標(biāo)題: Titlebook: Confocal Microscopy; Methods and Protocol Stephen W. Paddock Book 19991st edition Humana Press 1999 [打印本頁] 作者: Cyclone 時(shí)間: 2025-3-21 18:37
書目名稱Confocal Microscopy影響因子(影響力)
書目名稱Confocal Microscopy影響因子(影響力)學(xué)科排名
書目名稱Confocal Microscopy網(wǎng)絡(luò)公開度
書目名稱Confocal Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Confocal Microscopy被引頻次
書目名稱Confocal Microscopy被引頻次學(xué)科排名
書目名稱Confocal Microscopy年度引用
書目名稱Confocal Microscopy年度引用學(xué)科排名
書目名稱Confocal Microscopy讀者反饋
書目名稱Confocal Microscopy讀者反饋學(xué)科排名
作者: interlude 時(shí)間: 2025-3-21 21:52
Preparation of Yeast Cells for Confocal Microscopy,copy has been used to examine the distribution of actin in fixed cells prepared from reverting protoplasts, and to show that a monoclonal antibody raised to rat liver nuclear proteins recognized two protein components of the yeast nuclear pore complex, p95 and p110 (.,.).作者: 路標(biāo) 時(shí)間: 2025-3-22 02:34
Confocal Microscopy on , Oocytes and Embryos,opy. Optical sectioning eliminates the need for manual sectioning and makes background fluorescence and autofluorescence negligible. It is still difficult to image the deep structures within the embryo, but thick wax sections can be cut and confocal microscopy again applied.作者: 紅腫 時(shí)間: 2025-3-22 06:03 作者: nitric-oxide 時(shí)間: 2025-3-22 09:59
Live Imaging with Green Fluorescent Protein,mit each stage of development to be studied in a single, intact embryo. Third, it would function in all cell types and would reveal their morphology, making it simple to identify different cells without compromising their viability.作者: 影響 時(shí)間: 2025-3-22 14:11 作者: 影響 時(shí)間: 2025-3-22 21:07 作者: 粗鄙的人 時(shí)間: 2025-3-22 23:29
copy has been used to examine the distribution of actin in fixed cells prepared from reverting protoplasts, and to show that a monoclonal antibody raised to rat liver nuclear proteins recognized two protein components of the yeast nuclear pore complex, p95 and p110 (.,.).作者: 亂砍 時(shí)間: 2025-3-23 04:10
opy. Optical sectioning eliminates the need for manual sectioning and makes background fluorescence and autofluorescence negligible. It is still difficult to image the deep structures within the embryo, but thick wax sections can be cut and confocal microscopy again applied.作者: 歌曲 時(shí)間: 2025-3-23 08:18
1064-3745 ques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, 作者: Occlusion 時(shí)間: 2025-3-23 10:33
dary antibodies and chromogenic substrates. The use of alkaline phosphatase substrates and their diffusible products limits the resolution of staining, particularly in thick tissues and deep within the embryo. Without additional probes, double-labeling and interpretation of results is also very difficult.作者: FLIT 時(shí)間: 2025-3-23 14:07 作者: accordance 時(shí)間: 2025-3-23 19:35 作者: hypnotic 時(shí)間: 2025-3-24 01:35 作者: 抒情短詩 時(shí)間: 2025-3-24 05:33
imaging cells deep within later stage embryos. Second, immunofluorescence and confocal detection allow the selective and simultaneous labeling with up to three different primary antisera. The following protocol contains instructions for the fixation, labeling, and detection of . embryos with one, two, and three different primary antisera.作者: 做作 時(shí)間: 2025-3-24 09:57
determine the genetic and epigenetic mechanisms that choreograph these events, it is often very useful to follow the morphogenetic behaviors of single cells and cell populations within the living zebrafish embryo.作者: 寡頭政治 時(shí)間: 2025-3-24 12:35 作者: 實(shí)施生效 時(shí)間: 2025-3-24 16:25
Imaging Gene Expression Using Antibody Probes, are physically labeled with dextran coupled to a fluorophore (.) or are labeled with fluorescent lipophilic dyes, such as diI (.). Multiple labeling experiments are then performed comparing the expression of these cell markers with endogenous protein(s) of interest.作者: integrated 時(shí)間: 2025-3-24 20:41 作者: 有幫助 時(shí)間: 2025-3-25 01:38
Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos, determine the genetic and epigenetic mechanisms that choreograph these events, it is often very useful to follow the morphogenetic behaviors of single cells and cell populations within the living zebrafish embryo.作者: 籠子 時(shí)間: 2025-3-25 04:57
Live Confocal Analysis with Fluorescently Labeled Proteins,diated phenotypes often can be accomplished only through live fluorescence analysis. Finally, live analysis has been used to confirm the existence of structures in the embryo that were previously contested as possible fixation artifacts (.).作者: nonchalance 時(shí)間: 2025-3-25 09:58
Book 19991st editionmens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.......作者: Obstacle 時(shí)間: 2025-3-25 15:10 作者: 聲明 時(shí)間: 2025-3-25 18:54
is biological arena is the tracking of calcium levels in different subcellular regions. Our goal is to consider how fluorescent calcium indicators, in conjunction with confocal microscopy, can best be used to image subcellular calcium dynamics.作者: inculpate 時(shí)間: 2025-3-25 22:28
Confocal Methods for ,,ltaneously. A confocal microscope equipped with a krypton/argon laser can simultaneously detect up to three different antigens. Using a confocal microscope it is also possible to collect a series of optical sections through a sample that allows observation of changes in distribution of the antigen in different focal planes of the tissue or cell.作者: 古董 時(shí)間: 2025-3-26 03:29 作者: 臭名昭著 時(shí)間: 2025-3-26 08:17
Book 19991st editionthe bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnic作者: BLOT 時(shí)間: 2025-3-26 08:46 作者: HOWL 時(shí)間: 2025-3-26 15:19
er scanning confocal microscope (LSCM) is somewhat better than that achieved in a conventional, wide-field light microscope (theoretical maximum resolution of 0.2 μm), but not as great as that in the transmission electron microscope (0.1 nm), it has bridged the gap between these two commonly used te作者: 讓空氣進(jìn)入 時(shí)間: 2025-3-26 18:12
structions can be made surpasses the limitations of conventional “wide-field” microscopic techniques where microtome sectioning is often required and cells must be viewed as flat, two-dimensional objects. Furthermore, the reduction in out-of-focus flare increases depth discrimination.作者: 出汗 時(shí)間: 2025-3-27 00:32 作者: 人類 時(shí)間: 2025-3-27 01:14
rvous system. A main theme in these examples is that the cell and tissue events are dynamic, on a time scale from minutes to days, covering spatial regions ranging in size from microns to millimeters.作者: 不規(guī)則的跳動(dòng) 時(shí)間: 2025-3-27 06:26
be either parameter insensitive, inversely sensitive, or exhibit a different sensitivity profile. Emission intensities for the two wavelengths are then divided by each other and thus the resulting ratio becomes normalized for inhomogeneities in probe distribution and concentration and in the system 作者: anniversary 時(shí)間: 2025-3-27 13:05 作者: 推延 時(shí)間: 2025-3-27 13:54 作者: 場所 時(shí)間: 2025-3-27 20:08 作者: evince 時(shí)間: 2025-3-27 21:56
Imaging Sea Urchin Fertilization,, as the morphological features of most types of animal eggs are extremely well suited to the specific advantages this technology offers. Confocal microscopy is a powerful and flexible tool, and has been skillfully used and developed by a number of researchers not only to study the structural featur作者: 厚臉皮 時(shí)間: 2025-3-28 04:37 作者: ostensible 時(shí)間: 2025-3-28 06:40
Intracellular pH and pCa Measurement,be either parameter insensitive, inversely sensitive, or exhibit a different sensitivity profile. Emission intensities for the two wavelengths are then divided by each other and thus the resulting ratio becomes normalized for inhomogeneities in probe distribution and concentration and in the system 作者: 過度 時(shí)間: 2025-3-28 11:13
