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標(biāo)題: Titlebook: DNA Arrays; Methods and Protocol Jang B. Rampal Book 2001 Humana Press 2001 [打印本頁]

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作者: 慢慢沖刷    時間: 2025-3-21 22:42
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260101.jpg
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https://doi.org/10.1007/978-94-011-8836-4fication and detection of intraspecies sequence variations, either between individuals or populations. The information drawn from those studies will lead to a detailed understanding of genetic and environmental contributions to the etiology of complex diseases.
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Gene Expression Analysis on Medium-Density Oligonucleotide Arrays,sequencing of their DNA, unique opportunities are becoming available for studying genetic variation in humans and its relationship with disease risk and aging. Consequently, new techniques have been invented to rapidly screen genes for biological information.
作者: 排名真古怪    時間: 2025-3-24 00:30
Aquinas’ Proofs for God’s Existencet. However readers could benefit from learning something about the technical basis of DNA microarrays, and younger readers may be curious to know something of the origins and antecedents of this new technology. In this chapter, I have attempted also a critical overview of the current state of the ar
作者: 旅行路線    時間: 2025-3-24 05:46
The Domain of Accident-Substanceogical information. The principle of the technology is based on specific molecular recognition interactions between the arrayed macromolecules and the test molecule of interest. Classical examples of such recognition reactions are the interactions between the two complementary strands of a double-he
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Rights and Obligations of the Partiestific concepts to research or clinical endeavors. Aside from obvious cases of scientific fraud and continuing controversies over natural selection and biological determinism, a long and varied history of eugenics movements grew out of the (re)discovery of Mendel’s experiments at the beginning of the
作者: 噱頭    時間: 2025-3-24 16:44
Formation of the Contract of Employmentl research and clinical diagnostics (.). These arrays are comprised of large sets of nucleic acid probe sequences immobilized in defined, addressable locations on the surface of a substrate, and are capable of acquiring unprecedented amounts of genetic information from biological samples in a single
作者: TOXIC    時間: 2025-3-24 21:19
https://doi.org/10.1007/978-94-011-8836-4fication and detection of intraspecies sequence variations, either between individuals or populations. The information drawn from those studies will lead to a detailed understanding of genetic and environmental contributions to the etiology of complex diseases.
作者: Density    時間: 2025-3-25 01:26
Formation of the Contract of Employmentthe past decade (.,.). In addition to the high density and/or small area achieved by using microspot arrays, increased sensitivity is possible for some applications (.). Ink-jet printing technology has been used to produce microspot arrays of both DNA and antibodies (.), and the procedure to create
作者: 煞費(fèi)苦心    時間: 2025-3-25 06:11
Formation of the Contract of Employmentrs of DNA samples (either complete cDNAs or ESTs) are immobilized as very high-density arrays on solid surfaces, typically glass. Microarray analysis is conceptually similar to that of a reverse dot blot, whereby the tethered “probe” DNA samples comprising the array are hybridized with fluorescently
作者: ATRIA    時間: 2025-3-25 09:12
https://doi.org/10.1007/978-94-017-6306-6nally developed by physicists and applied to biology is the microchip. The semiconductor industry manufactures silica chips with increasing numbers of smaller and smaller features, which allows incredible numbers of operations within split seconds. The same principle of performing multiparallel oper
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https://doi.org/10.1057/9781137551412 mRNA and inhibit translation. AONs show promise as therapeutics to many diseases caused by abnormal or unwanted expression of a gene, such as cancers. The first antisense oligonucleotide-based drug (Vitravene?: ISIS Pharmaceuticals) was introduced in 1998 to treat cytomegalovirus (CMV) retinitis in
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https://doi.org/10.1057/9780230376540sequencing of their DNA, unique opportunities are becoming available for studying genetic variation in humans and its relationship with disease risk and aging. Consequently, new techniques have been invented to rapidly screen genes for biological information.
作者: Painstaking    時間: 2025-3-26 11:41
The Genesis of Arab Nationalismxt step is to scan in the glass, gel, or plastic slides with a specialized scanner to obtain digital images of the results of the experiment. The DNA expression levels are then quantified with the help of image-analysis software. After the image processing and analysis step is completed, we end up w
作者: Tractable    時間: 2025-3-26 15:05
The Genesis of Arab Nationalismies of two-dimensional confocal slices of the specimen at different focus positions. Additionally, confocal microscopes are excellent for measuring weak fluorescence from areas adjacent to areas of very strong fluorescence, because the confocal pinhole rejects light from areas outside the focus spot
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The Genesis of Arab Nationalismtionality, or both. Microfluidic circuitry has yielded the lab-on-a-chip concept in which functions such as sample processing, reagent combining, component separation, and detection all occur in sequence on microscale integrated devices. Highly parallel functionality manifests in DNA microarrays, wh
作者: 無畏    時間: 2025-3-26 22:22
Book 2001at it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility
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作者: 注視    時間: 2025-3-27 08:21
