標(biāo)題: Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin [打印本頁(yè)] 作者: 生長(zhǎng)變吼叫 時(shí)間: 2025-3-21 18:22
書(shū)目名稱(chēng)DNA Cloning and Assembly影響因子(影響力)
書(shū)目名稱(chēng)DNA Cloning and Assembly影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)DNA Cloning and Assembly網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)DNA Cloning and Assembly網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)DNA Cloning and Assembly被引頻次
書(shū)目名稱(chēng)DNA Cloning and Assembly被引頻次學(xué)科排名
書(shū)目名稱(chēng)DNA Cloning and Assembly年度引用
書(shū)目名稱(chēng)DNA Cloning and Assembly年度引用學(xué)科排名
書(shū)目名稱(chēng)DNA Cloning and Assembly讀者反饋
書(shū)目名稱(chēng)DNA Cloning and Assembly讀者反饋學(xué)科排名
作者: Mast-Cell 時(shí)間: 2025-3-21 22:10
Generation of MoClo Standard Parts Using Golden Gate Cloning for amplification, (3) performing PCR amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large basic parts, it is preferable to first clone subparts as intermediate level??1 constructs. These subparts are sequenced individually and are作者: Affection 時(shí)間: 2025-3-22 01:23 作者: beta-carotene 時(shí)間: 2025-3-22 05:26
Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Sw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential. 作者: cauda-equina 時(shí)間: 2025-3-22 11:49
BASIC: A Simple and Accurate Modular DNA Assembly Methodenter a subsequent BASIC assembly without the need for reformatting or changes to the workflow. This unique idempotent cloning mechanism allows for the assembly of constructs in multiple, conceptionally simple hierarchical rounds. Combined with its high accuracy and robustness, this makes BASIC a ve作者: 燕麥 時(shí)間: 2025-3-22 13:51 作者: 燕麥 時(shí)間: 2025-3-22 18:42 作者: Hemiplegia 時(shí)間: 2025-3-23 00:35 作者: ABOUT 時(shí)間: 2025-3-23 03:06
Die ?konomische Sicht auf die Arbeitw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential. 作者: maverick 時(shí)間: 2025-3-23 07:01 作者: 充滿(mǎn)裝飾 時(shí)間: 2025-3-23 13:05
1064-3745 ader, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .DNA Cloning and Assembly: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-0716-0910-1978-1-0716-0908-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 手術(shù)刀 時(shí)間: 2025-3-23 15:46 作者: Duodenitis 時(shí)間: 2025-3-23 20:17
https://doi.org/10.1007/978-3-531-90438-2NGS libraries for pooled sequencing. This chapter describes a high-throughput protocol that enables the simultaneous sequencing of thousands of DNA constructs in a single sequencing run at a dramatically reduced cost compared to bench-top?methods.作者: 外來(lái) 時(shí)間: 2025-3-24 02:02
Computer — Mega-Maschine oder Werkzeug of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.作者: 燒瓶 時(shí)間: 2025-3-24 05:37
Highly Multiplexed, Semiautomated Nextera Next-Generation Sequencing (NGS) Library PreparationNGS libraries for pooled sequencing. This chapter describes a high-throughput protocol that enables the simultaneous sequencing of thousands of DNA constructs in a single sequencing run at a dramatically reduced cost compared to bench-top?methods.作者: 財(cái)主 時(shí)間: 2025-3-24 06:51
Hierarchical Modular DNA Assembly Using MetClo of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.作者: 變白 時(shí)間: 2025-3-24 13:08 作者: 干涉 時(shí)間: 2025-3-24 14:51 作者: 愚蠢人 時(shí)間: 2025-3-24 19:06 作者: 使混合 時(shí)間: 2025-3-24 23:14
https://doi.org/10.1007/978-3-658-36320-8he Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.作者: Gum-Disease 時(shí)間: 2025-3-25 04:47
Book 2020synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions 作者: Ophthalmologist 時(shí)間: 2025-3-25 10:51 作者: terazosin 時(shí)間: 2025-3-25 15:27
Orientierung an Gebrauchswertenthe structure of the final construct to identify all basic parts and vectors required for the construction strategy. The assembly strategy is then defined following a set of standard rules. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.作者: Diluge 時(shí)間: 2025-3-25 19:09 作者: padding 時(shí)間: 2025-3-25 20:46 作者: 突襲 時(shí)間: 2025-3-26 01:51
Assembly of Multigene Constructs Using the Modular Cloning System MoClothe structure of the final construct to identify all basic parts and vectors required for the construction strategy. The assembly strategy is then defined following a set of standard rules. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.作者: Pudendal-Nerve 時(shí)間: 2025-3-26 05:00
Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plantsdesign and construct new Phytobricks as well as how to assemble them in both manual and nanoscale automated one-step reactions. Finally, we describe a high-throughput method for sequence verification of assembled plasmids.作者: depreciate 時(shí)間: 2025-3-26 10:41
Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with s. Here we examine available DNA assembly methods and describe through example, the construction of a complex but not atypical combinatorial and hierarchical library using protocols that are generated automatically with the assistance of modern synthetic biology software.作者: analogous 時(shí)間: 2025-3-26 15:32 作者: Lamina 時(shí)間: 2025-3-26 19:29 作者: 勤勉 時(shí)間: 2025-3-27 00:29
Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Hosthe Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.作者: 假裝是你 時(shí)間: 2025-3-27 02:37
Book 2020mat, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .DNA Cloning and Assembly: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: 隱士 時(shí)間: 2025-3-27 08:51 作者: 頌揚(yáng)國(guó)家 時(shí)間: 2025-3-27 13:14
Forschung im Dialog — Einleitung are used to accelerate reaction setup, miniaturize reaction volumes, and dramatically reduce reagent and consumable cost. Although the focus is on generating DNA parts for use in DNA assembly techniques, this methodology can be applied to any workflow where the parallel production of hundreds or thousands of PCR amplicons is required.作者: FOLD 時(shí)間: 2025-3-27 17:08
Theorien industrieller Beziehungeng iterative design–build–test–learn (DBTL) cycles. In this book chapter, we highlight some of the bioCAD/CAM tools developed and used at the US Department of Energy (DOE) Joint Genome Institute (JGI), Joint BioEnergy Institute (JBEI), and Agile BioFoundry (ABF). We demonstrate the use of these bioCA作者: Synthesize 時(shí)間: 2025-3-27 21:43
über Produktivkr?fte und Bürgerrechteeactions that are easier to automate and work more reliably than traditional cloning methods. The simplicity of the chemistry is in contrast to the increased work needed to design optimal reactions that maximize DNA fragment reuse, minimize cost, and organize thousands of potential chemical reaction作者: Debility 時(shí)間: 2025-3-28 01:35 作者: conscribe 時(shí)間: 2025-3-28 04:31
Forschung im Dialog — Einleitung are used to accelerate reaction setup, miniaturize reaction volumes, and dramatically reduce reagent and consumable cost. Although the focus is on generating DNA parts for use in DNA assembly techniques, this methodology can be applied to any workflow where the parallel production of hundreds or th作者: 不易燃 時(shí)間: 2025-3-28 07:06 作者: WITH 時(shí)間: 2025-3-28 13:53 作者: Abrade 時(shí)間: 2025-3-28 15:53 作者: Intellectual 時(shí)間: 2025-3-28 21:53
Orientierung an Gebrauchswertento use, efficient, and allow users to assemble multigene constructs by performing a series of one-pot assembly steps, starting from libraries of cloned and sequenced parts. The efficiency of these systems also facilitates the generation of libraries of construct variants. We describe here a protocol作者: crease 時(shí)間: 2025-3-29 00:31
Computer — Mega-Maschine oder Werkzeugpe IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set作者: 碎片 時(shí)間: 2025-3-29 03:51 作者: 清澈 時(shí)間: 2025-3-29 09:13 作者: oxidize 時(shí)間: 2025-3-29 11:35 作者: Vertical 時(shí)間: 2025-3-29 16:17
Die ?konomische Sicht auf die Arbeitbly methods. Firstly, coding sequences are assembled with upstream and downstream sequences via overhangs corresponding to start and stop codons, avoiding unwanted ‘scars’ in assembled constructs at coding sequence boundaries. Scars at these crucial, sensitive locations can affect mRNA structure, ac作者: 人造 時(shí)間: 2025-3-29 22:17 作者: GILD 時(shí)間: 2025-3-30 01:49 作者: trigger 時(shí)間: 2025-3-30 05:01 作者: Repetitions 時(shí)間: 2025-3-30 12:07 作者: Jubilation 時(shí)間: 2025-3-30 13:56 作者: conjunctiva 時(shí)間: 2025-3-30 18:34 作者: AMOR 時(shí)間: 2025-3-30 22:28
