標(biāo)題: Titlebook: Cryopreservation and Freeze-Drying Protocols; John G. Day,Glyn N. Stacey Book 2007Latest edition Humana Press 2007 biological materials.bi [打印本頁(yè)] 作者: genial 時(shí)間: 2025-3-21 19:11
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書目名稱Cryopreservation and Freeze-Drying Protocols讀者反饋學(xué)科排名
作者: MOCK 時(shí)間: 2025-3-21 20:48 作者: CHOIR 時(shí)間: 2025-3-22 03:32
In Vivo Dosimetry in Total Body Irradiationes after storage are minimal. Yeast strains have remained viable for more than 30 yr when stored using this method and sufficient cells are recovered to produce new working stocks. Although survival rates are strain specific, nearly all National Collection of Yeast Cultures strains covering most yea作者: collateral 時(shí)間: 2025-3-22 08:25 作者: 工作 時(shí)間: 2025-3-22 09:43
Application of HTA in Optometryore plunging the samples directly into liquid nitrogen to achieve vitrification. Extensive success has been achieved with both techniques but rapid cooling is, currently, widely favored because of its simplicity.作者: Affectation 時(shí)間: 2025-3-22 14:07 作者: Affectation 時(shí)間: 2025-3-22 19:30
1064-3745 notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources..978-1-61737-528-6978-1-59745-362-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Hangar 時(shí)間: 2025-3-22 22:39
Principles of Cryopreservation,able they must be able to penetrate into the cells and have low toxicity. Many compounds have such properties, including glycerol, dimethyl sulfoxide, ethanediol, and propanediol..In fact, both damaging mechanisms are important, their relative contributions depending on cell type, cooling rate, and 作者: Asparagus 時(shí)間: 2025-3-23 01:44
Freeze-Drying of Yeast Cultures,es after storage are minimal. Yeast strains have remained viable for more than 30 yr when stored using this method and sufficient cells are recovered to produce new working stocks. Although survival rates are strain specific, nearly all National Collection of Yeast Cultures strains covering most yea作者: 不足的東西 時(shí)間: 2025-3-23 09:29 作者: 絆住 時(shí)間: 2025-3-23 09:53 作者: Arthr- 時(shí)間: 2025-3-23 16:04
Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use,utologous collections that are stored prior to a planned infusion following further treatment of the patient or at the time of a subsequent relapse. Other autologous harvests are stored as backup or “rainy day” harvests, the former specifically being intended to rescue patients who develop graft fai作者: antedate 時(shí)間: 2025-3-23 20:36
The Principles of Freeze-Drying,ustrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements necessary for the commercial exploitation of freeze-dried products.作者: Iatrogenic 時(shí)間: 2025-3-24 02:14 作者: 使苦惱 時(shí)間: 2025-3-24 02:32 作者: AV-node 時(shí)間: 2025-3-24 07:59 作者: 釋放 時(shí)間: 2025-3-24 12:35 作者: 吸引力 時(shí)間: 2025-3-24 18:09 作者: botany 時(shí)間: 2025-3-24 20:08 作者: 跟隨 時(shí)間: 2025-3-25 02:38 作者: 劇毒 時(shí)間: 2025-3-25 05:09
John G. Day,Glyn N. StaceyBackground and explanatory notes for each protocol.Methods for lyophilization of proteins.Cutting-edge methods for conservation and sustainable use of resources.New and novel techniques for preserving作者: Terrace 時(shí)間: 2025-3-25 11:20 作者: 平淡而無(wú)味 時(shí)間: 2025-3-25 13:20 作者: 匯總 時(shí)間: 2025-3-25 18:39
Takaoki Takanashi,Hiraku Kawamuraustrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements necessary for the commercial exploitation of freeze-dried products.作者: 一起平行 時(shí)間: 2025-3-25 23:11 作者: 巡回 時(shí)間: 2025-3-26 02:22
Lejla Gurbeta,Zijad D?emic,Almir Badnjevic by the purification of a protein intermediate as part of the analytical characterization of a protein, and at intermediate scale, as illustrated by the pilot development of a lyophilized protein reference material such as for use in bioassay or immunoassay. Advice on common problems with freeze-dry作者: 加劇 時(shí)間: 2025-3-26 07:58
