作者: anniversary 時(shí)間: 2025-3-21 23:32
Ultrasensitive Detection of Metal Ions with DNA Nanostructure,, the detection limit is down to 10, 10, and 20?nM for Hg., Ag., and Pb., respectively. Therefore, the DNM biosensor capable of simultaneously detecting multiple heavy metal ions with sensitivity and selectivity shows great potential to be point-of-test devices.作者: 肉身 時(shí)間: 2025-3-22 01:55
LSPR Detection of Nucleic Acids on Nanoparticle Monolayers, this system will be presented. A dense layer of noble metal nanoparticles is immobilized on a glass substrate and implemented in a microfluidic chamber, where the spectroscopic measurements are conducted.作者: 墻壁 時(shí)間: 2025-3-22 05:10
Elizabeth Merz Butterfield (1896–1947), the detection limit is down to 10, 10, and 20?nM for Hg., Ag., and Pb., respectively. Therefore, the DNM biosensor capable of simultaneously detecting multiple heavy metal ions with sensitivity and selectivity shows great potential to be point-of-test devices.作者: crease 時(shí)間: 2025-3-22 09:43
https://doi.org/10.1007/978-1-4614-7461-6 this system will be presented. A dense layer of noble metal nanoparticles is immobilized on a glass substrate and implemented in a microfluidic chamber, where the spectroscopic measurements are conducted.作者: Respond 時(shí)間: 2025-3-22 13:53 作者: Respond 時(shí)間: 2025-3-22 18:38
Armutsberichterstattung in DeutschlandThe founding of structural DNA nanotechnology is described, birth pangs and all by the originator of the field. The excitement of the invention, the characters, and the roles are evident as a true celebration of scientific research.作者: HPA533 時(shí)間: 2025-3-22 21:24 作者: Genome 時(shí)間: 2025-3-23 02:03 作者: Innocence 時(shí)間: 2025-3-23 07:12 作者: 險(xiǎn)代理人 時(shí)間: 2025-3-23 11:17 作者: landmark 時(shí)間: 2025-3-23 16:37 作者: Charade 時(shí)間: 2025-3-23 20:49
DNA Nanotechnology978-1-4939-8582-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 過(guò)時(shí) 時(shí)間: 2025-3-23 23:04 作者: 會(huì)議 時(shí)間: 2025-3-24 06:11 作者: AVOID 時(shí)間: 2025-3-24 08:49 作者: 一起 時(shí)間: 2025-3-24 12:28 作者: Lymphocyte 時(shí)間: 2025-3-24 16:20
Margot Becke-Goehring,Margaret EuckenPNAs into inorganic nanocarriers can improve their cellular uptake and co-delivering them together with drugs can improve the therapy efficacy by synergic effects. Furthermore, the functionalization of the carriers with labels allows theranostics, and the possibility to monitor the efficacy of the t作者: 熱情的我 時(shí)間: 2025-3-24 22:37 作者: Handedness 時(shí)間: 2025-3-25 03:03 作者: 交響樂(lè) 時(shí)間: 2025-3-25 06:09
Elizabeth Merz Butterfield (1896–1947)vy metal contamination remains challenging. Here we develop a DNA nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography for rapid and sensitive multiplex detection of heavy metal ions. In our design, DNA tetrahedral-structured probes (TSPs) ar作者: Saline 時(shí)間: 2025-3-25 07:33
https://doi.org/10.1007/978-1-4614-7461-6ication. Here, we put forward a novel experimental-based approach that combines DNA-directed immobilization, nanografting, and atomic force height measurements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which c作者: 開(kāi)玩笑 時(shí)間: 2025-3-25 12:40 作者: BRACE 時(shí)間: 2025-3-25 17:23 作者: Anthrp 時(shí)間: 2025-3-25 22:12 作者: Intentional 時(shí)間: 2025-3-26 00:33
https://doi.org/10.1007/978-1-4614-7461-6and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved λ-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and alig作者: Morphine 時(shí)間: 2025-3-26 06:21
https://doi.org/10.1007/b138219ethod has emerged as a powerful computational tool for atomic-resolution characterization of the in situ structure and physical properties of DNA origami objects. This chapter provides step-by-step instructions for building atomic-scale models of DNA origami systems, using the MD method to simulate 作者: 完整 時(shí)間: 2025-3-26 09:11
