標題: Titlebook: c-di-GMP Signaling; Methods and Protocol Karin Sauer Book 2017 Springer Science+Business Media LLC 2017 Pseudomonas aeruginosa.FimA.HPLC.c- [打印本頁] 作者: otitis-externa 時間: 2025-3-21 17:43
書目名稱c-di-GMP Signaling影響因子(影響力)
書目名稱c-di-GMP Signaling影響因子(影響力)學科排名
書目名稱c-di-GMP Signaling網(wǎng)絡公開度
書目名稱c-di-GMP Signaling網(wǎng)絡公開度學科排名
書目名稱c-di-GMP Signaling被引頻次
書目名稱c-di-GMP Signaling被引頻次學科排名
書目名稱c-di-GMP Signaling年度引用
書目名稱c-di-GMP Signaling年度引用學科排名
書目名稱c-di-GMP Signaling讀者反饋
書目名稱c-di-GMP Signaling讀者反饋學科排名
作者: Gustatory 時間: 2025-3-21 20:32
Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinationsed with phosphodiesterase activity. However, experimental confirmation of enzymatic activity is still necessary, as many proteins contain degenerate domains that lack catalytic activity but nonetheless function as c-di-GMP receptors.作者: Flagging 時間: 2025-3-22 03:24
Identification and Quantification of Cyclic Di-Guanosine Monophosphate and Its Linear Metabolites byG) and 5′-guanosine monophosphate (5′-GMP), as well as cyclic guanosine monophosphate (3′,5′-cGMP) and 3′,3′ c-di-GMP analogues (2′,3′-c-di-GMP and 2′,2′-c-di-GMP) can be simultaneously determined by this method.作者: 偏狂癥 時間: 2025-3-22 07:15 作者: GUILE 時間: 2025-3-22 12:04
Congo Red Stain Identifies Matrix Overproduction and Is an Indirect Measurement for c-di-GMP in Manyg of samples can be utilized as an indirect measurement of elevated c-di-GMP production in bacteria. Congo red allows the identification of strains producing high c-di-GMP in an inexpensive, quantitative, and high-throughput manner.作者: Atmosphere 時間: 2025-3-22 14:04 作者: Atmosphere 時間: 2025-3-22 18:20
Book 2017ics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?Authoritative and cutting-edge, .c-di-GMP Signaling: Methods and Protocols .aims to inspire researchers to try new approaches..作者: immunity 時間: 2025-3-22 22:11 作者: 閑逛 時間: 2025-3-23 01:23
Michal Kedziora,Yang-Wai Chow,Willy Susilodescribe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of . both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.作者: Facilities 時間: 2025-3-23 08:32
Enzymatic Production of c-di-GMP Using a Thermophilic Diguanylate Cyclaseroteins and riboswitches. Importantly, residue replacement in the inhibitory I-site of the thermophilic DGC drastically relieved product inhibition to enable the production of hundreds of milligrams of c-di-GMP using 5–10?mg of this robust biocatalyst.作者: 錯 時間: 2025-3-23 13:30
Gauging and Visualizing c-di-GMP Levels in , Using Fluorescence-Based Biosensorsdescribe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of . both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.作者: 一小塊 時間: 2025-3-23 15:14
High-Performance Liquid Chromatography (HPLC)-Based Detection and Quantitation of Cellular c-di-GMPaphy (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from . samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.作者: deadlock 時間: 2025-3-23 18:34
Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in ,ntrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a .-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.作者: kidney 時間: 2025-3-24 00:15 作者: 得意人 時間: 2025-3-24 04:27
Yufeng Lin,Biplob Ray,Dennis Jarvis,Jia WangG) and 5′-guanosine monophosphate (5′-GMP), as well as cyclic guanosine monophosphate (3′,5′-cGMP) and 3′,3′ c-di-GMP analogues (2′,3′-c-di-GMP and 2′,2′-c-di-GMP) can be simultaneously determined by this method.作者: callous 時間: 2025-3-24 10:20
https://doi.org/10.1007/978-981-10-5421-1ng for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria.作者: 使服水土 時間: 2025-3-24 14:17 作者: 整頓 時間: 2025-3-24 17:34
Applications and Usability of Interactive TV and biofilm phenotypes in . and chemotactic behavior in the alphaproteobacterium .. We expect these light-activated enzymes to be also useful in regulating c-di-GMP-dependent processes occurring at the fast timescale, for spatial control of bacterial populations, as well as for analyzing c-di-GMP-dependent phenomena at the single-cell level.作者: wall-stress 時間: 2025-3-24 20:29 作者: crockery 時間: 2025-3-25 02:50
Lynn Margaret Batten,Mohammed Khalil Amain IRF3 (Interferon regulatory factor 3) for type I interferon production. Here, we describe some experimental procedures such as Isothermal Titration Calorimetry and luciferase reporter assays to study the CDNs binding and activity by STING proteins.作者: 溫和女人 時間: 2025-3-25 05:55 作者: 善辯 時間: 2025-3-25 08:11
Applications and Usability of Interactive TVP-dependent motility, . represents a good model system to assess enzyme activity of diguanylate cyclases and phosphodiesterases using T4P-dependent motility as a readout. Here, we describe the assay, which allows correlating diguanylate cyclase and phosphodiesterase activity with T4P-dependent motility in ..作者: labyrinth 時間: 2025-3-25 12:36 作者: 排他 時間: 2025-3-25 18:27
