標題: Titlebook: DNA Repair; Methods and Protocol Lata Balakrishnan,Jason A. Stewart Book 2019 Springer Science+Business Media, LLC, part of Springer Nature [打印本頁] 作者: 從未迷惑 時間: 2025-3-21 19:55
書目名稱DNA Repair影響因子(影響力)
書目名稱DNA Repair影響因子(影響力)學科排名
書目名稱DNA Repair網(wǎng)絡公開度
書目名稱DNA Repair網(wǎng)絡公開度學科排名
書目名稱DNA Repair被引頻次
書目名稱DNA Repair被引頻次學科排名
書目名稱DNA Repair年度引用
書目名稱DNA Repair年度引用學科排名
書目名稱DNA Repair讀者反饋
書目名稱DNA Repair讀者反饋學科排名
作者: infelicitous 時間: 2025-3-21 21:51
Resolving Roadblocks to Telomere Replicationon. The terminal position, repetitive sequence, and structural complexities of the telomeric DNA make the telomere an inherently difficult region to replicate within the genome. Thus, despite functioning to protect genome stability mammalian telomeres are also a source of replication stress and have作者: 江湖郎中 時間: 2025-3-22 01:48 作者: 狂怒 時間: 2025-3-22 07:53 作者: micturition 時間: 2025-3-22 09:35
Methods to Study Trinucleotide Repeat Instability Induced by DNA Damage and Repaired by DNA replication, repair, recombination, and gene transcription. Somatic TNR instability is involved in the progression of TNR expansion diseases and can be modulated by DNA damage repair and gene transcription. Recent studies from our group and others have shown that DNA base damage and its re作者: Mangle 時間: 2025-3-22 14:30
Extracting and Measuring dNTP Pools in ility. Ribonucleotide reductase (RNR) catalyzes the rate-limiting step in dNTP synthesis and altered regulation of RNR leads to imbalanced dNTP pools. Increased dNTP levels are mutagenic and have the potential to interfere with pathways that are involved in DNA replication, repair and DNA damage con作者: Mangle 時間: 2025-3-22 17:03
Conversion Tract Analysis of Homology-Directed Genome Editing Using Oligonucleotide Donorsnversion tract is defined as the amount of genetic information that is converted from the homology donor to a given strand of the targeted chromosomal locus. Because of this, conversion tract analysis retrospectively not only elucidates the mechanism of homology-directed genome editing but also prov作者: 真繁榮 時間: 2025-3-22 21:36
Reporter Assays for BER Pathwayamages induced by free radicals and ionizing radiation. Deregulation or deficiencies in BER mechanisms increase the level of mutations leading to carcinogenesis, and single-strand DNA break formation, which may be converted to double-strand breaks and induce apoptosis. BER deficiency is associated w作者: 服從 時間: 2025-3-23 03:40
Methods for Studying DNA Single-Strand Break Repair and Signaling in , Egg ExtractsUnrepaired SSBs compromise DNA replication and transcription programs, leading to genome instability, and have been implicated in many diseases including cancer. In this chapter, we introduce methods to study the ATR-Chk1 DNA damage response (DDR) pathway and DNA repair pathway in response to a site作者: 一致性 時間: 2025-3-23 05:58
Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in , Egg Extractsitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and . egg extracts provides a highly versatile and tractable approach to analyze dynamic protein–DNA interactions with great spatial and temporal detail.作者: GONG 時間: 2025-3-23 11:23
Cellular Assays to Study the Functional Importance of Human DNA Repair Helicases binding and hydrolysis, helicases behave as molecular motors capable of efficiently disrupting the many noncovalent hydrogen bonds that stabilize DNA molecules with secondary structure. In addition to their importance in DNA damage sensing and signaling, DNA helicases facilitate specific steps in D作者: 驕傲 時間: 2025-3-23 14:40 作者: Conflict 時間: 2025-3-23 18:54 作者: acolyte 時間: 2025-3-23 23:45 作者: Blanch 時間: 2025-3-24 04:29 作者: 退潮 時間: 2025-3-24 07:50
DNA Repair978-1-4939-9500-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Annotate 時間: 2025-3-24 12:23 作者: Celiac-Plexus 時間: 2025-3-24 16:50 作者: 消毒 時間: 2025-3-24 20:52
978-1-4939-9502-8Springer Science+Business Media, LLC, part of Springer Nature 2019作者: 種植,培養(yǎng) 時間: 2025-3-25 01:10 作者: liposuction 時間: 2025-3-25 03:49 作者: 參考書目 時間: 2025-3-25 10:46
