標題: Titlebook: Cytoskeleton Methods and Protocols; Ray H. Gavin Book 20011st edition Humana Press 2001 [打印本頁] 作者: SPARK 時間: 2025-3-21 16:45
書目名稱Cytoskeleton Methods and Protocols影響因子(影響力)
書目名稱Cytoskeleton Methods and Protocols影響因子(影響力)學(xué)科排名
書目名稱Cytoskeleton Methods and Protocols網(wǎng)絡(luò)公開度
書目名稱Cytoskeleton Methods and Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cytoskeleton Methods and Protocols被引頻次
書目名稱Cytoskeleton Methods and Protocols被引頻次學(xué)科排名
書目名稱Cytoskeleton Methods and Protocols年度引用
書目名稱Cytoskeleton Methods and Protocols年度引用學(xué)科排名
書目名稱Cytoskeleton Methods and Protocols讀者反饋
書目名稱Cytoskeleton Methods and Protocols讀者反饋學(xué)科排名
作者: indoctrinate 時間: 2025-3-21 20:28
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242683.jpg作者: 骯臟 時間: 2025-3-22 02:14
An?sthesie bei seltenen Erkrankungen on either side of a known sequence can be problematic with conventional PCR. in which primers extend a complementary chain in a 5′ → 3′ direction. However, if the template DNA is circularized and then used with primers that are oriented with their 3′ ends directed away from each other, amplificatio作者: 凹處 時間: 2025-3-22 04:34 作者: 咆哮 時間: 2025-3-22 09:42
https://doi.org/10.1007/b137887otor that produces directed movement along microtubules. All known dyneins travel to the proximal or minus end of the microtubule. The large dynein complex (M. 1200–2000K) is comprised of several different polypeptides. The smaller subunits mediate the attachment of the dynein to its cargo and regul作者: SPURN 時間: 2025-3-22 16:32
Peter Biro,Dierk Vagts,Thomas Pasch of cytoskeletal filaments, both microtubules and actin filaments have been shown to support vesicle transport. The transport of vesicles is mediated by molecular motors and members of all three super-families of molecular motors-myosin, kinesin and cytoplasmic dynein-have been shown to function as 作者: SPURN 時間: 2025-3-22 18:43
An?sthesie bei seltenen Erkrankungenave evolved, flagellar or ciliary locomotion, and amoeboid locomotion. The latter represents the more general method of cellular locomotion. Amoeboid locomotion in the past has been trivialized. The view, strengthened by bad science fiction movies, was of a cytoplasmic mass, or blob, rolling blindly作者: corpuscle 時間: 2025-3-23 00:01 作者: ANA 時間: 2025-3-23 03:15 作者: 馬籠頭 時間: 2025-3-23 06:04 作者: 濕潤 時間: 2025-3-23 11:46
https://doi.org/10.1007/b137887otility, cell division, and cytoplasmic streaming in plant cells. Several substances are known to influence these processes by altering intracellular actin organization and have been used extensively for cell biological research. Their mechanisms of action are mostly destructive on actin filaments (作者: Schlemms-Canal 時間: 2025-3-23 16:12
An?sthesie bei seltenen Erkrankungen. Each filament system consists of filamentous polymers and their associated proteins. Some of the associated proteins are capable of interconnecting the different filaments systems. The function of the cytoskeleton is manifold; not only is the cytoskeleton responsible for maintaining cell integrity作者: adjacent 時間: 2025-3-23 20:00
https://doi.org/10.1007/b137887are generated by charge interaction of the colloidal gold with the antibody, although the exact nature of this interaction is not fully understood. Colloidal gold can be prepared in sizes ranging from 1 nm to over 30 nm allowing a simultaneous detection of several antigens within the same electron m作者: zonules 時間: 2025-3-23 22:24
An?sthesie bei seltenen Erkrankungentems. Ultimately, the function of a component has to be studied within intact cell systems. A problem, which one has to be overcome, is the delivery of the antibody into the cells. The delivery of antibodies can be a particular problem either, if large numbers of cells will be analyzed or if one wan作者: 戰(zhàn)勝 時間: 2025-3-24 03:42
Peter Biro,Manfred Abel,Thomas Paschcrotubules. Microfilaments are composed of assembled globular actin monomers that form a filamentous system involved in the maintenance of cell shape and polarity. Intermediate filaments are made of fibrous proteins including vimentin, cytokeratin, nuclear lamins, and neurofilament proteins that ass作者: 音樂戲劇 時間: 2025-3-24 09:10
