作者: 拋射物 時(shí)間: 2025-3-21 23:35 作者: SUGAR 時(shí)間: 2025-3-22 01:20 作者: 低能兒 時(shí)間: 2025-3-22 08:09 作者: 助記 時(shí)間: 2025-3-22 08:58 作者: Libido 時(shí)間: 2025-3-22 13:19 作者: Libido 時(shí)間: 2025-3-22 19:59 作者: Deject 時(shí)間: 2025-3-22 21:23 作者: 逢迎春日 時(shí)間: 2025-3-23 02:18 作者: buoyant 時(shí)間: 2025-3-23 06:25 作者: 決定性 時(shí)間: 2025-3-23 12:27 作者: 溺愛(ài) 時(shí)間: 2025-3-23 14:47
Evidence for Anxiety Management Trainingcomposed of microtubules (MTs) and MT-associated proteins that help align and segregate the chromosomes. The localization and characterization of many spindle proteins have been greatly aided by using GFP-tagged proteins in vivo, but these tools typically do not allow for understanding how their act作者: 高調(diào) 時(shí)間: 2025-3-23 21:52 作者: 失敗主義者 時(shí)間: 2025-3-23 23:26
Assessment of Depression and Mood Disorders bacterial cells with high temporal resolution. This chapter describes computerized analysis methods to quantitatively characterize the dynamics and morphological properties of MreB assemblies. These include how to (1) segment bacterial cells, (2) perform single-particle tracking (SPT) of MreB filam作者: 偏狂癥 時(shí)間: 2025-3-24 05:02 作者: Enzyme 時(shí)間: 2025-3-24 06:56
Foundations of Developmental Psychopathologyods for measuring nucleation activity at centrosomes and at mitotic chromatin in cell lines, to study interphase and mitotic microtubule organization, and for measuring non-centrosomal?nucleation in cultured primary neurons, to study microtubule organization in the absence of a microtubule organizin作者: 心胸開(kāi)闊 時(shí)間: 2025-3-24 13:39
Building Resilience Through Preventionto a large extent comprised of Golgi-derived MTs (GDMTs), which asymmetrically extend toward the cell front. We have recently found that GDMT asymmetry is based on a nonrandom positioning of spatially restricted nucleation hotspots, where MTs form in a cooperative manner. Here, we summarize methods 作者: KIN 時(shí)間: 2025-3-24 18:47 作者: 沙草紙 時(shí)間: 2025-3-24 21:25
Wendy K. Silverman,William M. Kurtineslar cytoskeleton dynamics at much faster timescales than by genetic modification. For example, in mammalian cells, microtubules (MTs) grow tens of micrometers per minute and many intracellular MT functions are mediated by a complex of +TIP proteins that dynamically associate with growing MT plus end作者: 煩人 時(shí)間: 2025-3-25 02:35
Wendy K. Silverman,William M. Kurtinesrotubular cytoskeleton reorganizes to assemble a mitotic spindle necessary for chromosome segregation. Several methods, such as controlled exposure to cold, high pressure, high calcium concentration, or microtubule depolymerizing drugs, have been widely used to evaluate the dynamic properties of spe作者: 極深 時(shí)間: 2025-3-25 04:39
Wendy K. Silverman,William M. Kurtinesregate chromosomes. Several model systems have been widely used to dissect the molecular and structural mechanisms behind mitotic spindle assembly and function. These include budding and fission yeasts, which are ideal for genetic and molecular approaches, but show limitations in high-resolution liv作者: 證明無(wú)罪 時(shí)間: 2025-3-25 08:22
Clinical Child Psychology Libraryules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution 作者: 小溪 時(shí)間: 2025-3-25 13:24
Panic Spectrum Disorders and Substance Usee highly regulated assembly and constriction of an actomyosin contractile ring, whose function is to pinch the mother cell in two. Research on the contractile ring has particularly focused on the signaling mechanisms that dictate when and where the ring is formed. In vivo studies of ring constrictio作者: 山頂可休息 時(shí)間: 2025-3-25 17:27 作者: Panacea 時(shí)間: 2025-3-25 22:44 作者: Fracture 時(shí)間: 2025-3-26 00:23
Optogenetic Control of Microtubule Dynamics,gh-resolution live-cell microscopy in combination with π-EB1 photodissociation. However, these techniques are broadly applicable to other LOV2-based and likely other blue light-sensitive optogenetics. In addition to being a tool to investigate +TIP functions acutely and with subcellular resolution, 作者: 一罵死割除 時(shí)間: 2025-3-26 08:00
Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation ofsure spindle microtubule dynamics using photoactivation of fluorescently tagged tubulin in living cells. This methodology allows the quantitative discrimination of the turnover of specific microtubule populations (e.g., kinetochore vs. non-kinetochore microtubules), as well as determination of micro作者: Urea508 時(shí)間: 2025-3-26 11:54 作者: 率直 時(shí)間: 2025-3-26 14:15
Knocking Out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifical enzymes in parallel to obtain a significant change in a given tubulin modification. Here we describe a method to generate primary cells with combinatorial knockout genotypes using conditional knockout mice. The conditional alleles are converted into knockout in the cultured primary cells by transd作者: ALB 時(shí)間: 2025-3-26 20:41 作者: elastic 時(shí)間: 2025-3-26 22:09 作者: 演繹 時(shí)間: 2025-3-27 02:57
