標(biāo)題: Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 19981st edition Springer Science+Business Media New York 1998 [打印本頁] 作者: MASS 時(shí)間: 2025-3-21 16:59
書目名稱Cytochrome P450 Protocols影響因子(影響力)
作者: 揭穿真相 時(shí)間: 2025-3-21 20:30 作者: 噱頭 時(shí)間: 2025-3-22 01:11
Spectral Analyses of Cytochromes P450, heme found in b-type cytochromes, like cytochrome b, hemoglobin, and myoglobm. This type of heme prosthetic group can be removed from the protein by acidic acetone or by alkali and pyridine. There are a number of methods that can be used for spectrophotometrrc analysis of hemoproteins, each useful 作者: gene-therapy 時(shí)間: 2025-3-22 07:56
Purification of Cytochromes P450,quisite to many types of investigation, purified enzymes are still required for studies on structure and mechanism. Purified enzymes are also needed for preparation of antibodies, as standards in assays, and for other purposes that may often be overlooked.作者: paradigm 時(shí)間: 2025-3-22 09:49 作者: FECK 時(shí)間: 2025-3-22 16:38 作者: FECK 時(shí)間: 2025-3-22 18:53 作者: Oratory 時(shí)間: 2025-3-22 21:34
Cytochrome P450 Reconstitution Systems,ucturally diverse xenobrotrc chemicals such as drugs, toxic chemicals, and carcinogens, as well as endobiottc chemicals including steroids, fatty acids, fat-soluble vitamins, and prostaglandins (.). Major cytochrome P450 enzymes in human liver microsomes identified to date mclude CYPlA2,2A6,2B6,2C8,作者: DEI 時(shí)間: 2025-3-23 02:23
Catalytic Assays for Human Cytochrome P450,ny P450 enzymes have been isolated and identified in liver and other tlssues, including kidney, lung, intestines, and brain. Although these enzymes have a high degree of similarity in their ammo-acid sequences, many of them are subject to differential regulation and have distinct catalytic functions作者: precede 時(shí)間: 2025-3-23 09:20
Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Suber (., .), but its expression is readily detectable in lung (., .). By contrast, CYP1A2 is constitutively expressed in human liver (., .) and is absent in lung (., .). CYP1B1 is primarily an extrahepatic P450, as suggested by the findmg that CYP1B1 mRNA is present in much greater abundance in tissue作者: 不容置疑 時(shí)間: 2025-3-23 13:42
Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation,otal hepatic P450 content) (.), although substantial intermdividual variation exists (.–.). This P450 is primarily a hepatic protein, as suggested by its absence from several extrahepatic tissues, including adult human lung, colon, breast, kidney, and placenta microsomes (.). According to experiment作者: JEER 時(shí)間: 2025-3-23 14:42 作者: 忍耐 時(shí)間: 2025-3-23 20:12
,High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6α-Hydroxylation, content (.) and a blmodal dlstrlbutlon in CYP2C8 protein amounts m a panel of human liver mlcrosomes has been reported (.). Experiments with primary cultures of human hepatocytes have indicated that CYP2C8 is subject to inductton by phenobarbital, dexamethasone, and rifampin (rifampicm) (.). Little作者: excrete 時(shí)間: 2025-3-24 00:07 作者: Harridan 時(shí)間: 2025-3-24 02:31 作者: filial 時(shí)間: 2025-3-24 06:38 作者: Gerontology 時(shí)間: 2025-3-24 11:39 作者: Capitulate 時(shí)間: 2025-3-24 17:31 作者: 相互影響 時(shí)間: 2025-3-24 22:48 作者: Anhydrous 時(shí)間: 2025-3-25 00:28
1064-3745 estigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification o作者: vector 時(shí)間: 2025-3-25 07:14 作者: 一個(gè)攪動(dòng)不安 時(shí)間: 2025-3-25 08:16 作者: 和平主義 時(shí)間: 2025-3-25 13:48 作者: Indicative 時(shí)間: 2025-3-25 15:54 作者: 步兵 時(shí)間: 2025-3-25 23:42
Book 19981st edition of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene exp作者: Immunotherapy 時(shí)間: 2025-3-26 01:50
Cytochrome P450 Protocols978-1-59259-580-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Preamble 時(shí)間: 2025-3-26 07:16
P. Yang,A. M. Esper,G. S. Martinquisite to many types of investigation, purified enzymes are still required for studies on structure and mechanism. Purified enzymes are also needed for preparation of antibodies, as standards in assays, and for other purposes that may often be overlooked.作者: prosthesis 時(shí)間: 2025-3-26 09:36 作者: flourish 時(shí)間: 2025-3-26 13:06
