標(biāo)題: Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2015 Springer Science+Business Media New York 2015 Adenosine 3′,5′-monophosphate. [打印本頁] 作者: 可入到 時(shí)間: 2025-3-21 19:48
書目名稱cAMP Signaling影響因子(影響力)
書目名稱cAMP Signaling影響因子(影響力)學(xué)科排名
書目名稱cAMP Signaling網(wǎng)絡(luò)公開度
書目名稱cAMP Signaling網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱cAMP Signaling被引頻次
書目名稱cAMP Signaling被引頻次學(xué)科排名
書目名稱cAMP Signaling年度引用
書目名稱cAMP Signaling年度引用學(xué)科排名
書目名稱cAMP Signaling讀者反饋
書目名稱cAMP Signaling讀者反饋學(xué)科排名
作者: 僵硬 時(shí)間: 2025-3-21 23:22
Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging,wnstream cellular effectors. FRET-based sensors are ideal to visualize and measure these rapid changes of second messenger concentrations in time and place. Here, we describe the use of EPAC-based FRET sensors to measure cAMP with spatiotemporal resolution in cells by fluorescence lifetime imaging (作者: TIA742 時(shí)間: 2025-3-22 03:59
A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based cAMP) signaling and compartmentalization in living cells. These sensors allow estimation of relative changes of cAMP levels in real-time and intact cells. However, one of their major shortcomings is that they do not easily allow direct measurement of cAMP concentrations. This is mainly due to the fa作者: 手銬 時(shí)間: 2025-3-22 06:48 作者: 施魔法 時(shí)間: 2025-3-22 10:46 作者: FLUSH 時(shí)間: 2025-3-22 13:20
Channel-Based Reporters for cAMP Detection,hat have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here we describe two complementary approaches for the detection and measurement of cAMP signals near the plasm作者: FLUSH 時(shí)間: 2025-3-22 17:26
Imaging Sub-plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters,rs, a large number of tools for cAMP measurements have been developed. While most cAMP reporters are designed to undergo changes in fluorescence resonance energy transfer (FRET), there are alternative techniques with advantages for certain applications. Here, we describe protocols for cAMP measureme作者: evasive 時(shí)間: 2025-3-22 23:34 作者: 噱頭 時(shí)間: 2025-3-23 04:39 作者: hermetic 時(shí)間: 2025-3-23 06:45 作者: BINGE 時(shí)間: 2025-3-23 10:54 作者: 厭食癥 時(shí)間: 2025-3-23 15:51 作者: fatty-acids 時(shí)間: 2025-3-23 21:46
Structure-Based Bacteriophage Screening for AKAP-Selective PKA Regulatory Subunit Variants,elices that bind to the docking and dimerization (D/D) domain of PKA regulatory subunits. Peptide disruptors derived from AKAP anchoring helices are powerful tools for determining whether PKA anchoring is important in different biological processes. Focusing on the reciprocal side of the AKAP-PKA in作者: 侵蝕 時(shí)間: 2025-3-24 02:07 作者: inhumane 時(shí)間: 2025-3-24 02:55 作者: 縮減了 時(shí)間: 2025-3-24 09:00 作者: ALLAY 時(shí)間: 2025-3-24 10:50 作者: 船員 時(shí)間: 2025-3-24 16:57 作者: Employee 時(shí)間: 2025-3-24 19:18 作者: 粗魯性質(zhì) 時(shí)間: 2025-3-24 23:17 作者: entice 時(shí)間: 2025-3-25 05:41
1064-3745 chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting an978-1-4939-4950-2978-1-4939-2537-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 干涉 時(shí)間: 2025-3-25 08:57
https://doi.org/10.1007/978-3-031-22210-8lls stimulated with glucose and gluco-incretin hormones. We also demonstrate how the technique can be combined with measurements of the cytosolic Ca. concentration or with recordings of the subcellular localization of the cAMP effector protein Epac2. The translocation reporter approach provides a va作者: 樹上結(jié)蜜糖 時(shí)間: 2025-3-25 12:23
Applications and Usability of Interactive TViovascular, neuronal, and inflammatory diseases, but it is unclear whether these interactions are suitable drug targets. Here we describe an enzyme-linked immunosorbent assay (ELISA) for the screening of small molecule libraries for inhibitors of AKAP–PKA interactions. In addition, we describe a hom作者: FRET 時(shí)間: 2025-3-25 16:46
