標(biāo)題: Titlebook: Computational Design of Ligand Binding Proteins; Barry L. Stoddard Book 2016 Springer Science+Business Media New York 2016 ligand-binding [打印本頁] 作者: Kennedy 時(shí)間: 2025-3-21 16:40
書目名稱Computational Design of Ligand Binding Proteins影響因子(影響力)
書目名稱Computational Design of Ligand Binding Proteins影響因子(影響力)學(xué)科排名
書目名稱Computational Design of Ligand Binding Proteins網(wǎng)絡(luò)公開度
書目名稱Computational Design of Ligand Binding Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Computational Design of Ligand Binding Proteins被引頻次
書目名稱Computational Design of Ligand Binding Proteins被引頻次學(xué)科排名
書目名稱Computational Design of Ligand Binding Proteins年度引用
書目名稱Computational Design of Ligand Binding Proteins年度引用學(xué)科排名
書目名稱Computational Design of Ligand Binding Proteins讀者反饋
書目名稱Computational Design of Ligand Binding Proteins讀者反饋學(xué)科排名
作者: Perceive 時(shí)間: 2025-3-21 21:30
Zusammenfassung der Ergebnisse,eve this is the theozyme placement and enzyme design algorithms implemented in Rosetta Molecular Modeling Suite. Here, we illustrate how to use this software to recapitulate the placement of catalytic residues and ligand into a protein using a theozyme, protein scaffold, and catalytic constraints as input.作者: 歹徒 時(shí)間: 2025-3-22 04:07
Barry L. StoddardIncludes cutting-edge methods and protocols.Provide step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary material作者: 細(xì)節(jié) 時(shí)間: 2025-3-22 04:38
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/232231.jpg作者: 手工藝品 時(shí)間: 2025-3-22 11:43 作者: outer-ear 時(shí)間: 2025-3-22 15:05
978-1-4939-8080-2Springer Science+Business Media New York 2016作者: outer-ear 時(shí)間: 2025-3-22 18:55 作者: CYT 時(shí)間: 2025-3-22 22:00
Verschlüsselung und Substanz-Verzeichnisse–ligand interactions. However, most of the currently available computational techniques are affected by severe deficiencies in both protein and ligand conformational sampling as well as in the scoring of the obtained docking solutions. To overcome these limitations, we have recently developed Medusa作者: 合并 時(shí)間: 2025-3-23 02:29
Die biologische Funktion der Steroidhormone,des, respectively. GalaxySite predicts ligands that may bind the input protein and generates complex structures of the protein with the predicted ligands from the protein structure given as input or predicted from the input sequence. GalaxyPepDock takes a protein structure and a peptide sequence as 作者: Parallel 時(shí)間: 2025-3-23 08:14
Struktur und Nomenklatur der Steroide,for a particular target ligand are not available, artificial proteins can be computationally designed. We present a protocol based on RosettaLigand to redesign an existing protein pocket to bind a target ligand. Starting with a protein structure and the structure of the ligand, Rosetta can optimize 作者: 神圣在玷污 時(shí)間: 2025-3-23 12:18 作者: harrow 時(shí)間: 2025-3-23 17:33 作者: ectropion 時(shí)間: 2025-3-23 21:26 作者: 幾何學(xué)家 時(shí)間: 2025-3-24 00:13 作者: 聯(lián)合 時(shí)間: 2025-3-24 04:05
Zusammenfassung der Ergebnisse, protein design with the high-throughput screening of mutagenic libraries of computationally designed proteins is emerging as a general approach for creating binding proteins with programmable binding modes, affinities, and selectivities. The computational step enables the creation of a binding site作者: ARK 時(shí)間: 2025-3-24 08:16 作者: BYRE 時(shí)間: 2025-3-24 12:16 作者: 星球的光亮度 時(shí)間: 2025-3-24 16:26
Zusammenfassung der Ergebnisse,luding spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two examples from the literature: the e作者: florid 時(shí)間: 2025-3-24 22:08 作者: Agronomy 時(shí)間: 2025-3-25 00:13
Zusammenfassung der Ergebnisse,a large number of peptide ligands for a protein target. In this chapter, we describe a procedure called “SORTCERY” that can rank the affinities of library members for a target with high accuracy. SORTCERY follows a three-step protocol. First, fluorescence-activated cell sorting (FACS) is used to sor作者: 不能強(qiáng)迫我 時(shí)間: 2025-3-25 04:14 作者: 責(zé)難 時(shí)間: 2025-3-25 11:19
