標題: Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harri?tte Oldenhof Book 2015Latest edition Springer Science+Business Media [打印本頁] 作者: squamous-cell 時間: 2025-3-21 18:08
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書目名稱Cryopreservation and Freeze-Drying Protocols讀者反饋學科排名
作者: lactic 時間: 2025-3-21 20:38
Timothy Kerswell,Surendra Pratap the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.作者: ELUDE 時間: 2025-3-22 01:56
on a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175–185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.作者: 即席 時間: 2025-3-22 08:27
rom sperm samples, and for selecting morphologically normal motile sperm. The methods that are described here include: ordinary centrifugation, sperm swim-up, glass wool and Sephadex filtration/adherence, and single-layer as well as discontinuous two-layer iodixanol density gradient centrifugation.作者: 萬神殿 時間: 2025-3-22 08:49
1064-3745 ation advice from the experts.Includes supplementary materia.This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-dr作者: ARBOR 時間: 2025-3-22 13:35 作者: ARBOR 時間: 2025-3-22 17:42
Book 2015Latest editionrs focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written作者: 空氣 時間: 2025-3-23 00:51 作者: 白楊魚 時間: 2025-3-23 03:20 作者: 合法 時間: 2025-3-23 09:29
he different cooling rates needed in different temperature regimes during the freezing process are being described. Cryopreservation procedures are described in case of using both specialized automated equipment and simple equipment.作者: enmesh 時間: 2025-3-23 13:10
Laser Scanning Microscopy in Cryobiologyuring freezing and thawing of a sample using a temperature-controlled sample holder. In this chapter, various advanced cryoimaging methods are described. Special emphasis is given on the different imaging modalities that can be used to study the various aspects of cryopreservation.作者: gerontocracy 時間: 2025-3-23 16:50
Low-Temperature Electron Microscopy: Techniques and Protocolshich are commonly encountered in more conventional room temperature electron microscopy techniques which rely heavily on chemical fixation and heavy metal staining. Often, cryo-electron microscopy allows direct observation of specimens, which have not been stained or chemically fixed.作者: 煩人 時間: 2025-3-23 20:10 作者: Expiration 時間: 2025-3-23 23:37
Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.作者: 聾子 時間: 2025-3-24 04:06
Efficient Cryopreservation of Human Pluripotent Stem Cells by Surface-Based Vitrificationon a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175–185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.作者: Accrue 時間: 2025-3-24 08:30 作者: 使隔離 時間: 2025-3-24 11:54
Chenshu Wu,Zheng Yang,Yunhao Liucribed. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.作者: exophthalmos 時間: 2025-3-24 15:41 作者: fulmination 時間: 2025-3-24 20:49
Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cellscribed. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.作者: 易彎曲 時間: 2025-3-24 23:35 作者: 變形 時間: 2025-3-25 06:40 作者: BUCK 時間: 2025-3-25 08:23 作者: Gudgeon 時間: 2025-3-25 12:39 作者: 心神不寧 時間: 2025-3-25 19:28 作者: 令人悲傷 時間: 2025-3-25 21:25 作者: 威脅你 時間: 2025-3-26 03:47
Timothy Kerswell,Surendra Pratap to investigate crystallization, eutectic formation, glass transition, devitrification, recrystallization, melting, polymorphism, molecular relaxation, phase separation, water transport, thermochemistry, and kinetics of complex reactions (e.g., protein denaturation). Such information can be used for作者: 助記 時間: 2025-3-26 04:59
Timothy Kerswell,Surendra Prataping procedures for the cryopreservation of cells and tissue. Conventional cryomicroscopic assays, which rely on indirect evidence of intracellular freezing (e.g., opacity changes in the cell cytoplasm), can yield significant errors in the estimated kinetics. In contrast, the formation and growth of 作者: DIKE 時間: 2025-3-26 11:14
Timothy Kerswell,Surendra Prataplities including Raman spectroscopy, fluorescence correlation spectroscopy, fluorescence lifetime imaging, or multiphoton imaging. Multiphoton imaging can be used to study intracellular ice formation at the subcellular level. A Raman imaging modality can be used for chemical mapping of frozen sample作者: V切開 時間: 2025-3-26 16:32
Timothy Kerswell,Surendra Pratapts components through the use of low temperature as a physical fixation strategy” (Steinbrecht and Zierold, Cryotechniques in biological electron microscopy. Springer-Verlag, Berlin, p 293, 1987). The intention is to maintain confidence that the tissue observed retains the morphology and dimensions 作者: 胡言亂語 時間: 2025-3-26 19:24 作者: 流浪者 時間: 2025-3-26 22:56 作者: 要塞 時間: 2025-3-27 02:32 作者: thrombus 時間: 2025-3-27 08:25 作者: 桶去微染 時間: 2025-3-27 10:18 作者: HAIL 時間: 2025-3-27 14:40 作者: hardheaded 時間: 2025-3-27 18:16 作者: Cabinet 時間: 2025-3-28 01:47 作者: happiness 時間: 2025-3-28 03:08
Radio Map Construction Without Site Surveystrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements necessary for the commercial exploitation of freeze-dried products.作者: 鉤針織物 時間: 2025-3-28 09:21 作者: Albumin 時間: 2025-3-28 12:40
The Principles of Freeze-Dryingstrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements necessary for the commercial exploitation of freeze-dried products.作者: 四指套 時間: 2025-3-28 15:49 作者: 協(xié)定 時間: 2025-3-28 21:50 作者: CROAK 時間: 2025-3-29 00:18 作者: Legend 時間: 2025-3-29 03:26
https://doi.org/10.1007/978-1-4939-2193-5Cryopreservation; calorimetric methods; cellular changes; freeze-drying; macromolecular changes; microsco作者: tariff 時間: 2025-3-29 09:30
978-1-4939-4912-0Springer Science+Business Media New York 2015作者: –scent 時間: 2025-3-29 14:52 作者: Pageant 時間: 2025-3-29 17:58
Principles of Cryopreservation by Vitrificationem have been discovered over the past 30+ years, and the former problem can in most cases be eliminated or adequately controlled by careful attention to technique. Vitrification is therefore beginning to realize its potential for enabling the superior and convenient cryopreservation of most types of作者: STYX 時間: 2025-3-29 23:48 作者: Dictation 時間: 2025-3-30 03:02 作者: 擁擠前 時間: 2025-3-30 06:16 作者: crucial 時間: 2025-3-30 11:36
Book 2015Latest editionative and practical, .Cryopreservation and Freeze-Drying Protocols, Third Edition. serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens..作者: 譏諷 時間: 2025-3-30 15:58
Chenshu Wu,Zheng Yang,Yunhao Liuable they must be able to penetrate into the cells and have low toxicity. Many compounds have such properties, including glycerol, dimethyl sulfoxide, ethanediol, and propanediol..In fact, both damaging mechanisms are important, their relative contributions depending on cell type, cooling rate, and 作者: Forehead-Lift 時間: 2025-3-30 18:33 作者: 分解 時間: 2025-3-30 21:41 作者: ABIDE 時間: 2025-3-31 04:30 作者: grotto 時間: 2025-3-31 05:08 作者: BINGE 時間: 2025-3-31 12:22 作者: Initial 時間: 2025-3-31 16:40 作者: exhibit 時間: 2025-3-31 18:51
Principles of Cryopreservation by Vitrificationof ice. Vitrification simplifies and frequently improves cryopreservation because it eliminates mechanical injury from ice, eliminates the need to find optimal cooling and warming rates, eliminates the importance of differing optimal cooling and warming rates for cells in mixed cell type populations