In Vivo Dosimetry in Total Body Irradiationntain a small number of strains by serial transfer, such methods are not the best, particularly for long-term storage or for keeping a large collection. One of the major problems is dealing with the vast range of organisms that have been isolated to date. Particularly in the case of methods of culti作者: 有惡意 時(shí)間: 2025-3-26 12:05
In Vivo Dosimetry in Total Body Irradiation in a two-stage process. Yeast cultures are placed in glass ampoules after having been mixed with a lyoprotectant. Primary drying is carried out using a centrifuge head connected to a standard freeze-dryer. Once the centrifuge head is running, air is removed and evaporated liquid is captured in the 作者: Gratuitous 時(shí)間: 2025-3-26 16:29 作者: 流行 時(shí)間: 2025-3-26 17:11 作者: 補(bǔ)充 時(shí)間: 2025-3-26 22:20
In Vivo Dosimetry in Total Body Irradiationumerous methodologies available to preserve a fungus, but the two methods widely used by culture collections (biological or genetic resource centers) to achieve successful preservation are cryopreservation with liquid nitrogen using controlled-rate freezing and centrifugal freeze-drying. Generic met作者: Incompetent 時(shí)間: 2025-3-27 02:00
Application of HTA in Optometrytic algae can also be cryopreserved, but typically with lower post-thaw viability levels. However, to date, most dinoflagellates, cryptophytes, synurophytes, and raphidophytes cannot be successfully cryopreserved. Marine diatoms can be cryopreserved, and often have high viability, although freshwate作者: Rebate 時(shí)間: 2025-3-27 07:28
Application of HTA in Optometrytion of higher plant germplasm. Suspension cultures are also important in biotechnology, particularly in transformation studies and for the production of specific metabolites, and, here, there is also a pressing need for genetically stable, long-term storage of cell lines..Cryopreservation of suspen作者: Bouquet 時(shí)間: 2025-3-27 12:15 作者: 模仿 時(shí)間: 2025-3-27 15:23
Convergence of Wavelet Packet Series,er contents below the high-moisture freezing limit; and transfer and maintenance at a subzero temperature that may be optimized in relation to the seed-lot moisture content and species. Temperatures about 70°C below the glass transition temperature (Tg–70) or lower are recommended for seed storage. 作者: Expressly 時(shí)間: 2025-3-27 21:10
Applications in Image Processing,es, but details are also given for individual fish species. Successful cryopreservation of fish spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration conditions, choice of cryoprotectant medium, cooling/thawing regimes, and conditions for fertilizati作者: Musket 時(shí)間: 2025-3-27 22:59
Applications in Image Processing,nto liquid nitrogen with dimethylacetamide as cryoprotectant. The method originates from the group at the Research Institute of Farm Animal Breeding and Genetics at St. Petersburg-Pushkin and is described here for chicken spermatozoa, but has also been adapted successfully for other species, such as作者: Gastric 時(shí)間: 2025-3-28 03:29 作者: 思想 時(shí)間: 2025-3-28 09:29
Chenshu Wu,Zheng Yang,Yunhao Liucal malignancies. At least 70% of these are autologous transplants, the remaining 30% being allogeneic, which are sourced from related (70% of the allogeneic) or unrelated donors. Peripheral blood mobilized with granulocyte colony stimulating factor is the major source of stem cells for transplantat作者: plasma-cells 時(shí)間: 2025-3-28 11:48 作者: Crumple 時(shí)間: 2025-3-28 15:03 作者: Arb853 時(shí)間: 2025-3-28 21:20 作者: FIG 時(shí)間: 2025-3-28 23:59 作者: 出來(lái) 時(shí)間: 2025-3-29 04:50
Principles of Cryopreservation, and this chapter seeks to analyze some of the mechanisms involved and to show how cooling can be used to produce stable conditions that preserve life. The biological effects of cooling are dominated by the freezing of water, which results in the concentration of the solutes that are dissolved in th作者: 開始沒(méi)有 時(shí)間: 2025-3-29 10:26
Lyophilization of Proteins, by the purification of a protein intermediate as part of the analytical characterization of a protein, and at intermediate scale, as illustrated by the pilot development of a lyophilized protein reference material such as for use in bioassay or immunoassay. Advice on common problems with freeze-dry作者: ORE 時(shí)間: 2025-3-29 12:55