Peter Biro,Manfred Abel,Thomas Paschvisible fluorescence without the requirement of cofactors or other enzymes. In vivo, the photoprotein aequorin binds dimers of GFP allowing the blue light emitted by aequorin to be converted to green light (.). This visible fluorescence emitted by GFP is made possible by an internal .-hydroxybenzyli作者: 不法行為 時間: 2025-3-24 13:17 作者: DECRY 時間: 2025-3-24 15:41 作者: 拱墻 時間: 2025-3-24 21:50 作者: 繞著哥哥問 時間: 2025-3-25 01:35
Peter Biro,Dierk Vagts,Thomas Paschby molecular motors and members of all three super-families of molecular motors-myosin, kinesin and cytoplasmic dynein-have been shown to function as vesicle motors (.–.). The specific types of vesicles transported by some of the molecular motors have been identified (.–.).作者: 悲觀 時間: 2025-3-25 05:44
An?sthesie bei seltenen Erkrankungen function of the cytoskeleton is of central importance for the understanding of cell motility, and conversely, investigations of cell motility may shed light on the function of cytoskeletal components.作者: 并入 時間: 2025-3-25 07:52 作者: bacteria 時間: 2025-3-25 12:50
An?sthesie bei seltenen Erkrankungenthe different filaments systems. The function of the cytoskeleton is manifold; not only is the cytoskeleton responsible for maintaining cell integrity and cell shape; it is also involved in cell locomotion and intracellular transport.作者: 凌辱 時間: 2025-3-25 15:52 作者: interrogate 時間: 2025-3-25 21:13 作者: PLAYS 時間: 2025-3-26 03:08
https://doi.org/10.1007/b137887 will present here our methods of preparing colloidal gold-conjugated primary antibodies. In combination with a modified technique of negative staining these primary antibodies can then be employed to identify subunits within protein complexes.作者: plasma 時間: 2025-3-26 05:51
Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy will present here our methods of preparing colloidal gold-conjugated primary antibodies. In combination with a modified technique of negative staining these primary antibodies can then be employed to identify subunits within protein complexes.作者: 摻和 時間: 2025-3-26 11:38 作者: 藕床生厭倦 時間: 2025-3-26 13:02 作者: 山崩 時間: 2025-3-26 20:06
Evaluation of Individual-Cell Motility function of the cytoskeleton is of central importance for the understanding of cell motility, and conversely, investigations of cell motility may shed light on the function of cytoskeletal components.作者: incubus 時間: 2025-3-26 21:36
Evaluation of Cell Morphologyation of cytoskeletal components often are so pronounced that quantification may seem unnecessary. However, quantitative determination of cellular morphology greatly expands the possibility of characterizing the role and regulation of macromolecules involved, e.g., in cytoskeletal functions and cellular differentiation.作者: Minikin 時間: 2025-3-27 03:02
Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteinsthe different filaments systems. The function of the cytoskeleton is manifold; not only is the cytoskeleton responsible for maintaining cell integrity and cell shape; it is also involved in cell locomotion and intracellular transport.作者: gnarled 時間: 2025-3-27 07:12 作者: 手榴彈 時間: 2025-3-27 11:39
Peter Biro,Dierk Vagts,Thomas Paschors” where correlations between cellular behaviour, cell behaviour and molecular signal transduction events can be studied experimentally because many ciliates are easy to grow and handle in the laboratory.作者: Expressly 時間: 2025-3-27 13:43 作者: 半導(dǎo)體 時間: 2025-3-27 21:44 作者: SLING 時間: 2025-3-27 23:04 作者: EXCEL 時間: 2025-3-28 02:47
Peter Biro,Manfred Abel,Thomas Pasch, rigid polymers which govern the location of membrane-bounded organelles and other cellular components. While each filament system has specific cellular functions, an emerging view of the cytoskeleton is that actin, intermediate filaments and microtubule networks function in concert to provide the cell with stability, polarity and organization.作者: 燈絲 時間: 2025-3-28 09:49 作者: Handedness 時間: 2025-3-28 12:33
