標(biāo)題: Titlebook: Craniofacial Development; Methods and Protocol Sebastian Dworkin Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁] 作者: exterminate 時間: 2025-3-21 17:04
書目名稱Craniofacial Development影響因子(影響力)
作者: 書法 時間: 2025-3-21 22:58
Book 2022oaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (.c. elegans., zebrafish, mouse, alongside human imaging approaches), together with cell cultu作者: 美麗的寫 時間: 2025-3-22 01:05 作者: RACE 時間: 2025-3-22 07:50 作者: 效果 時間: 2025-3-22 11:15
Wavelets in h?heren Dimensionenangeable between various vertebrate species, and between various surface imaging techniques (including micro-CT and 3D surface scanners), offering a high level of versatility and precision for extensive phenotyping of the entire craniofacial complex.作者: EWE 時間: 2025-3-22 14:00
Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue,ate culturing tissue from developmental time-points as early as embryonic day 10.5. This allows for greater control over genotypic variance within litters and provides a simplified, accessible methodology.作者: EWE 時間: 2025-3-22 20:16
Single Cell RNA-Seq: Cell Isolation and Data Analysis, present a streamlined protocol to isolate fresh cells from tissues in vivo. We also share extensive notes to highlight considerations researchers should take into account before beginning their cell isolation protocol.作者: Additive 時間: 2025-3-22 22:36
Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofaciangeable between various vertebrate species, and between various surface imaging techniques (including micro-CT and 3D surface scanners), offering a high level of versatility and precision for extensive phenotyping of the entire craniofacial complex.作者: SCORE 時間: 2025-3-23 03:34 作者: 軍械庫 時間: 2025-3-23 06:54 作者: tangle 時間: 2025-3-23 12:18
Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton,ful stains for stromal/mesenchymal tissues including bone and cartilage. Techniques are tailored to decalcified, paraffin-embedded mouse tissue; however, these methods are applicable under a broad range of conditions.作者: seruting 時間: 2025-3-23 17:44 作者: STING 時間: 2025-3-23 18:58 作者: 有惡意 時間: 2025-3-23 23:48
Wavelets in h?heren Dimensionen (wild type, morphants, mutants, transgenic, or pharmacologically treated animals as well as all of their controls), provided the sample size is kept under a limit. Thus, we hope to encourage researchers to use microanatomy and histology to complement molecular studies investigating, e.g., gene function.作者: GRAZE 時間: 2025-3-24 04:59
Visualization of the Cartilage and Bone Elements in the Craniofacial Structures by Alcian Blue and nd space will identify structural defects of head structures and guide follow-up analysis of the molecular and cellular attributes associated with the defects. Here we describe a protocol to simultaneously visualize the cartilage and bone elements by Alcian blue and Alizarin red staining, respectively, of wholemount specimens in mouse models.作者: 暫時過來 時間: 2025-3-24 09:37
High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models, (wild type, morphants, mutants, transgenic, or pharmacologically treated animals as well as all of their controls), provided the sample size is kept under a limit. Thus, we hope to encourage researchers to use microanatomy and histology to complement molecular studies investigating, e.g., gene function.作者: scoliosis 時間: 2025-3-24 13:59 作者: Pathogen 時間: 2025-3-24 16:23
Kompression und Unterdrückung von Rauschen on the complementary binding (hybridization) of the DNA/RNA probe to the target transcript. The bound probe can then be visualized by an enzymatic color reaction to delineate the expression pattern of transcripts within a tissue. Here we describe an optimized method to perform in situ hybridization in mouse embryos.作者: Airtight 時間: 2025-3-24 22:26 作者: Expostulate 時間: 2025-3-24 23:12 作者: JIBE 時間: 2025-3-25 03:21 作者: Interdict 時間: 2025-3-25 09:40 作者: 萬靈丹 時間: 2025-3-25 14:35