Vacuum-Drying and Cryopreservation of Prokaryotes,ntain a small number of strains by serial transfer, such methods are not the best, particularly for long-term storage or for keeping a large collection. One of the major problems is dealing with the vast range of organisms that have been isolated to date. Particularly in the case of methods of culti作者: 事物的方面 時(shí)間: 2025-3-29 19:06 作者: 一起平行 時(shí)間: 2025-3-29 21:34 作者: 送秋波 時(shí)間: 2025-3-30 02:04 作者: scoliosis 時(shí)間: 2025-3-30 04:55
Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying,umerous methodologies available to preserve a fungus, but the two methods widely used by culture collections (biological or genetic resource centers) to achieve successful preservation are cryopreservation with liquid nitrogen using controlled-rate freezing and centrifugal freeze-drying. Generic met作者: 泰然自若 時(shí)間: 2025-3-30 10:04
Cryopreservation of Microalgae and Cyanobacteria,tic algae can also be cryopreserved, but typically with lower post-thaw viability levels. However, to date, most dinoflagellates, cryptophytes, synurophytes, and raphidophytes cannot be successfully cryopreserved. Marine diatoms can be cryopreserved, and often have high viability, although freshwate作者: 起波瀾 時(shí)間: 2025-3-30 14:52
Cryopreservation of Plant Cell Suspensions,tion of higher plant germplasm. Suspension cultures are also important in biotechnology, particularly in transformation studies and for the production of specific metabolites, and, here, there is also a pressing need for genetically stable, long-term storage of cell lines..Cryopreservation of suspen作者: 熒光 時(shí)間: 2025-3-30 17:01 作者: 招致 時(shí)間: 2025-3-30 22:14 作者: BUDGE 時(shí)間: 2025-3-31 04:48 作者: 熔巖 時(shí)間: 2025-3-31 06:02
Cryopreservation of Avian Spermatozoa,nto liquid nitrogen with dimethylacetamide as cryoprotectant. The method originates from the group at the Research Institute of Farm Animal Breeding and Genetics at St. Petersburg-Pushkin and is described here for chicken spermatozoa, but has also been adapted successfully for other species, such as作者: 支柱 時(shí)間: 2025-3-31 09:25
Cryopreservation of Animal and Human Cell Lines,intain the increasing numbers of cell lines, as they started to emerge from research laboratories from the mid-1900s, necessitated a radical approach to the problem. The realization that they could survive cryopreservation, and that a slow rate of cooling is essential for this survival, led to the e作者: blister 時(shí)間: 2025-3-31 14:22
Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use,cal malignancies. At least 70% of these are autologous transplants, the remaining 30% being allogeneic, which are sourced from related (70% of the allogeneic) or unrelated donors. Peripheral blood mobilized with granulocyte colony stimulating factor is the major source of stem cells for transplantat作者: prodrome 時(shí)間: 2025-3-31 20:07
Cryopreservation of Human Embryonic Stem Cell Lines,/rapid warming. The vitrification method described here is designed for hESCs that grow as discrete colonies on a feeder cell monolayer, and are subcultured by manual subdivision of the colonies into multicellular clumps. hESCs that are subcultured by enzymatic dissociation can more conveniently be 作者: 噴出 時(shí)間: 2025-4-1 01:41 作者: 致詞 時(shí)間: 2025-4-1 03:38
Book 2007Latest edition of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995...This volume begins with a discussion of long term ex situ conservation of biological resources, the 作者: PRO 時(shí)間: 2025-4-1 06:25 作者: 元音 時(shí)間: 2025-4-1 10:31 作者: STALL 時(shí)間: 2025-4-1 17:22 作者: BRACE 時(shí)間: 2025-4-1 20:51 作者: 支形吊燈 時(shí)間: 2025-4-1 23:31
Cryopreservation of Animal and Human Cell Lines,ventual discovery of cryoprotectants. Subsequent development of mechanical freezers, which can accurately control the rate of cooling, now allows cells to be cryopreserved at their maximum viability. This chapter outlines the essentials steps for the successful preparation, freezing, and storage of cell lines.