Marc Aguilar,Pau Pamplona,Sergi FernándezPKG and their associated proteins. Unfortunately, both PKA and PKG are enriched in the pull downs with both immobilized compounds. Although this proved sufficient to identify novel AKAPs, the lower abundance of PKG has seriously hampered the enrichment and identification of novel GKAPs. Here we pres作者: 斜坡 時(shí)間: 2025-3-25 23:42 作者: optic-nerve 時(shí)間: 2025-3-26 00:42 作者: Gum-Disease 時(shí)間: 2025-3-26 04:34 作者: 下邊深陷 時(shí)間: 2025-3-26 11:11
Adenoviral Transduction of FRET-Based Biosensors for cAMP in Primary Adult Mouse Cardiomyocytes,ts a powerful combination for the study of cAMP signaling in live primary cardiomyocytes. In this chapter, we describe the steps required during the isolation, viral transduction, and culture of cardiomyocytes from an adult mouse to obtain reliable expression of genetically encoded FRET biosensors for the study of cAMP signaling in living cells.作者: Permanent 時(shí)間: 2025-3-26 13:09
Photoactivatable Adenylyl Cyclases (PACs) as a Tool to Study cAMP Signaling In Vivo: An Overview,ols provide cyclase activity capable of increasing cellular cAMP levels up to a hundredfold with either . or . kinetic characteristics. Here, we consider the functional features of different cyclases and provide operating guidelines to optimize the use of PACs in vivo.作者: 清醒 時(shí)間: 2025-3-26 19:33 作者: 被告 時(shí)間: 2025-3-27 00:44 作者: 幻影 時(shí)間: 2025-3-27 05:03 作者: FUME 時(shí)間: 2025-3-27 05:53 作者: 聲明 時(shí)間: 2025-3-27 10:37
María José Abásolo,Raoni Kuleszasfer (FRET)-based biosensor for the second messenger 3′,5′-cyclic adenosine monophosphate. These tools and techniques should provide great potential for the analysis of second messenger dynamics in a more physiologically relevant context.作者: 愉快嗎 時(shí)間: 2025-3-27 17:05
Applications and Usability of Interactive TVng are valuable tools in the investigation of local PKA action. This article outlines the development of PKA isoform-selective disruptor peptides, documents the optimization of cell-soluble peptide derivatives, and introduces alternative cell-based approaches that interrogate other aspects of the PKA–AKAP interface.作者: countenance 時(shí)間: 2025-3-27 19:43
https://doi.org/10.1007/978-3-319-38907-3re for identifying variants of PKA regulatory subunit D/D domains that bind selectively to individual AKAPs. This procedure can be adapted for engineering specificity into other shared protein interfaces.作者: FORGO 時(shí)間: 2025-3-28 01:26
Jorge Abreu,Pedro Almeida,Telmo Silvaf cAMP computational models. We describe how features of the cAMP network can be represented and review the types of experimental data useful in modeling cAMP signaling. We also compile a list of published cAMP models that can aid in the development of novel dynamical models of cAMP signaling.作者: MEET 時(shí)間: 2025-3-28 03:28
Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-BaseAMP and its actions in subcellular compartments of neighboring cells. As proof of principle, we compare cAMP signals and PKA activity in the cytosol of neighboring HEK cells. We propose that this flexible and powerful method can significantly improve the direct comparison of cAMP signals and their action in specific cellular domains.作者: chandel 時(shí)間: 2025-3-28 09:12
Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters,aw simple qualitative rules that may aid the design of novel sensors. Finally, we will illustrate how the application of these simple rules can be used to describe the mechanistic basis of cAMP sensors reported in the literature.作者: 放大 時(shí)間: 2025-3-28 12:13 作者: Plaque 時(shí)間: 2025-3-28 16:32 作者: Negligible 時(shí)間: 2025-3-28 18:53 作者: biosphere 時(shí)間: 2025-3-29 00:38
Development of Computational Models of cAMP Signaling,f cAMP computational models. We describe how features of the cAMP network can be represented and review the types of experimental data useful in modeling cAMP signaling. We also compile a list of published cAMP models that can aid in the development of novel dynamical models of cAMP signaling.作者: 故意釣到白楊 時(shí)間: 2025-3-29 03:51 作者: Fierce 時(shí)間: 2025-3-29 10:15 作者: 秘密會(huì)議 時(shí)間: 2025-3-29 12:21
