作者: OWL 時(shí)間: 2025-3-21 21:21 作者: 事與愿違 時(shí)間: 2025-3-22 02:29
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242617.jpg作者: Motilin 時(shí)間: 2025-3-22 07:25
Hans Peter Latscha,Helmut Alfons KleinA general procedure for the solubilization of cytochrome P450 (P450) from bacterial membranes specifically for a human P450 expressed heterologously in the host . co. is described. The example involves the use of a P450 (3A4) with a C-terminal oligohistidine tag and includes sequential DEAE and metal affinity chromatography.作者: 殖民地 時(shí)間: 2025-3-22 09:08 作者: 編輯才信任 時(shí)間: 2025-3-22 13:16
https://doi.org/10.1007/978-3-86226-287-8nes. The nomenclature is presented as a unifying principle to enhance communication across disciplines. Because of the historical nature of gene sequencing, sometimes names have to be changed, but this is kept to a bare minimum to avoid confusion in the literature. CYP names have now reached four di作者: 編輯才信任 時(shí)間: 2025-3-22 17:05 作者: 排他 時(shí)間: 2025-3-22 21:40 作者: 搜集 時(shí)間: 2025-3-23 04:38
Hans Peter Latscha,Helmut Alfons Kleinbolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. Methods are described for the generation of recombinant baculoviral DNAs, via both site-specific tran作者: 類(lèi)型 時(shí)間: 2025-3-23 05:39
Hans Peter Latscha,Helmut Alfons Kleinals. Individual P450 forms have unique, but overlapping, substrate specificities. It is necessary to determine which P450s play more important roles in the oxidation of these chemicals. A good way of studying the roles of P450s in the metabolism of these chemicals is to reconstitute the activities b作者: Custodian 時(shí)間: 2025-3-23 13:19 作者: defendant 時(shí)間: 2025-3-23 16:20
Hans Peter Latscha,Helmut Alfons Klein and play a major role in the metabolism of many foreign chemicals and endogenous substances. We describe a spectrofluorometric method for determining 7-ethoxyresorufin .-dealkylation catalyzed by CYP1A1, CYP1A2, and CYP1B1. The formation of the enzymatic product, resorufin, is monitored continuousl作者: savage 時(shí)間: 2025-3-23 19:56
Hans Peter Latscha,Helmut Alfons Kleinmixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hyd作者: Petechiae 時(shí)間: 2025-3-24 00:47 作者: Asparagus 時(shí)間: 2025-3-24 03:29
Hans Peter Latscha,Helmut Alfons Kleinivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical sepa作者: 象形文字 時(shí)間: 2025-3-24 09:49 作者: 得意牛 時(shí)間: 2025-3-24 12:56 作者: 施魔法 時(shí)間: 2025-3-24 18:23
Hans Peter Latscha,Helmut Alfons Kleinincubation of bufuralol with cDNA-expressed CYP2D6 or human liver microsomes. Analytical separation is achieved using a C. column and a mobile phase consisting of 30% acetonitrile and 2 m. perchloric acid, with detection by fluorescence using an excitation wavelength of 252 nm and an emission wavele作者: 附錄 時(shí)間: 2025-3-24 23:04 作者: 組成 時(shí)間: 2025-3-25 01:16
Hans Peter Latscha,Helmut Alfons KleinCYP3A enzymes from various species, including human, metabolize testosterone by a 6β-hydroxylation reaction, which is unique to this P450 subfamily. A thin-layer chromatographic method is described for the determination of 6β-hydroxytestosterone formed enzymatically by incubation of [.C]-testosteron作者: 陳舊 時(shí)間: 2025-3-25 06:11 作者: saturated-fat 時(shí)間: 2025-3-25 09:50 作者: 惡意 時(shí)間: 2025-3-25 13:09
Komplexverbindungen Bindungen in Komplexenonitor P450 (P450) activity in both hepatic and extrahepatic tissues. A spectrofluorometric method is described for determination of P450-catalyzed 7-ethoxycoumarin .-deethylation. Following acidification of the incubation mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-e作者: 銼屑 時(shí)間: 2025-3-25 17:05