https://doi.org/10.1007/978-3-642-79373-8th single-molecule control. We combine the ability of DNA nanostructures as programmable platforms, with a one-step Focused Ion Beam nanopatterning, to demonstrate the controlled immobilization of DNA origami functionalized with individual quantum dots (QDs) at predesigned positions on glass coversl作者: ANT 時(shí)間: 2025-3-26 15:10
Compounds Derived from Aminoacetic Acidsons of DNA origami nanostructures, their use as masks for patterning of organic and inorganic materials by molecular lithography has received great attention. Here, we describe a protocol for the self-assembly of ordered monolayers of DNA origami nanostructures on mica surfaces and the subsequent fa作者: 幻想 時(shí)間: 2025-3-26 19:54
https://doi.org/10.1007/978-1-4020-9787-4DNA origami and incubate them onto arrays of anchoring gold nanodots either in a static solution or in a microfluidic system. We give details on the design and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and 作者: panorama 時(shí)間: 2025-3-26 23:22 作者: 言外之意 時(shí)間: 2025-3-27 03:37
Giampaolo ZuccheriIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 招惹 時(shí)間: 2025-3-27 06:39
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260171.jpg作者: 等級(jí)的上升 時(shí)間: 2025-3-27 12:05
The Synthesis of Designer DNA, here describes an enzymatic method for the synthesis of long repeat-sequence DNA. The method utilizes a PCR-based approach; starting with short oligo-seeds, c.a. 20?bp, bearing a minimum of two repeating units of >8?bp sequences. During each heat-cool cycle, the oligo-seeds reanneal imperfectly lea作者: 放縱 時(shí)間: 2025-3-27 16:48 作者: 不來(lái) 時(shí)間: 2025-3-27 19:34
Preparation of Anti-miR PNAs for Drug Development and Nanomedicine,st-transcriptionally. PNAs are obtained with solid-phase peptide synthesis, and can be easily conjugated to other peptides. Conjugation with R8-Peptide or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes 作者: indubitable 時(shí)間: 2025-3-27 22:00 作者: Scleroderma 時(shí)間: 2025-3-28 03:11 作者: PALSY 時(shí)間: 2025-3-28 09:07
Hybridization Chain Reaction Design and Biosensor Implementation,in impediments for such applications of DNA biosensors is their lack of sensitivity: this can prevent their full exploitation in the diagnostic analytical field. DNA nanotechnology could enhance DNA biosensors and let them perform at the required high sensitivity. Well-designed, programmable self-as作者: 你敢命令 時(shí)間: 2025-3-28 11:51
Ultrasensitive Detection of Metal Ions with DNA Nanostructure,vy metal contamination remains challenging. Here we develop a DNA nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography for rapid and sensitive multiplex detection of heavy metal ions. In our design, DNA tetrahedral-structured probes (TSPs) ar作者: FISC 時(shí)間: 2025-3-28 16:15 作者: 遠(yuǎn)足 時(shí)間: 2025-3-28 20:25 作者: 外觀 時(shí)間: 2025-3-29 00:43 作者: 真實(shí)的人 時(shí)間: 2025-3-29 05:09
Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation oflar detection or separation. We here provide a protocol to perform capture-SELEX for DNA aptamers binding soluble small molecules, which includes a straightforward functional validation by SPR. The SELEX strategy here described is adaptable to any water-soluble molecular target and might foster the 作者: –LOUS 時(shí)間: 2025-3-29 10:57 作者: QUAIL 時(shí)間: 2025-3-29 13:13
A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems,ethod has emerged as a powerful computational tool for atomic-resolution characterization of the in situ structure and physical properties of DNA origami objects. This chapter provides step-by-step instructions for building atomic-scale models of DNA origami systems, using the MD method to simulate 作者: enfeeble 時(shí)間: 2025-3-29 16:34
Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform,th single-molecule control. We combine the ability of DNA nanostructures as programmable platforms, with a one-step Focused Ion Beam nanopatterning, to demonstrate the controlled immobilization of DNA origami functionalized with individual quantum dots (QDs) at predesigned positions on glass coversl作者: licence 時(shí)間: 2025-3-29 22:02 作者: foreign 時(shí)間: 2025-3-29 23:55
DNA Origami Structures Interfaced to Inorganic Nanodevices,DNA origami and incubate them onto arrays of anchoring gold nanodots either in a static solution or in a microfluidic system. We give details on the design and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and 作者: 向宇宙 時(shí)間: 2025-3-30 08:04
Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology,at the nanoscale. Here we show how it is possible to control the plasmonic resonance of complex architectures of gold nanoparticles using the peculiar properties of DNA Watson and Crick pairing rules. In this chapter, we will first introduce all the steps required to generate, purify, and characteri作者: 親屬 時(shí)間: 2025-3-30 10:54
1064-3745 ation advice from the expertsThis second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nu作者: medium 時(shí)間: 2025-3-30 15:13 作者: 休戰(zhàn) 時(shí)間: 2025-3-30 20:35
Margot Becke-Goehring,Margaret Euckene or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes the synthesis of cationic PNAs that can be used alone as drugs or for efficient co-delivery in suitable inorganic nanocarriers.作者: FISC 時(shí)間: 2025-3-31 00:18 作者: FANG 時(shí)間: 2025-3-31 01:15
https://doi.org/10.1007/978-1-4614-7461-6surements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which can bind to the solvent-exposed site on the Beta-2-microglobulin (β2m).作者: Confound 時(shí)間: 2025-3-31 06:31 作者: GUISE 時(shí)間: 2025-3-31 10:34 作者: Insul島 時(shí)間: 2025-3-31 15:27
https://doi.org/10.1007/978-3-642-79373-8o demonstrate the controlled immobilization of DNA origami functionalized with individual quantum dots (QDs) at predesigned positions on glass coverslips and silicon substrates. Remarkably, the platform developed is reusable after a simple cleaning process, and can be designed to display different geometrical arrangements.作者: 運(yùn)氣 時(shí)間: 2025-3-31 18:19
https://doi.org/10.1007/978-1-4020-9787-4esign and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and atomic force microscopy methodological details are also given for obtaining the relevant characterizations of the immobilized and ordered DNA origami.作者: MURAL 時(shí)間: 2025-4-1 01:09
Loading of PNA and Other Molecular Payloads on Inorganic Nanostructures for Theranostics,herapy in real time. The present protocol describes the synthesis of Zeolites-L nanocrystals and mesoporous silica nanoparticles and their loading with cationic PNAs and other smaller molecular weight payloads towards theranostics applications.作者: arbovirus 時(shí)間: 2025-4-1 04:38
Dielectrophoretic Stretching of DNA,n them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.作者: 暫時(shí)中止 時(shí)間: 2025-4-1 09:16
Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology,ze DNA nanostructures, then we will guide the reader to the main steps required to decorate them with a precise amount of gold nanoparticles and, finally, we will describe the main approach used to characterize their plasmonic response.作者: predict 時(shí)間: 2025-4-1 11:28
Compounds Derived from Aminoacetic Acids is determined by the shape of the DNA origami nanostructures, protein coverage inside the holes of the mask can be varied from single proteins to dense monolayers by adjusting the protein concentration and cationic strength of the adsorption buffer.作者: 啞劇 時(shí)間: 2025-4-1 17:17
Directed Protein Adsorption Through DNA Origami Masks, is determined by the shape of the DNA origami nanostructures, protein coverage inside the holes of the mask can be varied from single proteins to dense monolayers by adjusting the protein concentration and cationic strength of the adsorption buffer.