Applications and Usability of Interactive TVine a methodology to dissect kinetics of cAMP-mediated FRET signals in single cells using automated image analysis approaches. We additionally extend these approaches to the analysis of subcellular regions. These approaches offer an unique opportunity to assess localized cAMP kinetics in an unbiased, quantitative fashion.作者: circumvent 時(shí)間: 2025-3-29 16:47
https://doi.org/10.1007/978-3-031-22210-8a membrane of cells. These probes take advantage of the ability of cyclic nucleotide-gated (CNG) channels to transduce small changes in cAMP concentrations into ionic flux through channel pores that can be readily detected by measuring Ca. and/or Mn. influx or by measuring ionic currents.作者: 轎車 時(shí)間: 2025-3-29 23:39 作者: muster 時(shí)間: 2025-3-30 01:27
Applications and Usability of Interactive TVols provide cyclase activity capable of increasing cellular cAMP levels up to a hundredfold with either . or . kinetic characteristics. Here, we consider the functional features of different cyclases and provide operating guidelines to optimize the use of PACs in vivo.作者: dura-mater 時(shí)間: 2025-3-30 04:38
Marc Aguilar,Pau Pamplona,Sergi Fernándezfrom growing in medium containing the pyrimidine analog 5-fluoro orotic acid (5FOA). We describe here the steps required to construct strains for screening and to optimize conditions for successful screens.作者: Allergic 時(shí)間: 2025-3-30 09:39
https://doi.org/10.1007/978-1-4939-2537-7Adenosine 3′,5′-monophosphate; Cellular function; Disease mechanisms; Protein kinase A (PKA); Signaling 作者: 親屬 時(shí)間: 2025-3-30 13:48
978-1-4939-4950-2Springer Science+Business Media New York 2015作者: 與野獸博斗者 時(shí)間: 2025-3-30 18:23 作者: 幼兒 時(shí)間: 2025-3-31 00:41 作者: 拘留 時(shí)間: 2025-3-31 04:41
https://doi.org/10.1007/978-3-319-63321-3A), can be measured in living cells, thanks to genetically encoded probes based on fluorescence resonance energy transfer (FRET). While these biosensors enable the assessment of cAMP or PKA activity with great spatial and temporal resolution, concomitant events triggered by the same stimuli at the s作者: ovation 時(shí)間: 2025-3-31 05:54
Bernardo Cardoso,Jorge Ferraz de Abreuwnstream cellular effectors. FRET-based sensors are ideal to visualize and measure these rapid changes of second messenger concentrations in time and place. Here, we describe the use of EPAC-based FRET sensors to measure cAMP with spatiotemporal resolution in cells by fluorescence lifetime imaging (作者: fibula 時(shí)間: 2025-3-31 12:21
Applications and Usability of Interactive TVcAMP) signaling and compartmentalization in living cells. These sensors allow estimation of relative changes of cAMP levels in real-time and intact cells. However, one of their major shortcomings is that they do not easily allow direct measurement of cAMP concentrations. This is mainly due to the fa作者: 改變 時(shí)間: 2025-3-31 14:31
Applications and Usability of Interactive TV of local concentrations of second messengers in living cells. Nowadays, the availability of a number of 3D structures of cyclic nucleotide-binding domains (CNBD) undergoing conformational transitions upon cAMP binding, along with computational tools, can be exploited for the design of novel or impr作者: TERRA 時(shí)間: 2025-3-31 20:50
Applications and Usability of Interactive TVch to measure localized cAMP signals. However, given the low signal-to-noise ratio of most FRET probes and the dynamic nature of the intracellular environment, there have been marked limitations in the ability to use FRET probes to study localized signaling events within the same cell. Here, we outl作者: altruism 時(shí)間: 2025-4-1 00:02 作者: 涂掉 時(shí)間: 2025-4-1 02:20
https://doi.org/10.1007/978-3-031-22210-8rs, a large number of tools for cAMP measurements have been developed. While most cAMP reporters are designed to undergo changes in fluorescence resonance energy transfer (FRET), there are alternative techniques with advantages for certain applications. Here, we describe protocols for cAMP measureme
