標(biāo)題: Titlebook: Cryptosporidium; Methods and Protocol Jan R. Mead,Michael J. Arrowood Book 2020 Springer Science+Business Media, LLC, part of Springer Natu [打印本頁(yè)] 作者: minutia 時(shí)間: 2025-3-21 18:53
書(shū)目名稱Cryptosporidium影響因子(影響力)
作者: GROSS 時(shí)間: 2025-3-21 23:27 作者: 織布機(jī) 時(shí)間: 2025-3-22 01:11
A Practical Guide to Study Design,d in community-wide or nation-wide serosurveys. Here we describe the coupling of the . recombinant antigens to carboxylated polystyrene beads, the data acquisition and analysis of IgG antibodies bound to the coupled beads, and the quality control methods required for data validation using the Lumine作者: keloid 時(shí)間: 2025-3-22 05:33
Sampling Strategies: Fundamentals,ng alveolate outgroups of the Apicomplexa, . and .. Recent years have seen several new genome sequences for both existing and new . species as well as transcriptomics, proteomics, SNP, and isolate population surveys. This chapter introduces the extensive data mining and visualization capabilities of作者: allude 時(shí)間: 2025-3-22 11:25
A Practical Guide to Study Design, methodology provides an effective strategy to study the role of selected genes in host–parasite interactions, and also can be used to identify potential targets for chemotherapy. The standardized methodology based on this strategy is described in this chapter.作者: hair-bulb 時(shí)間: 2025-3-22 13:13 作者: hair-bulb 時(shí)間: 2025-3-22 19:00
Measuring , Serologic Responses by Multiplex Bead Assay,d in community-wide or nation-wide serosurveys. Here we describe the coupling of the . recombinant antigens to carboxylated polystyrene beads, the data acquisition and analysis of IgG antibodies bound to the coupled beads, and the quality control methods required for data validation using the Lumine作者: 整頓 時(shí)間: 2025-3-23 00:19 作者: 阻礙 時(shí)間: 2025-3-23 05:27 作者: 雄辯 時(shí)間: 2025-3-23 09:17 作者: TOXIC 時(shí)間: 2025-3-23 11:27
Sampling Strategies: Applications,development and commercialization of improved enzyme immunosorbent assays (EIA) for coproantigen detection provided an automatable method for mass testing, and rapid diagnostics when incorporated onto a cartridge format. Similarly, immunochromatographic lateral flow assays (ICLF) enable rapid diagno作者: 使隔離 時(shí)間: 2025-3-23 13:58 作者: OATH 時(shí)間: 2025-3-23 20:17
A Practical Guide to Study Design,nd ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to . isolation or nucleic acid extraction methods for the detection of . oocysts or . DNA.作者: 審問(wèn) 時(shí)間: 2025-3-24 01:30 作者: Entirety 時(shí)間: 2025-3-24 05:30 作者: Projection 時(shí)間: 2025-3-24 10:28
Sampling Strategies: Applications,s surface and apical complex antigens are posttranslationally modified with O- and N-linked glycans. This presents a significant challenge to understanding the functions of these antigens and the immune responses to them. Isolation of large amounts of native antigen from . oocysts is expensive and i作者: 貧困 時(shí)間: 2025-3-24 12:59
Sampling Strategies: Fundamentals,wn utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for . genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequen作者: slipped-disk 時(shí)間: 2025-3-24 17:06 作者: Thyroiditis 時(shí)間: 2025-3-24 21:09
Inventory and Monitoring Studies,able culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality . DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.作者: 門(mén)閂 時(shí)間: 2025-3-25 02:31 作者: Thymus 時(shí)間: 2025-3-25 03:56 作者: Indict 時(shí)間: 2025-3-25 11:06 作者: Ligneous 時(shí)間: 2025-3-25 13:16
Fragrance Materials in Wastewater Treatment,ure this diarrhea-inducing enteric disease caused by . spp. protozoan parasites. There is an essential need to discover new therapeutic drugs efficient in reducing parasite burden in infected individuals. Research therefore relies on reliable small animal models of cryptosporidiosis. Here, we presen作者: 改正 時(shí)間: 2025-3-25 16:38 作者: Fibroid 時(shí)間: 2025-3-25 22:16 作者: moratorium 時(shí)間: 2025-3-26 01:51 作者: 挫敗 時(shí)間: 2025-3-26 05:59 作者: ectropion 時(shí)間: 2025-3-26 11:19 作者: 激怒 時(shí)間: 2025-3-26 16:04 作者: LINE 時(shí)間: 2025-3-26 17:14 作者: 高原 時(shí)間: 2025-3-26 22:52 作者: deviate 時(shí)間: 2025-3-27 01:25 作者: 寬容 時(shí)間: 2025-3-27 09:13
Preparation of , DNA for Whole Genome Sequencing,able culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality . DNA for WGS from feces, incorporating parasite purification stages and an optional whole genome amplification step.作者: Medicaid 時(shí)間: 2025-3-27 10:30
High-Throughput Screening of Drugs Against the Growth of , In Vitro by qRT-PCR,ut screening (HTS) of drugs against . in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of . in vitro.作者: 燈絲 時(shí)間: 2025-3-27 14:30 作者: malign 時(shí)間: 2025-3-27 18:53 作者: aggrieve 時(shí)間: 2025-3-28 00:14 作者: –scent 時(shí)間: 2025-3-28 02:47 作者: SEED 時(shí)間: 2025-3-28 06:46
Cryptosporidium978-1-4939-9748-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: WITH 時(shí)間: 2025-3-28 12:14
