標(biāo)題: Titlebook: Cytokine Bioassays; Methods and Protocol Ivana Vancurova Book 2014 Springer Science+Business Media New York 2014 Cytokines.RT-PCR.analysis [打印本頁] 作者: retort 時(shí)間: 2025-3-21 16:26
書目名稱Cytokine Bioassays影響因子(影響力)
書目名稱Cytokine Bioassays影響因子(影響力)學(xué)科排名
書目名稱Cytokine Bioassays網(wǎng)絡(luò)公開度
書目名稱Cytokine Bioassays網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cytokine Bioassays被引頻次
書目名稱Cytokine Bioassays被引頻次學(xué)科排名
書目名稱Cytokine Bioassays年度引用
書目名稱Cytokine Bioassays年度引用學(xué)科排名
書目名稱Cytokine Bioassays讀者反饋
書目名稱Cytokine Bioassays讀者反饋學(xué)科排名
作者: HILAR 時(shí)間: 2025-3-21 20:18
Assessment of Cytokine-Modulated Proteasome Activityy and its relationship to cytokine action and inflammation are clear, the assessment and interpretation is not always straightforward. Therefore, we also discuss the pitfalls of the standard fluorogenic assay, particularly in the interpretation of results obtained, and the advantages of the newer, ProCISE assay.作者: 自負(fù)的人 時(shí)間: 2025-3-22 03:02
Enhanced ELISA Based on Carboxymethylated Dextran Coatingsue step corresponding to the covalent grafting of the capture antibody on a carboxymethylated dextran (CMD) layer, a single step completed in 15 min. Taking advantage of the CMD low-fouling properties, blocking agent-free buffer solutions can be used as diluent in the improved approach.作者: infelicitous 時(shí)間: 2025-3-22 04:50
Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylatio is cell surface biotinylation. Biotinylated surface proteins can be rapidly purified through the strong interaction between biotin and streptavidin. Here, we describe the procedure for surface biotinylation and purification of biotinylated cytokine receptors for further downstream analysis.作者: 委托 時(shí)間: 2025-3-22 10:38 作者: Cabg318 時(shí)間: 2025-3-22 16:11 作者: Cabg318 時(shí)間: 2025-3-22 20:27 作者: motor-unit 時(shí)間: 2025-3-23 00:46 作者: 雪白 時(shí)間: 2025-3-23 04:40 作者: DNR215 時(shí)間: 2025-3-23 07:31 作者: 不真 時(shí)間: 2025-3-23 12:18
https://doi.org/10.1007/978-1-349-21483-9ents that lead to switching the immunoglobulin isotopes from IgM to IgE. This chapter describes the methodology of class switch recombination assay for assessment of the effect of the environmental pollutants in toxicological laboratory diagnostics.作者: fleeting 時(shí)間: 2025-3-23 15:08 作者: 進(jìn)取心 時(shí)間: 2025-3-23 18:48 作者: 大約冬季 時(shí)間: 2025-3-24 02:09
Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytacrophages, which can easily be applied to other phagocytes. Each method is based on the extent of engulfment of FITC-labeled latex minibeads by macrophages under different conditions. Phagocytic activity can be assessed either by counting individual cells using a fluorescence microscope or measuring fluorescence intensity using a flow cytometer.作者: Enrage 時(shí)間: 2025-3-24 03:24 作者: acheon 時(shí)間: 2025-3-24 07:02 作者: 柔美流暢 時(shí)間: 2025-3-24 11:53 作者: 強(qiáng)制令 時(shí)間: 2025-3-24 17:58
Cytokine-Induced Neutrophil Chemotaxis Assayasis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known. This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.作者: 廣告 時(shí)間: 2025-3-24 23:05 作者: Paradox 時(shí)間: 2025-3-25 02:39 作者: 顧客 時(shí)間: 2025-3-25 03:49 作者: conifer 時(shí)間: 2025-3-25 08:26
Book 2014ng inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenge作者: 比喻好 時(shí)間: 2025-3-25 15:09 作者: neutral-posture 時(shí)間: 2025-3-25 18:15
A. Angerhofer,J. U. von Schütz,H. C. Wolfcells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.作者: 形容詞詞尾 時(shí)間: 2025-3-25 20:22
Andrew J. Poggio,Edmund K. Millerirtue of the exquisite sensitivity of the ELISPOT assay, frequency analysis of rare cell populations (e.g., antigen-specific responses), which was not possible before, is now relatively easy. However, development of a method sensitive enough to pinpoint elusive immune markers at the single-cell leve作者: 易于 時(shí)間: 2025-3-26 03:55
A. Angerhofer,J. U. von Schütz,H. C. Wolfcells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.作者: SLUMP 時(shí)間: 2025-3-26 06:41 作者: 啪心兒跳動(dòng) 時(shí)間: 2025-3-26 09:01
Jürgen H?her,Christoph Offerhaus. an antibody adsorbed to the wells of a microplate. Plate preparation for standard assay involves a passive adsorption of capture antibodies followed by the incubation of blocking agents. Here, we describe a new strategy that replaces these two time-consuming adsorption steps (up to 15 h) by a uniq作者: AROMA 時(shí)間: 2025-3-26 13:17 作者: 商品 時(shí)間: 2025-3-26 20:50
Milos Dordevic,Michael T. Hirschmann MDility of measuring multiple variables is important for discovery of biomarkers of pathogenesis or protection in complex diseases as well as measurement of antigen-specific cellular responses. Measurements can be made in biological specimens, such as plasma or serum, cell culture supernatants, and ot作者: probate 時(shí)間: 2025-3-26 23:21 作者: 冥界三河 時(shí)間: 2025-3-27 02:20 作者: 深淵 時(shí)間: 2025-3-27 07:02 作者: GIDDY 時(shí)間: 2025-3-27 10:14
