標題: Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.CFTR expre [打印本頁] 作者: 鏟除 時間: 2025-3-21 16:31
書目名稱Cystic Fibrosis影響因子(影響力)
書目名稱Cystic Fibrosis影響因子(影響力)學科排名
書目名稱Cystic Fibrosis網(wǎng)絡公開度
書目名稱Cystic Fibrosis網(wǎng)絡公開度學科排名
書目名稱Cystic Fibrosis被引頻次
書目名稱Cystic Fibrosis被引頻次學科排名
書目名稱Cystic Fibrosis年度引用
書目名稱Cystic Fibrosis年度引用學科排名
書目名稱Cystic Fibrosis讀者反饋
書目名稱Cystic Fibrosis讀者反饋學科排名
作者: 蒸發(fā) 時間: 2025-3-21 23:12 作者: cardiac-arrest 時間: 2025-3-22 02:20 作者: incontinence 時間: 2025-3-22 07:57 作者: 高歌 時間: 2025-3-22 09:21 作者: CLAN 時間: 2025-3-22 13:50
In Vitro Methods for CFTR Biogenesisgth CFTR expression can be markedly increased using mRNA lacking a 5.-cap analog (G(5.)ppp(5.)G), whereas the reverse usually holds for smaller proteins and individual CFTR domains. In the context of the full-length mRNA, translation was further stimulated by the presence of a long 3.-UTR. The secon作者: CLAN 時間: 2025-3-22 19:04
Book 2011ons to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Comprehensive and practical, .Cystic Fibrosis: Diagnosis and Protocols. will provide readers with optimal作者: TEM 時間: 2025-3-22 21:46
1064-3745 p, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Comprehensive and practical, .Cystic Fibrosis: Diagnosis and Protocols. will provide readers with optimal978-1-4939-5787-3978-1-61779-117-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Interstellar 時間: 2025-3-23 02:09 作者: 蚊子 時間: 2025-3-23 06:17 作者: 富饒 時間: 2025-3-23 10:24 作者: Dedication 時間: 2025-3-23 13:58 作者: 故意釣到白楊 時間: 2025-3-23 18:42
Design of Gene Therapy Trials in CF Patientsnon-viral gene transfer agents. These have largely been single dose to either nose or lower airway and have been designed around molecular or bioelectrical outcome measures. Both transgene mRNA and partial correction of chloride secretion have been reported, although sodium hyperabsorption has not b作者: watertight, 時間: 2025-3-24 00:44
Nasal Potential Difference Measurements to Assess CFTR Ion Channel Activityt and reflects in part CFTR function. The electrophysiologic abnormality in cystic fibrosis was first described 30 years ago and correlates with features of the CF phenotype. NPD measurement is an important in vivo research and diagnostic tool, and is used to assess the efficacy of new treatments su作者: 開花期女 時間: 2025-3-24 05:49
Measurement of Ion Transport Function in Rectal Biopsies an anion channel and is known to interact with a number of other cellular proteins involved in ion transport. To date more than 1,800 mutations are known, most of which result in various degrees of impaired transport function of the gene product. Due to the high inter-individual variability of dise作者: CREEK 時間: 2025-3-24 09:57 作者: Circumscribe 時間: 2025-3-24 12:45
Quantification of CFTR Transcriptsting the efficiency of various therapeutic approaches to CF, including gene therapy. Reverse transcription (RT) followed by quantitative polymerase chain reaction (qPCR) is at present the most sensitive method for transcript abundance measurement. Classical RNA-based methods require significant expr作者: 恩惠 時間: 2025-3-24 15:12 作者: Foolproof 時間: 2025-3-24 20:16
Approaches to Study CFTR Pre-mRNA Splicing Defects evaluation of their pathological effect is an important aspect both in the diagnostic field and in the study of basic regulatory mechanism. Efficient and correct splicing of CFTR relies on a complex process that includes recognition within the nascent transcripts of a series of different splicing r作者: 愉快么 時間: 2025-3-25 02:25 作者: 酷熱 時間: 2025-3-25 05:02 作者: mediocrity 時間: 2025-3-25 10:51 作者: LINE 時間: 2025-3-25 12:10
Analysis of CFTR Folding and Degradation in Transiently Transfected Cellsthe surface of the endoplasmic reticulum to select misfolded proteins for degradation. The folding status of F508del-CFTR is monitored by at least two ER quality control checkpoints. The ER-associated Derlin-1/RMA1 E3 complex appears to recognize folding defects in CFTR that involve misassembly of N作者: GLUE 時間: 2025-3-25 16:35 作者: BIBLE 時間: 2025-3-25 20:41
B. Olivier,J. Mos,J. L. Slangenhe development and validation of the CF HBE pharmacology model and its use to characterize, optimize, and select clinical candidates. It is expected that the pre-clinical testing of CFTR potentiators and correctors using epithelial cell cultures derived from CF patients will help to increase their likelihood of clinical efficacy.作者: 灰姑娘 時間: 2025-3-26 00:42
Animal Models in Psychopharmacologyy available delivery method and established and novel outcome measures. We highlight the logistic and regulatory complexities of such a clinical programme and finally, we look to the future and consider possible alternative strategies.作者: ostrish 時間: 2025-3-26 04:31
https://doi.org/10.1007/978-3-031-46456-0f digested and ligated, crosslinked chromosomes to measure physical interactions between distal genomic regions. When used together, these methods provide a powerful avenue to discover transcriptional regulatory elements within large genomic regions.作者: 良心 時間: 2025-3-26 12:19 作者: Immunization 時間: 2025-3-26 14:03
