標(biāo)題: Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich F?rster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s), [打印本頁] 作者: Garfield 時間: 2025-3-21 17:34
書目名稱Cryo-Electron Tomography影響因子(影響力)
作者: 斜 時間: 2025-3-22 00:09
Petra Lietz,Molie Tobin,Dita Nugroho. We then provide a comprehensive account of the current state-of-the-art cryo-ET?sample preparation techniques for specimens?ranging from in-vitro reconstitutions, single cells, to 3D cell cultures, and whole multicellular organisms. We point out specific considerations that facilitate a wide range作者: 使痛苦 時間: 2025-3-22 03:55 作者: 永久 時間: 2025-3-22 05:07 作者: Constitution 時間: 2025-3-22 09:17 作者: 壯觀的游行 時間: 2025-3-22 16:18 作者: 壯觀的游行 時間: 2025-3-22 20:36
Shauna Butterwick,Marilou Carrilloachine learning and advanced algorithms, and discuss challenges posed by the recent developments of deep learning methods. New approaches for localization of molecular complexes and spatial analysis are expected to provide precise molecular maps of diverse cellular compartments, elucidate the organi作者: immunity 時間: 2025-3-23 00:15
Telling Old Stories in New Waysg a wider context with equal detail. Electron tomography can also be performed on serial sections which allows entire cells or tissue volumes to be reconstructed at high resolution. Further, high precision correlative microscopy workflows can be implemented, permitting to combine information on mole作者: muffler 時間: 2025-3-23 04:28
Fundamentals of Instrumentation and Electron Optics for Cryo-Electron Tomography, eventually recorded in the image plane—by ever more sophisticated detectors. As we acknowledge that our sample fries all too quickly, we l also discuss low dose techniques and phase plates. Finally, we provide a short outlook towards exciting developments that may well push cryo-electron tomography作者: fulmination 時間: 2025-3-23 08:48 作者: KEGEL 時間: 2025-3-23 11:05
Cryo-Focused Ion Beam Milling of Cells,ity to frozen cells. Preparing samples using FIB milling of frozen-hydrated cells at cryogenic temperatures is known as cryo-FIB milling, which has emerged as one of the gold-standard techniques for cellular cryo-ET to generate molecular landscapes inside cells. In this chapter, we present a brief h作者: lethal 時間: 2025-3-23 15:05
In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques,nd higher throughout. The following chapter provides a topical overview of this new and rapidly developing technique. We introduce the basic principles of cryo-lift out and briefly summarize the technological and biological results so far.作者: 喧鬧 時間: 2025-3-23 18:38 作者: INCH 時間: 2025-3-24 00:26
Beam-Induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electro” response, having both a repeated elastic component and a plastic deformation. The elastic deformation represents the portion that can be fully relaxed when the electron beam is turned off at the end of the exposure for each tilted image. By contrast, the plastic deformation decays exponentially wi作者: 連鎖 時間: 2025-3-24 04:14
Detection and Interpretation of Cellular Structures in Tomograms: Segmentation, Localization and Spachine learning and advanced algorithms, and discuss challenges posed by the recent developments of deep learning methods. New approaches for localization of molecular complexes and spatial analysis are expected to provide precise molecular maps of diverse cellular compartments, elucidate the organi作者: Inelasticity 時間: 2025-3-24 09:41
Electron Tomography of Cryo-Fixed and Resin-Embedded Samples,g a wider context with equal detail. Electron tomography can also be performed on serial sections which allows entire cells or tissue volumes to be reconstructed at high resolution. Further, high precision correlative microscopy workflows can be implemented, permitting to combine information on mole作者: Clinch 時間: 2025-3-24 12:22
Book 2024 from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, 作者: Malaise 時間: 2025-3-24 17:06
1571-4853 sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, 978-3-031-51173-8978-3-031-51171-4Series ISSN 1571-4853 Series E-ISSN 2542-9566 作者: aggressor 時間: 2025-3-24 19:04 作者: Disk199 時間: 2025-3-25 01:32 作者: mitten 時間: 2025-3-25 04:28 作者: OTHER 時間: 2025-3-25 09:42
A Mother/Daughter Distance Danceetation of data from cryo-ET: correlative light and electron microscopy (CLEM) and molecular tagging. These methods can be used independently or together and help cryo-ET practitioners identify areas for data collection and localise or identify specific molecules in their reconstructed tomograms.作者: 臨時抱佛腳 時間: 2025-3-25 14:25
