標(biāo)題: Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.Genomics.H [打印本頁] 作者: Agoraphobia 時(shí)間: 2025-3-21 19:53
書目名稱Cystic Fibrosis影響因子(影響力)
書目名稱Cystic Fibrosis影響因子(影響力)學(xué)科排名
書目名稱Cystic Fibrosis網(wǎng)絡(luò)公開度
書目名稱Cystic Fibrosis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cystic Fibrosis被引頻次
書目名稱Cystic Fibrosis被引頻次學(xué)科排名
書目名稱Cystic Fibrosis年度引用
書目名稱Cystic Fibrosis年度引用學(xué)科排名
書目名稱Cystic Fibrosis讀者反饋
書目名稱Cystic Fibrosis讀者反饋學(xué)科排名
作者: 神刊 時(shí)間: 2025-3-21 22:27 作者: Orchiectomy 時(shí)間: 2025-3-22 02:56 作者: anagen 時(shí)間: 2025-3-22 06:40
Book 2011the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Comprehensive and practical, .Cystic Fibrosis: Diagnosis and Protocols. will provide readers with optimal working tools to address pressing questi作者: Nmda-Receptor 時(shí)間: 2025-3-22 12:27
Introduction to Section II: Omics in the Biology of Cystic Fibrosisand CF pathophysiology, a new era of technologies and methodologies focused on systems-level approaches to analysis of cell and tissue function has emerged. These technologies focus our understanding on the environment supporting protein function (referred to genomics) and the protein composition of作者: Substance 時(shí)間: 2025-3-22 15:52 作者: Substance 時(shí)間: 2025-3-22 19:15
Introduction to Section I: Overview of Approaches to Study Cystic Fibrosis Pathophysiologyological changes occurring in CF lung disease with the mutation of a chloride channel is ongoing. The failure of innate lung defense in CF, and the subsequent cyclical microbial colonization of airways, explains the gross anatomical changes that occur in CF pathophysiology. However, ongoing research作者: 刪減 時(shí)間: 2025-3-22 23:05
Imaging CFTR Protein Localization in Cultured Cells and Tissuescommonly used to assess the subcellular localization and relative abundance of CFTR. Visualization of heterologously overexpressed CFTR is typically unproblematic and straightforward, whereas detection of small quantities of endogenous CFTR in tissues can be challenging and requires highly specific 作者: cutlery 時(shí)間: 2025-3-23 01:41 作者: Hallmark 時(shí)間: 2025-3-23 06:51 作者: 使成整體 時(shí)間: 2025-3-23 10:14
Methods for ASL Measurements and Mucus Transport Rates in Cell Culturesuce and are bathed by a layer of airway surface liquid (ASL), which plays a critical role in lung defense by helping to maintain the sterility of the lung. This layer is composed of two phases: the mucus layer which functions to trap particulates, bacteria, and viruses, and the underlying periciliar作者: ITCH 時(shí)間: 2025-3-23 15:53 作者: PET-scan 時(shí)間: 2025-3-23 18:30
Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibfor G protein-coupled receptors (GPCR). Intracellular calcium (Ca..) mobilization in airway epithelia in response to extracellular stimuli regulates key airway innate defense functions, e.g., Ca.-activated Cl. secretion, ciliary beating, mucin secretion, and inflammatory responses. Because Ca.. mobi作者: HUMP 時(shí)間: 2025-3-23 23:20 作者: 止痛藥 時(shí)間: 2025-3-24 03:39 作者: mosque 時(shí)間: 2025-3-24 10:35 作者: Incorruptible 時(shí)間: 2025-3-24 11:57 作者: endarterectomy 時(shí)間: 2025-3-24 15:02
Microarray mRNA Expression Profiling to Study Cystic Fibrosisaffected cell types. Microarray expression profiling provides a platform to study global gene expression in detail. This approach may provide the necessary information to segregate phenotypic characteristics of CF, differentiate between genetic or environmental factors, and assess the advent and pro作者: Ige326 時(shí)間: 2025-3-24 19:55 作者: ALE 時(shí)間: 2025-3-25 01:02 作者: 衰弱的心 時(shí)間: 2025-3-25 06:52 作者: thalamus 時(shí)間: 2025-3-25 10:16 作者: aptitude 時(shí)間: 2025-3-25 15:23
Imaging CFTR Protein Localization in Cultured Cells and Tissuesantibodies and optimized staining protocols. CFTR tagged by green fluorescent protein can be employed to study trafficking in live cells. Tagging of CFTR with an extracellular epitope permits detection exclusively at the cell surface and subsequent chasing allows visualization of endocytic trafficking.作者: minimal 時(shí)間: 2025-3-25 17:41 作者: 生命層 時(shí)間: 2025-3-25 21:49 作者: foliage 時(shí)間: 2025-3-26 01:26 作者: 為現(xiàn)場 時(shí)間: 2025-3-26 07:31
Animation, Embodiment, and Digital Media cells on microporous membrane at the air–liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir?.作者: 沒有希望 時(shí)間: 2025-3-26 11:39 作者: Admonish 時(shí)間: 2025-3-26 13:00