Measuring Dynamic Cell Volume In Situ by Confocal Microscopy,ssues, the protocols can be applied to other intact animal, plant, and fungal tissues (.). As we show below, many factors must be taken into account in the choice of experimental parameters, there are no “best settings” that work for all tissues, in all cases.作者: Chauvinistic 時(shí)間: 2025-3-28 14:54 作者: 道學(xué)氣 時(shí)間: 2025-3-28 22:08
by imaging only one plane within the sample at a time so that variations in depth can be quantified (.). This has both positive and negative aspects. The advantage is that a series of such slices can be reconstructed to give 3D views and enable volume analysis of the sample, and that any one slice 作者: 灰姑娘 時(shí)間: 2025-3-29 02:49 作者: 安慰 時(shí)間: 2025-3-29 05:47
detection methods (.). However, this method of detection until recently has been limited by the required use of alkaline phosphatase conjugated secondary antibodies and chromogenic substrates. The use of alkaline phosphatase substrates and their diffusible products limits the resolution of staining作者: chondromalacia 時(shí)間: 2025-3-29 11:09 作者: Arbitrary 時(shí)間: 2025-3-29 14:46 作者: hysterectomy 時(shí)間: 2025-3-29 18:01 作者: FRAX-tool 時(shí)間: 2025-3-29 23:32
st cells produced by animals, and sperm are frequently the smallest. Observation of the initial steps of fertilization, including sperm-egg binding and fusion, can be challenging owing to the size discrepancy between these two cell types and, in many species, the rapid time course over which they oc作者: hypertension 時(shí)間: 2025-3-30 01:55
zation in developing embryos. For . researchers, the ease of generating such antisera (.) and the number and widespread availability of existing antibodies make immunofluroescence of embryos an indispensable technique. The use of fluorochrome-conjugated secondary and/or tertiary antibodies on . embr作者: cardiopulmonary 時(shí)間: 2025-3-30 07:30
which prevents frozen sectioning because the yolk crystallizes and tears the sections. In addition, the yolk autofluoresces, making whole-mount immunocytochemistry possible, but difficult due to background from out-of-focus fluoresecence. All of these problems can be solved through confocal microsc作者: excrete 時(shí)間: 2025-3-30 09:32 作者: 切掉 時(shí)間: 2025-3-30 13:24 作者: Crepitus 時(shí)間: 2025-3-30 17:45
t advances have also occurred in the development of reagents and techniques for generating functional fluorescently tagged proteins. When these labeled proteins are used in conjunction with confocal and other advanced fluorescence microscopes, the dynamics of a given protein within the living cell c作者: Instinctive 時(shí)間: 2025-3-30 23:53
es. First, it would function in living animals, eliminating the need for fixation and dehydration and their associated artefacts. Second, it would permit each stage of development to be studied in a single, intact embryo. Third, it would function in all cell types and would reveal their morphology, 作者: Synovial-Fluid 時(shí)間: 2025-3-31 02:46 作者: 一致性 時(shí)間: 2025-3-31 08:45 作者: LAY 時(shí)間: 2025-3-31 09:21
lume regulation involves the removal of cells from the matrix and manipulation in culture (.). Measurements are often also needed from cells within intact tissue that are operating in their correct physiological context. These include the effects of cell-cell interactions and the mechanical, ionic, 作者: MAIM 時(shí)間: 2025-3-31 15:41
ence, and that do not strongly scatter or absorb either the excitation or emission light. This chapter provides protocols used to examine three such tissues: the cornea of the eye, the buccal mucosa (which lines the inner cheek), and the nasal respiratory epithelium. Although in each case our overal作者: Atrium 時(shí)間: 2025-3-31 19:48 作者: enlist 時(shí)間: 2025-4-1 00:31
Confocal Microscopy978-1-59259-722-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Benzodiazepines 時(shí)間: 2025-4-1 03:09
This chapter differs from others in this volume in that it does not describe protocols .. Rather, it consists of checklists, cautions, tips, rules of thumb, and advice related to fluorescent probes in general and probes for confocal microscopy in particular.