Use of Primary Cultures of Human Bronchial Epithelial Cells Isolated from Cystic Fibrosis Patients fhe development and validation of the CF HBE pharmacology model and its use to characterize, optimize, and select clinical candidates. It is expected that the pre-clinical testing of CFTR potentiators and correctors using epithelial cell cultures derived from CF patients will help to increase their likelihood of clinical efficacy.作者: Nebulous 時間: 2025-3-26 18:34
Design of Gene Therapy Trials in CF Patientsy available delivery method and established and novel outcome measures. We highlight the logistic and regulatory complexities of such a clinical programme and finally, we look to the future and consider possible alternative strategies.作者: occult 時間: 2025-3-27 00:42
Genomic Approaches to Studying , Transcriptional Regulationf digested and ligated, crosslinked chromosomes to measure physical interactions between distal genomic regions. When used together, these methods provide a powerful avenue to discover transcriptional regulatory elements within large genomic regions.作者: indicate 時間: 2025-3-27 01:28
Analysis of CFTR Folding and Degradation in Transiently Transfected Cellsadation. The experimental system described employs transiently transfected HEK293 cells and is utilized to monitor the biogenesis of CFTR by both Western blot and pulse chase studies. Methods to detect complexes formed between CFTR folding intermediates and ER quality control factors will also be described.作者: Cubicle 時間: 2025-3-27 05:48 作者: 諂媚于性 時間: 2025-3-27 12:16 作者: 窩轉(zhuǎn)脊椎動物 時間: 2025-3-27 13:43 作者: Prostaglandins 時間: 2025-3-27 17:53 作者: 小丑 時間: 2025-3-28 01:59 作者: 騷動 時間: 2025-3-28 02:08 作者: 借喻 時間: 2025-3-28 08:37 作者: Jubilation 時間: 2025-3-28 10:45 作者: 把手 時間: 2025-3-28 17:23
Parasitic Protozoa in Macrophages in vitroome correctors appear to function as pharmacological chaperones that specifically bind to the CFTR processing mutants and induce them to complete the folding process. In this chapter, we describe techniques to examine the effects of correctors on folding of CFTR processing mutants.作者: 向下 時間: 2025-3-28 19:56
Da-Zhi Liu,Bradley P. Ander,Frank R. Sharper concentrates on methods for evaluating microRNA regulation of gene expression in the context of airway disease. The final chapter considers methods to evaluate the chromatin structure of the active . locus and to analyse the .-acting regulatory elements that control it作者: Agility 時間: 2025-3-29 01:11 作者: 合乎習俗 時間: 2025-3-29 06:51 作者: 嗎啡 時間: 2025-3-29 10:44
Urveshkumar D. Patel,M. R. Srinivasanres of the CF phenotype. NPD measurement is an important in vivo research and diagnostic tool, and is used to assess the efficacy of new treatments such as gene therapy and ion transport modulators. This chapter will elaborate on the electrophysiological principles behind the test, the equipment required, the methods, and the analysis of the data.作者: 滋養(yǎng) 時間: 2025-3-29 13:31 作者: 乞丐 時間: 2025-3-29 16:40 作者: meritorious 時間: 2025-3-29 21:07
High-Throughput Screening of Libraries of Compounds to Identify CFTR Modulatorsthe halide sensitivity of the yellow fluorescent protein (YFP). CFTR activity can be simply assessed by measuring the rate of YFP signal decrease caused by iodide influx. This assay can be automated to test thousands of compounds per day.作者: 斜谷 時間: 2025-3-30 02:10
Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperonesome correctors appear to function as pharmacological chaperones that specifically bind to the CFTR processing mutants and induce them to complete the folding process. In this chapter, we describe techniques to examine the effects of correctors on folding of CFTR processing mutants.作者: 能量守恒 時間: 2025-3-30 06:42 作者: 大包裹 時間: 2025-3-30 08:51 作者: Brain-Imaging 時間: 2025-3-30 15:41 作者: 專橫 時間: 2025-3-30 19:50
Bridget M. Ogilvie,Michele Jungeryls to confirm the diagnosis of CF in subjects with suggestive symptoms. Since these tests reflect CFTR function and thus relate to the basic disease process in CF, changes in these parameters are also being used to assess the pharmacologic effect of compounds aimed at restoring CFTR function. Howeve作者: 毛細血管 時間: 2025-3-31 00:14
https://doi.org/10.1007/978-1-349-06136-5various pathological conditions including cystic fibrosis and secretory diarrhea. The identification of CFTR pharmacological modulators may be achieved by screening highly diverse synthetic or natural compound libraries using high-throughput methods. A convenient assay for CFTR function is based on 作者: Decongestant 時間: 2025-3-31 01:26
Parasitic Protozoa in Macrophages in vitrof the protein and trafficking to the cell surface. Processing mutations appear to inhibit folding of CFTR so that it accumulates in the endoplasmic reticulum as a partially folded protein. Expressing the proteins in the presence of small molecules called correctors can repair CFTR folding defects. S作者: 遺產(chǎn) 時間: 2025-3-31 09:00
B. Olivier,J. Mos,J. L. Slangencorrectors and potentiators on CFTR function and epithelial cell biology in the native pathological environment. Cultured HBE cells derived from CF patients exhibit many of the morphological and functional characteristics believed to be associated with CF airway disease in vivo, including abnormal i