Inventory and Monitoring Studies, half have been reported from humans, and most human cases of cryptosporidiosis are caused by . or .. Here we describe a nested PCR for the detection of all . spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of . and ..作者: IRATE 時(shí)間: 2025-3-28 16:16
A Practical Guide to Study Design,nd ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to . isolation or nucleic acid extraction methods for the detection of . oocysts or . DNA.作者: Keratin 時(shí)間: 2025-3-28 20:00 作者: Dungeon 時(shí)間: 2025-3-29 01:51 作者: 過(guò)于光澤 時(shí)間: 2025-3-29 07:03
Enterprise Business Integration in 2010A.D.ut screening (HTS) of drugs against . in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of . in vitro.作者: Stagger 時(shí)間: 2025-3-29 10:36
https://doi.org/10.1007/978-1-4939-9748-0Protozoan; Intestinal tract infection; Anti-cryptosporidial drugs; Genotyping; Oocysts; CRISPR/Cas9; Host-作者: 不發(fā)音 時(shí)間: 2025-3-29 15:04 作者: 大方一點(diǎn) 時(shí)間: 2025-3-29 18:04
Fragrance Materials in Wastewater Treatment,from stool and intestine of infected mice to facilitate oocyst quantification. Moreover, we present protocols using flow cytometry, quantitative polymerase chain reaction, and histopathology to accurately quantify parasite burden in stool or intestine samples.作者: machination 時(shí)間: 2025-3-29 21:23
Vladimir Tosic,Kruti Patel,Bernard Pagurekssay readout. Here we describe an established 384- or 1536-well format high-content imaging (HCI) assay of .-infected HCT-8 human ileocecal adenocarcinoma cells. This HCS assay is a powerful tool to assess large numbers of compounds to power drug discovery, as well as to phenotypically characterize known .-active compounds.作者: arboretum 時(shí)間: 2025-3-30 03:27
Mouse Models for Use in , Infection Studies and Quantification of Parasite Burden Using Flow Cytomefrom stool and intestine of infected mice to facilitate oocyst quantification. Moreover, we present protocols using flow cytometry, quantitative polymerase chain reaction, and histopathology to accurately quantify parasite burden in stool or intestine samples.作者: 新手 時(shí)間: 2025-3-30 05:31 作者: STRIA 時(shí)間: 2025-3-30 12:08 作者: 凹室 時(shí)間: 2025-3-30 13:08
In Vitro Culture of , Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic vitro growth of . that produces sexual and asexual stages. The method enables the evaluation of potential therapeutic compounds under conditions that mirror the dynamic conditions found in the gut facilitating preliminary pharmacokinetic and pharmacodynamic data to be obtained.作者: 易彎曲 時(shí)間: 2025-3-30 18:22
Sampling Strategies: Applications,stics. Nevertheless, it is important that positive reactions by EIA or ICLF are confirmed. Here we describe microscopical methods using tinctorial stains for the diagnosis of acute cryptosporidiosis, and using immunofluorescent reagents for diagnosis or for confirmation of EIA or ICLF positive reactions.作者: 無(wú)動(dòng)于衷 時(shí)間: 2025-3-31 00:17
Sampling Strategies: Applications,s only feasible for . antigens. Here, we describe a method of producing recombinant, functional . glycoprotein antigens in .. These functional recombinant proteins can be used to investigate the role of glycotopes in . immune responses and parasite–host cell interactions.作者: 管理員 時(shí)間: 2025-3-31 04:43
Inventory and Monitoring Studies,gene, and glycoprotein 60 are amplified using conventional PCR. Amplified and sequenced genes are compared to a reference library of up-to-date curated gene sequences to identify . species and variants.作者: 蒙太奇 時(shí)間: 2025-3-31 05:52
Book 2020 as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for .in vitro. cell culture techniques to address the issues with growing this difficult organism continuously. Written for the hig作者: 誘拐 時(shí)間: 2025-3-31 09:46
1064-3745 expertsThis book encompasses broad aspects of .Cryptosporidium. research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics,作者: 澄清 時(shí)間: 2025-3-31 15:17
The Handbook of Environmental Chemistry/or microbial offense during host–parasite interactions. Here, several methodologies to explore the role for miRNAs in host–parasite interactions are briefly summarized, including the detection, quantification, and intracellular localization of miRNAs, identification and validation of miRNA targets, and functional manipulation of specific miRNAs.作者: 可能性 時(shí)間: 2025-3-31 21:03
Akhil Kumar,Jacques Wainer,Zuopeng Zhangvitro growth of . that produces sexual and asexual stages. The method enables the evaluation of potential therapeutic compounds under conditions that mirror the dynamic conditions found in the gut facilitating preliminary pharmacokinetic and pharmacodynamic data to be obtained.作者: Licentious 時(shí)間: 2025-3-31 23:36
Sampling Strategies: Fundamentals,sts purified as described in this chapter are suitable for animal infection studies, cell culture studies, and a wide range of molecular biological studies, environmental studies, drug testing, and disinfection studies.
