標(biāo)題: Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi [打印本頁] 作者: ACRO 時(shí)間: 2025-3-21 17:26
書目名稱cAMP Signaling影響因子(影響力)
書目名稱cAMP Signaling影響因子(影響力)學(xué)科排名
書目名稱cAMP Signaling網(wǎng)絡(luò)公開度
書目名稱cAMP Signaling網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱cAMP Signaling被引頻次
書目名稱cAMP Signaling被引頻次學(xué)科排名
書目名稱cAMP Signaling年度引用
書目名稱cAMP Signaling年度引用學(xué)科排名
書目名稱cAMP Signaling讀者反饋
書目名稱cAMP Signaling讀者反饋學(xué)科排名
作者: dendrites 時(shí)間: 2025-3-21 22:39
Daniel Carvalho,Telmo Silva,Jorge Abreully performed in vitro using radio-labeled ATP. For in vivo studies, genetically encoded FRET-based sensors have become popular for monitoring PKA activity. Here, we show that it is also possible to apply such reporters in vitro. We describe how to express and purify milligram quantities of a FRET-b作者: 字形刻痕 時(shí)間: 2025-3-22 01:07 作者: Painstaking 時(shí)間: 2025-3-22 07:40
Applications and Usability of Interactive TVgenetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronal function by different approaches. In this chapter we describe the use of photoactivated adenylyl cyclases (PACs) as modulators of ne作者: Microaneurysm 時(shí)間: 2025-3-22 08:58 作者: 換話題 時(shí)間: 2025-3-22 15:42
Applications and Usability of Interactive TVith PKA-repressed reporters to either carry out high throughput screens for small molecule inhibitors of these target enzymes or to assess hit compounds and their analogs from such screens. Here, we describe two methods for testing panels of such compounds. The first uses a growth assay for which gr作者: 換話題 時(shí)間: 2025-3-22 19:13 作者: vertebrate 時(shí)間: 2025-3-22 21:51 作者: 侵略 時(shí)間: 2025-3-23 05:25
Carina Haro Granizo,Gonzalo Olmedoiomyocytes. However, as the differentiation process is lengthy and commercially available cells are expensive, the cell number is limited. Here we provide detailed information on how to scale down 2D cell cultures of hIPS-CMs for the purpose of cAMP FRET measurements, thereby extending the number of作者: 常到 時(shí)間: 2025-3-23 06:46 作者: nascent 時(shí)間: 2025-3-23 12:34 作者: 高興一回 時(shí)間: 2025-3-23 15:36
David Campelo,Telmo Silva,Jorge Abreug cardiomyocyte function, as well as pathological processes, by acting in distinct subcellular microdomains and thus controlling excitation–contraction coupling. Spatio-temporal intracellular dynamics of cyclic nucleotides can be measured in living cells using fluorescence resonance energy transfer 作者: endoscopy 時(shí)間: 2025-3-23 18:44 作者: 群居動(dòng)物 時(shí)間: 2025-3-23 23:21
https://doi.org/10.1007/978-3-319-90170-1xicity or no autofluorescence, and compatibility to deep-tissue imaging or optogenetics. However, functional imaging of cellular signaling by bioluminescence is not so easy due to the limited availability of bright bioluminescent indicators..Here we describe a detailed strategy to detect cellular cA作者: Cognizance 時(shí)間: 2025-3-24 03:04
https://doi.org/10.1007/978-3-319-90170-1nsgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid 作者: Anthrp 時(shí)間: 2025-3-24 07:33
David Campelo,Telmo Silva,Jorge Abreuing of physiological and pathological processes. However, the development of sensors remains an intricate process. Using simulation techniques, we recently introduced a new architecture to measure intracellular concentrations of cAMP named CUTie, which works as a FRET tag for arbitrary targeting dom作者: 宣稱 時(shí)間: 2025-3-24 13:00
Michele Roncalli,Alessandro Farinelliat have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here, we describe two complementary approaches for the detection and measurement of cAMP signals near the plasm作者: 付出 時(shí)間: 2025-3-24 17:15
Smart Cities for the Rest of Usion of key target proteins via activation of the cAMP effector protein kinase A (PKA) is achieved via signal compartmentalization. Termination of the cAMP signal is mediated by phosphodiesterases (PDEs), a diverse group of enzymes comprising several families that localize to distinct cellular compar作者: Boycott 時(shí)間: 2025-3-24 20:08