Attention Deficit Hyperactivity Disorder,on. The terminal position, repetitive sequence, and structural complexities of the telomeric DNA make the telomere an inherently difficult region to replicate within the genome. Thus, despite functioning to protect genome stability mammalian telomeres are also a source of replication stress and have作者: temperate 時間: 2025-3-25 13:01
Scintillation and Apparent Motion,es. These highly dynamic processes have been widely studied using laser microirradiation coupled with fluorescence microscopy. Laser microirradiation has provided a powerful methodology to identify and determine mechanisms of DNA damage response pathways. Here we describe methods used to analyze pro作者: periodontitis 時間: 2025-3-25 18:38 作者: propose 時間: 2025-3-25 22:37
Susana Gon?alves,Suzanne Majhanoviched by DNA replication, repair, recombination, and gene transcription. Somatic TNR instability is involved in the progression of TNR expansion diseases and can be modulated by DNA damage repair and gene transcription. Recent studies from our group and others have shown that DNA base damage and its re作者: 駕駛 時間: 2025-3-26 03:39 作者: corpus-callosum 時間: 2025-3-26 04:57 作者: CHOIR 時間: 2025-3-26 09:05
Kelly Comfort (Assistant Professor)amages induced by free radicals and ionizing radiation. Deregulation or deficiencies in BER mechanisms increase the level of mutations leading to carcinogenesis, and single-strand DNA break formation, which may be converted to double-strand breaks and induce apoptosis. BER deficiency is associated w作者: angina-pectoris 時間: 2025-3-26 15:58 作者: 龍蝦 時間: 2025-3-26 19:28
Kelly Comfort (Assistant Professor)itation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and . egg extracts provides a highly versatile and tractable approach to analyze dynamic protein–DNA interactions with great spatial and temporal detail.作者: 替代品 時間: 2025-3-26 23:13
https://doi.org/10.1007/978-1-349-00952-7 binding and hydrolysis, helicases behave as molecular motors capable of efficiently disrupting the many noncovalent hydrogen bonds that stabilize DNA molecules with secondary structure. In addition to their importance in DNA damage sensing and signaling, DNA helicases facilitate specific steps in D作者: Stagger 時間: 2025-3-27 01:06
Creativity and Parkinson’s Disease was established by measuring the resistance capacity of .deficient mouse embryonic fibroblasts (MEFs) transfected with mammalian expression constructs. Here, we describe the methods used to assess colony survival following the treatment of transfected cells with genotoxic compounds. This approach p作者: Indolent 時間: 2025-3-27 08:29
https://doi.org/10.1007/978-3-031-14724-1getable proteins beyond kinases and traditional enzymes. Many of the proteins involved in the DDR and repair impart their activity by making specific contacts with DNA. These protein–nucleic acid interactions represent a tractable target for perturbation with small molecules. We describe a high thro作者: 單獨 時間: 2025-3-27 09:52 作者: finite 時間: 2025-3-27 16:44 作者: Affirm 時間: 2025-3-27 21:24
Lata Balakrishnan,Jason A. StewartIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 縱火 時間: 2025-3-27 22:57
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260176.jpg作者: 冷漠 時間: 2025-3-28 02:50
Attention Deficit Hyperactivity Disorder,hat ALT telomeres are subjected to chronic replication stress. Here, we will review the features of telomeric DNA that challenge the replication machinery and also how the cell overcomes these challenges to maintain telomere stability and ensure the faithful duplication of the human genome.作者: 不如屎殼郎 時間: 2025-3-28 10:02
Scintillation and Apparent Motion, Together, we aim to provide robust visualization methods employing laser-microirradiation to detect and determine protein behavior, functions and dynamics in response to DNA damage in mammalian cells.作者: 船員 時間: 2025-3-28 12:08
Shared Reflections and Dialoguesve information about dNTP pools in different genetic and environmental contexts in vivo. Here we describe a high-throughput fluorescence-based assay that uses a qPCR-based approach to quantify dNTP levels for use with . extracts.作者: 舊石器 時間: 2025-3-28 15:01 作者: 滲入 時間: 2025-3-28 19:25 作者: HAIRY 時間: 2025-3-29 00:41