Cytochrome P450 Protocols978-1-59259-998-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AGATE 時(shí)間: 2025-3-25 22:44
Hans Peter Latscha,Helmut Alfons Klein microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.作者: 河流 時(shí)間: 2025-3-26 03:22
,Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatogr microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.作者: OPINE 時(shí)間: 2025-3-26 05:49 作者: 冷峻 時(shí)間: 2025-3-26 09:16 作者: 形容詞詞尾 時(shí)間: 2025-3-26 14:54 作者: 和平主義 時(shí)間: 2025-3-26 17:15
Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System,bolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. Methods are described for the generation of recombinant baculoviral DNAs, via both site-specific tran作者: carbohydrate 時(shí)間: 2025-3-26 23:34 作者: 共同時(shí)代 時(shí)間: 2025-3-27 01:18 作者: gratify 時(shí)間: 2025-3-27 05:18 作者: cyanosis 時(shí)間: 2025-3-27 12:28
Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation,mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hyd作者: 四目在模仿 時(shí)間: 2025-3-27 13:40
Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin ,-Deethylation Activity in ide, and may be an important determinant of clinical responses to these agents. A spectrofluorometric method is described for the determination of CYP2B6-catalyzed 7-ethoxy-4-trifluoromethylcoumarin .-deethylation activity in human liver microsomes. The specificity of this method for CYP2B6 is incre作者: AMOR 時(shí)間: 2025-3-27 17:49
,High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation,ivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical sepa作者: Congestion 時(shí)間: 2025-3-27 22:09
,Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatogr microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed d作者: 施舍 時(shí)間: 2025-3-28 05:50
,CYP2C19-Mediated (,)-Mephenytoin 4′-Hydroxylation Assayed by High-Performance Liquid Chromatography.C-labeled (.)-mephenytoin following incubation with cDNA-expressed CYP2C19 or human liver microsomes. Analytical separation is achieved using a C. column developed with a gradient from 10 to 100% methanol, with detection using a scintillation detector. This method is applicable to enzymatic studies作者: neurologist 時(shí)間: 2025-3-28 08:15 作者: Flavouring 時(shí)間: 2025-3-28 11:50 作者: Barter 時(shí)間: 2025-3-28 17:09 作者: insincerity 時(shí)間: 2025-3-28 22:38
Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatos described for the quantification of 12-hydroxylauric acid formed enzymatically by incubation of .C-labeled lauric acid with cDNA-expressed CYP4A11 or human liver microsomes. Analytical separation is achieved using a C. column and a gradient of 30% acetonitrile and 2 m. perchloric acid to 100% meth作者: antipsychotic 時(shí)間: 2025-3-29 00:19 作者: AVOW 時(shí)間: 2025-3-29 04:52
Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families,onitor P450 (P450) activity in both hepatic and extrahepatic tissues. A spectrofluorometric method is described for determination of P450-catalyzed 7-ethoxycoumarin .-deethylation. Following acidification of the incubation mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-e作者: 使長(zhǎng)胖 時(shí)間: 2025-3-29 09:30
1064-3745 ssential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and th作者: 面包屑 時(shí)間: 2025-3-29 11:28
Hans Peter Latscha,Helmut Alfons Kleinent. This chapter provides a detailed technical description of the processes that allow the coexpression of various CYP isoforms together with CYP reductase in . and gives some examples of the results that can be achieved for the expression of human P450s.作者: Prosaic 時(shí)間: 2025-3-29 17:12
