標(biāo)題: Titlebook: DNA Recombination; Methods and Protocol Hideo Tsubouchi Book 2011 Springer Science+Business Media, LLC 2011 DNA double-strand break.DNA mic [打印本頁] 作者: 萬能 時(shí)間: 2025-3-21 18:28
書目名稱DNA Recombination影響因子(影響力)
書目名稱DNA Recombination影響因子(影響力)學(xué)科排名
書目名稱DNA Recombination網(wǎng)絡(luò)公開度
書目名稱DNA Recombination網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱DNA Recombination被引頻次
書目名稱DNA Recombination被引頻次學(xué)科排名
書目名稱DNA Recombination年度引用
書目名稱DNA Recombination年度引用學(xué)科排名
書目名稱DNA Recombination讀者反饋
書目名稱DNA Recombination讀者反饋學(xué)科排名
作者: 無法破譯 時(shí)間: 2025-3-21 23:04
Molecular Assays to Investigate Chromatin Changes During DNA Double-Strand Break Repair in Yeastresents to the repair process. In this article, we describe molecular techniques available to analyze chromatin architecture near a defined DSB in budding yeast. These techniques may be of value to experimentalists who are investigating the role of a novel protein in DSB repair specifically in the c作者: anchor 時(shí)間: 2025-3-22 01:53
Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40hanisms in HRR. Using these techniques, a role of HRR of not only Rad52 epistasis group genes but also genes whose mutation cause hereditary cancer syndrome, such as Fanconi anemia, has been established.作者: BRAWL 時(shí)間: 2025-3-22 07:30 作者: 金桌活畫面 時(shí)間: 2025-3-22 10:49 作者: 大量 時(shí)間: 2025-3-22 15:27
Geetika Saini,Sunil Kumar,Ranjit Kumard DNA, resection of random DSBs can be easily detected and analyzed. This mobility-shift assay provides a unique opportunity to examine the mechanisms of resection, early events in DSB repair, as well as factors involved in pathway regulation.作者: 大量 時(shí)間: 2025-3-22 18:03
https://doi.org/10.1007/978-3-030-61006-7resents to the repair process. In this article, we describe molecular techniques available to analyze chromatin architecture near a defined DSB in budding yeast. These techniques may be of value to experimentalists who are investigating the role of a novel protein in DSB repair specifically in the c作者: 鳥籠 時(shí)間: 2025-3-23 01:13 作者: 障礙 時(shí)間: 2025-3-23 05:26 作者: Factual 時(shí)間: 2025-3-23 07:28 作者: 其他 時(shí)間: 2025-3-23 10:57 作者: 細(xì)頸瓶 時(shí)間: 2025-3-23 13:59
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260174.jpg作者: synovial-joint 時(shí)間: 2025-3-23 18:25
DNA Recombination978-1-61779-129-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: obligation 時(shí)間: 2025-3-24 00:29
The Journey of Arsenic from Soil to Planteave recombinogenic substrates such as single-stranded DNA gaps. Other types of damages result in general genome instability such as chromosome loss, chromosome fragmentation, and chromosome rearrangements. The genome is kept intact through recombination, repair, replication, checkpoints, and chromo作者: 哀悼 時(shí)間: 2025-3-24 03:11 作者: 施加 時(shí)間: 2025-3-24 08:11
https://doi.org/10.1007/978-3-030-61006-7nd breaks (DSBs) by a conserved meiosis-specific protein, Spo11. Meiotic DSBs are not formed at random along chromosomes but are formed in clusters known as recombination hot spots. To understand the regulation of this initiation step of meiotic recombination, determining the timing and location of 作者: Rodent 時(shí)間: 2025-3-24 11:36
https://doi.org/10.1007/978-3-030-61006-7ome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites during recombination. The protocol outlined here has b作者: 蟄伏 時(shí)間: 2025-3-24 14:54
https://doi.org/10.1007/978-3-030-61006-7 DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase–DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked 作者: FADE 時(shí)間: 2025-3-24 19:01
https://doi.org/10.1007/978-3-030-61006-7genomes of eukaryotes. DSBs form normally during a variety of biological processes, such as V–D–J recombination and yeast mating type switching, but unprogrammed DSBs are among the most dangerous types of lesion because if left unrepaired they can lead to loss of genetic material or chromosomal rear作者: 艦旗 時(shí)間: 2025-3-25 02:48
