標(biāo)題: Titlebook: Cytoskeleton Methods and Protocols; Ray H. Gavin Book 2016Latest edition Springer Science+Business Media New York 2016 Cell and Organelle [打印本頁(yè)] 作者: 麻煩 時(shí)間: 2025-3-21 17:19
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols影響因子(影響力)
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols被引頻次
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols被引頻次學(xué)科排名
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols年度引用
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols年度引用學(xué)科排名
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols讀者反饋
書(shū)目名稱(chēng)Cytoskeleton Methods and Protocols讀者反饋學(xué)科排名
作者: humectant 時(shí)間: 2025-3-21 22:54
Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeastgeneration and muscle myopathies, and overall cellular aging are marked by declining mitochondrial function and subsequent loss of multiple other cellular functions. For these reasons, optimized protocols are needed for visualization and quantification of mitochondria and their function and fitness.作者: spondylosis 時(shí)間: 2025-3-22 03:55
Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cellsshape, and rigid cell wall created obstacles to explore the cell biology of this model eukaryote. It is now possible to acquire and analyze high-resolution and super-resolution multidimensional images of the yeast cell. As a result, imaging of yeast has emerged as an important tool in eukaryotic cel作者: strain 時(shí)間: 2025-3-22 07:11
Imaging of the Cytoskeleton Using Live and Fixed , Tissue Culture Cellsm. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe . S2 cell culture and preparation for live-cell and fixed-cell flu作者: 匯總 時(shí)間: 2025-3-22 11:44
Imaging Cytoskeleton Components by Electron Microscopyical properties of a cell. Fibrillar polymers—actin filaments, microtubules, and intermediate filaments—are major constituents of the cytoskeleton, which constantly change their organization during cellular activities. The actin cytoskeleton is especially polymorphic, as actin filaments can form mul作者: GENUS 時(shí)間: 2025-3-22 14:17
Purification and Localization of Intraflagellar Transport Particles and PolypeptidesThis includes the primary cilia of most human cells that are in the G. phase of the cell cycle. The model system for the study of IFT is the flagella of the biflagellate green alga .. It is in this organism that IFT was first discovered, and genetic data from a . mutant first linked the process of I作者: GENUS 時(shí)間: 2025-3-22 20:59
Fluorescence Imaging of the Cytoskeleton in Plant Rootsowever, to make full use of these live cell reporters it is necessary to implement simple methods to maintain plant specimens in optimal growing conditions during imaging. To image the cytoskeleton in living Arabidopsis roots, we rely on a system involving coverslips coated with nutrient supplemente作者: Favorable 時(shí)間: 2025-3-22 22:12
Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Minalyze them. This chapter provides specific methods that can be used to analyze microtubule organization and dynamic properties in plant systems and summarizes the advantages and limitations for each technique. We outline basic methods for preparing samples for immunofluorescence labeling, including作者: 放大 時(shí)間: 2025-3-23 04:54
Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation ofry to characterize effects of the protein on actin filament dynamics in vitro. This chapter describes basic microscopic methods to visualize fluorescently labeled actin filaments using commonly available fluorescence microscope settings. Direct microscopic observation of actin filaments provides str作者: Disk199 時(shí)間: 2025-3-23 07:28
An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Celle developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the . Type IV pili in the same order of magnitude than the forces generated by live bacteria. We s作者: 勉強(qiáng) 時(shí)間: 2025-3-23 13:43
Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes Myosin driven contractility and actin cytoskeleton membrane interactions form the basis of fundamental cellular processes such as cytokinesis, cell migration, and cortical flows. How the interplay between the actin cytoskeleton, the membrane, and actin binding proteins drives these processes is far作者: minaret 時(shí)間: 2025-3-23 15:09 作者: 自作多情 時(shí)間: 2025-3-23 21:51
Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondram on morphogenesis, motility, organelle movement, or when apoptosis has to be induced. These substances can be divided into two major subclasses: F-actin-stabilizing and -polymerizing substances like jasplakinolide and chondramides and F-actin-severing compounds like chytochalasins and latrunculins. 作者: 四指套 時(shí)間: 2025-3-23 23:36
Quantitative Motion Analysis in Two and Three Dimensions analysis of dynamic cell shape changes that occur through extension and retraction of force generating structures such as pseudopodia and lamellipodia. Quantitative analysis of these structures is an underutilized tool in the field of cell migration. Our intent, therefore, is to present methods tha作者: Thyroxine 時(shí)間: 2025-3-24 05:21
Measurement of Cell Motility Using Microgrooved Substratesl migration is important not only for fundamental cell biology research, but also to understand tissue morphogenesis and wound healing. In this chapter, we describe a method developed in our laboratory to measure cell migration in a uniaxial direction. In this approach, linear microgrooves, fabricat作者: 符合國(guó)情 時(shí)間: 2025-3-24 07:17