Microsequencing of Myosins for PCR Primer Designbe obtained from myosin proteins whose biochemical behavior was known, then the gap between function and sequence could be bridged. We describe here a method that enabled us to acquire peptide sequences of semi-purified myosins (.).作者: Oratory 時間: 2025-3-28 18:28 作者: Accessible 時間: 2025-3-28 19:15 作者: 燈泡 時間: 2025-3-28 23:16 作者: Latency 時間: 2025-3-29 03:45
Microsequencing of Myosins for PCR Primer Design peptide sequences by standard protocols. For this reason, instead of identifying myosins first according to their biochemical activity and then obtaining their sequences, PCR and other DNA-based techniques exploiting the highly conserved sequences in the amino end, the “head” domain, have been used作者: COLON 時間: 2025-3-29 10:44 作者: Defense 時間: 2025-3-29 13:26
Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function of cytoskeletal filaments, both microtubules and actin filaments have been shown to support vesicle transport. The transport of vesicles is mediated by molecular motors and members of all three super-families of molecular motors-myosin, kinesin and cytoplasmic dynein-have been shown to function as 作者: 蛛絲 時間: 2025-3-29 16:05
Computer-Assisted Systems for the Analysis of Amoeboid Cell Motilityave evolved, flagellar or ciliary locomotion, and amoeboid locomotion. The latter represents the more general method of cellular locomotion. Amoeboid locomotion in the past has been trivialized. The view, strengthened by bad science fiction movies, was of a cytoplasmic mass, or blob, rolling blindly作者: 相容 時間: 2025-3-29 20:18
Evaluation of Individual-Cell Motilityon of a large number of molecular components including all main structures of the cytoskeleton (.,.). Thus, a detailed knowledge of the regulation and function of the cytoskeleton is of central importance for the understanding of cell motility, and conversely, investigations of cell motility may she作者: 打折 時間: 2025-3-30 03:13 作者: 博識 時間: 2025-3-30 04:52 作者: instulate 時間: 2025-3-30 08:24
Jasplakinolideotility, cell division, and cytoplasmic streaming in plant cells. Several substances are known to influence these processes by altering intracellular actin organization and have been used extensively for cell biological research. Their mechanisms of action are mostly destructive on actin filaments (作者: 沖突 時間: 2025-3-30 14:25
Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteins. Each filament system consists of filamentous polymers and their associated proteins. Some of the associated proteins are capable of interconnecting the different filaments systems. The function of the cytoskeleton is manifold; not only is the cytoskeleton responsible for maintaining cell integrity作者: growth-factor 時間: 2025-3-30 19:02
Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopyare generated by charge interaction of the colloidal gold with the antibody, although the exact nature of this interaction is not fully understood. Colloidal gold can be prepared in sizes ranging from 1 nm to over 30 nm allowing a simultaneous detection of several antigens within the same electron m作者: intuition 時間: 2025-3-30 21:24 作者: esoteric 時間: 2025-3-31 03:20
Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Proteincrotubules. Microfilaments are composed of assembled globular actin monomers that form a filamentous system involved in the maintenance of cell shape and polarity. Intermediate filaments are made of fibrous proteins including vimentin, cytokeratin, nuclear lamins, and neurofilament proteins that ass作者: 通便 時間: 2025-3-31 07:18 作者: Psa617 時間: 2025-3-31 09:12
Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamicsin vivo, the IF cytoskeleton is composed of heteropolymers of type I (acidic) and type II (neutral-basic) keratins, whose expression is carefully controlled in a cell type- and differentiation-specific manner (.–.). In addition, many epithelial cells in culture also induce the synthesis of the IF pr作者: chassis 時間: 2025-3-31 14:13 作者: 騎師 時間: 2025-3-31 20:07 作者: outer-ear 時間: 2025-3-31 21:53
An?sthesie bei seltenen Erkrankungen,.), and making sharp turns through lateral pseudopod formation (.–.). Animal cells spanning the eukaryotic world, from . amoeba to human white blood cells, also exhibit a one minute behavior cycle that, in some cases, has a z-axis component (.,.,.,.).
