標(biāo)題: Titlebook: DNA Modifications; Methods and Protocol Alexey Ruzov,Martin Gering Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusi [打印本頁] 作者: Flexibility 時(shí)間: 2025-3-21 16:12
書目名稱DNA Modifications影響因子(影響力)
書目名稱DNA Modifications影響因子(影響力)學(xué)科排名
書目名稱DNA Modifications網(wǎng)絡(luò)公開度
書目名稱DNA Modifications網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱DNA Modifications被引頻次
書目名稱DNA Modifications被引頻次學(xué)科排名
書目名稱DNA Modifications年度引用
書目名稱DNA Modifications年度引用學(xué)科排名
書目名稱DNA Modifications讀者反饋
書目名稱DNA Modifications讀者反饋學(xué)科排名
作者: hemoglobin 時(shí)間: 2025-3-21 20:32 作者: 不要嚴(yán)酷 時(shí)間: 2025-3-22 03:01
MS Analysis of DNA Modifications in Urinary/Body Fluidswo-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS) and high-performance liquid chromatography coupled with gas chromatography and mass spectrometry (HPLC/GC/MS).作者: evaculate 時(shí)間: 2025-3-22 07:43 作者: flutter 時(shí)間: 2025-3-22 10:20
Lecture Notes in Computer Science reach. Here, we review the techniques currently available for profiling DNA modifications in mammals, discuss their strengths and weaknesses, and speculate on the future direction of DNA modification profiling technologies.作者: slipped-disk 時(shí)間: 2025-3-22 16:22
https://doi.org/10.1007/978-3-319-16277-5ced a similar rate of development and is often a bottleneck to chemical analysis. Here we present a protocol for identification and quantification of cytosine modifications that combines a robust and efficient method to generate single nucleosides from genomic DNA samples followed by direct LC-MS/MS analysis.作者: slipped-disk 時(shí)間: 2025-3-22 19:11
Mapping DNA Methylation in Mammals: The State of the Art reach. Here, we review the techniques currently available for profiling DNA modifications in mammals, discuss their strengths and weaknesses, and speculate on the future direction of DNA modification profiling technologies.作者: brother 時(shí)間: 2025-3-22 23:54
Liquid Chromatography–Mass Spectrometry Analysis of Cytosine Modificationsced a similar rate of development and is often a bottleneck to chemical analysis. Here we present a protocol for identification and quantification of cytosine modifications that combines a robust and efficient method to generate single nucleosides from genomic DNA samples followed by direct LC-MS/MS analysis.作者: Outwit 時(shí)間: 2025-3-23 01:52
Book 2021A nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techn作者: Vaginismus 時(shí)間: 2025-3-23 06:12
Sihem Mesnager,Ferruh ?zbudak,Ahmet S?nakdroxymethyluracil (5hmU) and N6-methyladenine (6mA), and suggest potential explanations for inconsistencies in the currently available data on abundance and potential biological roles of these DNA modifications in mammals.作者: gerontocracy 時(shí)間: 2025-3-23 11:34 作者: gruelling 時(shí)間: 2025-3-23 17:15 作者: 詞根詞綴法 時(shí)間: 2025-3-23 19:07
Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Noncanonical DNA Basesthyl)-2′-deoxyuridine, correspondingly. Moreover, we provide optimized protocols for the oxidation of [.C.,.N.]-thymine to [.C.,.N.]-5-hydroxymethyluracil, [.C.,.N.]-5-formyluracil, and [.C.,.N.]-5-carboxyuracil using Na.S.O..作者: Mucosa 時(shí)間: 2025-3-24 01:23
1064-3745 expertsThis book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzym作者: Functional 時(shí)間: 2025-3-24 05:06 作者: FIG 時(shí)間: 2025-3-24 07:56 作者: Prognosis 時(shí)間: 2025-3-24 11:57
Berechnung von speziellen Funktionen,wo-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS) and high-performance liquid chromatography coupled with gas chromatography and mass spectrometry (HPLC/GC/MS).作者: PAGAN 時(shí)間: 2025-3-24 16:47
Pita J. C. Schimmelpenninck van der Oije lampbrush chromatin for immunostaining enables a straightforward approach to establishing the distributions of modified nucleotides within and between structurally and functionally distinctive chromatin domains.作者: 伸展 時(shí)間: 2025-3-24 22:00 作者: 精確 時(shí)間: 2025-3-25 02:03 作者: 幼稚 時(shí)間: 2025-3-25 04:11 作者: 好忠告人 時(shí)間: 2025-3-25 11:09
Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based ImmunocytochemistryC, 5-hydroxymethyluracil, 5hmU) in mammalian DNA. This method can be employed for evaluation of the levels and nuclear distribution of DNA modifications and permits their colocalization with protein markers in animal cells.作者: 間接 時(shí)間: 2025-3-25 12:13
https://doi.org/10.1007/978-1-0716-0876-0Non-canonical DNA nucleotides; DNA demethylation; Mass Spectrometry; Antibody-based techniques; Bioinfor作者: Mercantile 時(shí)間: 2025-3-25 17:11 作者: Endometrium 時(shí)間: 2025-3-25 20:28
Nusa Zidaric,Mark Aagaard,Guang Gongn Translocation (TET) dioxygenases oxidize 5mC to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine in metazoans and fungi. Moreover, two recent reports imply the existence of other species of modified cytosine in unicellular alga . and malaria parasite .. Here we provide an overview作者: MIRE 時(shí)間: 2025-3-26 00:38 作者: BUMP 時(shí)間: 2025-3-26 04:28 作者: 詞匯記憶方法 時(shí)間: 2025-3-26 12:33
