標(biāo)題: Titlebook: Cryotechniques in Biological Electron Microscopy; Rudolf Alexander Steinbrecht,Karl Zierold Book 1987 Springer-Verlag Berlin Heidelberg 19 [打印本頁] 作者: Lactase 時間: 2025-3-21 19:56
書目名稱Cryotechniques in Biological Electron Microscopy影響因子(影響力)
書目名稱Cryotechniques in Biological Electron Microscopy影響因子(影響力)學(xué)科排名
書目名稱Cryotechniques in Biological Electron Microscopy網(wǎng)絡(luò)公開度
書目名稱Cryotechniques in Biological Electron Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cryotechniques in Biological Electron Microscopy被引頻次
書目名稱Cryotechniques in Biological Electron Microscopy被引頻次學(xué)科排名
書目名稱Cryotechniques in Biological Electron Microscopy年度引用
書目名稱Cryotechniques in Biological Electron Microscopy年度引用學(xué)科排名
書目名稱Cryotechniques in Biological Electron Microscopy讀者反饋
書目名稱Cryotechniques in Biological Electron Microscopy讀者反饋學(xué)科排名
作者: sterilization 時間: 2025-3-21 20:28 作者: 積云 時間: 2025-3-22 03:46 作者: Isolate 時間: 2025-3-22 07:50 作者: 昏暗 時間: 2025-3-22 09:43 作者: ineptitude 時間: 2025-3-22 13:20 作者: ineptitude 時間: 2025-3-22 19:43
Wenxing Zhong,Dehong Xu,Ron Shu Yuen Huitivated process, meaning that the molecules involved need excess energy (activation energy) in order to be able to change their positions. This “activation energy” is released after the event has taken place. Molecules which are not at the activated energy level will not react, although the reaction作者: abysmal 時間: 2025-3-23 00:07
Basic Theory of Magnetic Resonance WPT segregation compartments formed by the growing ice crystals within the specimen increases so rapidly that deeper layers cannot be used for electron microscopy. The depth of the well-preserved border zone can be increased without chemical pretreatment to, at the most, 300 μm by applying high pressur作者: 去世 時間: 2025-3-23 03:49
K. R. Venugopal,Sejal Santosh Nimbhorkarical electron microscopy, for successful FS and FD the main prerequisite is also good cryofixation with as little freezing damage as possible (see Bachmann and Mayer, Chap. 1; Sitte et al., Chap. 4; Dubochet et al., Chap. 5; Moor, Chap. 8; this Vol.).作者: 吼叫 時間: 2025-3-23 05:51
K. R. Venugopal,K. C. Srikantaiah namely they only enable satisfactory cryofixation of objects or superficial layers, which are not thicker than 10–20 μm. This limitation is caused by the physical properties of aqueous systems and it indicates that thicker specimens can be well cryofixed only if these properties are altered.作者: 不能根除 時間: 2025-3-23 10:05
Margaret Kovach,Bonnie Stelmachs and Lindeman 1975; Brom-bach 1975; Lechene et al. 1975; Marshall 1975a,b; Forrest and Marshall 1976; Zierold 1976; Marshall 1977; Yeo et al. 1977; Fuchs et al. 1978a,b; Kramer and Preston 1978; Zierold and Sch?fer 1978; Echlin et al 1980; Fuchs and Fuchs 1980; Marshall 1980a,b; Echlin et al. 1981;作者: fibula 時間: 2025-3-23 16:34 作者: 縮影 時間: 2025-3-23 21:14 作者: 欺騙手段 時間: 2025-3-23 23:00 作者: Acumen 時間: 2025-3-24 04:00 作者: Working-Memory 時間: 2025-3-24 09:39
Wenxing Zhong,Dehong Xu,Ron Shu Yuen Huin of ice crystals (see Bachmann and Mayer, Chap. 1, this Vol.). Due to their size, they disturb the fine structure of cells (see also Sitte et al., Chap. 4, this Vol.; Moor, Chap. 8, this Vol.). In the present chapter, we consider the problems which we face when we are able to work in vitreous ice, 作者: 感染 時間: 2025-3-24 11:40 作者: exigent 時間: 2025-3-24 15:10 作者: RODE 時間: 2025-3-24 19:44 作者: 直覺沒有 時間: 2025-3-24 23:45 作者: fiction 時間: 2025-3-25 05:19 作者: Gnrh670 時間: 2025-3-25 11:01 作者: 我悲傷 時間: 2025-3-25 15:14