The Study of Cell Motility by Cell Traction Force Microscopy (CTFM)an be measured by monitoring cell traction forces, which are generated by individual cells and transmitted to the substrate below the migrant cells. This method, termed cell traction force microscopy (CTFM), has the advantage of directly measuring the “cause” (i.e., cell traction forces, CTFs) of ce作者: Affluence 時(shí)間: 2025-3-24 14:41 作者: 伸展 時(shí)間: 2025-3-24 17:00 作者: 濃縮 時(shí)間: 2025-3-24 20:46 作者: Sigmoidoscopy 時(shí)間: 2025-3-25 00:40 作者: 詳細(xì)目錄 時(shí)間: 2025-3-25 04:31 作者: chance 時(shí)間: 2025-3-25 08:40
Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution tns and gives insight into their endogenous localization. Moreover, we outline an extracellular matrix (ECM) degradation assay as an application of the general protocol. By seeding cells onto a fluorescently labeled gelatin matrix, degradation can be quantified by means of a matrix degradation index.作者: flutter 時(shí)間: 2025-3-25 13:39 作者: 溫順 時(shí)間: 2025-3-25 15:48 作者: SPECT 時(shí)間: 2025-3-25 22:50
Book 2016Latest editionnformation aimed at ensuring success with implementation of the protocols..Authoritative and thorough, .Cytoskeleton Methods and Protocols, Third Edition. helps researchers expand their understanding of cytoskeleton structure and function..作者: Modicum 時(shí)間: 2025-3-26 01:45 作者: 偽造者 時(shí)間: 2025-3-26 06:08 作者: 殖民地 時(shí)間: 2025-3-26 11:53
An?sthesie bei seltenen Erkrankungen fixed plant material via indirect immunofluorescence. For these methods we typically use non-embedded vibratome-sectioned and whole mount permeabilized root tissue. The clearly defined developmental regions of the root provide us with an elegant system to further understand the cytoskeletal basis o作者: Exhilarate 時(shí)間: 2025-3-26 14:41 作者: 拾落穗 時(shí)間: 2025-3-26 19:46
https://doi.org/10.1007/978-3-662-11140-6ns and gives insight into their endogenous localization. Moreover, we outline an extracellular matrix (ECM) degradation assay as an application of the general protocol. By seeding cells onto a fluorescently labeled gelatin matrix, degradation can be quantified by means of a matrix degradation index.作者: Explosive 時(shí)間: 2025-3-26 21:36 作者: OCTO 時(shí)間: 2025-3-27 03:34
An?sthesie bei seltenen Erkrankungen model for high-resolution analysis of cancer cells from cell lines and human cancer tissue in a 3D matrix. Use of this model led to the discovery of the coalescence of cancer cell aggregates and unique cell behaviors not seen in normal cells or normal tissue. Graphic illustrations to visually displ作者: misshapen 時(shí)間: 2025-3-27 07:28
Frans H. Van Eemeren,Peter Houtlosser cells of the skin represent the front line of defense against invasive pathogens such as . but under certain circumstances, especially when the host’s immune system is compromised, the skin barrier is breached. The mechanisms by which the fungal pathogen penetrates the skin and invade the deeper la作者: 朝圣者 時(shí)間: 2025-3-27 11:56
An?sthesie bei seltenen Erkrankungengeneration and muscle myopathies, and overall cellular aging are marked by declining mitochondrial function and subsequent loss of multiple other cellular functions. For these reasons, optimized protocols are needed for visualization and quantification of mitochondria and their function and fitness.作者: 繁忙 時(shí)間: 2025-3-27 17:10 作者: 業(yè)余愛(ài)好者 時(shí)間: 2025-3-27 21:41 作者: 低三下四之人 時(shí)間: 2025-3-28 01:13 作者: 并排上下 時(shí)間: 2025-3-28 05:44 作者: mucous-membrane 時(shí)間: 2025-3-28 06:29 作者: Ischemic-Stroke 時(shí)間: 2025-3-28 14:11
An?sthesie bei seltenen Erkrankungennalyze them. This chapter provides specific methods that can be used to analyze microtubule organization and dynamic properties in plant systems and summarizes the advantages and limitations for each technique. We outline basic methods for preparing samples for immunofluorescence labeling, including作者: 寬度 時(shí)間: 2025-3-28 17:11 作者: CYN 時(shí)間: 2025-3-28 22:20
https://doi.org/10.1007/978-3-662-11140-6e developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the . Type IV pili in the same order of magnitude than the forces generated by live bacteria. We s作者: 單挑 時(shí)間: 2025-3-29 02:01 作者: 不在灌木叢中 時(shí)間: 2025-3-29 04:07
https://doi.org/10.1007/978-3-662-11140-6oteins. Traditional approaches make use of protein overexpression or siRNA. However to study or modulate resident endogenous proteins, complementary methods are required. Since the discovery of nanobodies in 1993, they have proven to represent interesting tools in a variety of applications due to th作者: garrulous 時(shí)間: 2025-3-29 08:43
An?sthesie bei seltenen Erkrankungen on morphogenesis, motility, organelle movement, or when apoptosis has to be induced. These substances can be divided into two major subclasses: F-actin-stabilizing and -polymerizing substances like jasplakinolide and chondramides and F-actin-severing compounds like chytochalasins and latrunculins. 作者: placebo-effect 時(shí)間: 2025-3-29 13:30
An?sthesie bei seltenen Erkrankungen analysis of dynamic cell shape changes that occur through extension and retraction of force generating structures such as pseudopodia and lamellipodia. Quantitative analysis of these structures is an underutilized tool in the field of cell migration. Our intent, therefore, is to present methods tha作者: opprobrious 時(shí)間: 2025-3-29 19:06 作者: 細(xì)絲 時(shí)間: 2025-3-29 21:50 作者: exophthalmos 時(shí)間: 2025-3-30 03:09 作者: engagement 時(shí)間: 2025-3-30 07:06 作者: 缺陷 時(shí)間: 2025-3-30 08:38 作者: 侵蝕 時(shí)間: 2025-3-30 13:33 作者: 漫不經(jīng)心 時(shí)間: 2025-3-30 18:09
978-1-4939-4975-5Springer Science+Business Media New York 2016作者: Muffle 時(shí)間: 2025-3-31 00:04
Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast In budding yeast, mitochondria are intimately associated with the actin cytoskeleton and utilize actin for their movement and inheritance. This chapter describes optimal approaches for labeling mitochondria and the actin cytoskeleton in living budding yeast cells, for imaging the labeled cells, and for analyzing the resulting images.