Problem Statement and Preliminariesding this link, and this series of chapters details the recent progress that has been made in understanding the interplay between ion transport activity and innate lung defense, and the initiation of CF lung pathophysiology.作者: obtuse 時(shí)間: 2025-3-26 18:34 作者: 提煉 時(shí)間: 2025-3-26 23:12 作者: 埋葬 時(shí)間: 2025-3-27 02:16 作者: 討好美人 時(shí)間: 2025-3-27 05:29
Channel Surfers: ,’s Postnational Fansf treatment. We provide here detailed protocols and tips for collecting and preserving tissues and cells, and preparing total RNA. We also outline novel strategies for experimental design and data analysis, and describe some powerful gene and pathway discovery tools.作者: 不知疲倦 時(shí)間: 2025-3-27 11:07 作者: Heterodoxy 時(shí)間: 2025-3-27 15:47 作者: CRUC 時(shí)間: 2025-3-27 20:49 作者: Gustatory 時(shí)間: 2025-3-27 23:37
Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibathogenesis of CF airway disease. Hence, assessment of Ca.. signaling has become an important area of CF research. This chapter will focus on measurements of cytoplasmic and mitochondrial Ca. signals resulting from GPCR activation in polarized primary cultures of normal and CF human bronchial epithelia (HBE).作者: Nerve-Block 時(shí)間: 2025-3-28 02:24 作者: 無動(dòng)于衷 時(shí)間: 2025-3-28 07:54 作者: Chivalrous 時(shí)間: 2025-3-28 13:14 作者: 完成才能戰(zhàn)勝 時(shí)間: 2025-3-28 16:34
Identification and Quantification of Mucin Expressioncial considerations and methodology. We describe methods for the general quantification of heavily glycosylated proteins as the mucins using dot/slot blot. We also describe the separation of the mucins by gel electrophoresis and the identification with specific antibodies on Western blot and by proteomics.作者: 箴言 時(shí)間: 2025-3-28 21:37
Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells cells on microporous membrane at the air–liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir?.作者: IRS 時(shí)間: 2025-3-29 02:40 作者: 公社 時(shí)間: 2025-3-29 06:24
Problem Statement and Preliminariesantibodies and optimized staining protocols. CFTR tagged by green fluorescent protein can be employed to study trafficking in live cells. Tagging of CFTR with an extracellular epitope permits detection exclusively at the cell surface and subsequent chasing allows visualization of endocytic trafficking.作者: 愛管閑事 時(shí)間: 2025-3-29 11:01
Anime from Akira to Princess Mononokely involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.作者: abstemious 時(shí)間: 2025-3-29 15:02
Book 2011rasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.? .Cystic Fibrosis: Diagnosis and Protocols. aims to provide the CF research community and related researchers with a very wide range of high作者: 未開化 時(shí)間: 2025-3-29 18:39
Video-Based Performance Captureher transporters. The modulation of epithelial Na. channel (ENaC) function serves as a prime example of regulatory function of the CFTR. In this chapter, we will briefly describe an integrated protocol consisting of biochemical and electrophysiological approaches to study the regulation of ENaC by CFTR.作者: 鬼魂 時(shí)間: 2025-3-29 23:25
Animation und Interaktion im WWWlso demonstrate that these methods are also applicable in novel ways to probe the ultrastructure of the airways including the establishment of pH gradients and the ability of the apical membrane glycocalyx in excluding larger molecules from the cell surface.作者: deforestation 時(shí)間: 2025-3-30 00:37
https://doi.org/10.1007/978-3-642-48790-3ral analysis of secretion rates. The composition and properties of the mucus (e.g., solids, viscosity, pH) can also be determined. These methods have now been applied to mice, ferrets, cats, pigs, sheep, and humans, with a main goal of comparing gland secretion in control and CFTR-deficient humans and animals.作者: Herbivorous 時(shí)間: 2025-3-30 07:00 作者: 致命 時(shí)間: 2025-3-30 10:36
Methods for ASL Measurements and Mucus Transport Rates in Cell Cultureslso demonstrate that these methods are also applicable in novel ways to probe the ultrastructure of the airways including the establishment of pH gradients and the ability of the apical membrane glycocalyx in excluding larger molecules from the cell surface.作者: 粗俗人 時(shí)間: 2025-3-30 15:20 作者: micronutrients 時(shí)間: 2025-3-30 20:12 作者: 群居男女 時(shí)間: 2025-3-30 22:39
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242595.jpg作者: –吃 時(shí)間: 2025-3-31 04:43
https://doi.org/10.1007/978-1-61779-120-8CF; Genomics; Human clinical trials; Lipidomics; Pathophysiology; Proteomics作者: 空中 時(shí)間: 2025-3-31 06:10 作者: 空洞 時(shí)間: 2025-3-31 10:56 作者: travail 時(shí)間: 2025-3-31 13:42