Hiroko Kubota,Muna Saleh,Jinny Menon%. In previous chapters of this volume, it has been discussed that cryofixation of almost pure water without ice formation and phase separation is a most difficult task. This explains why all attempts to make interactions of colloidal particles (“network formation”) visible by cryotechniques have ut作者: 古董 時間: 2025-3-25 18:15
Gendered Inequities in the Academyspecimens (Nermut and Frank 1971; Kistler et al. 1977; Gross et al. 1985). Very thin specimens such as isolated membranes or isolated macromolecules can be directly adsorbed on carbon-coated grids. After rapid freezing, the grids are transferred into suitable high-vacuum equipment for freeze-drying 作者: 忘恩負(fù)義的人 時間: 2025-3-25 20:50 作者: 否決 時間: 2025-3-26 03:48
Hiroko Kubota,Muna Saleh,Jinny Menoncytochemistry, the classical methods in histochemistry and cytochemistry. It is an unpleasant task to summarize the present situation and to arrive at the conclusion that these promises are only partly fulfilled. For the autoradiography of diffusible substances, there seem to be only practical limit作者: 火光在搖曳 時間: 2025-3-26 05:41 作者: CLAP 時間: 2025-3-26 10:06
Gendered Inequities in the Academyponents (Plattner and Bachmann 1982). This intention of fixation is, of course, not restricted to applications in electron microscopy. Attempts to literally “freeze” the living structure by cryofixation can be traced back to the last century, and were extended to electron microscopy in its very earl作者: fabricate 時間: 2025-3-26 15:20
Margaret Kovach,Bonnie Stelmachprinciple, the irradiating electron beam (probe) is focussed on the area of interest in the specimen, while the secondary radiation (e.g. X-rays) generated by the interaction with the specimen is processed through a spectrometer to provide information on the kind and amount of the elements present. 作者: 虛情假意 時間: 2025-3-26 18:20 作者: 路標(biāo) 時間: 2025-3-26 20:57
https://doi.org/10.1007/978-3-642-72815-0X-ray; electron microscopy; enzyme; enzymes; high pressure; microscopy; molecule; replication; tissue作者: 溺愛 時間: 2025-3-27 02:58
978-3-642-72817-4Springer-Verlag Berlin Heidelberg 1987作者: Rinne-Test 時間: 2025-3-27 07:42
Physics of Water and Ice: Implications for Cryofixationsts under physiological conditions. Ideal fixation and specimen preparation require that the constituents of the system keep their position within a range which is smaller than the resolution of the observation technique. The molecules and ions within a biological specimen interact in very complex w作者: 廣口瓶 時間: 2025-3-27 12:55 作者: 顯微鏡 時間: 2025-3-27 15:01 作者: Congregate 時間: 2025-3-27 19:23
Cryofixation Without Pretreatment at Ambient Pressurend to better maintain their chemical constitution (Altmann 1890). This alternative to chemical fixation gained in importance during the first half of this century due to the availability of liquefied air used as coolant (“cryogen”) as well as to the fact that effective cooling systems for cryostats 作者: tympanometry 時間: 2025-3-27 23:35 作者: alabaster 時間: 2025-3-28 05:29
Cryoultramicrotomyomposition representing as close as possible the natural state. Cryosections offer the unique possibility to study cells unaffected by chemical treatment, staining and embedding. The present state of the art of cryoultramicrotomy can fulfill this aim only partly: the section thickness achieved routi作者: FECK 時間: 2025-3-28 09:24 作者: Budget 時間: 2025-3-28 11:24 作者: Geyser 時間: 2025-3-28 15:07 作者: Pruritus 時間: 2025-3-28 20:05