Sequencing by Hybridization Arrays, second of these processes: the use of sequence-specific hybridization of oligonucleotide probes of known sequence to determine primary DNA structure. Refined over the past decade, such sequencing-by-hybridization (SBH) methods have become important tools in the field of genomics research.
作者: choleretic    時間: 2025-3-27 10:00
Low-Resolution Typing of , Using DNA Microarray,he short arm of chromosome 6. The area comprises three class regions: class I, which includes ., and . genes; class II, containing the . genes; and class III, composed of numerous protein coding genes responsible for a range of functions (.,.).
作者: Hiatal-Hernia    時間: 2025-3-27 14:38
Formation of the Contract of Employmentprobe sequences on a glass “chip” about 1.6 cm.. This method is used to produce high-density GeneChip? probe arrays, which are now finding widespread use in the detection and analysis of mutations and polymorphisms (“genotyping”), and in a wide range of gene expression studies.
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作者: 音樂等    時間: 2025-3-27 23:33
Photolithographic Synthesis of High-Density Oligonucleotide Arrays,probe sequences on a glass “chip” about 1.6 cm.. This method is used to produce high-density GeneChip? probe arrays, which are now finding widespread use in the detection and analysis of mutations and polymorphisms (“genotyping”), and in a wide range of gene expression studies.
作者: 指數(shù)    時間: 2025-3-28 05:01
Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays, chips or microarrays are miniaturized versions of these classical filter-hybridization techniques. With great precision, thousands of DNA fragments or DNA oligonucleotides can be printed onto glass chips or microscope slides. In the case of oligonucleotides, they can also be synthesized directly on coated silica chips.
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Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules,e applications (.). Ink-jet printing technology has been used to produce microspot arrays of both DNA and antibodies (.), and the procedure to create oligonucleotide microspot arrays is described in this chapter.
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https://doi.org/10.1007/978-94-010-2380-1 second of these processes: the use of sequence-specific hybridization of oligonucleotide probes of known sequence to determine primary DNA structure. Refined over the past decade, such sequencing-by-hybridization (SBH) methods have become important tools in the field of genomics research.
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DNA Microarrays,t. However readers could benefit from learning something about the technical basis of DNA microarrays, and younger readers may be curious to know something of the origins and antecedents of this new technology. In this chapter, I have attempted also a critical overview of the current state of the ar
作者: CORD    時間: 2025-3-30 04:54
Gel-Immobilized Microarrays of Nucleic Acids and Proteins,ogical information. The principle of the technology is based on specific molecular recognition interactions between the arrayed macromolecules and the test molecule of interest. Classical examples of such recognition reactions are the interactions between the two complementary strands of a double-he
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作者: 不能和解    時間: 2025-3-30 12:22
Ethical Ramifications of Genetic Analysis Using DNA Arrays,tific concepts to research or clinical endeavors. Aside from obvious cases of scientific fraud and continuing controversies over natural selection and biological determinism, a long and varied history of eugenics movements grew out of the (re)discovery of Mendel’s experiments at the beginning of the
作者: Organonitrile    時間: 2025-3-30 19:33
Photolithographic Synthesis of High-Density Oligonucleotide Arrays,l research and clinical diagnostics (.). These arrays are comprised of large sets of nucleic acid probe sequences immobilized in defined, addressable locations on the surface of a substrate, and are capable of acquiring unprecedented amounts of genetic information from biological samples in a single
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作者: Prosaic    時間: 2025-3-31 02:13
Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules,the past decade (.,.). In addition to the high density and/or small area achieved by using microspot arrays, increased sensitivity is possible for some applications (.). Ink-jet printing technology has been used to produce microspot arrays of both DNA and antibodies (.), and the procedure to create
作者: 高歌    時間: 2025-3-31 09:04
,Printing DNA Microarrays Using the Biomek? 2000 Laboratory Automation Workstation,rs of DNA samples (either complete cDNAs or ESTs) are immobilized as very high-density arrays on solid surfaces, typically glass. Microarray analysis is conceptually similar to that of a reverse dot blot, whereby the tethered “probe” DNA samples comprising the array are hybridized with fluorescently
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DNA Sequencing by Hybridization with Arrays of Samples or Probes,NA sequence information by screening DNA oligomers (typically 7-to 11-mers) for their ability to hybridize with target DNA. The set of overlapping oligomers that matches the target DNA is then used to assemble its sequence. The theory, practice, and history of SBH are reviewed in .. and ..
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Use of Bioinformatics in Arrays,xt step is to scan in the glass, gel, or plastic slides with a specialized scanner to obtain digital images of the results of the experiment. The DNA expression levels are then quantified with the help of image-analysis software. After the image processing and analysis step is completed, we end up w
作者: 富饒    時間: 2025-4-1 15:26

作者: 貧窮地活    時間: 2025-4-1 21:50
Business Aspects of Biochip Technologies,tionality, or both. Microfluidic circuitry has yielded the lab-on-a-chip concept in which functions such as sample processing, reagent combining, component separation, and detection all occur in sequence on microscale integrated devices. Highly parallel functionality manifests in DNA microarrays, wh




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