In Time and Space: Laser Microirradiation and the DNA Damage Response Together, we aim to provide robust visualization methods employing laser-microirradiation to detect and determine protein behavior, functions and dynamics in response to DNA damage in mammalian cells.作者: vascular 時間: 2025-3-29 04:24
Extracting and Measuring dNTP Pools in ve information about dNTP pools in different genetic and environmental contexts in vivo. Here we describe a high-throughput fluorescence-based assay that uses a qPCR-based approach to quantify dNTP levels for use with . extracts.作者: 圖表證明 時間: 2025-3-29 10:38 作者: Ligneous 時間: 2025-3-29 12:58
Methods for Studying DNA Single-Strand Break Repair and Signaling in , Egg Extracts-specific, defined SSB plasmid in . egg extracts. This experimental system can be applied in future studies to reveal many aspects of the molecular mechanisms of SSB repair and signaling in eukaryotes.作者: Cpr951 時間: 2025-3-29 15:47 作者: BIBLE 時間: 2025-3-29 22:07 作者: 極微小 時間: 2025-3-30 01:55
Vision, Illusion and PerceptionGE has the capacity to separate DNA molecules from several hundred base pairs to over six million base pairs. In the method described here, molecules from five hundred thousand to three million base pairs are consolidated into a single band on the gel that is readily analyzed.作者: 突變 時間: 2025-3-30 06:54
Creativity and Parkinson’s Diseases. Here, we describe the methods used to assess colony survival following the treatment of transfected cells with genotoxic compounds. This approach provides a time-efficient and stringent strategy to screen genetic alleles for identifying regions or specific amino acid residues critical for function or regulation of DNA repair pathways.作者: PALSY 時間: 2025-3-30 09:19 作者: 膽小懦夫 時間: 2025-3-30 13:59 作者: 審問,審訊 時間: 2025-3-30 19:35
Kelly Comfort (Assistant Professor)-specific, defined SSB plasmid in . egg extracts. This experimental system can be applied in future studies to reveal many aspects of the molecular mechanisms of SSB repair and signaling in eukaryotes.作者: Carminative 時間: 2025-3-30 23:23
https://doi.org/10.1007/978-3-031-14724-1ughput, solution-based equilibrium binding fluorescence polarization assay that can be applied to a wide array of protein–nucleic acid interactions. The assay is sensitive, stable, and able to identify small molecules capable of blocking DNA–protein interactions.作者: colloquial 時間: 2025-3-31 00:51
Kelly Comfort (Assistant Professor)uorescent protein conversion system that can be conveniently used to measure the efficiency and analyze the lengths of conversion tracts of homology-directed genome editing using oligonucleotide donors in mammalian cells.作者: 串通 時間: 2025-3-31 05:51
https://doi.org/10.1057/978-1-349-95016-4ctivity using radiolabeled ATP. Additionally, we describe a high-throughput coupled reaction assay pairing ATP hydrolysis with nicotinamide adenine dinucleotide (NADH) oxidation to monitor ATP hydrolysis in real time.作者: 社團 時間: 2025-3-31 10:14 作者: vanquish 時間: 2025-3-31 14:51
Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysisctivity using radiolabeled ATP. Additionally, we describe a high-throughput coupled reaction assay pairing ATP hydrolysis with nicotinamide adenine dinucleotide (NADH) oxidation to monitor ATP hydrolysis in real time.作者: 確定的事 時間: 2025-3-31 17:56
Book 2019ve sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by defective telomere replication. Part II describes methods to detect and measure DNA breaks and proteins involved in DNA damage response. Part作者: 吝嗇性 時間: 2025-4-1 01:11
Homologous Recombination-Mediated DNA Repair and Implications for Clinical Treatment of Repair Defecng during DSB repair. In addition, clinical phenotypes associated with HR-defective cancers, such as mutational signatures used to predict response to poly(ADP-ribose) polymerase inhibitors, are discussed. Understanding these processes will provide insight into mechanisms of genome maintenance and l作者: 虛度 時間: 2025-4-1 02:48
Methods to Study Trinucleotide Repeat Instability Induced by DNA Damage and Repairic TNR instability is critically important for identification of new therapeutic targets for treatment and prevention of TNR-related diseases. Here we describe the methods to study the locations and distribution of DNA base lesions and their effects on TNR instability through DNA base excision repai