Detection of Cyclic Dinucleotides by STING IRF3 (Interferon regulatory factor 3) for type I interferon production. Here, we describe some experimental procedures such as Isothermal Titration Calorimetry and luciferase reporter assays to study the CDNs binding and activity by STING proteins.作者: NAUT 時間: 2025-3-25 20:13
Spectrophotometric and Mass Spectroscopic Methods for the Quantification and Kinetic Evaluation of Imetry (UPLC-MS/MS). These methods can be leveraged for a number of experimental applications including the evaluation of enzyme activity for the in vitro synthesis of c-di-GMP, examination of how molecular signals impact these activities, identifying the catalytic properties of hybrid DGC-PDE proteins, and the development of DGC inhibitors.作者: Conduit 時間: 2025-3-26 00:27
Type IV Pili-Dependent Motility as a Tool to Determine the Activity of c-di-GMP Modulating Enzymes iP-dependent motility, . represents a good model system to assess enzyme activity of diguanylate cyclases and phosphodiesterases using T4P-dependent motility as a readout. Here, we describe the assay, which allows correlating diguanylate cyclase and phosphodiesterase activity with T4P-dependent motility in ..作者: 健忘癥 時間: 2025-3-26 05:59 作者: 提煉 時間: 2025-3-26 10:16
Zhuohua Liu,Wei Zhang,Chuankun Wuaphy (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from . samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.作者: 廢墟 時間: 2025-3-26 13:10
Low-Data Complexity Attacks on Camelliantrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a .-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.作者: athlete’s-foot 時間: 2025-3-26 20:19 作者: 滴注 時間: 2025-3-26 23:30 作者: SKIFF 時間: 2025-3-27 03:29 作者: 天然熱噴泉 時間: 2025-3-27 06:50
https://doi.org/10.1007/978-981-13-2907-4ility transition and the switch between the acute and chronic infection status, combined with cell cycle control. The discovery of cyclic di-GMP, though, has been an example par excellence of scientific serendipity. We recapitulate here its years-long discovery process as an activator of the cellulo作者: 漂亮 時間: 2025-3-27 10:55
Anit Kumar Ghosal,Dipanwita Roychowdhurys of c-di-GMP is essential for the biochemical and structural characterization of enzymes and effectors involved in c-di-GMP signaling. Although c-di-GMP can be synthesized using chemical methods, diguanylate cyclases (DGC)-based enzymatic synthesis is the most efficient method of preparing c-di-GMP作者: Polydipsia 時間: 2025-3-27 15:05 作者: 生意行為 時間: 2025-3-27 20:02 作者: myocardium 時間: 2025-3-28 01:23
Yufeng Lin,Biplob Ray,Dennis Jarvis,Jia Wangbacteria and are involved in numerous (patho)physiological settings. Here, we describe a sensitive and specific quantification method for 3′,3′-c-di-GMP by HPLC-coupled tandem mass spectrometry (LC-MS/MS). Additionally, linear 3′,3′-c-di-GMP metabolites, i.e., 5′-phosphoguanylyl-3′,5′-guanosine (pGp作者: 把手 時間: 2025-3-28 03:34
Lynn Margaret Batten,Mohammed Khalil Amainvasion and self-DNA leakage. STING is also a direct receptor for cytosolic cyclic dinucleotides (CDNs), including the microbial secondary messengers c-di-GMP (3′,3′-cyclic di-GMP), 3′,3′cGAMP (3′,3′-cyclic GMP-AMP), and mammalian endogenous 2′,3′cGAMP (2′,3′-cyclic GMP-AMP) synthesized by cGAS (cycl作者: 織布機 時間: 2025-3-28 08:29 作者: 獎牌 時間: 2025-3-28 11:38 作者: hegemony 時間: 2025-3-28 17:40 作者: 衰弱的心 時間: 2025-3-28 20:38
https://doi.org/10.1007/978-981-10-5421-1di-GMP signaling in live bacteria. These experiments are high-throughput, provide information at the single-cell level, and can be performed on cells grown in complex media and/or under anaerobic conditions. Here, we describe flow cytometry methods for three applications: (1) high-throughput screeni作者: Wernickes-area 時間: 2025-3-29 02:42
https://doi.org/10.1007/978-3-030-56574-9e opportunity to interrogate about the roles of specific matrix components in shaping biofilm architecture. Here, we describe two methods optimized for . that profit from morphology and the high level of structural organization of macrocolonies to gain insight into the production and assembly of amy作者: Confound 時間: 2025-3-29 04:38
Cristhian Pachacama,Diego Villamarínere later developed, especially in identifying strains that produce amyloid appendages called curli and overexpressing polysaccharides in the biofilm matrix. The second messenger cyclic diguanylate (c-di-GMP) regulates the production of biofilm matrix polysaccharides, and therefore Congo red stainin作者: zonules 時間: 2025-3-29 09:40 作者: 陰謀小團體 時間: 2025-3-29 11:40 作者: IVORY 時間: 2025-3-29 17:44 作者: 吊胃口 時間: 2025-3-29 23:45
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242717.jpg作者: 離開真充足 時間: 2025-3-30 02:35
c-di-GMP Signaling978-1-4939-7240-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 用不完 時間: 2025-3-30 07:59 作者: capillaries 時間: 2025-3-30 09:34 作者: 財主 時間: 2025-3-30 12:37
