標(biāo)題: Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan [打印本頁] 作者: Callow 時間: 2025-3-21 17:21
書目名稱DNA Repair Protocols影響因子(影響力)
書目名稱DNA Repair Protocols影響因子(影響力)學(xué)科排名
書目名稱DNA Repair Protocols網(wǎng)絡(luò)公開度
書目名稱DNA Repair Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱DNA Repair Protocols被引頻次
書目名稱DNA Repair Protocols被引頻次學(xué)科排名
書目名稱DNA Repair Protocols年度引用
書目名稱DNA Repair Protocols年度引用學(xué)科排名
書目名稱DNA Repair Protocols讀者反饋
書目名稱DNA Repair Protocols讀者反饋學(xué)科排名
作者: Aspiration 時間: 2025-3-21 23:22
Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes,ng ultraviolet (UV) light-induced DNA lesions, may result in the rare, recessively inherited autosomal syndromes xeroderma pigmentosum (XP), Cockayne syndrome (CS), or trichothiodystrophy (TTD). Classical approaches such as somatic cell fusions or microinjection assays have formalized the genetic co作者: 全國性 時間: 2025-3-22 02:17 作者: PAEAN 時間: 2025-3-22 07:34
Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation,ced genomic instability. Perhaps the best characterized is the dynamic production of chromosomal rearrangements in some clonally expanded cells surviving irradiation. In this chapter we provide the protocols for irradiation, cell culture, chromosome analysis, and characterization of the status of ge作者: DEFER 時間: 2025-3-22 09:33 作者: 規(guī)范要多 時間: 2025-3-22 15:31 作者: 規(guī)范要多 時間: 2025-3-22 17:31
Cytometric Assessment of Histone H2AX Phosphorylation,e. Immunocytochemical detection of phosphorylated H2AX (denoted as γH2AX) thus provides a marker of DSBs. The method presented in this chapter describes the detection of γH2AX for revealing the presence of DSBs, combined with differential staining of cellular DNA for revealing the cell cycle phase. 作者: 猛擊 時間: 2025-3-22 22:02
Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Celde of cell death. This chapter describes methods to label . DNA strand breaks with fluorochromes for detection by flow or laser scanning cytometry. By staining DNA with a fluorochrome of another color, cellular DNA content is measured concurrently and the bivariate analysis of such a data reveals DN作者: 芳香一點 時間: 2025-3-23 04:09
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA,nd have helped to characterize several components including DNA-PKcs, Ku, DNA ligase IV, and XRCC4. There is evidence, however, that additional factors involved in NHEJ remain to be characterized. The biochemical characterization of NHEJ in higher eukaryotes has benefited significantly from in vitro作者: 酷熱 時間: 2025-3-23 07:42
Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR andask any site-dependent variations in breakage and repair rate or fidelity. We present here a system for analyzing the fate of documented DNA breaks, using the . gene as an example, through application of ligation-mediated PCR. Here, a simple asymmetric double-stranded DNA adapter molecule is ligated作者: ATOPY 時間: 2025-3-23 12:21 作者: GLOSS 時間: 2025-3-23 16:48
Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants,seases. Gene targeting approaches are also useful for studying the mechanisms of homologous recombination. We have developed gene targeting methods that we have specifically used to investigate the mechanisms of recombination in cultured mammalian cells. In this chapter, we describe the generation o作者: 消滅 時間: 2025-3-23 20:40 作者: painkillers 時間: 2025-3-23 23:45
Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian ication of long DNA targets. This assay has been extensively used to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins, and has proved particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA (mtDNA) damage. QPCR can be use作者: forebear 時間: 2025-3-24 04:03
Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR, the genome is modulated by the DNA sequence, by DNA methylation patterns, by the transcriptional status of the locus, and by chromatin proteins associated with the DNA. The only method currently available to allow a precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated作者: 商談 時間: 2025-3-24 07:52