The Police Investigation of Art Fraudrmed via several branched intermediates, including single end invasions and double Holliday junctions. Two-dimensional gel electrophoresis provides a sensitive and specific approach for detecting branched recombination intermediates, determining their genetic requirements, and enriching intermediate作者: Bronchial-Tubes 時(shí)間: 2025-3-25 03:46
Saskia Hufnagel,Duncan Chappellysical bridges between homologs that provide the appropriate tension to properly align chromosomes on the meiosis I spindle. Homolog pairs that fail to cross over can result in meiosis I nondisjunction, leading to aneuploid gametes. Therefore, the number and distribution of crossovers are tightly re作者: Parameter 時(shí)間: 2025-3-25 09:04
https://doi.org/10.1007/978-1-137-40757-3iosis in budding yeast by suppressing recombination between sister chromatids, as well as playing a role in the meiotic recombination checkpoint. Understanding how Mek1 regulates recombination requires the identification of direct substrates of the kinase. We have applied the semi-synthetic epitope 作者: 光亮 時(shí)間: 2025-3-25 13:04 作者: Pantry 時(shí)間: 2025-3-25 17:47 作者: 枯燥 時(shí)間: 2025-3-25 20:27 作者: PHIL 時(shí)間: 2025-3-26 04:13 作者: 謙卑 時(shí)間: 2025-3-26 04:59
https://doi.org/10.1007/978-94-009-1594-7ich preferentially occur at hotspots dispersed throughout the genome. These double-strand breaks are repaired from the homolog, resulting in either a crossover or noncrossover product. Multiple noncrossover events are required for homolog pairing, and at least one crossover is critical for proper ch作者: 激勵(lì) 時(shí)間: 2025-3-26 08:49 作者: 減震 時(shí)間: 2025-3-26 13:18
https://doi.org/10.1007/978-1-4020-9989-2omologous DNA fragment by HR is usually a very rare event in vertebrate cells; however, in chicken B lymphoma cell line DT40, the ratio of targeted to random integration is extremely high. Although the underlying mechanism of this phenotype is not fully understood, DT40 has been utilized as a model 作者: ARBOR 時(shí)間: 2025-3-26 18:51 作者: 膝蓋 時(shí)間: 2025-3-26 21:57 作者: CANON 時(shí)間: 2025-3-27 03:01
Methods to Study Mitotic Homologous Recombination and Genome Stabilityeave recombinogenic substrates such as single-stranded DNA gaps. Other types of damages result in general genome instability such as chromosome loss, chromosome fragmentation, and chromosome rearrangements. The genome is kept intact through recombination, repair, replication, checkpoints, and chromo作者: notification 時(shí)間: 2025-3-27 06:42
Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Endsrminant in choice of repair pathways and eventual genome stability. Current techniques for examining resection are restricted to model in vivo systems with defined substrates (i.e., HO-endonuclease targets). We present here a robust assay that can analyze not only the resection of site-specific DSBs作者: 騎師 時(shí)間: 2025-3-27 11:07 作者: rheumatology 時(shí)間: 2025-3-27 14:18
Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-Stranded DNAome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites during recombination. The protocol outlined here has b作者: watertight, 時(shí)間: 2025-3-27 21:02
Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase–DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked 作者: 先驅(qū) 時(shí)間: 2025-3-28 00:35 作者: 松雞 時(shí)間: 2025-3-28 04:40
Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresisrmed via several branched intermediates, including single end invasions and double Holliday junctions. Two-dimensional gel electrophoresis provides a sensitive and specific approach for detecting branched recombination intermediates, determining their genetic requirements, and enriching intermediate作者: 得意人 時(shí)間: 2025-3-28 09:55