Smart Cities for the Rest of Us), a Ser/Thr protein kinase. Where and when this enzyme is activated inside the cell has profound implications on the functional impact of PKA. It is now well established that PKA signaling is focused locally into subcellular signaling “islands” or “signalosomes.” The A-Kinase Anchoring Proteins (AK作者: CONE 時(shí)間: 2025-3-25 01:09 作者: 名義上 時(shí)間: 2025-3-25 07:20 作者: 是突襲 時(shí)間: 2025-3-25 08:35 作者: 討好女人 時(shí)間: 2025-3-25 12:28
Manuela ZaccoloIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Minikin 時(shí)間: 2025-3-25 16:49 作者: incredulity 時(shí)間: 2025-3-25 21:27 作者: Radiculopathy 時(shí)間: 2025-3-26 02:00
https://doi.org/10.1007/978-3-030-23862-9lls using targeted genetically encoded FRET biosensors. Key experimental difficulties include gathering sufficient signal from such a small, photon-limited volume, and the susceptibility of cilia to movement artifacts. Other challenges are associated with the fidelity of sensor targeting and the dif作者: MUTE 時(shí)間: 2025-3-26 04:46
Carina Haro Granizo,Gonzalo Olmedon interactions. In part, this is due to the lack of agents for specifically targeting defined protein–protein interactions. Peptidic and non-peptidic inhibitors are invaluable molecular tools for elucidating the functions of AKAP-dependent protein–protein interactions. In addition, such interaction 作者: 編輯才信任 時(shí)間: 2025-3-26 08:28
Carina Haro Granizo,Gonzalo Olmedo FRET measurements with this sensor and the analysis of generated imaging data. Numbers for seeding areas, seeding densities, coating volumes and concentrations, media volumes, and concentrations of reagents are given as guidelines.作者: 不易燃 時(shí)間: 2025-3-26 13:39 作者: cognizant 時(shí)間: 2025-3-26 19:59 作者: 不幸的人 時(shí)間: 2025-3-26 23:59 作者: Presbycusis 時(shí)間: 2025-3-27 01:21 作者: instructive 時(shí)間: 2025-3-27 07:46
Imaging the cAMP Signaling Microdomain of the Primary Cilium Using Targeted FRET-Based Biosensors,lls using targeted genetically encoded FRET biosensors. Key experimental difficulties include gathering sufficient signal from such a small, photon-limited volume, and the susceptibility of cilia to movement artifacts. Other challenges are associated with the fidelity of sensor targeting and the dif作者: macabre 時(shí)間: 2025-3-27 10:54
,Disruptors of AKAP-Dependent Protein–Protein Interactions,n interactions. In part, this is due to the lack of agents for specifically targeting defined protein–protein interactions. Peptidic and non-peptidic inhibitors are invaluable molecular tools for elucidating the functions of AKAP-dependent protein–protein interactions. In addition, such interaction 作者: 無節(jié)奏 時(shí)間: 2025-3-27 14:16
Micro-2D Cell Culture for cAMP Measurements Using FRET Reporters in Human iPSC-Derived Cardiomyocyt FRET measurements with this sensor and the analysis of generated imaging data. Numbers for seeding areas, seeding densities, coating volumes and concentrations, media volumes, and concentrations of reagents are given as guidelines.作者: anagen 時(shí)間: 2025-3-27 20:44 作者: 鬧劇 時(shí)間: 2025-3-28 00:57
How to Make the CUTiest Sensor in Three Simple Steps for Computational Pedestrians,60?nM)..This simple protocol, which integrates our previous experience, only requires free web servers and can be straightforwardly used to create cAMP sensors adapted to the physicochemical characteristics of known cyclic nucleotide-binding domains.作者: Flirtatious 時(shí)間: 2025-3-28 04:32
Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes,. The latter approach evaluates the composition of PKA holoenzymes, in which regulatory subunits and catalytic subunits can be visualized in the presence of test compounds and small-molecule inhibitors.作者: misanthrope 時(shí)間: 2025-3-28 10:12 作者: FLAG 時(shí)間: 2025-3-28 11:40 作者: heartburn 時(shí)間: 2025-3-28 15:07
Real-Time Measurements of Intracellular cAMP Gradients Using FRET-Based cAMP Nanorulers,eptors (GPCRs). In a single cell, cAMP can exert innumerous specific cell functions in response to more than one hundred different GPCRs. Cells achieve this extraordinary functional specificity of cAMP signaling by limiting the spread of?these signals in space and time. To do so, cells establish nan作者: 褲子 時(shí)間: 2025-3-28 22:00