Steuer-ABC des Wertpapierbesitzers technologies. It has been well established that computational design to accommodate small numbers of DNA target site substitutions is possible. This chapter details the basic method of design used in the Rosetta macromolecular modeling program that has been successfully used to modulate the specifi作者: narcotic 時(shí)間: 2025-3-25 13:21
Steuer-ABC des Wertpapierbesitzersractions is of major interest for biomedical and synthetic biology applications, as well as to address fundamental biological questions. In recent years, computational protein design has emerged as a tool for designing novel protein–protein interactions with functional relevance. Although attractive作者: 異端 時(shí)間: 2025-3-25 18:28 作者: Prostatism 時(shí)間: 2025-3-25 20:24
https://doi.org/10.1007/978-3-663-13028-4of TCR–pMHC complexes have provided enormous insight to cellular immune functions, permitted a rational understanding of processes such as pathogen escape, and led to the development of novel approaches for the design of vaccines and other therapeutics. As production, crystallization, and structure 作者: 蜈蚣 時(shí)間: 2025-3-26 03:21 作者: 陪審團(tuán) 時(shí)間: 2025-3-26 06:06 作者: 凹槽 時(shí)間: 2025-3-26 10:11 作者: Conquest 時(shí)間: 2025-3-26 16:12
Zusammenfassung der Ergebnisse,ch where the binding mode is preprogrammed and iteratively refined, selectivity can be achieved (and modulated) by the placement of specified amino acid side chain groups around the ligand in defined orientations. Here, we describe the experimental aspects of a combined computational–experimental ap作者: 有其法作用 時(shí)間: 2025-3-26 20:36 作者: insidious 時(shí)間: 2025-3-26 21:40 作者: LEER 時(shí)間: 2025-3-27 02:31 作者: insightful 時(shí)間: 2025-3-27 06:26
https://doi.org/10.1007/978-3-663-13028-4how how the approach can recapitulate changes in TCR binding angles and other structural details, and highlight areas where careful evaluation of parameters is needed and alternative choices might be made. As TCRs are highly sensitive to subtle structural perturbations, there is room for improvement作者: 軍火 時(shí)間: 2025-3-27 09:29 作者: Medicaid 時(shí)間: 2025-3-27 14:31 作者: Eosinophils 時(shí)間: 2025-3-27 18:35 作者: 糾纏 時(shí)間: 2025-3-28 00:26
Improving Binding Affinity and Selectivity of Computationally Designed Ligand-Binding Proteins Usinch where the binding mode is preprogrammed and iteratively refined, selectivity can be achieved (and modulated) by the placement of specified amino acid side chain groups around the ligand in defined orientations. Here, we describe the experimental aspects of a combined computational–experimental ap作者: neurologist 時(shí)間: 2025-3-28 02:31
Computational Design of Multinuclear Metalloproteins Using Unnatural Amino Acids,The method requires a target symmetric organometallic cofactor whose coordinating ligands resemble the side chains of a natural or unnatural amino acid and a library of oligomeric protein structures featuring the same symmetry as the target cofactor. Geometric interface matches between target cofact作者: gnarled 時(shí)間: 2025-3-28 10:06 作者: surrogate 時(shí)間: 2025-3-28 13:02 作者: Judicious 時(shí)間: 2025-3-28 17:30 作者: Malleable 時(shí)間: 2025-3-28 20:21 作者: 我沒有命令 時(shí)間: 2025-3-28 23:45 作者: jarring 時(shí)間: 2025-3-29 05:51
https://doi.org/10.1007/BFb0091063ering laboratories willing to think beyond site saturation mutagenesis. In the common case that the exact experimental screening budget is not fixed, it is particularly helpful to perform a Pareto analysis to inspect favorable libraries at a range of possible library sizes.作者: 油膏 時(shí)間: 2025-3-29 11:15
https://doi.org/10.1007/978-3-642-99124-0for constructing redox active sites with either the same (e.g., 4Cys) or mixed (e.g., 2HisCys) ligands, a feat that could be achieved in this preassembled framework. Here, we describe the process of constructing MBSs in α.D and our expression techniques.作者: 捕鯨魚叉 時(shí)間: 2025-3-29 13:28 作者: 高爾夫 時(shí)間: 2025-3-29 15:46