P. L. Silva,P. R. M. Rocco,P. Pelosit must be mastered if one is to optimize new methods when seeking answers to specific questlons. The hlstory of cytochrome P450 (P450) research is intimately woven with seminal discoveries resulting from the development and application of new methodologies and new instruments. It is the purpose of t作者: chastise 時(shí)間: 2025-3-26 17:10
A. H. Jonkman,H. J. de Vries,L. M. A. Heunksytochrome P450 (P450), there are more than 750 sequences and they are accumulating rapidly. At the current rate, there will be more than 1000 P450 sequences by late 1998 or early 1999. For these data to be accessible and useful they must be sorted and categorized in some meaningful way. To that end,作者: 思考才皺眉 時(shí)間: 2025-3-27 00:21 作者: 圓錐體 時(shí)間: 2025-3-27 02:33 作者: 同位素 時(shí)間: 2025-3-27 09:10 作者: Basal-Ganglia 時(shí)間: 2025-3-27 10:54 作者: ineluctable 時(shí)間: 2025-3-27 15:25 作者: syring 時(shí)間: 2025-3-27 20:47
https://doi.org/10.1007/978-3-030-73231-8ucturally diverse xenobrotrc chemicals such as drugs, toxic chemicals, and carcinogens, as well as endobiottc chemicals including steroids, fatty acids, fat-soluble vitamins, and prostaglandins (.). Major cytochrome P450 enzymes in human liver microsomes identified to date mclude CYPlA2,2A6,2B6,2C8,作者: 秘傳 時(shí)間: 2025-3-28 01:52
Z. Ltaief,A. G. Schneider,L. Liaudetny P450 enzymes have been isolated and identified in liver and other tlssues, including kidney, lung, intestines, and brain. Although these enzymes have a high degree of similarity in their ammo-acid sequences, many of them are subject to differential regulation and have distinct catalytic functions作者: Ingratiate 時(shí)間: 2025-3-28 04:55 作者: folliculitis 時(shí)間: 2025-3-28 10:10
S. N. Myatra,N. Prabu,J.-L. Teboulotal hepatic P450 content) (.), although substantial intermdividual variation exists (.–.). This P450 is primarily a hepatic protein, as suggested by its absence from several extrahepatic tissues, including adult human lung, colon, breast, kidney, and placenta microsomes (.). According to experiment作者: 沒血色 時(shí)間: 2025-3-28 12:32 作者: dura-mater 時(shí)間: 2025-3-28 15:43 作者: incite 時(shí)間: 2025-3-28 20:30
Fluid Management in COVID-19 ICU Patients9 protein expression can be increased in cells treated with a P450 inducer such as phenobarbital, dexamethasone, or rlfampin (rifampicin) (.). This is consistent with the clmical finding that rlfampin increases the total body clearance of tolbutamide (.), which is metabolized almost entirely by hepa作者: 反對(duì) 時(shí)間: 2025-3-29 00:24
Precision Medicine in Septic Shockher CYP2C8 or CYP2C9 (.). Considerable interindividual differences occur in hepatic CYP2C19 content (., .), primarily owmg to a genetic polymorphism in this enzyme (4). Approximately 15-20% of Orientals and 3% of Caucasians exhibit the CYP2C19 poor-metabolizer phenotype, which is associated with at 作者: 發(fā)酵劑 時(shí)間: 2025-3-29 04:37
P. Eggimann,Y. -A. Que,F. Venturasulting in a substantially reduced capacity to metabolize a large number of clinically useful drugs, including metoprolol, propafenone, haloperrdol, dextromethorphan and codeine (.). Quinidine competrtively inhibits CYP2D6, with a K. of 3-30 m. (.–.). Because of its preferential inhibition of CYP2D6作者: macular-edema 時(shí)間: 2025-3-29 09:12
O. Turgman,M. Schinkel,W. J. Wiersingacultures of human hepatocytes with ethanol induces CYP2E1 protein (.) and this is consistent with the finding that hepatic CYP2El protein (.) and mRNA (.) amounts are increased in alcoholics. Although only a few drugs (e.g., acetaminophen [.]) have been identified as substrates for CYP2E1, many low 作者: 確定的事 時(shí)間: 2025-3-29 15:28
A. Abdalla,K. Dhaliwal,M. Shankar-Haris expressed in human fetal liver (.). The CYP3A enzymes account for an estimated ~30% of total human cytochrome P450 content in adult liver (.), although large inter-individual differences exist in hepatic CYP3A content. CYP3A4 is present in all adult human livers and is inducible by drugs such as r作者: Hippocampus 時(shí)間: 2025-3-29 17:30