Immunochemical Detection of UV-Induced DNA Damage and Repair,eous genetic damage has developed. This, in turn, has increased interest in the cellular mechanisms responsible for tumorigenesis, and the need to develop experimental methodologies to investigate these mechanisms. DNA represents a most important cellular target for ultraviolet radiation (UVR), lead作者: 極端的正確性 時間: 2025-3-24 11:20
A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts,t light (UV-C)—pyrimidine-pyrimidone 6-4 photoproducts ([6-4]PPs) and cyclobutane pyrimidine dimers (CPDs). DNA extracted from irradiated cells is applied to a nitrocellulose dot-blot and quantified using an enzyme-conjugated secondary antibody and a color assay. Though the polyclonal antiserum cont作者: 斷言 時間: 2025-3-24 16:31 作者: analogous 時間: 2025-3-24 19:14
DNA Damage Quantitation by Alkaline Gel Electrophoresis,gents, as well as the ability of cells to repair such damages. Quantitative gel electrophoresis of experimental DNAs along with DNA length standards, imaging the resulting dispersed DNA and calculating the population average length allows accurate measurement of lesion frequencies. Number average le作者: transplantation 時間: 2025-3-25 02:01
DNA Repair Protocols978-1-59259-973-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 尋找 時間: 2025-3-25 04:44 作者: Morsel 時間: 2025-3-25 10:01 作者: Veneer 時間: 2025-3-25 13:09 作者: wreathe 時間: 2025-3-25 18:15 作者: 易于交談 時間: 2025-3-25 23:15
Michael Hristov,Christian Weberor ultraviolet light to challenge cells to repair the induced damage, and chromosome aberrations as a biomarker to indicate DNA repair proficiency. The assay was used successfully to demonstrate base- and nucleotide-excision repair deficiency in certain polymorphic DNA repair genes, namely . 751Gln 作者: gnarled 時間: 2025-3-26 02:46
Clinical Classification of Arterial Graftsced genomic instability. Perhaps the best characterized is the dynamic production of chromosomal rearrangements in some clonally expanded cells surviving irradiation. In this chapter we provide the protocols for irradiation, cell culture, chromosome analysis, and characterization of the status of ge作者: d-limonene 時間: 2025-3-26 08:18 作者: 陰郁 時間: 2025-3-26 09:14
M. Durairaj MS, MCh,B. Buxton MBBS, FRACStigations over the last 40 yr. Recent studies have identified several components of this checkpoint response and there is strong interest in its biochemical characterization. Helpful for the delineation of the mechanism of the S-phase checkpoint is the observation that factors inhibiting DNA replica作者: largesse 時間: 2025-3-26 12:44 作者: PLIC 時間: 2025-3-26 18:27
E. Berreklouw MD, PhD,G. -W. He MD, PhD, DScde of cell death. This chapter describes methods to label . DNA strand breaks with fluorochromes for detection by flow or laser scanning cytometry. By staining DNA with a fluorochrome of another color, cellular DNA content is measured concurrently and the bivariate analysis of such a data reveals DN作者: 現(xiàn)任者 時間: 2025-3-26 22:19 作者: Obstreperous 時間: 2025-3-27 04:46 作者: 我不怕犧牲 時間: 2025-3-27 08:14
Aortic Response to Various Effects,ells of higher eukaryotes to repair this lesion, nonhomologous end-joining (NHEJ) is the most dominant. The biochemical characterization of NHEJ has significantly benefited from in vitro plasmid end-joining assays that can complement and extend information obtained from genetic studies. There is evi作者: 友好 時間: 2025-3-27 10:09
https://doi.org/10.1007/978-1-4684-3243-5seases. Gene targeting approaches are also useful for studying the mechanisms of homologous recombination. We have developed gene targeting methods that we have specifically used to investigate the mechanisms of recombination in cultured mammalian cells. In this chapter, we describe the generation o作者: 無能力 時間: 2025-3-27 15:37
William D. Wagner,Thomas B. Clarksonth high reproducibility. Specific nicking and loss of a restricted DNA fragment at the site of induced damage is visualized by Southern blot and quantified against a control; since the blot is gene specific, only the damage of interest is measured. Here we show how the assay may be adapted to assess作者: ABHOR 時間: 2025-3-27 18:06 作者: 饑荒 時間: 2025-3-28 01:08
