標(biāo)題: Titlebook: Cryptococcus neoformans; Methods and Protocol Erin E. McClelland Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁(yè)] 作者: 連結(jié) 時(shí)間: 2025-3-21 19:01
書(shū)目名稱Cryptococcus neoformans影響因子(影響力)
作者: 使隔離 時(shí)間: 2025-3-21 22:56
Hermeneutics, Social Theory and Educationg levels in the CSF, profile the immune response in the CSF, and/or characterize the yeast cells. The rabbit model of infection is a robust experimental model for better understanding CM and . cellular behavior.作者: hypotension 時(shí)間: 2025-3-22 04:00 作者: SEVER 時(shí)間: 2025-3-22 04:49
The Rabbit Model of Cryptococcal Meningitisg levels in the CSF, profile the immune response in the CSF, and/or characterize the yeast cells. The rabbit model of infection is a robust experimental model for better understanding CM and . cellular behavior.作者: 指派 時(shí)間: 2025-3-22 11:52 作者: Restenosis 時(shí)間: 2025-3-22 14:04 作者: Restenosis 時(shí)間: 2025-3-22 19:09
978-1-0716-3724-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: fiction 時(shí)間: 2025-3-22 23:26
A , Model of , Infectionpliers and infected with .. Injection into the proleg of larvae results in systemic infections. Larvae may then be monitored for survival or homogenized to determine fungal burden. Fixation of infected larvae produces samples suitable for histological staining and analysis.作者: 隼鷹 時(shí)間: 2025-3-23 01:24
Transcriptome Analysis to Identify Differentially Expressed Genes Using the STAR Pipeline in CyVerss of the data is typically a multistep process using several bioinformatics tools. The following protocol utilizes a reliable pipeline for identifying differentially expressed genes among samples of . that is approachable for the adventurous beginner.作者: escalate 時(shí)間: 2025-3-23 06:10 作者: ANTE 時(shí)間: 2025-3-23 12:26
Three Theories of Knowledge in Educationpliers and infected with .. Injection into the proleg of larvae results in systemic infections. Larvae may then be monitored for survival or homogenized to determine fungal burden. Fixation of infected larvae produces samples suitable for histological staining and analysis.作者: Dorsal-Kyphosis 時(shí)間: 2025-3-23 16:38
Hermeneutics, Social Theory and Educationnter the body through the lungs and then escape due to dysregulation of the immune response. Multiple animal species have been used to model the infection and characterize CM including mice, rats, dogs, guinea pigs, and rabbits. The rabbit model has over 40?years of data and has been used to study h作者: 重疊 時(shí)間: 2025-3-23 18:25 作者: compose 時(shí)間: 2025-3-24 00:57 作者: Mortal 時(shí)間: 2025-3-24 02:46
Tracking Study on FDP Implementation,oated gold beads by a helium shock wave produced using a biolistic particle system. The procedure often involves insertion of a dominant selectable marker into the desired site by homologous recombination. To increase the likelihood of homologous recombination, large fragments of overlapping DNA are作者: 尊重 時(shí)間: 2025-3-24 10:35
Reservoir Development Principle, has been used for the stable introduction of exogenous DNA into . for over two decades, being particularly impactful for insertional mutagenesis screens to discover new genes involved in fungal biology. A detailed protocol to conduct this transformation method is provided in the chapter. Scope for 作者: 駕駛 時(shí)間: 2025-3-24 12:29
Adjustment to Development Strategy,silencing effect occurs because of decreased levels of mRNA that then result in decreased protein levels for the gene. The specificity of the silencing is dependent upon the presence of sequence-specific double-stranded RNA (dsRNA) that activates the cellular RNAi machinery. This chapter describes t作者: 過(guò)分 時(shí)間: 2025-3-24 16:04 作者: 向前變橢圓 時(shí)間: 2025-3-24 19:58 作者: myriad 時(shí)間: 2025-3-25 00:19 作者: Defiance 時(shí)間: 2025-3-25 07:17 作者: 制造 時(shí)間: 2025-3-25 09:03 作者: municipality 時(shí)間: 2025-3-25 14:17
Yanfeng Zhang,Qiange Wang,Shufeng Gongand life-threatening infections of the central nervous system. Following inhalation of yeasts or desiccated basidiospores into the lung alveoli, resident pulmonary phagocytic cells aid in the identification and eradication of . yeast through their arsenal of pattern recognition receptors (PRRs). PRR作者: deciduous 時(shí)間: 2025-3-25 16:01
Chunming Yang,Xinghao Song,Hui Zhang,Bo Li such, measuring fungal burden of . strains—and indeed how drug treatments can influence fungal burden—provides important information about . pathogenesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating作者: Free-Radical 時(shí)間: 2025-3-25 23:24
Erin E. McClellandIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: PANG 時(shí)間: 2025-3-26 00:36 作者: 征稅 時(shí)間: 2025-3-26 07:12
Cryptococcus neoformans978-1-0716-3722-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-26 08:44 作者: 會(huì)犯錯(cuò)誤 時(shí)間: 2025-3-26 14:42