Die kontinuierliche Wavelet-Transformationons during development can lead to dysmorphology of the skull, jaw, and the pharyngeal structures. Developmental disorders can be investigated in animal models to elucidate the molecular and cellular consequences of the morphogenetic defects. A first step in determining the disruption in the develop作者: Substance-Abuse 時間: 2025-3-25 19:16 作者: 遠(yuǎn)地點 時間: 2025-3-25 23:22
Schnelle numerische lineare Algebramon birth defects globally, with great scope for future research in both normal and dysmorphic palatogenesis. This process can be studied in the mouse model using both the hyperoxic rolling culture of maxillary explants and Trowell-type static cultures, which are optimal for the study of different s作者: 宴會 時間: 2025-3-26 00:54 作者: epicondylitis 時間: 2025-3-26 06:24 作者: 政府 時間: 2025-3-26 11:41
Wavelets in h?heren Dimensionen our understanding of growth and development in health and disease. Three-dimensional (3D) imaging has played a key role in advancing craniofacial phenomics by facilitating highly sensitive and specific characterizations of craniofacial and dental morphology. Here we describe the use of micro-comput作者: 聯(lián)想記憶 時間: 2025-3-26 14:53
Wavelets in h?heren Dimensionen While standardized protocols exist for micro-CT–based microarchitecture assessment of long bones, specific protocols need to be developed for different types of skull bones taking into account differences in embryogenesis, organization, development, and growth compared to the rest of the body. This作者: 象形文字 時間: 2025-3-26 19:23
Die kontinuierliche Wavelet-Transformatione concerted activity of multiple enhancers driving cell type-specific expression of a target gene. Mammalian genomes are organized in topologically associated domains, providing a preferred environment and framework for interactions between transcriptional enhancers and gene promoters. While epigeno作者: Fantasy 時間: 2025-3-27 01:01
Kompression und Unterdrückung von Rauschentem. This chapter provides the craniofacial biologist with an introduction to tissue harvesting, embedding, and sectioning as well as a toolkit of useful stains for stromal/mesenchymal tissues including bone and cartilage. Techniques are tailored to decalcified, paraffin-embedded mouse tissue; howev作者: 傾聽 時間: 2025-3-27 03:37 作者: 有惡臭 時間: 2025-3-27 05:55
Wavelets in h?heren Dimensionen the molecular biology ex vivo. The use of cell cultures by bone biologists to investigate pathological bone formation has been well established (Marie et al. Vitro Cell Dev Biol 25:373–380, 1989), and the use of cell culture techniques was subsequently applied to investigate craniosynostosis (Marie作者: cocoon 時間: 2025-3-27 10:26
Die kontinuierliche Wavelet-Transformationfects. Here we describe the general fabrication and modification of hydrogels, cryogels, and electrospun scaffolds. These scaffolds offer a variety of templates for facilitating bone growth and regeneration in craniofacial applications.作者: incision 時間: 2025-3-27 14:27 作者: enlist 時間: 2025-3-27 19:40 作者: Cholesterol 時間: 2025-3-28 00:24 作者: CUMB 時間: 2025-3-28 02:29
Schnelle numerische lineare Algebrayme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture, allowing access to the tissue to follow d作者: Kidney-Failure 時間: 2025-3-28 08:21
Craniofacial Development978-1-0716-1847-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 壓艙物 時間: 2025-3-28 10:52 作者: gratify 時間: 2025-3-28 15:51
Kompression und Unterdrückung von RauschenWholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5?days postfertilization.作者: quiet-sleep 時間: 2025-3-28 22:46
Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development,Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5?days postfertilization.作者: 瘙癢 時間: 2025-3-29 00:52
Sebastian DworkinIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Psychogenic 時間: 2025-3-29 03:33
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/239284.jpg作者: 博識 時間: 2025-3-29 09:54
Scaffolds for Use in Craniofacial Bone Regeneration,fects. Here we describe the general fabrication and modification of hydrogels, cryogels, and electrospun scaffolds. These scaffolds offer a variety of templates for facilitating bone growth and regeneration in craniofacial applications.作者: exostosis 時間: 2025-3-29 13:54 作者: Meander 時間: 2025-3-29 16:28