Hans Peter Latscha,Helmut Alfons Kleinand an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hydroxylation activity in microsomes prepared from liver and other tissues and in isolated hepatocytes and cultured cells that express this cytochrome P450 activity.作者: definition 時(shí)間: 2025-3-29 22:48 作者: 誓言 時(shí)間: 2025-3-30 00:24
Hans Peter Latscha,Helmut Alfons Kleinonsisting of 30% acetonitrile and 2 m. perchloric acid, with detection by fluorescence using an excitation wavelength of 252 nm and an emission wavelength of 302 nm. This method is applicable to enzymatic studies for determination of CYP2D6-catalyzed bufuralol 1′-hydroxylation activity.作者: Recessive 時(shí)間: 2025-3-30 06:18 作者: Grating 時(shí)間: 2025-3-30 10:00 作者: MAL 時(shí)間: 2025-3-30 15:09
Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in ,,ent. This chapter provides a detailed technical description of the processes that allow the coexpression of various CYP isoforms together with CYP reductase in . and gives some examples of the results that can be achieved for the expression of human P450s.作者: 和音 時(shí)間: 2025-3-30 18:01
Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation,and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hydroxylation activity in microsomes prepared from liver and other tissues and in isolated hepatocytes and cultured cells that express this cytochrome P450 activity.作者: 吸引力 時(shí)間: 2025-3-30 22:38 作者: 徹底檢查 時(shí)間: 2025-3-31 02:06
,CYP2D6-Dependent Bufuralol 1′-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquonsisting of 30% acetonitrile and 2 m. perchloric acid, with detection by fluorescence using an excitation wavelength of 252 nm and an emission wavelength of 302 nm. This method is applicable to enzymatic studies for determination of CYP2D6-catalyzed bufuralol 1′-hydroxylation activity.作者: AXIS 時(shí)間: 2025-3-31 05:27 作者: 開(kāi)始沒(méi)有 時(shí)間: 2025-3-31 11:46 作者: SOB 時(shí)間: 2025-3-31 16:47
Hans Peter Latscha,Helmut Alfons Kleinsposition in . and site-specific recombination in vitro; adaptation of . (.) 9 cells to shaking culture and to serum-free medium; cryopreservation and transfection of .9 cells; amplification of baculovirus and determination of viral titer; analysis of baculoviral DNA; and expression and analysis of recombinant proteins.作者: 侵蝕 時(shí)間: 2025-3-31 20:37
Hans Peter Latscha,Helmut Alfons Kleinrations are achieved using a C. column with a linear gradient of 10–100% methanol, with detection at 230 nm. This method is applicable to enzymatic studies for determination of CYP2C8-catalyzed paclitaxel 6α-hydroxylation activity.作者: 價(jià)值在貶值 時(shí)間: 2025-4-1 00:25 作者: GRIPE 時(shí)間: 2025-4-1 04:52 作者: 諂媚于性 時(shí)間: 2025-4-1 06:28 作者: 無(wú)價(jià)值 時(shí)間: 2025-4-1 11:45
Hans Peter Latscha,Helmut Alfons Kleinrmation of resorufin from other alkoxyresorufins, such as 7-methoxyresorufin, 7-benzyloxyresorufin, and 7-pentoxyresorufin. It can also be used to assay 7-ethoxyresorufin .-dealkylation activity in isolated hepatocytes and cultured cells that express this P450 activity.作者: stroke 時(shí)間: 2025-4-1 17:47
Hans Peter Latscha,Helmut Alfons Kleinmal metabolism of 7-ethoxy-4-trifluoromethylcoumarin. The enzymatic product, 7-hydroxy-4-trifluoromethylcoumarin, is monitored by fluorescence using an excitation wavelength of 410 nm and an emission wavelength of 510 nm. This approach can be modified to assay the catalytic activity of cDNA-expressed CYP2B6.作者: synovium 時(shí)間: 2025-4-1 20:03
Komplexverbindungen Bindungen in Komplexenudies to determine the catalytic activity of cDNA-expressed human enzymes in the CYP1, CYP2, and CYP3 families, and 7-ethoxycoumarin .-deethylation activity in microsomes isolated from liver and other tissues.