https://doi.org/10.1007/978-981-16-0479-9s of the data is typically a multistep process using several bioinformatics tools. The following protocol utilizes a reliable pipeline for identifying differentially expressed genes among samples of . that is approachable for the adventurous beginner.作者: 性行為放縱者 時(shí)間: 2025-3-26 17:50 作者: 向下五度才偏 時(shí)間: 2025-3-27 00:31 作者: gratify 時(shí)間: 2025-3-27 01:18 作者: Collected 時(shí)間: 2025-3-27 06:54 作者: 沙文主義 時(shí)間: 2025-3-27 10:10 作者: 安撫 時(shí)間: 2025-3-27 13:41
Biolistic Transformation of oated gold beads by a helium shock wave produced using a biolistic particle system. The procedure often involves insertion of a dominant selectable marker into the desired site by homologous recombination. To increase the likelihood of homologous recombination, large fragments of overlapping DNA are作者: Incumbent 時(shí)間: 2025-3-27 19:36 作者: Mammal 時(shí)間: 2025-3-28 01:19 作者: FOR 時(shí)間: 2025-3-28 02:41 作者: comely 時(shí)間: 2025-3-28 10:08 作者: correspondent 時(shí)間: 2025-3-28 13:59 作者: Ischemic-Stroke 時(shí)間: 2025-3-28 14:39 作者: acrophobia 時(shí)間: 2025-3-28 22:06
Interaction Between Macrophages and ,: Distinguishing Phagocytosed Versus External Fungia niche in which facultative intracellular parasites, such as ., thrive. Consequently, phagocytosis of cryptococcal cells and its outcomes are very frequently studied. One major issue with several of the tests used for this, however, is that macrophage–. interaction does not always result in phagocy作者: CLAM 時(shí)間: 2025-3-29 02:02
Detection and Quantification of , Uptake by Phagocytic Cells Using Imaging Flow Cytometryand life-threatening infections of the central nervous system. Following inhalation of yeasts or desiccated basidiospores into the lung alveoli, resident pulmonary phagocytic cells aid in the identification and eradication of . yeast through their arsenal of pattern recognition receptors (PRRs). PRR作者: 泥瓦匠 時(shí)間: 2025-3-29 06:54
Two Methods of Measuring , Fungal Burden in Macrophages such, measuring fungal burden of . strains—and indeed how drug treatments can influence fungal burden—provides important information about . pathogenesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating作者: 芳香一點(diǎn) 時(shí)間: 2025-3-29 07:47 作者: 割公牛膨脹 時(shí)間: 2025-3-29 12:22
Two Methods of Measuring , Fungal Burden in Macrophagesesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating the average number of yeast per host cell and a fluorescence microscopy-based method that may be used to measure changes in fungal burden in individual living macrophages in real time.作者: Infuriate 時(shí)間: 2025-3-29 18:23 作者: 打折 時(shí)間: 2025-3-29 22:33
Xiaohuan Shan,Xiyi Shi,Wenyuan Ma,Junlu Wangof macrophages, or yeast killing. Differentiating internalized versus external . cells is thus essential to evaluate monocyte–macrophage phagocytosis. We describe here a protocol that allows quantification of . spp. phagocytosis using quantitative flow cytometry in human monocytes and a murine macrophage cell line (J774).作者: HPA533 時(shí)間: 2025-3-30 00:46
1064-3745 ation advice from the experts.This volume explores the latest developments in the study of .Cryptococcus neoformans .and its pathology, along with discussion on newly used therapeutics. The chapters in this book cover a wide range of protocols commonly used in .Cryptococcus. research such as animal 作者: offense 時(shí)間: 2025-3-30 06:09
Reservoir Development Principle,ens to discover new genes involved in fungal biology. A detailed protocol to conduct this transformation method is provided in the chapter. Scope for modifications and the benefits and disadvantages of using .MT in . species are also presented.作者: 拱形面包 時(shí)間: 2025-3-30 10:40
Chunming Yang,Xinghao Song,Hui Zhang,Bo Liesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating the average number of yeast per host cell and a fluorescence microscopy-based method that may be used to measure changes in fungal burden in individual living macrophages in real time.作者: 招惹 時(shí)間: 2025-3-30 13:56
Hermeneutics, Social Theory and Education three commonly used strategies to establish cryptococcosis in mice: intranasal, intratracheal, and intravenous inoculations. Also, we discuss the methodology for delivering drugs to mice via intraperitoneal injection.作者: 殖民地 時(shí)間: 2025-3-30 20:27 作者: 自制 時(shí)間: 2025-3-31 00:28
Chunming Yang,Xinghao Song,Hui Zhang,Bo Liibe sample preparation, followed by purification, processing on the mass spectrometer, and a robust bioinformatics analysis. The information gleaned from proteomic profiling of fungal infections supports the detection of novel biomarkers for diagnostic and prognostic purposes.作者: Ejaculate 時(shí)間: 2025-3-31 04:45