Coral Spawning Behavior and Timing4All mRNA is present in greater abundance in kidney than in liver, whereas it is absent in lung (.). Various fatty acids, including lauric acid (.–.), are substrates for recombinant CYP4Al l. Experiments with a panel of individual cDNA-expressed P450 enzymes indicate that CYP4Al l is a major lauric 作者: A簡潔的 時(shí)間: 2025-3-29 21:34 作者: 釋放 時(shí)間: 2025-3-30 00:26
Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems,plasmid expressed (NF14) are presented elsewhere (., .). The general procedure can be utilized for other cytochromes P450, with some modification, as described elsewhere (., .–.). Several general purification techniques are described in detarl in Chapters 4 and 5 of this volume.作者: blight 時(shí)間: 2025-3-30 07:55
Springer Science+Business Media New York 1998作者: 未成熟 時(shí)間: 2025-3-30 09:24 作者: Abduct 時(shí)間: 2025-3-30 15:59
Book 19981st edition this exhaustive collection of core techniques...Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharma作者: 你正派 時(shí)間: 2025-3-30 18:54 作者: 偏見 時(shí)間: 2025-3-30 23:23
A. H. Jonkman,H. J. de Vries,L. M. A. Heunksmembership. Since the inception of the system, both numbers have crept downward. The actual decision to mclude a sequence in an existing group largely depends on how it clusters on a tree and not so much on the absolute percent identity, which is more or less a rule of thumb. The most recent publish作者: Cholesterol 時(shí)間: 2025-3-31 02:19
P. Yang,A. M. Esper,G. S. Martine the microsomal proteins (.). Various induced forms of rat cytochrome P450 were likewise detergent-solubrhzed, using sodium cholate, and polyethylene glycol fractionated, and subjected to DEAE-chromatography with the added step of hydroxylapatite chromatography in the presence of nomonic detergent 作者: Metamorphosis 時(shí)間: 2025-3-31 05:39
G. Hernandez,M. Brenner,B. A. McGrath sensitrvity for detection of an individual form of cytochrome P450 is 0.5 pmol/lane. In the case of tissues in which the cytochrome P450 content is as low as 1 pmol/mg, even if all of the cytochrome P450 were a single form, it would be necessary to load 0.5 mg microsomal protein per lane in order t作者: 使痛苦 時(shí)間: 2025-3-31 11:52 作者: Irrepressible 時(shí)間: 2025-3-31 13:45
Z. Ltaief,A. G. Schneider,L. Liaudet expenmental tools in a variety of studies. These include studies designed: 1 To identify individual P45Os that catalyze the actlvatlon or detoxification of a particular compound, 2 To quantify the expression and catalytic activity of an mdlvrdual P450 in tissue samples and In cultured cells, 3. To 作者: indemnify 時(shí)間: 2025-3-31 20:34 作者: Occipital-Lobe 時(shí)間: 2025-4-1 01:03
S. N. Myatra,N. Prabu,J.-L. Teboul is active at only ～5% the rate of CYP2A6 (.). Immunoinhibition studies have validated the use of coumarm 7-hydroxylase activity as a diagnostic catalytic marker for human hepatic CYP2A6. Antihuman CYP2A6 IgG inhibits coumarm 7-hydroxylase activity by >90% in human liver microsomes (.), indicating t作者: Aromatic 時(shí)間: 2025-4-1 05:44
Fluid Management in COVID-19 ICU Patients. However, little is known regardmg the role of this P450 in drug metabolism, although cDNA-expressed CYP2B6 is an active catalyst of lidocaine .-deethylatlon (.) and human hver microsomal CYP2B6 appears to be a high-Km catalyst of cyclophosphamlde 4-hydroxylation (.). Detailed mvestlgations of hepa作者: Complement 時(shí)間: 2025-4-1 09:17
P. L. Silva,P. R. M. Rocco,P. PelosiYPlA2,2A6, 2B6,2C8, 2C9-Ile359, 2C9-Cys’44, 2C18, 2C19, 2D6, 2EI,3A3,3A4 and 3A5 are inactive (.–.). Paclitaxel Ga-hydroxylase actlvlty may therefore be a potentially useful dlagnostlc catalytic marker for human hepatic CYP2C8 ... This chapter describes a high-performance liquid chromatographic (HPL作者: 建筑師 時(shí)間: 2025-4-1 12:17 作者: canvass 時(shí)間: 2025-4-1 18:21 作者: 龍蝦 時(shí)間: 2025-4-1 20:53
