025-3-25 05:14 作者: Bravado 時間: 2025-3-25 11:32
https://doi.org/10.1007/978-3-030-79423-1s technique, a small number of cells suspended in a thin agarose gel on a microscope slide is lysed, electrophoresed, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased migration of chromosomal DNA from the nucleus towards the anode, which resembles the作者: doxazosin 時間: 2025-3-25 13:48
https://doi.org/10.1007/978-3-030-79423-1l-established methods are combined, the Comet assay (single cell gel electrophoresis) and fluorescence in situ hybridization (FISH). The Comet assay is the method of choice for the detection of DNA damage. With the alkaline version the influence of specific substances such as water pollutants or ing作者: 一罵死割除 時間: 2025-3-25 16:04
Endoscopic Trigger Finger Releaseive effects of chemicals. The main advantage over the comet assay using cells of higher organisms is the genetic tractability and ease of cultivation of yeast. A drawback is the lower DNA content of the cells as well as the need for cell wall digestion prior to electrophoresis. Here, we describe in 作者: 清晰 時間: 2025-3-25 22:04
Wrist Portals and Arthroscopic Anatomy nonmammalian species such as . (nematodes), . (fruit flies), and two species of fish (. and .). Since its development in the early 1990s (Kalinowski et al., Nucleic Acids Res 20:3485–3494, 1992; Salazar and Van Houten, Mutat Res 385:139–149, 1997; Yakes and Van Houten, Proc Natl Acad Sci USA 94:514作者: 帳單 時間: 2025-3-26 01:27
Radio-Carpal and Midcarpal Arthroscopytenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and t作者: Militia 時間: 2025-3-26 07:45
Arthroscopic Anatomy of Shoulderrlies hereditary nonpolyposis colorectal cancer and some sporadic cancers. Because of remarkably high conservation of the mismatch repair machinery between the budding yeast (.) and humans, the study of mismatch repair in yeast has provided tremendous insights into the mechanisms of this repair path作者: 特別容易碎 時間: 2025-3-26 08:49
Robert U. Hartzler,Jose M. Gutierrez-Naranjo mechanisms of UVR-induced DNA damage may help prevent skin cancer and this may be achieved using methods to quantify DNA damage. The immuno-slot blot (ISB) method is routinely used for detection and quantification of any heat- and alkali-stable DNA adducts for which a sufficiently specific monoclon作者: 適宜 時間: 2025-3-26 13:51 作者: 寬敞 時間: 2025-3-26 20:45 作者: 增減字母法 時間: 2025-3-27 00:35 作者: etiquette 時間: 2025-3-27 02:34 作者: Irrepressible 時間: 2025-3-27 07:25
https://doi.org/10.1007/978-1-61779-998-3Arabidopsis cell extracts; DNA repair; RNA; Saccharomyces cerevisiae; chromosomal DNA; electrophoretic mo作者: Forsake 時間: 2025-3-27 10:49
978-1-4939-5954-9Springer Science+Business Media New York 2012作者: 保全 時間: 2025-3-27 16:45 作者: NEG 時間: 2025-3-27 21:29 作者: archaeology 時間: 2025-3-28 01:20
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cecal and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.作者: 過時 時間: 2025-3-28 03:56
DNA Repair Protocols978-1-61779-998-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 宣稱 時間: 2025-3-28 09:12
Pathologic Changes in the Joint Cavitycal and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.作者: 實施生效 時間: 2025-3-28 13:53
Book 2012Latest editionrence, advanced proteomics and microscopy as well as high throughput screens. The third?edition of .DNA Repair Protocols .covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mamma作者: 火光在搖曳 時間: 2025-3-28 18:23 作者: jaundiced 時間: 2025-3-28 19:39 作者: 針葉類的樹 時間: 2025-3-29 01:55 作者: CAB 時間: 2025-3-29 06:27 作者: HUMP 時間: 2025-3-29 10:57
Patrick Orth MD,Henning Madry MD homologous recombination and translesion synthesis activities using activation-induced deaminase (AID)-induced diversification of the immunoglobulin locus. In this chapter, we would describe a detailed protocol for gene disruption experiments in DT40 cells.作者: malign 時間: 2025-3-29 14:08
Zhao Lilian,Li Yanjin,Fu Chuyingd organ cultures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.作者: 暴露他抗議 時間: 2025-3-29 17:21 作者: Scintillations 時間: 2025-3-29 20:19
Quantification of DNA Photoproducts in Mammalian Cell DNA Using Radioimmunoassayd organ cultures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.作者: temperate 時間: 2025-3-30 02:35
In Vitro DNA Mismatch Repair in Human Cellss required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and the repair assay. In this chapter, we describe the detailed methods for this functional in vitro assay.作者: contradict 時間: 2025-3-30 06:19
Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCRapping of DNA lesions in mammalian cells is the ligation-mediated polymerase chain reaction (LM-PCR) technique. We provide an update on technical details of LM-PCR. LM-PCR can be used, for example, for mapping of ultraviolet (UV) light-induced DNA photoproducts such as cyclobutane pyrimidine dimers.作者: 制度 時間: 2025-3-30 11:05 作者: NAG 時間: 2025-3-30 15:41
Chagas Disease (American Trypanosomiasis)he etiologies of cancer, aging, and neurodegeneration and there is a great deal of interest in this venture. Thus, understanding of DNA processing is now a central field in molecular and cellular biology, and the field is still growing.作者: 人類 時間: 2025-3-30 17:52
https://doi.org/10.1007/978-3-319-13884-8e describe a high-throughput method for the identification of genes essential for cell survival following DNA damage by using a cell-based assay to measure viability in combination with an RNA interference-based genome-wide screening experiment.作者: EXULT 時間: 2025-3-30 23:52
,Infestation with Anoplura—Lice,erest. Several commercial . RNAi libraries are available and hence gene inactivation using RNAi can relatively easily be performed in a genome-wide fashion. In this chapter we give a protocol for using genome-wide RNAi screening to identify genes involved with the response to genotoxic stress.作者: membrane 時間: 2025-3-31 04:46 作者: 等級的上升 時間: 2025-3-31 05:10 作者: Decimate 時間: 2025-3-31 09:54
Arthroscopic Evaluation of Elbow Instabilityformation, one can apply variations of the EMSA, which include the reverse EMSA to detect binding of .S-labeled protein to damaged DNA, and the antibody supershift assay to detect the presence of a specific protein in the protein–DNA complex.