Lecture Notes in Computer Scienceng is the availability of techniques that permit sensitive and specific genome-wide mapping of DNA modifications in mammalian DNA. The last decade has seen the development of a vast arsenal of novel profiling approaches enabling epigeneticists to tackle research questions that were previously out of作者: 傳授知識(shí) 時(shí)間: 2025-3-26 15:36
?etin Kaya Ko?,Sihem Mesnager,Erkay Sava?te. The continuing discovery of new modifications in phages suggest many more are waiting to be found. Thus, methods for the observation and characterization of noncanonical nucleosides are timely. We present here protocols for extraction of genomic DNA from bacteriophage particles, enzymatic hydrol作者: 熱心 時(shí)間: 2025-3-26 19:38 作者: 大暴雨 時(shí)間: 2025-3-27 00:21
Lecture Notes in Computer Sciencehylation have become increasingly more specific and sensitive over time. Conventional methods for detecting DNA methylation, ranging from paper chromatography to differential restriction enzyme digestion preference to dot blots, have more recently been supplemented by ultrahigh performance liquid ch作者: 外科醫(yī)生 時(shí)間: 2025-3-27 04:28 作者: 重畫只能放棄 時(shí)間: 2025-3-27 06:07 作者: DOTE 時(shí)間: 2025-3-27 12:09 作者: cunning 時(shí)間: 2025-3-27 14:26
https://doi.org/10.1007/978-3-031-45445-5 methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic stud作者: 遺產(chǎn) 時(shí)間: 2025-3-27 19:10
Titus Utibe,Ilufoye Sarafa Ogundiya 5-methylcytosine (5mC) in the zygote has led to the discovery that a global loss of DNA methylation takes place soon after fertilization, occurring rapidly in the paternal pronucleus. Using the same method employed above, which detects modified bases in the denatured single stranded DNA, we showed 作者: Expediency 時(shí)間: 2025-3-27 23:04
Pita J. C. Schimmelpenninck van der Oijed open chromatin structural domains to be directly observable by simple light microscopy. Moreover, the method described here for preparing spreads of lampbrush chromatin for immunostaining enables a straightforward approach to establishing the distributions of modified nucleotides within and betwee作者: badinage 時(shí)間: 2025-3-28 04:30 作者: 傲慢人 時(shí)間: 2025-3-28 07:43
Pita J. C. Schimmelpenninck van der Oijelevels is possible through use of immunohistochemistry. In this chapter we describe an adaptable method of brain tissue collection and immunohistochemical staining that allows for detection of 5hmC in mouse or rat brain, meaning that the method can be applied to many rodent models of CNS diseases an作者: 側(cè)面左右 時(shí)間: 2025-3-28 12:38 作者: kindred 時(shí)間: 2025-3-28 14:46 作者: Budget 時(shí)間: 2025-3-28 20:45
Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brainlevels is possible through use of immunohistochemistry. In this chapter we describe an adaptable method of brain tissue collection and immunohistochemical staining that allows for detection of 5hmC in mouse or rat brain, meaning that the method can be applied to many rodent models of CNS diseases and disorders.作者: 得體 時(shí)間: 2025-3-29 01:27 作者: 招致 時(shí)間: 2025-3-29 06:50
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260167.jpg作者: 遣返回國 時(shí)間: 2025-3-29 09:09
DNA Modifications978-1-0716-0876-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 在駕駛 時(shí)間: 2025-3-29 13:55
https://doi.org/10.1007/978-3-031-45445-5 methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic studies of TET.作者: Genistein 時(shí)間: 2025-3-29 19:03 作者: EWER 時(shí)間: 2025-3-29 22:40 作者: 失望未來 時(shí)間: 2025-3-30 03:39
Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overviewd its oxidized derivatives in the regulation of gene expression are relatively well attested and extensively explored, a number of recent studies imply that noncytosine DNA modifications may also convey specific biological functions and act as “epigenetic” marks in multicellular organisms. Here we r作者: GROUP 時(shí)間: 2025-3-30 05:59
Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and generated DNA nucleobase modifications. It is regarded as a gold standard for such analyses. Here, we consider the requirements for reliable identification and quantification of DNA adducts/modifications, whether endogenously derived or not, and discuss how their quantification can provide informat作者: Electrolysis 時(shí)間: 2025-3-30 09:15 作者: 從容 時(shí)間: 2025-3-30 14:44 作者: Decibel 時(shí)間: 2025-3-30 17:25 作者: obstinate 時(shí)間: 2025-3-30 22:48 作者: 異端邪說下 時(shí)間: 2025-3-31 03:30
Quantification of DNA Modifications Using Two-Dimensional Ultraperformance Liquid Chromatography Tanbolic processes. 8-oxoguanine, one of the oxidatively modified DNA bases is a typical biomarker of oxidative stress. A noncanonical base, uracil, may also be present in small quantities in DNA. Ten-eleven translocation (TET) proteins are involved in oxidation of 5-methylcytosine to 5-hydroxymethylcy作者: 特征 時(shí)間: 2025-3-31 07:13 作者: construct 時(shí)間: 2025-3-31 10:51
Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Noncanonical DNA Basesnternal standards. Only some of them are commercially available. Here we present a method allowing for the synthesis of [.C.,.N.]-5-methyl-2′-deoxycytidine from [.C.,.N.]-2′-deoxythymidine. We also describe an approach for the oxidation of [.C.,.N.]-5-methyl-2′-deoxycytidine and [.C.,.N.]-2′-deoxyth