A Mother/Daughter Distance Danceearly 20?years’ worth of cryo-ET data from our own research group to highlight general challenges and solutions for democratizing cryo-ET data and making it Findable, Accessible, Interoperable and Reusable long-term.作者: mendacity 時間: 2025-3-25 18:08
Targeting, Localisation and Identification in Cryo-ET,etation of data from cryo-ET: correlative light and electron microscopy (CLEM) and molecular tagging. These methods can be used independently or together and help cryo-ET practitioners identify areas for data collection and localise or identify specific molecules in their reconstructed tomograms.作者: antiandrogen 時間: 2025-3-25 20:03 作者: 新娘 時間: 2025-3-26 01:20 作者: 阻塞 時間: 2025-3-26 07:55 作者: 館長 時間: 2025-3-26 11:45
Book 2024the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that 作者: 支形吊燈 時間: 2025-3-26 13:48 作者: Recessive 時間: 2025-3-26 17:23
978-3-031-51173-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl作者: 不能仁慈 時間: 2025-3-27 00:55
Cryo-Electron Tomography978-3-031-51171-4Series ISSN 1571-4853 Series E-ISSN 2542-9566 作者: infatuation 時間: 2025-3-27 03:50
Sharifah Norul Akmar Syed Zamrihy. Before we throw ourselves into the fray, we are briefly soaking in some information on notable development milestones of the past. I firmly believe there is value in appreciating the historical context of electron microscopy to gain a better understanding of the many advances that were necessary作者: DEAF 時間: 2025-3-27 08:40 作者: 粉筆 時間: 2025-3-27 10:41 作者: accrete 時間: 2025-3-27 14:30 作者: Overdose 時間: 2025-3-27 20:00
Lei Mee Thien,I Gusti Ngurah Darmawaned structures is rapidly expanding and requires that preparation techniques evolve to keep up with the growing demand for larger specimens. In particular, organoids and tissue, but also small animals that can be vitrified in whole by high-pressure freezing, are targets of this new branch of in situ 作者: Sinus-Node 時間: 2025-3-27 22:47 作者: 小教堂 時間: 2025-3-28 03:27
A Mother/Daughter Distance Danceicroscope (TEM). This chapter describes the process of collecting consecutive images of the tilted specimen efficiently in theory and practice. Firstly, the concept of a TEM and its main components are described prior to introducing the technical means to maintain an area of interest in the field of作者: obscurity 時間: 2025-3-28 07:14 作者: Fibroid 時間: 2025-3-28 14:17 作者: Custodian 時間: 2025-3-28 14:50
Shauna Butterwick,Marilou Carrillomolecular complexes. However, extracting this information is particularly challenging for complexes that cannot be identified visually and are embedded in cellular environments comprising multitude of macromolecular?species that lack an obvious ordering pattern. To overcome these challenges, a numbe作者: sundowning 時間: 2025-3-28 21:56 作者: intoxicate 時間: 2025-3-29 01:55
A Mother/Daughter Distance Dancenly sharing such data enables validation of research findings, sparks further biological discoveries, and fuels development of improved software tools for data processing. Data sharing is complicated, however, by the size of cryo-ET datasets. Here we synthesize our experience archiving and sharing n作者: AMEND 時間: 2025-3-29 05:16
Friedrich F?rster,Ariane BriegelComprehensive description of cryo-electron tomography.Addresses the underlying principles and all recent developments of cryo-ET.A valuable resource for scientists working in the field of structural b作者: 粗野 時間: 2025-3-29 09:51 作者: 高度 時間: 2025-3-29 13:35 作者: overreach 時間: 2025-3-29 17:23
Principles of Tomographic Reconstruction,sive imaging method, two-dimensional (2D) images of slices, or sections, within an organism are analyzed to determine the three-dimensional (3D) localization of internal objects. These sections can be so thin that the target objects are unobscured by the superposition of over- or underlying structur作者: 疏遠(yuǎn)天際 時間: 2025-3-29 20:32 作者: BLA 時間: 2025-3-30 02:11 作者: ethnology 時間: 2025-3-30 07:06
In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques,ed structures is rapidly expanding and requires that preparation techniques evolve to keep up with the growing demand for larger specimens. In particular, organoids and tissue, but also small animals that can be vitrified in whole by high-pressure freezing, are targets of this new branch of in situ 作者: 獨(dú)特性 時間: 2025-3-30 09:48
Targeting, Localisation and Identification in Cryo-ET,sequence of this direct imaging is that every molecule in the sample contributes to the resulting tomogram, and for complex specimens such as cells or viruses, it can be challenging to identify a specific feature of interest. This chapter discusses two approaches to help guide collection and interpr