Wendy K. Silverman,William M. Kurtinesgh-resolution live-cell microscopy in combination with π-EB1 photodissociation. However, these techniques are broadly applicable to other LOV2-based and likely other blue light-sensitive optogenetics. In addition to being a tool to investigate +TIP functions acutely and with subcellular resolution, 作者: Inelasticity 時(shí)間: 2025-3-27 06:26 作者: ethereal 時(shí)間: 2025-3-27 11:35 作者: 萬(wàn)花筒 時(shí)間: 2025-3-27 17:27 作者: Formidable 時(shí)間: 2025-3-27 18:35
Book 2020e an invaluable resource for present and future generations of cytoskeleton researchers. .The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via l作者: 無(wú)聊點(diǎn)好 時(shí)間: 2025-3-27 23:07
https://doi.org/10.1007/978-1-4899-3567-0k-field microscopy. The ability to image microtubules label-free allows the investigation of the dynamic properties of non-abundant tubulin species where fluorescent labeling is not feasible, free from the confounding effects arising from the addition of fluorescent labels.作者: FECK 時(shí)間: 2025-3-28 05:13 作者: 重力 時(shí)間: 2025-3-28 06:38
In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy,k-field microscopy. The ability to image microtubules label-free allows the investigation of the dynamic properties of non-abundant tubulin species where fluorescent labeling is not feasible, free from the confounding effects arising from the addition of fluorescent labels.作者: 率直 時(shí)間: 2025-3-28 13:37 作者: BILIO 時(shí)間: 2025-3-28 15:57 作者: 地名表 時(shí)間: 2025-3-28 20:08 作者: 損壞 時(shí)間: 2025-3-29 02:05
The Developmental Psychopathology of Anxietyellular function and integrity. However, their small size poses a challenge to study them. Here, we describe protocols that allow the identification and assessment of true centrioles and that provide straightforward strategies to study the role of new candidate proteins in centriole duplication and elongation.作者: 諂媚于人 時(shí)間: 2025-3-29 05:39
Clinical Child Psychology Librarygregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.作者: 懶洋洋 時(shí)間: 2025-3-29 08:05 作者: Uncultured 時(shí)間: 2025-3-29 13:15
Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-Liorphological properties of MreB assemblies. These include how to (1) segment bacterial cells, (2) perform single-particle tracking (SPT) of MreB filamentous structures, (3) classify their dynamic modes using mean squared displacement (MSD) analysis, and (4) measure their dimensions and orientation.作者: beta-carotene 時(shí)間: 2025-3-29 18:15
Studying Centriole Duplication and Elongation in Human Cells,ellular function and integrity. However, their small size poses a challenge to study them. Here, we describe protocols that allow the identification and assessment of true centrioles and that provide straightforward strategies to study the role of new candidate proteins in centriole duplication and elongation.作者: AXIS 時(shí)間: 2025-3-29 20:03
Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocygregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.作者: ANTIC 時(shí)間: 2025-3-30 02:00
Kwan Woo Choi,Yong-Ku Kim,Hong Jin Jeon, and the isolation of co-purifying tubulin-associated proteins (TAPs) in mammalian cells. This approach is currently used in our laboratory to study tubulin function and to identify and characterize TAPs.作者: extinct 時(shí)間: 2025-3-30 07:23
Overview of Anxiety Management Trainingay enabling the characterization of Tau interaction with dynamic microtubules at the single-molecule level. We describe protein sample preparation in flow cells, single-molecule acquisitions by TIRF microscopy, and quantitative analysis of Tau oligomerization states and dwell time on microtubules.作者: 來(lái)這真柔軟 時(shí)間: 2025-3-30 10:15
Conduct Problems and Anxiety in Children,ortical MreB assemblies in vivo. This chapter describes the methods for visualizing fluorescently tagged MreB proteins in live . cells. We detail how to (1) grow . strains for reproducible TIRF observations, (2) immobilize cells on agarose pads and (3) in CellASIC. microfluidic plates, and (4) acquire TIRF images and time lapses.作者: 節(jié)約 時(shí)間: 2025-3-30 15:01
Foundations of Developmental Psychopathologyg center. While a number of different approaches and variations thereof have been reported in the literature, here we aim to keep the methodology as simple as possible and thus accessible to most research laboratories.作者: 小歌劇 時(shí)間: 2025-3-30 19:46 作者: 蝕刻術(shù) 時(shí)間: 2025-3-31 00:42 作者: 富饒 時(shí)間: 2025-3-31 01:54
Overview of Anxiety Management Training made to interact with actin filaments through a tip-tracking complex consisting of microtubule end-binding proteins and an actin-microtubule cytolinker. In addition to the protocols themselves, we discuss the optimization steps required in order to build these more complex in vitro model systems of cytoskeletal interactions.作者: 土產(chǎn) 時(shí)間: 2025-3-31 06:30
Evidence for Anxiety Management Traininging useful tools for the quantitative analysis of protein activity and protein-protein interactions. Here, we describe solution-based F?rster resonance energy transfer (FRET) and fluorescence assays that can be used to quantify protein-protein interactions and to characterize protein conformations of MT-associated proteins involved in mitosis.作者: Phonophobia 時(shí)間: 2025-3-31 11:48 作者: Collision 時(shí)間: 2025-3-31 17:03 作者: 散布 時(shí)間: 2025-3-31 20:49