Reform des Direkten Steuersystems,ed the method to design peptides that discriminate with high selectivity between two closely related PDZ domains. The framework accounts for the flexibility of the peptide in the binding site, but is efficient enough to quickly analyze trade-offs between affinity and selectivity, enabling the identification of optimal peptides.作者: Calculus 時(shí)間: 2025-3-29 21:20
Computational Modeling of Small Molecule Ligand Binding Interactions and Affinities,. Here, we present a standard computational protocol to evaluate the binding properties of the two enantiomers of the non-selective β-blocker propanolol in the β2 adrenergic receptor’s binding site. We describe details of our protocol, which have been successfully applied to several other targets.作者: 儲(chǔ)備 時(shí)間: 2025-3-30 01:04 作者: Detonate 時(shí)間: 2025-3-30 06:50 作者: 否認(rèn) 時(shí)間: 2025-3-30 09:19 作者: Exhilarate 時(shí)間: 2025-3-30 16:16 作者: Small-Intestine 時(shí)間: 2025-3-30 16:47
,Design of Specific Peptide–Protein Recognition,ed the method to design peptides that discriminate with high selectivity between two closely related PDZ domains. The framework accounts for the flexibility of the peptide in the binding site, but is efficient enough to quickly analyze trade-offs between affinity and selectivity, enabling the identification of optimal peptides.作者: 寬敞 時(shí)間: 2025-3-31 00:07 作者: FRET 時(shí)間: 2025-3-31 04:49
Het afleggen, de uitvaart en de begrafenis,zer has been tested in a comprehensive benchmark and predicted mutations have also been used in experimental tests. In this chapter, we will provide general recommendations for usage as well as an in-depth description of all individual PocketOptimizer modules.作者: 散布 時(shí)間: 2025-3-31 06:16
Zusammenfassung der Ergebnisse,t a library of yeast-displayed peptide ligands according to their affinities for a target. Second, all sorted pools are deep sequenced. Third, the resulting data are analyzed to create a ranking. We demonstrate an application of SORTCERY to the problem of ranking peptide ligands for the anti-apoptotic regulator Bcl-x..作者: 變形詞 時(shí)間: 2025-3-31 12:59
Steuer-ABC des Wertpapierbesitzers examples of ROSETTA computational protocols for the design of functional protein binders using seeded protein interface design. In these protocols, a motif of known structure that interacts with the target site is grafted into a scaffold protein, followed by design of the surrounding interaction surface.作者: neutral-posture 時(shí)間: 2025-3-31 17:14
,Motif-Driven Design of Protein–Protein Interfaces, examples of ROSETTA computational protocols for the design of functional protein binders using seeded protein interface design. In these protocols, a motif of known structure that interacts with the target site is grafted into a scaffold protein, followed by design of the surrounding interaction surface.作者: blithe 時(shí)間: 2025-3-31 21:06
1064-3745 tion advice from the experts.Includes supplementary material.This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today‘s leaders in the field ofprotein engineering. Chapters focus on?modeling protein ligand 作者: Inculcate 時(shí)間: 2025-3-31 22:23
Struktur und Nomenklatur der Steroide, redesign an existing protein pocket to bind a target ligand. Starting with a protein structure and the structure of the ligand, Rosetta can optimize both the placement of the ligand in the pocket and the identity and conformation of the surrounding sidechains, yielding proteins that bind the target compound.作者: agitate 時(shí)間: 2025-4-1 05:07
Struktur der erzeugenden Distributionen, computational design, iterative site saturation mutagenesis, and yeast two-hybrid system (Y2H)-based phenotypic screening to engineer novel and orthogonal interactions between synthetic ligands and human estrogen receptor α (hERα) for the development of novel gene switches.作者: Conscientious 時(shí)間: 2025-4-1 09:40
Rosetta and the Design of Ligand Binding Sites, redesign an existing protein pocket to bind a target ligand. Starting with a protein structure and the structure of the ligand, Rosetta can optimize both the placement of the ligand in the pocket and the identity and conformation of the surrounding sidechains, yielding proteins that bind the target compound.