High Resolution Metal Replication of Freeze-Dried Specimensspecimens (Nermut and Frank 1971; Kistler et al. 1977; Gross et al. 1985). Very thin specimens such as isolated membranes or isolated macromolecules can be directly adsorbed on carbon-coated grids. After rapid freezing, the grids are transferred into suitable high-vacuum equipment for freeze-drying 作者: 門閂 時間: 2025-3-29 01:09 作者: 過分自信 時間: 2025-3-29 05:29
Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistrycytochemistry, the classical methods in histochemistry and cytochemistry. It is an unpleasant task to summarize the present situation and to arrive at the conclusion that these promises are only partly fulfilled. For the autoradiography of diffusible substances, there seem to be only practical limit作者: expository 時間: 2025-3-29 10:42
Scanning Electron Microscopy and X-Ray Microanalysis of Frozen-Hydrated Bulk Samplesntation of the living state that is currently possible to obtain in the electron microscope. An exception is the “environmental microscope” (Danilatos and Postle 1982), but this has considerably more limitations in its use than the “l(fā)ow temperature microscope”. The methods and techniques of low temp作者: 極肥胖 時間: 2025-3-29 12:44
Cryofixation of Dynamic Processes in Cells and Organellesponents (Plattner and Bachmann 1982). This intention of fixation is, of course, not restricted to applications in electron microscopy. Attempts to literally “freeze” the living structure by cryofixation can be traced back to the last century, and were extended to electron microscopy in its very earl作者: Neutral-Spine 時間: 2025-3-29 16:50
Cryofixation of Diffusible Elements in Cells and Tissues for Electron Probe Microanalysisprinciple, the irradiating electron beam (probe) is focussed on the area of interest in the specimen, while the secondary radiation (e.g. X-rays) generated by the interaction with the specimen is processed through a spectrometer to provide information on the kind and amount of the elements present. 作者: capsaicin 時間: 2025-3-29 21:25
Wenxing Zhong,Dehong Xu,Ron Shu Yuen Huihieved with procedures that allow for averaging from identical subunits. At the other level, we consider rearrangements and small aggregations of macromolecules due to the specimen preparation. This is the resolution of 10–20 nm achieved normally with the current methods of preparation of biological material.作者: 通便 時間: 2025-3-30 01:10 作者: wall-stress 時間: 2025-3-30 07:23 作者: JOT 時間: 2025-3-30 09:41
The Response of Biological Macromolecules and Supramolecular Structures to the Physics of Specimen Chieved with procedures that allow for averaging from identical subunits. At the other level, we consider rearrangements and small aggregations of macromolecules due to the specimen preparation. This is the resolution of 10–20 nm achieved normally with the current methods of preparation of biological material.作者: 完全 時間: 2025-3-30 13:04
Cryoelectron Microscopy of Vitrified Specimensthe last 20 years were not aimed at preserving the hydration of the specimen in the electron microscope. Freezing was only used as an aid to preparation. The objects ultimately observed in the electron microscope were dry and at room temperature. Such cryomethods have recently been reviewed in detail (Robards and Sleytr 1985).作者: 羊欄 時間: 2025-3-30 18:03
Cryofixation of Dynamic Processes in Cells and Organellesmical fixation procedures have routinely been used, although their limitations and risks have been well recognized (Fernandez-Moran 1964; Rebhun 1965). On the other hand, the drawbacks of chemical treatments have stimulated the development of rapid freezing techniques which reduce the formation of ice crystals (Van Harreveld and Crowell 1964).