Mapping of Crossover Sites Using DNA Microarraysysical bridges between homologs that provide the appropriate tension to properly align chromosomes on the meiosis I spindle. Homolog pairs that fail to cross over can result in meiosis I nondisjunction, leading to aneuploid gametes. Therefore, the number and distribution of crossovers are tightly re作者: 易于出錯(cuò) 時(shí)間: 2025-3-28 13:18 作者: landfill 時(shí)間: 2025-3-28 16:28 作者: tic-douloureux 時(shí)間: 2025-3-28 21:06 作者: Limpid 時(shí)間: 2025-3-28 23:09
Detection of RNA-Templated Double-Strand Break Repair in Yeastg through a reverse transcript intermediate. As initially demonstrated in the yeast . via transformation by RNA-containing oligonucleotides (oligos), RNA sequences can serve as templates for chromosomal double-strand break (DSB) repair. Synthetic oligos containing embedded RNA tracts of various size作者: 機(jī)密 時(shí)間: 2025-3-29 06:51
Characterization of Meiotic Crossovers in Pollen from ,anistically important for the regular segregation of homologous chromosomes. They generate either crossovers, which are reciprocal exchanges between chromosome segments, or gene conversions. Both kinds of events occur in narrow regions (less than 10?kb) called hotspots, which are distributed along c作者: 有角 時(shí)間: 2025-3-29 10:31 作者: BUST 時(shí)間: 2025-3-29 11:38 作者: 前面 時(shí)間: 2025-3-29 18:35
Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40omologous DNA fragment by HR is usually a very rare event in vertebrate cells; however, in chicken B lymphoma cell line DT40, the ratio of targeted to random integration is extremely high. Although the underlying mechanism of this phenotype is not fully understood, DT40 has been utilized as a model 作者: leniency 時(shí)間: 2025-3-29 22:20 作者: 破裂 時(shí)間: 2025-3-30 00:28 作者: 撫慰 時(shí)間: 2025-3-30 05:32
https://doi.org/10.1007/978-1-137-40757-3log, for kinase assays that detect only those proteins that are directly phosphorylated by Mek1. This method may be applicable to any kinase for which an analog-sensitive version is available. In addition, it provides a non-radioactive alternative for kinase assays with wild-type kinases.作者: 比喻好 時(shí)間: 2025-3-30 10:49
The Relevance of Beautiful Infrastructurewhich cells can use RNA to repair DNA damage such as a DSB are yet to be identified. Here we show the approach we utilize to detect repair of a chromosomal DSB by RNA-containing oligos in yeast cells.作者: 帶子 時(shí)間: 2025-3-30 12:58 作者: 愛花花兒憤怒 時(shí)間: 2025-3-30 17:33 作者: 無力更進(jìn) 時(shí)間: 2025-3-30 22:30
Methods to Study Mitotic Homologous Recombination and Genome Stabilityr a subset of the genomic instabilities: spontaneous mitotic recombination and chromosome loss, in both haploid and diploid cells. The assays, while not inclusive of all genome instability assays, give a broad assessment of general genome damage or inability to repair damage in various genetic backgrounds.作者: 生氣的邊緣 時(shí)間: 2025-3-31 02:49
Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresislso provide an introduction to meiotic time-course procedures, stabilization of branched DNA molecules by interstrand crosslinking, extraction of genomic DNA from meiotic cultures, and quantitative analysis of two-dimensional gel blots.作者: entail 時(shí)間: 2025-3-31 08:39
Using the Semi-synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Buddinlog, for kinase assays that detect only those proteins that are directly phosphorylated by Mek1. This method may be applicable to any kinase for which an analog-sensitive version is available. In addition, it provides a non-radioactive alternative for kinase assays with wild-type kinases.作者: 會(huì)議 時(shí)間: 2025-3-31 11:51
Detection of RNA-Templated Double-Strand Break Repair in Yeastwhich cells can use RNA to repair DNA damage such as a DSB are yet to be identified. Here we show the approach we utilize to detect repair of a chromosomal DSB by RNA-containing oligos in yeast cells.作者: 粘連 時(shí)間: 2025-3-31 15:06