Assaying Protein Kinase A Activity Using a FRET-Based Sensor Purified from Mammalian Cells,lly performed in vitro using radio-labeled ATP. For in vivo studies, genetically encoded FRET-based sensors have become popular for monitoring PKA activity. Here, we show that it is also possible to apply such reporters in vitro. We describe how to express and purify milligram quantities of a FRET-b作者: glisten 時(shí)間: 2025-3-29 00:18
MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET,n. Here we describe a detailed protocol for setup and use of this software for any purpose requiring instant feedback during fluorescence measurement experiments. We further describe its non-primary features including beam splitter misalignment correction, custom calculations through input of simple作者: Budget 時(shí)間: 2025-3-29 06:25
Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity,genetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronal function by different approaches. In this chapter we describe the use of photoactivated adenylyl cyclases (PACs) as modulators of ne作者: 僵硬 時(shí)間: 2025-3-29 10:55 作者: meritorious 時(shí)間: 2025-3-29 15:22 作者: 團(tuán)結(jié) 時(shí)間: 2025-3-29 19:21
Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors,sensors are ideal to visualize and measure the often rapid changes of second messenger concentrations in time and place. Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative technique for measuring FRET. Given the recent development of commercially available, sensitive and photon-ef作者: 牛的細(xì)微差別 時(shí)間: 2025-3-29 23:11 作者: CRP743 時(shí)間: 2025-3-30 03:46
Micro-2D Cell Culture for cAMP Measurements Using FRET Reporters in Human iPSC-Derived Cardiomyocytiomyocytes. However, as the differentiation process is lengthy and commercially available cells are expensive, the cell number is limited. Here we provide detailed information on how to scale down 2D cell cultures of hIPS-CMs for the purpose of cAMP FRET measurements, thereby extending the number of作者: overrule 時(shí)間: 2025-3-30 07:30
Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification,ch to measure localized cAMP signals. However, given the low signal-to-noise ratio of most FRET probes and the dynamic nature of the intracellular environment, there have been marked limitations in the ability to use FRET probes to study localized signaling events within the same cell. Here, we outl作者: Explosive 時(shí)間: 2025-3-30 11:21 作者: apiary 時(shí)間: 2025-3-30 14:38 作者: Antecedent 時(shí)間: 2025-3-30 17:14 作者: 松軟 時(shí)間: 2025-3-30 23:47 作者: 誓言 時(shí)間: 2025-3-31 01:49
Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP,nsgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid 作者: 和音 時(shí)間: 2025-3-31 09:01 作者: 漸強(qiáng) 時(shí)間: 2025-3-31 09:19
,Ion Channel–Based Reporters for cAMP Detection,at have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here, we describe two complementary approaches for the detection and measurement of cAMP signals near the plasm作者: Gourmet 時(shí)間: 2025-3-31 14:42
Quantitative Phosphoproteomics to Study cAMP Signaling,ion of key target proteins via activation of the cAMP effector protein kinase A (PKA) is achieved via signal compartmentalization. Termination of the cAMP signal is mediated by phosphodiesterases (PDEs), a diverse group of enzymes comprising several families that localize to distinct cellular compar作者: Expediency 時(shí)間: 2025-3-31 19:38 作者: 切割 時(shí)間: 2025-3-31 22:33
María J. Abásolo,Jorge Abreu,Telmo Silva equations typed in a .txt format, customizable Excel output, and offline bulk analysis of image stacks. Finally, we supply a usage example of a cAMP measurement in cultured rat neonatal cardiomyocytes.作者: 僵硬 時(shí)間: 2025-4-1 02:00
Applications and Usability of Interactive TVowth in medium containing the pyrimidine analog 5-fluoro orotic acid (5FOA) occurs in response to inhibiting PDE activity to activate PKA. The second uses mass spectrometry to directly measure the impact of compound treatment to study compounds that modulate either PDE or AC activity.作者: 沉默 時(shí)間: 2025-4-1 07:34