Anterior knee pain and patellar instabilitycroenvironment have been shown to enhance intravasation and metastatic spread of breast carcinomas. We describe an in vitro motility assay that combines time-lapse wide-field microscopy and micro-patterned linear adhesive substrates to reconstitute the in vivo behavior between macrophages, tumor cel作者: 左右連貫 時(shí)間: 2025-3-27 15:14 作者: 貧困 時(shí)間: 2025-3-27 18:21
Implikationen für die Marketingforschungparticles, and clear cell debris. Phagocytes and their ability to phagocytose are an important part of the innate immune system and are critical for homeostasis of the host. Impairment in phagocytosis has been associated with numerous diseases and disorders. Different cytokines have been shown to af作者: 替代品 時(shí)間: 2025-3-27 23:36
https://doi.org/10.1057/9780230374164y which takes into account the effect of ATP on proteasome activity. This method is described in both its macro and high throughput micro-assay forms. The second is the .teasome .onstitutive .mmuno-.ubunit (active site) .LISA or ProCISE method. ProCISE is a modification of active site directed probe作者: 下垂 時(shí)間: 2025-3-28 03:52 作者: Nefarious 時(shí)間: 2025-3-28 07:45 作者: lobster 時(shí)間: 2025-3-28 10:34
https://doi.org/10.1007/978-94-009-7630-6esponses by binding to cytokine receptors on the cell plasma membrane. Thus, knowledge of the expression level of particular cytokine receptors on cell surface is crucial for understanding the cytokine function and regulation. One of the techniques to explore the membrane embedded cytokine receptors作者: Narrative 時(shí)間: 2025-3-28 16:12
https://doi.org/10.1007/978-94-009-7630-6se, cell proliferation, migration, and survival. IL-8 expression is increased not only in inflammatory disorders, but also in many types of cancer, including prostate cancer. IL-8 acts as a ligand for the C-X-C chemokine receptor 2 (CXCR2) protein present on the cell plasma membrane. Binding of the 作者: 暫時(shí)過來 時(shí)間: 2025-3-28 19:56 作者: interference 時(shí)間: 2025-3-29 01:45
https://doi.org/10.1007/978-1-4939-0928-5Cytokines; RT-PCR; analysis of intracellular cytokines by flow cytometry; biologically active cytokines作者: guardianship 時(shí)間: 2025-3-29 04:44 作者: FIR 時(shí)間: 2025-3-29 10:26 作者: paleolithic 時(shí)間: 2025-3-29 13:22 作者: 莎草 時(shí)間: 2025-3-29 15:56 作者: 危險(xiǎn) 時(shí)間: 2025-3-29 19:49 作者: BILK 時(shí)間: 2025-3-30 02:20 作者: Admire 時(shí)間: 2025-3-30 06:45
A Useful Guide for Analysis of Immune Markers by Fluorochrome (Luminex) Techniquelear cells (PBMC)..This chapter describes the Luminex technique for detection of multiple cytokines by magnetic bead sandwich immunoassay, with a special focus on some important pre-analytical factors, such as cell separation, cryopreservation, and PBMC thawing that may affect the detection outcome 作者: Noisome 時(shí)間: 2025-3-30 11:57
An In Vitro One-Dimensional Assay to Study Growth Factor-Regulated Tumor Cell–Macrophage Interactionaling pathways involved in the macrophage–tumor cell interaction. This high-resolution motility assay will lead to a more detailed description of immune cell–tumor cell behavior as well as interrogating additional cell types within the tumor microenvironment which use cytokine/growth factor paracrin作者: 機(jī)構(gòu) 時(shí)間: 2025-3-30 12:54 作者: 鼓掌 時(shí)間: 2025-3-30 18:26 作者: 過時(shí) 時(shí)間: 2025-3-30 21:02 作者: Graphite 時(shí)間: 2025-3-31 04:36
Milos Dordevic,Michael T. Hirschmann MDmposed of multi-walled carbon nanotubes with carboxylic acid functionalities (multi-walled carbon nanotubes–COOH) to increase the surface area for the range of human IL-10 detection. These were activated with .-hydroxysuccinimide and .-(3-dimethylaminopropyl)-.′-ethyl-carbodiimide hydrochloride for 作者: 生氣地 時(shí)間: 2025-3-31 08:10
Milos Dordevic,Michael T. Hirschmann MD an overview of cytometric bead array technology, introduces some of the kits, and provides detailed information about the one that performed well in a comparative study (Cytokine Human Magnetic 30-Plex Panel from Life Technologies?).作者: Colonnade 時(shí)間: 2025-3-31 09:34
Anterior Cruciate Ligament Reconstructionlear cells (PBMC)..This chapter describes the Luminex technique for detection of multiple cytokines by magnetic bead sandwich immunoassay, with a special focus on some important pre-analytical factors, such as cell separation, cryopreservation, and PBMC thawing that may affect the detection outcome 作者: neurologist 時(shí)間: 2025-3-31 13:33
Anterior knee pain and patellar instabilityaling pathways involved in the macrophage–tumor cell interaction. This high-resolution motility assay will lead to a more detailed description of immune cell–tumor cell behavior as well as interrogating additional cell types within the tumor microenvironment which use cytokine/growth factor paracrin作者: Bronchial-Tubes 時(shí)間: 2025-3-31 20:15 作者: 懦夫 時(shí)間: 2025-3-31 22:53
1064-3745 , plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELIS978-1-4939-4850-5978-1-4939-0928-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AUGER 時(shí)間: 2025-4-1 04:08 作者: COWER 時(shí)間: 2025-4-1 09:36
Analysis of the Released Nuclear Cytokine HMGB1 in Human Serumcells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