Jack C. Geer,William S. Webster the genome is modulated by the DNA sequence, by DNA methylation patterns, by the transcriptional status of the locus, and by chromatin proteins associated with the DNA. The only method currently available to allow a precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated作者: 有其法作用 時間: 2025-3-28 03:10
Wolfgang Schaper,Dimitri Scholzeous genetic damage has developed. This, in turn, has increased interest in the cellular mechanisms responsible for tumorigenesis, and the need to develop experimental methodologies to investigate these mechanisms. DNA represents a most important cellular target for ultraviolet radiation (UVR), lead作者: Ibd810 時間: 2025-3-28 09:36 作者: arthroscopy 時間: 2025-3-28 12:03
Eric Allaire,Peter Libby,Alexander W. Clowesver other analytical procedures currently used to measure DNA damage including adaptability, sensitivity and selectivity. This combination of attributes allows for the development of powerful analytical techniques to visualize and quantify specific types of DNA damage in cells and organisms exposed 作者: 新鮮 時間: 2025-3-28 15:41 作者: Anal-Canal 時間: 2025-3-28 21:07 作者: 首創(chuàng)精神 時間: 2025-3-29 01:26 作者: Introduction 時間: 2025-3-29 05:45 作者: 難理解 時間: 2025-3-29 08:25 作者: Morbid 時間: 2025-3-29 11:38
1064-3745 ght together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new s作者: GNAW 時間: 2025-3-29 17:57 作者: 口音在加重 時間: 2025-3-29 21:36 作者: FATAL 時間: 2025-3-30 03:09
Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating,enized cell populations are screened for cells with an increased sensitivity to various mutagens using a replica-plating method. Mutagen-sensitive clones are identified and then characterized by assessing their stability, degree of sensitivity to various mutagens, and by genetic complementation analysis.作者: 使害怕 時間: 2025-3-30 05:23
Endothelial Function in Health and Diseases to UV irradiation of XP keratinocytes, and to objectively determine the extent to which cutaneous gene therapy may be realized, we set up experimental procedures adapted to ex vivo genetic complementation of keratinocytes from XP patients. We provide here detailed rationales and procedures for these approaches.作者: 松軟無力 時間: 2025-3-30 10:27 作者: 飛鏢 時間: 2025-3-30 15:00 作者: 時代錯誤 時間: 2025-3-30 20:13 作者: linear 時間: 2025-3-31 00:00 作者: parsimony 時間: 2025-3-31 04:23 作者: EXULT 時間: 2025-3-31 05:46
Eric Allaire,Peter Libby,Alexander W. Clowesltures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.作者: 晚間 時間: 2025-3-31 12:51
Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes,s to UV irradiation of XP keratinocytes, and to objectively determine the extent to which cutaneous gene therapy may be realized, we set up experimental procedures adapted to ex vivo genetic complementation of keratinocytes from XP patients. We provide here detailed rationales and procedures for these approaches.作者: 放肆的我 時間: 2025-3-31 16:13
Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities,ociated with a significantly increased incidence of both cancer morbidity and mortality, the challenge assay may be useful in predicting cancer risk. The protocol for the assay is straightforward and the data have practical applications.作者: 不近人情 時間: 2025-3-31 18:59
Inhibition of DNA Synthesis by Ionizing Radiation,d MRE11, as well as downstream modulators can provoke an RDS phenotype. Here a simple, accurate and highly reproducible experimental protocol is presented for the generation of DNA synthesis inhibition curves from cells in culture.作者: jettison 時間: 2025-3-31 22:07 作者: 使激動 時間: 2025-4-1 05:51
Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian re we discuss advantages and limitations of using QPCR to assay DNA damage in mammalian cells. In addition, we give a detailed protocol for the QPCR assay that helps facilitate its successful deployment in any molecular biology laboratory.作者: biosphere 時間: 2025-4-1 06:06
Immunochemical Detection of UV-Induced DNA Damage and Repair, which recognize cyclobutane thymine dimers (T-T). Immuno-approaches have a number of benefits over chromatographic techniques, and have been applied herein to quantitatively and qualitatively assess the presence of T-T in cultured keratinocytes, human skin, and urine, providing information about lesion induction and repair.