978-1-0716-1849-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 小卷發(fā) 時間: 2025-3-29 22:10
Using , as a Model for Mechanistic Insights of Craniofacial Development,es that are associated with human disorders. It is well established that evolution has conserved critical domains of proteins and their cellular functions even though the phenotypic output for analogous mutations can be distinct among organisms. To that end, the genes that are associated with human 作者: 簡略 時間: 2025-3-30 03:08
Elucidation of Gene Expression Patterns in the Craniofacial Tissues of Mouse Embryos by Wholemount (WISH) is an informative technique to visualize gene expression in tissues across the developmental stages of embryos. The principle of WISH is based on the complementary binding (hybridization) of the DNA/RNA probe to the target transcript. The bound probe can then be visualized by an enzymatic co作者: 顯微鏡 時間: 2025-3-30 07:39
Visualization of the Cartilage and Bone Elements in the Craniofacial Structures by Alcian Blue and ons during development can lead to dysmorphology of the skull, jaw, and the pharyngeal structures. Developmental disorders can be investigated in animal models to elucidate the molecular and cellular consequences of the morphogenetic defects. A first step in determining the disruption in the develop作者: 簡略 時間: 2025-3-30 10:43
Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue,nd multifactorial nature of their etiology. In vitro modeling of craniofacial morphogenesis provides valuable insight into the developmental processes critical to the presentation of these conditions. One such technique, termed a submerged or free-floating organ culture, allows culturing and observa作者: 灰心喪氣 時間: 2025-3-30 13:39 作者: flavonoids 時間: 2025-3-30 18:46
Single Cell RNA-Seq: Cell Isolation and Data Analysis, which tissue architecture can be characterized. Multiple cell capturing methods have been developed that, when coupled to next-generation sequencing, can yield cell-to-cell specific information regarding gene expression profiles. One of the commonalities between all of the cell capturing techniques作者: 使尷尬 時間: 2025-3-30 22:40 作者: 案發(fā)地點 時間: 2025-3-31 02:36 作者: 賭博 時間: 2025-3-31 05:03 作者: curriculum 時間: 2025-3-31 11:37 作者: lymphoma 時間: 2025-3-31 16:47 作者: 熱心 時間: 2025-3-31 20:18
,Engineering Epithelial–Mesenchymal Microtissues to Study Cell–Cell Interactions in Development,ining operationally simple and scalable. As an example, formation of the upper lip and palate requires the orchestrated proliferation and fusion of embryonic facial growth centers and is dependent on paracrine epithelial–mesenchymal signaling through multiple pathways including the Sonic Hedgehog (S作者: formula 時間: 2025-4-1 01:36
Ex Vivo Culture of Human Cranial Suture Cells, the molecular biology ex vivo. The use of cell cultures by bone biologists to investigate pathological bone formation has been well established (Marie et al. Vitro Cell Dev Biol 25:373–380, 1989), and the use of cell culture techniques was subsequently applied to investigate craniosynostosis (Marie作者: 我正派 時間: 2025-4-1 03:42
Scaffolds for Use in Craniofacial Bone Regeneration,fects. Here we describe the general fabrication and modification of hydrogels, cryogels, and electrospun scaffolds. These scaffolds offer a variety of templates for facilitating bone growth and regeneration in craniofacial applications.作者: 性上癮 時間: 2025-4-1 08:03 作者: DOLT 時間: 2025-4-1 13:49
High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models,ssion. We list a number of caveats and present a protocol for embedding, sectioning, and staining semithin plastic sections. The method is neither new nor innovative, but is meant to revive skills that tend to get lost..This easy-to-use and inexpensive protocol (1) yields high-resolution images in t作者: 擔(dān)憂 時間: 2025-4-1 17:39 作者: 騎師 時間: 2025-4-1 22:06
Salivary Gland Development in Culture,yme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture, allowing access to the tissue to follow d
